Team:Toulouse/Result/parts

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   <div id="innercontenthome">
   <div id="innercontenthome">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
-
 
-
 
        
        
-
 
+
<p class="texte">As the chassis <i>Bacillus subtilis</i> is not as used as <i>Escherichia coli</i>,  
-
<p class="texte">As the chassis <i>B. subtilis</i> is not as used as <i>E. coli</i>,  
+
it was sometimes hard for us to get or find desired parts.<br>
it was sometimes hard for us to get or find desired parts.<br>
-
However, in collaboration with other iGEM teams and with a synthesis compagny, we managed to obtain or make the following parts:<br>
+
However, in collaboration with other iGEM teams and with a synthesis company, we managed to obtain or make the following parts:<br>
-
</p>
+
-
 
+
-
<p class="title1">Submitted parts</p>
+
-
 
+
-
<p class="texte">
+
-
We have deposed 16 new BioBrick parts to the Registry.
+
-
All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.<br>
+
-
<!--à vérifier et confirmer-->
+
-
</p>
+
-
 
+
-
 
+
-
<p class="title2">Chemotaxis</p>
+
-
 
+
-
<!--K1364000 : RBS + Chimio + terminator-->
+
-
 
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a>:N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i><br></p>
+
-
<p class="texte">This part is designed to enable a N-acetylglucosamine
+
-
based chemotaxis in <i>Bacillus subtilis</i>.
+
-
<p class="title4">Design</p>
+
-
<p class="texte">The encoded protein is a chimera of two proteins:<br>
+
-
- the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
+
-
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from <i>Vibrio cholerae</i> (VCD).<br>
+
-
<br>
+
-
The chimeric protein contains the intracellular domains of McpA from <i>Bacillus subtilis</i> to enable the transduction of the signal:
+
-
these domains correspond to the amino acids 1-37 and 282-610.
+
-
The extracellular domain of VCD is inserted between the intracellular regions of McpA
+
-
to sense N-acetylglucosamine and corresponds to the amino acids 31-310.<br></p>
+
-
<img src="https://static.igem.org/mediawiki/2014/a/a2/BBa_K1364000.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translation unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part is not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Uniprot:<br>
+
-
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
+
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br></p>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364004 : Promotor Pveg + Chimio-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a>: P<sub>veg</sub> + N-acetylatedglucosamine based chemotaxis for <i>B. subtilis</i>
+
-
<br>
+
-
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,
+
-
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
+
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
-
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of D4E1
+
-
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/3/35/BBa_K1364004.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Uniprot:<br>
+
-
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
+
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br>
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title2">Binding</p>
+
-
 
+
-
<!--K1364001 : RBS + Binding + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>:  P<sub>veg</sub> + Chitin Binding Cell Wall protein</p>
+
-
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of a Cell Wall Binding (CWB) sequence
+
-
and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.<br>
+
-
<br>
+
-
The CWB domain has been extracted from LytC (<a href="http://parts.igem.org/Part:BBa_K316030">BBa_K316030</a>) of the iGEM 2010 Imperial College team.<br>
+
-
The CBD is composed of the Domain 4 of the GbpA protein of <i>Vibrio cholerae</i>,
+
-
a protein reported to mediate bacterial attachment to chitin.
+
-
It has been shown that the Domain 4 was essential to bind chitin.</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/f/f5/BBa_K1364005.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested with chitin beeds. (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Binding module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
 
+
-
<p class="title2">Fungicides</p>
+
-
<p class="title3">GAFP-1</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364002 : RBS + GAFP-1-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</p>
+
-
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
+
-
originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
-
GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/5/5f/BBa_K1364002.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translational unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
+
-
<br>
+
-
 
+
-
<!--K1364007 : RBS + GAFP-1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</p>
+
-
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
+
-
originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
-
GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/4/42/BBa_K1364007.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite part</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
+
-
<br>
+
-
 
+
-
<!--K13640008 : Pveg + RBS + GAFP-1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p>
+
-
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
+
-
originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
-
GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>,
+
-
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/6/67/BBa_K1364008.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title3">D4E1</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364003 : RBS + D4E1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</p>
+
-
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids
+
-
which has shown to have antifungal activities
+
-
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of D4E1 and
+
-
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
-
thanks to the DNA 2.0 software program.</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/b/bb/BBa_K1364003.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translational unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
+
-
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
+
-
 
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364009 : Pveg + RBS + D4E1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p>
+
-
<p class="texte">
+
-
D4E1 is a linear synthetic peptide of 17 amino acids
+
-
which has shown to have antifungal activities
+
-
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
-
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of D4E1 and
+
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
+
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
-
thanks to the DNA 2.0 software program.</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/4/47/BBa_K1364009.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translational unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
+
-
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
+
-
<br>
+
-
 
+
-
<p class="title3">EcAMP-1</p>
+
-
 
+
-
<!--K1364010 : Pveg RBS + EcAMP -->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</p>
+
-
<p class="texte">
+
-
EcAMP-1 is an antimicrobial peptide of 37 amino acids
+
-
originated from the specie <i>Echinochloa crus-galli</i>,
+
-
a type of wild grass.
+
-
This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
+
-
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
+
-
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>) and
+
-
the open reading frame of EcAMP-1.<br>
+
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
-
and thanks to the Life Technologies GeneArt software program. </p>
+
-
<img src="https://static.igem.org/mediawiki/2014/5/5f/BBa_K1364010.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte"> Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part is not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte"><!--à compléter : demander à emeline--></p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364011 : Pveg RBS + EcAMP + Double term-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
+
-
<p class="texte">
+
-
EcAMP-1 is an antimicrobial peptide of 37 amino acids
+
-
originated from the specie <i>Echinochloa crus-galli</i>,
+
-
a type of wild grass.
+
-
This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
+
-
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
+
-
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
-
the open reading frame of EcAMP-1 and
+
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
-
and thanks to the Life Technologies GeneArt software program. </p>
+
-
<img src="https://static.igem.org/mediawiki/2014/7/74/BBa_K1364011.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part is not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte"><!--à compléter : demander à emeline--></p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title3">Fungicide operons</p>
+
-
+
-
<!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
+
-
<p class="texte">
+
-
<p class="title4">Design</p>
+
-
<p class="texte">This part is designed for the co-expression of two different peptides
+
-
with anti-fungal activities : D4E1 and GAFP-1.
+
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)
+
-
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
+
-
<img src="https://static.igem.org/mediawiki/2014/4/40/BBa_K1364013.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
+
-
<br>
+
-
<p class="title2">Basic tools</p>
+
-
 
+
-
<!--K1364015-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Reporter</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364016-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Reporter</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364017-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364019-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> + RBS + Antifungal EcAMP + Double terminator</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364021-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--SOUS-TITRE-->
+
-
<p class="title1">Used parts</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--Table-->
+
-
<p class="texte"><center>
+
-
<table border="1" width="100%" height="20%">
+
-
<!--1ere ligne-->
+
-
<tr><td><p class="texte"><b>BioBrick</b></p></td>
+
-
<td><p class="texte"><b>Description</b></p></td>
+
-
<td><p class="texte"><b>Origine</b></p></td>
+
-
<td><p class="texte"><b>Module(s)</b></p></td></tr>
+
-
<!--2eme ligne-->
+
-
<tr><td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K733013">K733013</a></p></td>
+
-
<td><p class="texte">P<sub>veg</sub> (<a href="http://parts.igem.org/Part:BBa_K316001">K316001</a>) + RBS-SpoVG (<a href="http://parts.igem.org/Part:BBa_K143021">K143021</a>) : Constitutive promotor P<sub>veg</sub> and strong RBS for <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">2014 Kit plate 1, 7H</p></td>
+
-
<td><p class="texte">Fungicides (EcAMP)</p></td>
+
-
</tr>
+
-
<!--3eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823003">K823003</a></p></td>
+
-
<td><p class="texte">P<sub>veg</sub> : strong, constitutive promotor of <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">2014 Kit plate 1, 20G</p></td>
+
-
<td><p class="texte">Fungicides (D4E1 GAFP-1)<br>Chemotaxis<br>Binding</p></td>
+
-
</tr>
+
-
<!--4eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823002">K823002</a></p></td>
+
-
<td><p class="texte">P<sub>lepA</sub> : constitutive promotor of <i>B. subtilis</i>.
+
-
<br>It is the promotor of the lepA gene of <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">2014 Kit plate 1, 20E</p></td>
+
-
<td><p class="texte">Fungicides (D4E1)<br>Chemotaxis<br>Binding</p></td>
+
-
</tr>
+
-
<!--5eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K606061">K606061</a></p></td>
+
-
<td><p class="texte">SpoVG RBS for <i>B. subtilis</i>
+
-
<br>Strong ribosome binding site from subtilis. Works fine in <i>E. coli</i></p></td>
+
-
<td><p class="texte">2014 Kit plate 1, 15A</p></td>
+
-
<td><p class="texte">Optional</p></td>
+
-
</tr>
+
-
<!--6eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K316027">K316027</a></p></td>
+
-
<td><p class="texte"><i>B. subtilis</i> transformation vector with LacI, targets amyE locus.</p></td>
+
-
<td><p class="texte">2014 Kit plate 1, 2N</p></td>
+
-
<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
+
-
</tr>
+
-
<!--7eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_B0015">B0015</a></p></td>
+
-
<td><p class="texte">Double terminator (<a href="http://parts.igem.org/Part:BBa_B0010">BBa_B0010</a> + <a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a>)</p></td>
+
-
<td><p class="texte">2014 Kit plate 3, 3F</p></td>
+
-
<td><p class="texte">Fungicides (GAFP-1, EcAMP-1)</p></td>
+
-
</tr>
+
-
<!--8eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780000">K780000</a></p></td>
+
-
<td><p class="texte">Terminator for <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
+
-
<td></td>
+
-
</tr>
+
-
<!--9eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780001">K780001</a></p></td>
+
-
<td><p class="texte">Strong RBS for <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
+
-
<td></td>
+
-
</tr>
+
-
<!--10eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780002">K780002</a></p></td>
+
-
<td><p class="texte">Strong RBS for <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
+
-
<td></td>
+
-
</tr>
+
-
<!--11eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780003">K780003</a></p></td>
+
-
<td><p class="texte">Strong constitutive promotor for <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
+
-
<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
+
-
</tr>
+
-
<!--12eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a></p></td>
+
-
<td><p class="texte">EcAMP-1</p></td>
+
-
<td><p class="texte">Sent by the Utah State iGEM team</p></td>
+
-
<td><p class="texte">Fungicides (EcAMP-1)</p></td>
+
-
</tr>
+
-
<!--13eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823023">K823023</a></p></td>
+
-
<td><p class="texte">Empty backbone vector pSB<sub>BS</sub>1C for integration into <i>B. subtilis</i>
+
-
<br>It integrates in the amyE locus</p></td>
+
-
<td><p class="texte">Sent by the Munich iGEM team</p></td>
+
-
<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td></tr>
+
-
<!--14eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823022">K823022</a></p></td>
+
-
<td><p class="texte">Empty backbone vector pSB<sub>BS</sub>4S for integration into<i>B. subtilis</i>
+
-
<br>It integrates in the thrC locus</p></td>
+
-
<td><p class="texte">Sent by the Munich iGEM team</p></td>
+
-
<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
+
-
</tr>
+
-
<!--15eme ligne-->
+
-
<tr>
+
-
<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823021">K823021</a></p></td>
+
-
<td><p class="texte">Reporter vector pSB<sub>BS</sub>1C-lacZ for <i>B. subtilis</i></p></td>
+
-
<td><p class="texte">Sent by the Munich iGEM team</p></td>
+
-
<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td></tr>
+
-
</table></center>
+
</p>
</p>
 +
<ul>
 +
<li class="tree"><p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts"><font color="green">Submitted parts</p></a></li>
 +
<li class="tree"><p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Used_parts"><font color="green">Used parts</p></a></li>
 +
</ul>
-
<br>
+
</div>
 +
<div class="clear"></div>
      
      
   <!-- Navigation section -->   
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   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
-
     <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Experimental results
+
     <a href="https://2014.igem.org/Team:Toulouse/Modelling" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Modeling
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
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     </a>  
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     <a href="https://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
-
color:#666; font-size:18px;">Achievement</br>
+
color:#666; font-size:18px;">Achievements</br>
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
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Latest revision as of 03:55, 18 October 2014

           
        logo-igem            

Parts

What did we send to the Registry?

Results   >   Parts

As the chassis Bacillus subtilis is not as used as Escherichia coli, it was sometimes hard for us to get or find desired parts.
However, in collaboration with other iGEM teams and with a synthesis company, we managed to obtain or make the following parts:

Modeling
logo
Achievements

iGEM Toulouse 2014

Retrieved from "http://2014.igem.org/Team:Toulouse/Result/parts"