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Michigantest
From 2014.igem.org
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Latest revision as of 19:09, 13 October 2014
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2014 Michigan Synthetic Biology Team
MSBT
University of Michigan iGEM Team
In our project, we've purified antibody fragments by tagging a secretion protein, OsmY, to an antibody scFv (single chain fragment variable) and over-expressing the construct in E.coli. To demonstrate the importance of our study we've chosen an antibody specific to DADH, a Salmonella protein. Salmonella is responsible for 23,000 hospitalizations in the US each year, highlighting the need for large quantities of inexpensive sensors. To further demonstrate the value of our part we've compared our secretion system with industrially used secretion system that use the secretion tag pelB.
In our project, we've purified antibody fragments by tagging a secretion protein, OsmY, to an antibody scFv (single chain fragment variable) and over-expressing the construct in E.coli. To demonstrate the importance of our study we've chosen an antibody specific to DADH, a Salmonella protein. Salmonella is responsible for 23,000 hospitalizations in the US each year, highlighting the need for large quantities of inexpensive sensors. To further demonstrate the value of our part we've compared our secretion system with industrially used secretion system that use the secretion tag pelB.
