Team:Gothenburg/Calendar

From 2014.igem.org

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                         <li>Colony PCR</li>
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                         <li>Title two</li>
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                         <li>Colony PCR</li>
                         <li>Colony PCR</li>
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                         <li>Title two</li>
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Latest revision as of 03:01, 14 October 2014

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June

26

27

28

29

30

31

1

2

3

4

5

6

7

8

9

10

  • Safety Instructions

11

12

13

14

15

16

17

  • Introduction to centrifuges and autoclaves

18

19

20

  • Finished mandatory training

21

22

23

  • Media Preparation

24

25

  • Amplification and Purification of non-synthetic parts

26

  • Plasmid Purification
  • Amplification and purification of parts

27

  • Gel extraction
  • PCR amplification and merging

28

  • PCR amplification and merging
  • Plasmid restriction

29

30

  • PCR amplification and merging

1

  • Plasmid restriction
  • PCR amplification

2

  • PCR amplification
  • Gel extraction
  • Concentration determination

3

4

  • Plasmid Purification
  • Plasmid restriction
  • Gel extraction

5

  • Concentration determination

6

July

  • PCR amplification and merging
  • 1

    • Plasmid restriction
    • PCR amplification

    2

    • PCR amplification
    • Gel extraction
    • Concentration determination

    3

    4

    • Plasmid Purification
    • Plasmid restriction
    • Gel extraction

    5

    • Concentration determination

    6

    7

    • Plasmid restriction

    8

    • Plasmid restriction

    9

    • Plasmid restriction

    10

    • Plasmid restriction
    • Gel extraction

    11

    12

    13

    14

    15

    16

    17

    • PCR amplification and merging
    • Gel extraction

    18

    • PCR amplification and merging
    • Gel extraction
    • Dissolution of synthetic parts

    19

    20

    21

    22

    • Amplification and purification of synthetic parts
    • Gel extraction
    • Preparation of competent cells

    23

    • Amplification and purification of synthetic parts
    • Gel extraction
    • Yeast transformation

    24

    • Plasmid restriction

    25

    • Dissolution of synthetic parts
    • Amplification of synthetic parts
    • PCR purification
    • Concentration determination

    26

    27

    28

    • Yeast Transformation

    29

    30

    • Observation of the previously transformed cells
    • Plasmid restriction
    • Preparation of Competent cells

    31

    • Inoculation of cells

    1

    • Yeast transformation
    • Inoculation of cells

    2

    3

    Augusti

    28

    • Yeast Transformation

    29

    30

    • Observation of the previously transformed cells
    • Plasmid restriction
    • Preparation of Competent cells

    31

    • Inoculation of cells

    1

    • Yeast transformation
    • Inoculation of cells

    2

    3

    4

    • PCR amplification and merging
    • Gel extraction
    • Plasmid restriction

    5

    • Plasmid restriction
    • Gel extraction
    • Concentration determination
    • PCR amplification and merging

    6

    • PCR amplification and merging
    • Gibson Assembly

    7

    • Plasmid Purification
    • Gel extraction
    • Gibson Assembly

    8

    • Observed results from the Gibson Assembly
    • Yeast Transformation

    9

    10

    11

    • PCR amplification and merging
    • E. coli transformation

    12

    • Diagnostic gel and gel extraction
    • E. coli transformation

    13

    • Concentration determination
    • Yeast Electroporation

    14

    • Yeast electroporation

    15

    • Plasmid Purification
    • PCR amplification and merging

    16

    17

    18

    • Diagnostic gel
    • PCR purification
    • Plasmid restriction
    • Gel extraction

    19

    • Gibson Assembly
    • Yeast transformation

    20

    • Colony PCR

    21

    22

    • Plasmid Purification
    • Title two

    23

    • Fluorescence microscopy
    • PCR amplification
    • Gel extraction

    24

    • PCR amplification
    • Concentration determination
    • Yeast plasmid extraction

    25

    • Restriction digestion

    26

    • Restriction digestion
    • Gel extraction
    • Concentration determination

    27

    • Yeast Transformation
    • E. coli transformation

    28

    • Inoculation of plates

    29

    • Colony PCR

    30

    31

    September

    1

    • Restriction digestion

    2

    3

    4

    5

    6

    7

    8

    9

    10

    11

    12

    13

    14

    15

    16

    17

    18

    19

    20

    21

    22

    23

    24

    25

    26

    27

    28

    29

    30

    1

    2

    3

    4

    5