Team:UT-Tokyo/CTCD/Content

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Latest revision as of 03:02, 18 October 2014 (view source)

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<li><a href="Javascript:loadContent('Achievement-block','Achievement-1')">Judging Form</a></li>

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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">~~Introduction~~</a></li>

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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">Human Practice</a></li>

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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">~~To spread iGEM in general public</a></li>~~

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<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">Ethics</a></li>

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~~<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-3')">To activate iGEM~~</a></li>

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~~<div id = "Project-1">~~

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~~<img src="https://static.igem.org/mediawiki/2014/d/d9/SubCTC_introduction.png" class = "contTitle" />~~

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<p>Please imagine that you are a police officer. You are searching for dangerous criminals who hide in crowd and it is difficult to find them. This situation is the same as finding cancer cells (dangerous criminals in our body) in our blood when they resemble to normal blood cells. In this way picking up cancer cells has been developed around the world, where every year a different method is developed. Don't you think that it is like a dream if there were drugs, which if someone take, they will confess their crime? What about drugs, which if the cancer cells take, they will shine brightly on the blood? Here our team focuses on microRNA (miRNA) and provides a special genetic circuit which exposes cancer cells in blood vessels.(Fig.1)</p>

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~~<img src="https://static.igem.org/mediawiki/2014/f/fa/Introduction_CTC_fig1.png" class ="figure" />~~

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<p>Circulating tumor cells (CTCs) tumor cells arising from epithelia, flowing through blood vessels and causing metastasis to other tissues. Focusing on these cells, there are ways of early detection of cancer, detecting CTCs in blood of patients with early-stage cancer. The effectiveness of these methods has been ensured through the results of researches especially in breast cancer and colon cancer (ref). These methods of detection are a technology developed in recent years, compared to the traditional methods of picking up the tissue of patients which has been used for a long time. However, these traditional methods produce a hard burden to the patients, because of the several and long procedures. On the other hand, the methods of detecting CTCs produce a non-invasive option for patients compared to traditional ones. From the utility points, CTCs have been used all around the world to develop several detection methods. Most of these methods emphasized utilizing anti-EpCAM antibody, which is an adhesion protein used as marker of cancers, and distinguish CTCs from blood cells by the difference of their cell size. However, because the concentration of CTCs in blood is very low, it is required to develop a new approach for the detection of CTCs.</p>

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<p>Here we have developed a new detection method for CTCs focusing on two types of profiles; miRNA and EpCAM. It has given the more specificity for CTCs to utilize the two profiles. miRNA is a special short non-cording RNA and interferes the translation of the complementary mRNA, interacting with some proteins and forming the RNA-induced silencing complex (RISC) in wide-species from virus to human. Focusing on different expression patterns of miRNA in each cell type, we utilized miRNA profiles with a synthetic biological approach for detecting CTCs. The circuit that we have developed has EpCAM promoter, reporter and blood cells-specific miRNA (<i>miR-142-3p/5p</i>) binding sites. Two conditions, which are activation of EpCAM promoter and an absence of blood cells-specific miRNA, are required for translation of the reporter. If we inject this circuit into cells in blood, only in the case of CTCs the reporter is produced.(Fig.2)</p>

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~~<img src="https://static.igem.org/mediawiki/2014/1/13/Introduction_CTC_fig2.png" class ="figure" />~~

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~~<p>[1] Friedlander, Terence W., Gayatri Premasekharan, and Pamela L. Paris. "Looking back, to the future of circulating tumor cells." <i>Pharmacology & therapeutics</i> 142.3 (2014): 271-280.</p>~~

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~~</div>~~

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~~<div id = "Project-2">~~

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~~<img src = "https://static.igem.org/mediawiki/2014/8/8f/SubCTC_system.png" class = "contTitle"/>~~

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~~<img src="https://static.igem.org/mediawiki/2014/6/64/Kobari_pegp2.png" class = "figure" />~~

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~~<legend style="margin-left:300px;"><b>Fig.3</b> the construct of our circuit</legend>~~

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~~<h3>EGP-2 promoter</h3>~~

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<p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.EpCAM is a trans membrane glycoprotein which is expressed in epithelial cells and shows high level expression in various type of human epithelial carcinomas[2].Using this promoter, reporter genes can be expressed only in cancer cells. EGP-2 promoter is originally about 3500bp and it is not convenient to make a DNA construct. But, in the previous research, it is known that EGP-2 promoter can work if a part of its sequence is deleted[3]. In this project ,We cloned a part of EGP-2 promoter, and made it useful as a biobrick parts.</p>

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~~<h3>miRNA-142</h3>~~

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<p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[4].After miRNA-142 is transcribed, it is cleaved to miRNA-142-5p and miRNA-142-3p. Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.</p>

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~~<img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" />~~

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<legend><b>Fig.4</b> When this construct is transfected to the epithelial cells like cancer cell, pEGP-2 promote the transcription of EGFP, and the mRNA is not degraded by miRNA because miR 142 is specific to hematopoietic cells.</legend>

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~~<img src="https://static.igem.org/mediawiki/2014/4/44/Kobari_hematopoietic_cell.png" class = "figure" />~~

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<legend><b>Fig.5</b> When this construct is transfected to the hematopoietic cells, pEGP-2 does not work as a promoter, and mRNA is not transcribed. Even if the leak of the promoter makes few mRNA ,this mRNA is degraded by RNAi caused by miR 142.</legend>

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~~<div id = "Project-3">~~

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~~<img src = "https://static.igem.org/mediawiki/2014/b/b4/SubCTC_application.png" class = "contTitle" />~~

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<p>In our project, miRNA is used for degrading mRNA in cells that express miRNA. But if we transfect construct like [pCMV-LacI-miR A binding site] and [pCAG-LacO2-GFP][5], mRNA in cells that does not express miRNA is degraded. This system will makes many application of miRNA in iGEM possible.</p>

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<p>We focused on a tissue-specific promoter and miRNA in order to detect CTCs.As well as hematopoietic cells, many other tissues shows specific miRNA expression patterns[3]. Therefore, if by making a circuit that returns an output to a certain miRNA expression pattern, we may identify where a CTC comes from.[5]</p>

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<p>In addition to the detection of CTCs, combinations of tissue-specific promoter and miRNA can be applied to many treatments. For example, suicide genes can be expressed in cancer cells in a specific tissue.[6]</p>

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~~<div id = "Safety-1">~~

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~~<p>We will paste here some parts of the safety form of our team.</p>~~

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~~<p>We also discussed safety of our team in <a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">human practice page</a>.</p>~~

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~~<h3>Your Training</h3>~~

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~~<p>a) Have your team members received any safety training yet?</p>~~

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~~<p>We have not had any safety training officially, but have been taught by learned people.</p>~~

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~~<p>b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.</p>~~

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<p>We learned about techniques for preventing diffusion of Escherichia coli or other organisms including ogenetically modified organisms into environment and risks concerning DNA assembly experiments.</p>

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<p>c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.</p>

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<p>Division for Environment, Health and Safety (http://www.adm.u-tokyo.ac.jp/office/anzeneisei/index.html) in our university is responsible for training laboratory safety. <br />Taking lab safety training course is not mandatory, but strongly recommended for those who are involved in experiments. However, this course is for the graduate school students and senior stuffs, and not open to undergraduate students. We thus had a training directly from the PI.</p>

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~~<h3>The Organisms and Parts that You Use</h3>~~

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<tr><th>Species name(including strain)</th><th>Risk Group</th><th>Risk Group Source</td><th>Disease risk to humans?</td><th>Part number/name</th><th>Natural function of part</th><th>How did you acquire it?</th><th>How will you use it?</th><th>Notes</th></tr>

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~~<tr><td>Escherichia coli JM109</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>from our Lab</td><td>DNA asssembly</td><td></td></tr>~~

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~~<tr><td>Escherichia coli MG1655</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>from our Lab</td><td>Assay</td><td></td></tr>~~

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<tr><td>Pseudomonas fluorescens</td><td>2</td><td>http://www.absa.org/riskgroups/bacteriasearch.php?genus=Pseudomonas</td><td>yes</td><td>sigma factor</td><td>polymerize RNA with RNA polymerase</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td>The bacteria causes opportunistic infection and it affects with usually patients with compromised immune systems.</td></tr>

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<tr><td>Pseudomonas protegens</td><td>1</td><td>http://www.dsmz.de/catalogues/details/culture/DSM-19095.html</td><td>no</td><td>anti sigma factor</td><td>prevent combination between RNA polymerase and specific sigma factor</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td></td></tr>

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<tr><td>Pseudomonas syringae</td><td>1</td><td>DSMZ</td><td>no</td><td>sigma factor</td><td>polymerize RNA with RNA polymerase</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td></td></tr>

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<tr><td>Pseudomonas syringae</td><td>1</td><td>DSMZ</td><td>no</td><td>anti sigma factor</td><td>prevent combination between RNA polymerase and specific sigma factor</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td></td></tr>

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~~<tr><td>Chlorocebus aethiops COS-1</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>receive the cells from another lab</td><td>Assay</td><td></td></tr>~~

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~~<tr><td>Chlorocebus aethiops COS-7</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>receive the cells from another lab</td><td>Assay</td><td></td></tr>~~

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~~<tr><td>Homo sapiens HL-60</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>receive the cells from another lab</td><td>Assay</td><td></td></tr>~~

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<tr><td>Homo sapiens oral epithelial cell</td><td>1</td><td>http://www.lifescience.mext.go.jp/bioethics/data/anzen/syourei_02.pdf</td><td>no</td><td>EGP2 promoter</td><td>the promoter of EpCAM</td><td>from the member of our team</td><td>transcriptional control</td><td></td>

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~~<h3>Risks of Your Project Now</h3>~~

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<p>Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.</p>

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~~<p>a) Risks to the safety and health of team members, or other people working in the lab</p>~~

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<p>When we are exposed to E. coli cells, we have a chance to have irritation in our eyes, skin, and respiratory system. Furthermore, ethidium bromide which we use to detect DNA band is carcinogen. We also use mammalian cells in assay. These cells can be infected by viruses which can also infect us. We also use harmful reagent, such as membrane binding solution.</p>

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~~<p>b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):</p>~~

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~~<p>As maintained above, our lab has harmful organisms and substances. If they diffused outside, there might be health hazard.</p>~~

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~~<p>c) Risks to the environment (from waste disposal, or from materials escaping from your lab)</p>~~

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<p>We might dispose chips or tubes which contain a bit amount of genetically modified organisms without sterilizing. It offenses the law of our country; they may effect on biodiversity in our country. The bacteria we used also have drug resistance against ampicillin or CP, and if escaped, they could not be killed by those drugs. Mammalian cells are so weak that they can hardly live in the natural world.</p>

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~~<p>d) Risks to security through malicious mis-use by individuals, groups, or countries</p>~~

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~~<p>There seems to be no risks with this subject. In today's cognition, parts that we use do not seem to lead the expression of harmful materials.</p>~~

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~~<p>e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</p>~~

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<p>In order to avoid the risks about bacterial cells shown above, we autoclave all wastes, sterilize all equipments that contain the organisms with detergent or hypochlorous acid. When bacteria exposed outside the tubes or examiners, we immediately seterilize them with ehtanol. Furthermore, in order to keep ethidium bromide away from our skin, we use kimwipes to wrap the chips used to taking it up before throwing away, and we take care that our bare hands do not touch the gel polluted by the substance.</p>

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~~<h3>Risks of Your Project in the Future</h3>~~

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<p>What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.</p>

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<p>a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</p>

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<p>In sigma-Recounter project, the circuit we constructed has potential for the application for defeating pests or bacteria by means of the expression of toxic proteins. In this case, there is a risk that you mistakenly output the toxin.<br />In CTCD project, the role of the circuits is to detect CTCs by GFP, thus it is thought to be difficult to have a risk of doing humans or environment harm.</p>

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<p>b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.</p>

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<p>In the future study of sigma-Recounter project, in addition to the reset system, we intend to increase the number of nodes and to enable one state to move to any other states. Therefore, if our project is used to express a toxin to defeat pest or bacteria, you can prepare an anti-toxin node or a reset system for mistakenly expressing the toxin.</p>

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~~<div id = "Attribution-1">~~

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~~<img src="https://static.igem.org/mediawiki/2014/4/44/Sub_attribution.png" class = "contTitle" />~~

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<p>Our activities involved in iGEM were all conducted by undergaduates alone!!<br>Based on fund-raisings and public relations, all team members had a lot of brainstoming, investigations and discussions in order to select project carefully. And we conducted experiments and FINALLY saw results.<br>We also designed and composed all publish tools, and of course, polished all scripts and presentation by ourselves.</p>

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~~<h3>Project</h3>~~

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<p>All we had a lot of brainstorming, investigations and discussion when we decide our projects.<br>σ-ReCounter:<br><b>Shunsuke Sumi</b>(idea)<br><b>So Nakashima</b>(idea and comformation)<br><b>Takefumi Yoshikawa</b>(comformation)<br><br>CTCD:<br><b>Masayuki Osawa</b>(conformation)<br><b>Shigetaka Kobari</b>(investigation and conformation)<br><b>Shunsuke Sumi</b>(conformation)<br><b>Senkei Hyo</b>(investigation)<br><b>Yoshihiko Tomofuji</b>(conformation)<br><b>Yshiki Okesaku</b>(investigation)</p>

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~~<h3>Experiment</h3>~~

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<p>Lab. Leader:<br><b>Takefumi Yoshikawa</b>(construction, assay;σ-ReCounter)<br><br>Lab. Members:<br><b>Atsuki Ito</b>(construction)<br><b>Hajime Takemura</b>(construction)<br><b>Keisuke Tsukada</b>(construction)<br><b>Kentaro Tara</b>(construction)<br><b>Kento Nakamura</b>(construction, assay;σ-ReCounter)<br><b>Naruki Yoshikawa</b>(construction)<br><b>Nobuhiro Hiura</b>(construction)<br><b>Shigetaka Kobari</b>(assay;CTCD)<br><b>So Nakashima</b>(construction, Assay;σ-ReCounter)<br><b>Yoshihiko Tomofuji</b>(assay;CTCD)<br><b>Yuto Yamanaka</b>(construction)</p>

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~~<h3>Modeling</h3>~~

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~~<p><b>Keisuke Tsukada, Kentaro Tara, Manabu Nishiura, Masaki Ono</b></p>~~

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~~<h3>Web</h3>~~

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~~<p>Almost all members wrote drafts of our team wiki.<br><b>Cristian David</b>(check our English)<br><b>Hiroki Tsuboi</b>(team website, implementation of our team wiki)</p>~~

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~~<h3>App</h3>~~

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~~<p><b>Naruki Yoshikawa</b></p>~~

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~~<h3>Design</h3>~~

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~~<p><b>Cristian David</b>(parker design)<br><b>Yoshiki Okesaku</b>(all design, all figure)</p>~~

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~~<h3>Presentation</h3>~~

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~~<p><b>Kento Nakamura, Manabu Nishiura, Masato Ishikawa, Yumeno Koga, Yuto Yamanaka</b></p>~~

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~~<h3>Poster</h3>~~

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~~<br>~~

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~~<h3>Public Relation</h3>~~

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~~<p><b>Ding Yuewen, Keisuke Tsukada</b></p>~~

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~~<h3>Adviser</h3>~~

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~~<p><b>Kota Tosimitsu</b></p>~~

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~~</div>~~

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~~<div id = "Attribution-2">~~

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~~<img src="https://static.igem.org/mediawiki/2014/4/44/Sub_Ackno.png" class="contTitle" />~~

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~~</div>~~

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~~<div id = "Attribution-3">~~

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~~<img src="https://static.igem.org/mediawiki/2014/3/38/Sub_sponsors.png" class="contTitle" />~~

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~~<img src="https://static.igem.org/mediawiki/2014/7/72/Promega.png" class="sponsor">~~

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~~<p><b>Promega KK.</b>for chamical reagents</p>~~

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~~<p><b>Teiyukai, Faculty of Engineering, The University of Tokyo</b>for fund</p>~~

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~~<p><b>Integrated DNA Technologies MBL</b></p>~~

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~~<img src="https://static.igem.org/mediawiki/2014/3/30/Cosmobio.png" class="sponsor">~~

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~~<p><b>COSMO BIO Co., Ltd.</b>for fund<br></p>~~

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~~<img src="https://static.igem.org/mediawiki/2014/1/17/Liveanest.png" class="sponsor">~~

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~~<p><b>Leave a Nest Co., Ltd.(Hiroyuki Takahashi)</b>for advice for public relations & introduction of Promega KK.</p>~~

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~~</div>~~

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~~<div id = "Humanpractice-1">~~

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~~<img src = "https://static.igem.org/mediawiki/2014/1/1c/Sub_introduction.png" class = "contTitle"/>~~

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~~<p>This year, we set 2 goals in human practice and have made efforts to realize following goals.</p>~~

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~~<p><b>1.Spreading iGEM in general public.</b></p>~~

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~~<p><b>2.Activating iGEM in Japan.</b></p>~~

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<p>We set these 2 goals because we want to remove prejudice against gene recombination and synthetic biology, and familiarize people with synthetic biology. By doing these, we think that people can understand synthetic biology better.</p>

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~~<p>We also expect undergraduate students, who will lead next generation of biology, to improve their skills by activating iGEM in Japan.</p> </div>~~

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~~<div id = "Humanpractice-2">~~

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~~<img src="https://static.igem.org/mediawiki/2014/4/4b/Sub_to_spread_igem_in_general_public.png" class = "contTitle" />~~

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~~<h3>"EcoLightsOut"</h3>~~

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<p>We have developed an Android app. to introduce our project. This is a puzzle game which uses the counting system we created. In the project, the state of E. coli is changed by stimulus of signal molecules. In the game, players can stimulate the E. coli by touching it. E. coli are displayed in 3x3 or 5x5 grid shape. When you touch an E. coli in the game, the colors of the E. coli and four adjacent ones are changed. This behavior is analogy of diffusion of signal molecules. The goal is to turn all E. coli green.</p>

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<p>Synthetic biology is not well-known and thought to be unfamiliar to ordinary people. To introduce synthetic biology into public, an easy entrance such as playing game is effective. We hope that players will get interested in synthetic biology by enjoying this game.</p>

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~~<p>You can download this game from Google Play.</p>~~

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~~<h3>Lectures to general public</h3>~~

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~~<h4>School festivals</h4>~~

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<p>The university of Tokyo has two school festivals per year. The May festival is held in May and the Komaba festival was held in November.We explained iGEM and synthetic biology briefly and introduce our project to audience. We　invited other iGEM teams in Japan to May festival. We offered precious opportunities that Japanese iGEM teams meet through May festivals.</p>

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~~<h4>Techno-Edge</h4>~~

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<p>Techno-Edge is the event which the department of technology of university of Tokyo held. The purpose of this event was to appeal department of technology to junior high or high school students.Many laboratories and academic circles such as Robotech took part in this event. iGEM UT-Tokyo also participated in it to appeal synthetic biology.</p>

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~~<p>Not only high school and junior high, but also primary school students came to our booth and were interested in our explanation.</p>~~

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~~<h4>Presentation</h4>~~

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<p>This year, iGEM Nagahama invited us to the genetics society of Japan, and we participated in it. We made an oral presentation workshop of synthetic biology and took part in poster session. There were many iGEM teams, and we could advertise iGEM in academic world. Moreover, specialists gave advice to us, and we were inspired by professors of synthetic biology.</p>

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~~<p>We also joined in Japanese Society for Cell Synthesis Research.</p>~~

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~~<h4>A cram school</h4>~~

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~~<p>We held a seminar in which we explained synthetic biology and iGEM for high school students at a cram school in Komaba.</p>~~

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~~</div>~~

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~~<div id = "Humanpractice-3">~~

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~~<img src="https://static.igem.org/mediawiki/2014/3/34/Sub_to_activate_igem.png" class = "contTitle" />~~

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~~<h3>Collaborations</h3>~~

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~~<p>We collaborated on modeling with Nagahama.</p>~~

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~~<p>Their project aimed cadmium collection using <i>Escherichia coli</i> which has positive chemotaxis for aspartic acid, then we constructed simplified model of chemotaxis~~

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~~and simulated behavior of <i>E. coli</i> by using probability and random function.</p>~~

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<p>Their wiki explained the result of modeling.(→link) We constructed all equations in their simulation including the equation that determines <i>E. coli</i> to choose going straight or turning in probability, and explained equations to them.The figure describing the result of simulation is also made by UT-Tokyo. All code for modeling is here.(→link)</p>

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~~<h3>Ethics and regulation</h3>~~

-

~~<p>We thought to confirm whether our project meets ethics, but ethics is very vague, so we decided to research about ethics.</p>~~

-

~~<h3>iGEM Japan</h3>~~

-

~~<p>iGEM Japan is an organization which was founded last year for iGEM teams in Japan to cooperate each other.</p>~~

-

<p>In March, iGEM Kyoto held iGEM-Japan West meeting. In this meeting, we shared each team's project and advised each other. This meeting was very useful because we could find our idea's weak point.</p>

-

~~<p>In August, iGEM TMU-Tokyo held iGEM-Japan East meeting, and we made presentations about our projects and criticized each other. Thanks to these meetings, we developed quality of our projects.</p>~~

-

~~</div>~~

</div>

Line 273:

Line 103:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">YOICHI IRIE</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Yoichi Irie</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo, ~~Literature, Sophmore~~</dd>

+

<dd>The University of Tokyo, Arts and Sciences</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Team leader</dd>

</dl>

-

<p class ="comment">~~I want~~ to ~~be a doctor.~~</p>

+

<p class ="comment">My dream is to make me robuster against stress caused by iGEM in synthetic biology!</p>

</div>

Line 297:

Line 127:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name" style="font-size:35px;">CRISTIAN DAVID PENA MARINEZ</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Cristian David Pena Martinez</dd>

<dt>Belong to</dt>

-

<dd>~~The University~~ of ~~Tokyo~~, ~~Literature~~, ~~Sophmore~~</dd>

+

<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Design</dd>

</dl>

-

<p class ="comment">~~I want to be a doctor.~~</p>

+

<p class ="comment">"The only concrete proof of the human existence is: poetry" - Luis Cardoza y Aragon</p>

</div>

Line 327:

Line 157:

<dd>Masaki Ono</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo, ~~Literature, Sophmore</dd>~~

+

<dd>The University of Tokyo, Arts and Sciences, Sophmore</dd>

-

~~<dt>Job</dt>~~

+

-

~~<dd>Modeling</dd>~~

+

-

~~</dl>~~

+

-

~~<p class ="comment">I want to be a doctor.</p>~~

+

-

~~</div>~~

+

-

~~</div>~~

+

-

~~</div>~~

+

-

~~<div class = "element-item">~~

+

-

~~<div class = "thumbnail">~~

+

-

~~<img src = "https://static.igem.org/mediawiki/2014/a/a1/Ishikawa.png" class ="member-thumbnail" />~~

+

-

~~</div>~~

+

-

~~<div class = "detail">~~

+

-

~~<div class = "meta">~~

+

-

~~<p>iGEM UT-Tokyo</p>~~

+

-

~~<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />~~

+

-

~~</div>~~

+

-

~~<div class = "member">~~

+

-

~~<p class = "name">MASAKI ONO</p>~~

+

-

~~<img src = "https://static.igem.org/mediawiki/2014/7/78/Cocoa_comb.png" class ="member-detail" />~~

+

-

~~<dl class = "profile">~~

+

-

~~<dt>Name</dt>~~

+

-

~~<dd>Masaki Ono</dd>~~

+

-

~~<dt>Belong to</dt>~~

+

-

~~<dd>The University of Tokyo, Literature~~, Sophmore</dd>

+

<dd>Modeling</dd>

Line 369:

Line 175:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">YUMENO KOGA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Yumeno Koga</dd>

<dt>Belong to</dt>

-

<dd>~~The University~~ of ~~Tokyo~~, ~~Literature~~, ~~Sophmore~~</dd>

+

<dd>Department of Lifescience & Medical Bioscience, School of Advanced Science and Engineering, Waseda University</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Presenter</dd>

</dl>

-

<p class ="comment">I ~~want to be~~ a ~~doctor~~.</p>

+

<p class ="comment">I'm a "wasejo".</p>

</div>

Line 393:

Line 199:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">KENTO NAKAMURA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Kento Nakamura</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

+

<dd>College of Arts and Sciences, The University of Tokyo</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Experimenter, Presenter</dd>

</dl>

-

<p class ="comment">I ~~want to be a doctor~~.</p>

+

<p class ="comment">"I think, therefore I am" by R. Descartes<br />"I multiply, therefore I am" by E.coli <br />"I hope so..." by Experimenter</p>

</div>

Line 417:

Line 223:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">SOH NAKASHIMA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Soh Nakashima</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

+

<dd>College of Arts and Sciences, The University of Tokyo</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Experimenter</dd>

</dl>

-

<p class ="comment">~~I want to be~~ a ~~doctor~~.</p>

+

<p class ="comment">follow me on Twitter(<a href="https://twitter.com/dolicas_" target="_blank" >@dolicas_</a>)</p>

</div>

Line 441:

Line 247:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">MANABU NISHIURA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Manabu Nishiura</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo, ~~Literature, Sophmore~~</dd>

+

<dd>The University of Tokyo, Arts and Sciences</dd>

-

<dd>Modeling</dd>

+

<dd>Modeling,Presenter</dd>

</dl>

-

<p class ="comment">I ~~want to be~~ a ~~doctor~~.</p>

+

<p class ="comment"> I am a Feynman Diagram.</p>

</div>

Line 465:

Line 271:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">YOSHIKI OKESAKU</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Yoshiki Okesaku</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

+

<dd> Department of Physics, Graduate School of Science, The University of Tokyo</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Design(DOKATA)</dd>

</dl>

-

<p class ="comment">I ~~want to be a doctor~~.</p>

+

<p class ="comment">I am majoring in the MDS theory. The MDS theory can describe how our universe had begun and will end.</p>

</div>

Line 489:

Line 295:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">HAJIME TAKEMURA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Hajime Takemura</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo, ~~Literature~~, ~~Sophmore~~</dd>

+

<dd>The University of Tokyo, Junior,Faculty of Pharmaceutical Science</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Experiment</dd>

</dl>

-

<p class ="comment">I want to be a ~~doctor~~.</p>

+

<p class ="comment">I want to make a new medicine.</p>

</div>

Line 513:

Line 319:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">KENTARO TARA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Kentaro Tara</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

+

<dd>Arts and Sciences, The University of Tokyo</dd>

-

<dd>Modeling</dd>

+

<dd>Experiment, Modeling</dd>

</dl>

-

<p class ="comment">~~I want to be a doctor~~.</p>

+

<p class ="comment">Maps relax me.</p>

</div>

Line 537:

Line 343:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">YUEWEN DING</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Yuewen Ding</dd>

<dt>Belong to</dt>

-

<dd>The ~~University~~ of Tokyo~~, Literature, Sophmore~~</dd>

+

<dd>Faculty of Pharmaceutical Science, The university of Tokyo</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Public relation</dd>

</dl>

-

<p class ="comment">~~I want to be a doctor~~.</p>

+

<p class ="comment">Eating is sweeter than Life.</p>

</div>

Line 561:

Line 367:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">YOSHIHIKO TOMOFUJI</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Yoshihiko Tomofuji</dd>

<dt>Belong to</dt>

-

<dd>The ~~University~~ of Tokyo, ~~Literature, Sophmore~~</dd>

+

<dd>The university of Tokyo, faculty of medicine</dd>

-

<dd>~~Modeling~~</dd>

+

</dl>

-

<p class ="comment">I ~~want to be~~ a ~~doctor~~.</p>

+

</div>

Line 585:

Line 391:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">KOHTA TOSHIMITSU</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Kohta Toshimitsu</dd>

<dt>Belong to</dt>

-

<dd>~~The University~~ of ~~Tokyo~~, ~~Literature, Sophmore~~</dd>

+

<dd>Faculty of Pharmacy,　Keio University</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Adviser</dd>

</dl>

-

<p class ="comment">I ~~want to be a doctor~~.</p>

+

<p class ="comment">I'm so sick of pipetting.</p>

</div>

Line 609:

Line 415:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">KEISUKE TSUKADA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Keisuke Tsukada</dd>

<dt>Belong to</dt>

-

<dd>The University of Tokyo~~, Literature, Sophmore~~</dd>

+

<dd>Department of Biophysics and Biochemistry , Faculty of Science, The University of Tokyo</dd>

-

<dd>Modeling</dd>

+

<dd>Experiment, Modeling</dd>

</dl>

-

<p class ="comment">~~I want to be a doctor.~~</p>

+

<p class ="comment">itininnmaenokenkyuusyaninaretaraiina</p>

</div>

Line 633:

Line 439:

</div>

-

<p class = "name">~~MASAKI ONO~~</p>

+

<p class = "name">YUTO YAMANAKA</p>

-

<dd>~~Masaki Ono~~</dd>

+

<dd>Yuto Yamanaka</dd>

<dt>Belong to</dt>

-

<dd>~~The University~~ of ~~Tokyo~~, ~~Literature~~, ~~Sophmore~~</dd>

+

<dd>Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University</dd>

-

<dd>~~Modeling~~</dd>

+

<dd>Experimenter, Presenter</dd>

+

</dl>

+

<p class ="comment">I like Falcon tubes.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">SHUNSUKE SUMI</p>

+

+

+

+

<dd>Shunsuke Sumi</dd>

+

<dt>Belong to</dt>

+

<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>

+

+

+

</dl>

+

<p class ="comment">a tree has no emotion.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">NARUKI YOSHIKAWA</p>

+

+

+

+

<dd>Naruki Yoshikawa</dd>

+

<dt>Belong to</dt>

+

<dd>College of Arts and Sciences, The University of Tokyo</dd>

+

+

<dd>Experimenter</dd>

+

</dl>

+

<p class ="comment">Three billion devices run Java, but my devices don't.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">NOBUHIRO HIURA</p>

+

+

+

+

<dd>Nobuhiro Hiura</dd>

+

<dt>Belong to</dt>

+

<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>

+

+

<dd>Experimenter</dd>

+

</dl>

+

<p class ="comment">Let's go to Saitama, Japan!</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">HIROKI TSUBOI</p>

+

+

+

+

<dd>Hiroki Tsuboi</dd>

+

<dt>Belong to</dt>

+

<dd>Waseda University, School of Fundamental Science and Engineering, Computer Science and Engineering</dd>

+

+

+

</dl>

+

<p class ="comment">After wiki freeze, I will make other Web Site</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">SHIGETAKA KOBARI</p>

+

+

+

+

<dd>Shigetaka Kobari</dd>

+

<dt>Belong to</dt>

+

<dd>The university of Tokyo, faculty of medicine</dd>

+

+

+

</dl>

+

<p class ="comment">a tree has no emotion.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">MASATO ISHIKAWA</p>

+

+

+

+

<dd>Masato Ishikawa</dd>

+

<dt>Belong to</dt>

+

<dd>College of Arts and Sciences, The University of Tokyo</dd>

+

+

<dd>Presenter</dd>

+

</dl>

+

<p class ="comment">I want to study pharmacy.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">MASAYUKI OSAWA</p>

+

+

+

+

<dd>Masayuki Osawa</dd>

+

<dt>Belong to</dt>

+

<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>

+

+

<dd>Woodkeeper</dd>

+

</dl>

+

<p class ="comment">I take care of crazy tree.</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">TAKEHUMI YOSHIKAWA</p>

+

+

+

+

<dd>Takehumi Yoshikawa</dd>

+

<dt>Belong to</dt>

+

<dd>Department of Bioinformatics and Systems Biology, Faculty of Science, The University of Tokyo</dd>

+

+

<dd>Experiment Leader</dd>

+

</dl>

+

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">ATSUKI ITO</p>

+

+

+

+

<dd>Atsuki Ito</dd>

+

<dt>Belong to</dt>

+

<dd>Department of Bioscience, Faculty of Engineering,Tokyo University of Science</dd>

+

+

<dd>Experimenter</dd>

+

</dl>

+

<p class ="comment">kimuwaipu,azinai...</p>

+

</div>

+

</div>

+

</div>

+

+

+

+

</div>

+

+

+

<p>iGEM UT-Tokyo</p>

+

+

</div>

+

+

<p class = "name">PROF.WAKAMOTO</p>

+

+

+

+

<dd>Yuichi Wakamoto</dd>

+

<dt>Belong to</dt>

+

<dd>Center for Complex Systems Biology The University of Tokyo, Dapartment of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo</dd>

+

+

<dd>Instructor</dd>

</dl>

-

~~<p class ="comment">I want to be a doctor.</p>~~

</div>

Line 649:

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</div>

-

~~~~

</div>

-

~~~~

</div>

@@ Line 15: / Line 15: @@
 <li id="Result"><a href="Javascript:open('Result')"><img src="https://static.igem.org/mediawiki/2014/1/11/LinkCTC_resulton.png" class="contList" alt="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_resultopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Result-block','Result-1')">miRNA-binding sites</a></li>
-<li><a href="#">Contents2</a></li>
 </ul>
 </li>
 <li id="Lab"><a href="Javascript:open('Lab')"><img src="https://static.igem.org/mediawiki/2014/6/6d/LinkCTC_labon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/d/de/LinkCTC_labopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Lab-block','Lab-1')">Parts</a></li>
-<li><a href="#">Contents2</a></li>
+<li><a href="Javascript:loadContent('Lab-block','Lab-2')">Protocol</a></li>
+<li><a href="Javascript:loadContent('Lab-block','Lab-3')">Lab Note</a></li>
 </ul>
 </li>
 <li id="Modeling"><a href="Javascript:open('Modeling')"><img src="https://static.igem.org/mediawiki/2014/4/42/LinkCTC_modelingon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/5/56/LinkCTC_modelingopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Modeling-block','Modeling-1')">BBa_K747096</a></li>
-<li><a href="#">Contents2</a></li>
 </ul>
 </li>
-<li id="Achieve"><a href="Javascript:open('Achieve')"><img src="https://static.igem.org/mediawiki/2014/8/88/LinkCTC_achieon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/4/41/LinkCTC_achieopen.png"></a>
+<li id="Achievement"><a href="Javascript:open('Achievement')"><img src="https://static.igem.org/mediawiki/2014/8/88/LinkCTC_achieon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/4/41/LinkCTC_achieopen.png"></a>
 <ul class="subContList">
-<li><a href="#">Contents1</a></li>
+<li><a href="Javascript:loadContent('Achievement-block','Achievement-1')">Judging Form</a></li>
-<li><a href="#">Contents2</a></li>
 </ul>
 </li>
-<li id="Safety"><a href="Javascript:open('Safety')"><img src="https://static.igem.org/mediawiki/2014/9/96/LinkCTC_safetyon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/93/LinkCTC_safetyopen.png"></a>
+<li id="Safety"><a href="Javascript:loadContent('Safety-block','top')"><img src="https://static.igem.org/mediawiki/2014/9/96/LinkCTC_safetyon.png" class="contList"></a>
-<ul class="subContList">
-<li><a href="#">Contents1</a></li>
-<li><a href="#">Contents2</a></li>
-</ul>
 </li>
 <li id="Attribution"><a href="Javascript:open('Attribution')"><img src="https://static.igem.org/mediawiki/2014/3/38/LinkCTC_attributionon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/9/92/LinkCTC_attributionopen.png"></a>
@@ Line 50: / Line 44: @@
 </ul>
 </li>
-<li id="Humanpractice"><a href="Javascript:open('Humanpractice')"><img src="https://static.igem.org/mediawiki/2014/3/39/Link_humanpracticeclose.png" class="contList" alt="https://static.igem.org/mediawiki/2014/0/07/Link_humanpracticeopen.png" /></a>
+<li id="Humanpractice"><a href="Javascript:open('Humanpractice')"><img src="https://static.igem.org/mediawiki/2014/5/5e/LinkCTC_humanon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/c/c9/LinkCTC_humanopen.png"></a>
 <ul class="subContList">
-<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">Introduction</a></li>
+<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-1')">Human Practice</a></li>
-<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">To spread iGEM in general public</a></li>
+<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">Ethics</a></li>
-<li><a href="Javascript:loadContent('Humanpractice-block','Humanpractice-3')">To activate iGEM</a></li>
 </ul>
 </li>
-<li id="Team"><a href="Javascript:loadContent('Team-top')"><img src="https://static.igem.org/mediawiki/2014/8/83/LinkCTC_teamon.png" class="contList" alt="https://static.igem.org/mediawiki/2014/3/31/LinkCTC_teamopen.png"></a>
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 			<!-- Projectここから -->
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-				<!-- Project Introduction -->
-				<div id = "Project-1">
-					<img src="https://static.igem.org/mediawiki/2014/d/d9/SubCTC_introduction.png" class = "contTitle" />
-					<p>Please imagine that you are a police officer. You are searching for dangerous criminals who hide in crowd and it is difficult to find them. This situation is the same as finding cancer cells (dangerous criminals in our body) in our blood when they resemble to normal blood cells. In this way picking up cancer cells has been developed around the world, where every year a different method is developed. Don't you think that it is like a dream if there were drugs, which if someone take, they will confess their crime? What about drugs, which if the cancer cells take, they will shine brightly on the blood? Here our team focuses on microRNA (miRNA) and provides a special genetic circuit which exposes cancer cells in blood vessels.(Fig.1)</p>
-					<img src="https://static.igem.org/mediawiki/2014/f/fa/Introduction_CTC_fig1.png" class ="figure" />
-					<p>Circulating tumor cells (CTCs) tumor cells arising from epithelia, flowing through blood vessels and causing metastasis to other tissues. Focusing on these cells, there are ways of early detection of cancer, detecting CTCs in blood of patients with early-stage cancer. The effectiveness of these methods has been ensured through the results of researches especially in breast cancer and colon cancer (ref). These methods of detection are a technology developed in recent years, compared to the traditional methods of picking up the tissue of patients which has been used for a long time. However, these traditional methods produce a hard burden to the patients, because of the several and long procedures. On the other hand, the methods of detecting CTCs produce a non-invasive option for patients compared to traditional ones. From the utility points, CTCs have been used all around the world to develop several detection methods. Most of these methods emphasized utilizing anti-EpCAM antibody, which is an adhesion protein used as marker of cancers, and distinguish CTCs from blood cells by the difference of their cell size. However, because the concentration of CTCs in blood is very low, it is required to develop a new approach for the detection of CTCs.</p>
-					<p>Here we have developed a new detection method for CTCs focusing on two types of profiles; miRNA and EpCAM. It has given the more specificity for CTCs to utilize the two profiles. miRNA is a special short non-cording RNA and interferes the translation of the complementary mRNA, interacting with some proteins and forming the RNA-induced silencing complex (RISC) in wide-species from virus to human. Focusing on different expression patterns of miRNA in each cell type, we utilized miRNA profiles with a synthetic biological approach for detecting CTCs. The circuit that we have developed has EpCAM promoter, reporter and blood cells-specific miRNA (<i>miR-142-3p/5p</i>) binding sites. Two conditions, which are activation of EpCAM promoter and an absence of blood cells-specific miRNA, are required for translation of the reporter. If we inject this circuit into cells in blood, only in the case of CTCs the reporter is produced.(Fig.2)</p>
-					<img src="https://static.igem.org/mediawiki/2014/1/13/Introduction_CTC_fig2.png" class ="figure" />
-					<p>[1] Friedlander, Terence W., Gayatri Premasekharan, and Pamela L. Paris. "Looking back, to the future of circulating tumor cells." <i>Pharmacology & therapeutics</i> 142.3 (2014): 271-280.</p>
-				</div>
-				<!-- Project system -->
-				<div id = "Project-2">
-				<img src = "https://static.igem.org/mediawiki/2014/8/8f/SubCTC_system.png" class = "contTitle"/>
-				<img src="https://static.igem.org/mediawiki/2014/6/64/Kobari_pegp2.png" class = "figure" />
-				<legend style="margin-left:300px;"><b>Fig.3</b> the construct of our circuit</legend>
-				<h3>EGP-2 promoter</h3>
-				<p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.EpCAM is a trans membrane glycoprotein which is expressed in epithelial cells and shows high level expression in various type of human epithelial carcinomas[2].Using this promoter, reporter genes can be expressed only in cancer cells. EGP-2 promoter is originally about 3500bp and it is not convenient to make a DNA construct. But, in the previous research, it is known that EGP-2 promoter can work  if a part of its sequence is deleted[3]. In this project ,We cloned a part of EGP-2 promoter, and made it useful as a biobrick parts.</p>
-				<h3>miRNA-142</h3>
-				<p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[4].After miRNA-142 is transcribed, it is cleaved to miRNA-142-5p and miRNA-142-3p.  Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.</p>
-				<img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" />
-				<legend><b>Fig.4</b> When this construct is transfected to the epithelial cells like cancer cell, pEGP-2 promote the transcription of EGFP, and the mRNA is not degraded by miRNA because miR 142 is specific to hematopoietic cells.</legend>
-				<img src="https://static.igem.org/mediawiki/2014/4/44/Kobari_hematopoietic_cell.png" class = "figure" />
-				<legend><b>Fig.5</b> When this construct is transfected to the hematopoietic cells, pEGP-2 does not work as a promoter, and mRNA is not transcribed. Even if the leak of the promoter makes few mRNA ,this mRNA is degraded by RNAi caused by miR 142.</legend>
-				</div>
-				<!-- Project application -->
-				<div id = "Project-3">
-				<img src = "https://static.igem.org/mediawiki/2014/b/b4/SubCTC_application.png" class = "contTitle" />
-				<p>In our project, miRNA is used for degrading mRNA in cells that express miRNA. But if we transfect construct like [pCMV-LacI-miR A binding site] and [pCAG-LacO2-GFP][5], mRNA in cells that does not express miRNA is degraded. This system will makes many application of miRNA in iGEM possible.</p>
-				<p>We focused on a tissue-specific promoter and miRNA in order to detect CTCs.As well as hematopoietic cells, many other tissues shows specific miRNA expression patterns[3]. Therefore, if by making a circuit that returns an output to a certain miRNA expression pattern, we may identify where a CTC comes from.[5]</p>
-				<p>In addition to the detection of CTCs, combinations of tissue-specific promoter and miRNA can be applied to many treatments. For example, suicide genes can be expressed in cancer cells in a specific tissue.[6]</p>
-				</div>
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 			<!-- Safety -->
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-					<p>We will paste here some parts of the safety form of our team.</p>
-					<p>We also discussed safety of our team in <a href="Javascript:loadContent('Humanpractice-block','Humanpractice-2')">human practice page</a>.</p>
-					<h3>Your Training</h3>
-					<p>a) Have your team members received any safety training yet?</p>
-					<p>We have not had any safety training officially, but have been taught by learned people.</p>
-					<p>b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.</p>
-					<p>We learned about techniques for preventing diffusion of Escherichia coli or other organisms including ogenetically modified organisms into environment and risks concerning DNA assembly experiments.</p>
-					<p>c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.</p>
-					<p>Division for Environment, Health and Safety (http://www.adm.u-tokyo.ac.jp/office/anzeneisei/index.html) in our university is responsible for training laboratory safety. <br />Taking lab safety training course is not mandatory, but strongly recommended for those who are involved in experiments. However, this course is for the graduate school students and senior stuffs, and not open to undergraduate students. We thus had a training directly from the PI.</p>
-					<h3>The Organisms and Parts that You Use</h3>
-					<table cellspacing="0" cellpadding="0" style="background-color:#FFF;width:890px; word-break:break-word;margin-left:10px;text-align:center" border="1">
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-					  <tr><th>Species name(including strain)</th><th>Risk Group</th><th>Risk Group Source</td><th>Disease risk to humans?</td><th>Part number/name</th><th>Natural function of part</th><th>How did you acquire it?</th><th>How will you use it?</th><th>Notes</th></tr>
-					  <tr><td>Escherichia coli JM109</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>from our Lab</td><td>DNA asssembly</td><td></td></tr>
-					  <tr><td>Escherichia coli MG1655</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>from our Lab</td><td>Assay</td><td></td></tr>
-					  <tr><td>Pseudomonas fluorescens</td><td>2</td><td>http://www.absa.org/riskgroups/bacteriasearch.php?genus=Pseudomonas</td><td>yes</td><td>sigma factor</td><td>polymerize RNA with RNA polymerase</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td>The bacteria causes opportunistic infection and it affects with usually    patients with compromised immune systems.</td></tr>
-					  <tr><td>Pseudomonas protegens</td><td>1</td><td>http://www.dsmz.de/catalogues/details/culture/DSM-19095.html</td><td>no</td><td>anti sigma factor</td><td>prevent combination between RNA polymerase and specific sigma factor</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td></td></tr>
-					  <tr><td>Pseudomonas syringae</td><td>1</td><td>DSMZ</td><td>no</td><td>sigma factor</td><td>polymerize RNA with RNA polymerase</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td></td></tr>
-					  <tr><td>Pseudomonas syringae</td><td>1</td><td>DSMZ</td><td>no</td><td>anti sigma factor</td><td>prevent combination between RNA polymerase and specific sigma factor</td><td>order the part DNA from a synthesis company</td><td>transcriptional control</td><td></td></tr>
-					  <tr><td>Chlorocebus aethiops COS-1</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>receive the cells from another lab</td><td>Assay</td><td></td></tr>
-					  <tr><td>Chlorocebus aethiops COS-7</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>receive the cells from another lab</td><td>Assay</td><td></td></tr>
-					  <tr><td>Homo sapiens HL-60</td><td>1</td><td>DSMZ</td><td>no</td><td></td><td></td><td>receive the cells from another lab</td><td>Assay</td><td></td></tr>
-					  <tr><td>Homo sapiens oral epithelial cell</td><td>1</td><td>http://www.lifescience.mext.go.jp/bioethics/data/anzen/syourei_02.pdf</td><td>no</td><td>EGP2 promoter</td><td>the promoter of EpCAM</td><td>from the member of our team</td><td>transcriptional control</td><td></td>
-					  </tr>
-					</table>
-					<h3>Risks of Your Project Now</h3>
-					<p>Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.</p>
-					<p>a) Risks to the safety and health of team members, or other people working in the lab</p>
-					<p>When we are exposed to E. coli cells, we have a chance to have irritation in our eyes, skin, and respiratory system. Furthermore, ethidium bromide which we use to detect DNA band is carcinogen. We also use mammalian cells in assay. These cells can be infected by viruses which can also infect us. We also use harmful reagent, such as membrane binding solution.</p>
-					<p>b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):</p>
-					<p>As maintained above, our lab has harmful organisms and substances. If they diffused outside, there might be health hazard.</p>
-					<p>c) Risks to the environment (from waste disposal, or from materials escaping from your lab)</p>
-					<p>We might dispose chips or tubes which contain a bit amount of genetically modified organisms without sterilizing. It offenses the law of our country; they may effect on biodiversity in our country. The bacteria we used also have drug resistance against ampicillin or CP, and if escaped, they could not be killed by those drugs. Mammalian cells are so weak that they can hardly live in the natural world.</p>
-					<p>d) Risks to security through malicious mis-use by individuals, groups, or countries</p>
-					<p>There seems to be no risks with this subject. In today's cognition, parts that we use do not seem to lead the expression of harmful materials.</p>
-					<p>e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</p>
-					<p>In order to avoid the risks about bacterial cells shown above, we autoclave all wastes, sterilize all equipments  that contain the organisms with detergent or hypochlorous acid. When bacteria exposed outside the tubes or examiners, we immediately seterilize them with ehtanol. Furthermore, in order to keep ethidium bromide away from our skin, we use kimwipes to wrap the chips used to taking it up before throwing away, and we take care that our bare hands do not touch the gel polluted by the substance.</p>
-					<h3>Risks of Your Project in the Future</h3>
-					<p>What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.</p>
-					<p>a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</p>
-					<p>In sigma-Recounter project, the circuit we constructed has potential for the application for defeating pests or bacteria by means of the expression of toxic proteins. In this case, there is a risk that you mistakenly output the toxin.<br />In CTCD project, the role of the circuits is to detect CTCs by GFP, thus it is thought to be difficult to have a risk of doing humans or environment harm.</p>
-					<p>b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.</p>
-					<p>In the future study of sigma-Recounter project, in addition to the reset system, we intend to increase the number of nodes and to enable one state to move to any other states. Therefore, if our project is used to express a toxin to defeat pest or bacteria, you can prepare an anti-toxin node or a reset system for mistakenly expressing the toxin.</p>
-				</div>
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 			<!-- Attribution -->
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-				<div id = "Attribution-1">
-					<img src="https://static.igem.org/mediawiki/2014/4/44/Sub_attribution.png" class = "contTitle" />
-					<p>Our activities involved in iGEM were all conducted by undergaduates alone!!<br>Based on fund-raisings and public relations, all team members had a lot of brainstoming, investigations and discussions in order to select project carefully. And we conducted experiments and FINALLY saw results.<br>We also designed and composed all publish tools, and of course, polished all scripts and presentation by ourselves.</p>
-					<h3>Project</h3>
-					<p>All we had a lot of brainstorming, investigations and discussion when we decide our projects.<br>σ-ReCounter:<br><b>Shunsuke Sumi</b>(idea)<br><b>So Nakashima</b>(idea and comformation)<br><b>Takefumi Yoshikawa</b>(comformation)<br><br>CTCD:<br><b>Masayuki Osawa</b>(conformation)<br><b>Shigetaka Kobari</b>(investigation and conformation)<br><b>Shunsuke Sumi</b>(conformation)<br><b>Senkei Hyo</b>(investigation)<br><b>Yoshihiko Tomofuji</b>(conformation)<br><b>Yshiki Okesaku</b>(investigation)</p>
-					<h3>Experiment</h3>
-					<p>Lab. Leader:<br><b>Takefumi Yoshikawa</b>(construction, assay;σ-ReCounter)<br><br>Lab. Members:<br><b>Atsuki Ito</b>(construction)<br><b>Hajime Takemura</b>(construction)<br><b>Keisuke Tsukada</b>(construction)<br><b>Kentaro Tara</b>(construction)<br><b>Kento Nakamura</b>(construction, assay;σ-ReCounter)<br><b>Naruki Yoshikawa</b>(construction)<br><b>Nobuhiro Hiura</b>(construction)<br><b>Shigetaka Kobari</b>(assay;CTCD)<br><b>So Nakashima</b>(construction, Assay;σ-ReCounter)<br><b>Yoshihiko Tomofuji</b>(assay;CTCD)<br><b>Yuto Yamanaka</b>(construction)</p>
-					<h3>Modeling</h3>
-					<p><b>Keisuke Tsukada, Kentaro Tara, Manabu Nishiura, Masaki Ono</b></p>
-					<h3>Web</h3>
-					<p>Almost all members wrote drafts of our team wiki.<br><b>Cristian David</b>(check our English)<br><b>Hiroki Tsuboi</b>(team website, implementation of our team wiki)</p>
-					<h3>App</h3>
-					<p><b>Naruki Yoshikawa</b></p>
-					<h3>Design</h3>
-					<p><b>Cristian David</b>(parker design)<br><b>Yoshiki Okesaku</b>(all design, all figure)</p>
-					<h3>Presentation</h3>
-					<p><b>Kento Nakamura, Manabu Nishiura, Masato Ishikawa, Yumeno Koga, Yuto Yamanaka</b></p>
-					<h3>Poster</h3>
-					<br>
-					<h3>Public Relation</h3>
-					<p><b>Ding Yuewen, Keisuke Tsukada</b></p>
-					<h3>Adviser</h3>
-					<p><b>Kota Tosimitsu</b></p>
-				</div>
-				<div id = "Attribution-2">
-					<img src="https://static.igem.org/mediawiki/2014/4/44/Sub_Ackno.png" class="contTitle" />
-				</div>
-				<div id = "Attribution-3">
-					<img src="https://static.igem.org/mediawiki/2014/3/38/Sub_sponsors.png" class="contTitle" />
-					<img src="https://static.igem.org/mediawiki/2014/7/72/Promega.png" class="sponsor">
-					<p><b>Promega KK.</b>for chamical reagents</p>
-					<p><b>Teiyukai, Faculty of Engineering, The University of Tokyo</b>for fund</p>
-					<p><b>Integrated DNA Technologies MBL</b></p>
-					<img src="https://static.igem.org/mediawiki/2014/3/30/Cosmobio.png" class="sponsor">
-					<p><b>COSMO BIO Co., Ltd.</b>for fund<br></p>
-					<img src="https://static.igem.org/mediawiki/2014/1/17/Liveanest.png" class="sponsor">
-					<p><b>Leave a Nest Co., Ltd.(Hiroyuki Takahashi)</b>for advice for public relations & introduction of Promega KK.</p>
-				</div>
 			</div>
 			<!-- Human Practice -->
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-				<div id = "Humanpractice-1">
-					<img src = "https://static.igem.org/mediawiki/2014/1/1c/Sub_introduction.png" class = "contTitle"/>
-					<p>This year, we set 2 goals in human practice and have made efforts to realize following goals.</p>
-					<p><b>1.Spreading iGEM in general public.</b></p>
-					<p><b>2.Activating iGEM in Japan.</b></p>
-					<p>We set these 2 goals because we want to remove prejudice against gene recombination and synthetic biology, and familiarize people with synthetic biology. By doing these, we think that people can understand synthetic biology better.</p>
-					<p>We also expect undergraduate students, who will lead next generation of biology, to improve their skills by activating iGEM in Japan.</p>				</div>
-				<div id = "Humanpractice-2">
-					<img src="https://static.igem.org/mediawiki/2014/4/4b/Sub_to_spread_igem_in_general_public.png" class = "contTitle" />
-					<h3>"EcoLightsOut"</h3>
-					<p>We have developed an Android app. to introduce our project. This is a puzzle game which uses the counting system we created. In the project, the state of E. coli is changed by stimulus of signal molecules. In the game, players can stimulate the E. coli by touching it. E. coli are displayed in 3x3 or 5x5 grid shape. When you touch an E. coli in the game, the colors of the E. coli and four adjacent ones are changed. This behavior is analogy of diffusion of signal molecules. The goal is to turn all E. coli green.</p>
-					<p>Synthetic biology is not well-known and thought to be unfamiliar to ordinary people. To introduce synthetic biology into public, an easy entrance such as playing game is effective. We hope that players will get interested in synthetic biology by enjoying this game.</p>
-					<p>You can download this game from Google Play.</p>
-					<h3>Lectures to general public</h3>
-					<h4>School festivals</h4>
-					<p>The university of Tokyo has two school festivals per year. The May festival is held in May and the Komaba festival was held in November.We explained iGEM and synthetic biology briefly and introduce our project to audience. We　invited other iGEM teams in Japan to May festival.  We offered precious opportunities that Japanese iGEM teams meet through May festivals.</p>
-					<h4>Techno-Edge</h4>
-					<p>Techno-Edge is the event which the department of technology of university of Tokyo held. The purpose of this event was to appeal department of technology to junior high or high school students.Many laboratories and academic circles such as Robotech took part in this event. iGEM UT-Tokyo also participated in it to appeal synthetic biology.</p>
-					<p>Not only high school and junior high, but also primary school students came to our booth and were interested in our explanation.</p>
-					<h4>Presentation</h4>
-					<p>This year, iGEM Nagahama invited us to the genetics society of Japan, and we participated in it. We made an oral presentation workshop of synthetic biology and took part in poster session. There were many iGEM teams, and we could advertise iGEM in academic world. Moreover, specialists gave advice to us, and we were inspired by professors of synthetic biology.</p>
-					<p>We also joined in Japanese Society for Cell Synthesis Research.</p>
-					<h4>A cram school</h4>
-					<p>We held a seminar in which we explained synthetic biology and iGEM for high school students at a cram school in Komaba.</p>
-				</div>
-				<div id = "Humanpractice-3">
-					<img src="https://static.igem.org/mediawiki/2014/3/34/Sub_to_activate_igem.png" class = "contTitle" />
-					<h3>Collaborations</h3>
-					<p>We collaborated on modeling with Nagahama.</p>
-					<p>Their project aimed cadmium collection using <i>Escherichia coli</i> which has positive chemotaxis for aspartic acid, then we constructed simplified model of chemotaxis
-					and simulated behavior of <i>E. coli</i> by using probability and random function.</p>
-					<p>Their wiki explained the result of modeling.(→link) We constructed all equations in their simulation including the equation that determines <i>E. coli</i> to choose going straight or turning in probability, and explained equations to them.The figure describing the result of simulation is also made by UT-Tokyo. All code for modeling is here.(→link)</p>
-					<h3>Ethics and regulation</h3>
-					<p>We thought to confirm whether our project meets ethics, but ethics is very vague, so we decided to research about ethics.</p>
-					<h3>iGEM Japan</h3>
-					<p>iGEM Japan is an organization which was founded last year for iGEM teams in Japan to cooperate each other.</p>
-					<p>In March, iGEM Kyoto held iGEM-Japan West meeting. In this meeting, we shared each team's project and advised each other. This meeting was very useful because we could find our idea's weak point.</p>
-					<p>In August, iGEM TMU-Tokyo held iGEM-Japan East meeting, and we made presentations about our projects and criticized each other. Thanks to these meetings, we developed quality of our projects.</p>
-				</div>
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 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YOICHI IRIE</p>
 							<img src = "https://static.igem.org/mediawiki/2014/6/6a/Irie_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yoichi Irie</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Arts and Sciences</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Team leader</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">My dream is to make me robuster against stress caused by iGEM in synthetic biology!</p>
 						</div>
 					</div>
@@ Line 297: / Line 127: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name" style="font-size:35px;">CRISTIAN DAVID PENA MARINEZ</p>
 							<img src = "https://static.igem.org/mediawiki/2014/f/f1/Chris_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Cristian David Pena Martinez</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Design</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">"The only concrete proof of the human existence is: poetry" - Luis Cardoza y Aragon</p>
 						</div>
 					</div>
@@ Line 327: / Line 157: @@
 								<dd>Masaki Ono</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Arts and Sciences, Sophmore</dd>
-								<dt>Job</dt>
-								<dd>Modeling</dd>
-							</dl>
-							<p class ="comment">I want to be a doctor.</p>
-						</div>
-					</div>
-				</div>
-				<div class = "element-item">
-					<div class = "thumbnail">
-						<img src = "https://static.igem.org/mediawiki/2014/a/a1/Ishikawa.png" class ="member-thumbnail" />
-					</div>
-					<div class = "detail">
-						<div class = "meta">
-							<p>iGEM UT-Tokyo</p>
-							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
-						</div>
-						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
-							<img src = "https://static.igem.org/mediawiki/2014/7/78/Cocoa_comb.png" class ="member-detail" />
-							<dl class = "profile">
-								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
-								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
 								<dt>Job</dt>
 								<dd>Modeling</dd>
@@ Line 369: / Line 175: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YUMENO KOGA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/6/63/Koga_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yumeno Koga</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Lifescience & Medical Bioscience, School of Advanced Science and Engineering, Waseda University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Presenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I'm a  "wasejo".</p>
 						</div>
 					</div>
@@ Line 393: / Line 199: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KENTO NAKAMURA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/8/87/Nakamura_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Kento Nakamura</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experimenter, Presenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">"I think, therefore I am" by R. Descartes<br />"I multiply, therefore I am" by E.coli <br />"I hope so..." by Experimenter</p>
 						</div>
 					</div>
@@ Line 417: / Line 223: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">SOH NAKASHIMA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/7/74/Dolicas_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Soh Nakashima</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experimenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">follow me on Twitter(<a href="https://twitter.com/dolicas_" target="_blank" >@dolicas_</a>)</p>
 						</div>
 					</div>
@@ Line 441: / Line 247: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">MANABU NISHIURA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/2/22/Nishiura_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Manabu Nishiura</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Arts and Sciences</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Modeling,Presenter</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment"> I am a Feynman Diagram.</p>
 						</div>
 					</div>
@@ Line 465: / Line 271: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YOSHIKI OKESAKU</p>
 							<img src = "https://static.igem.org/mediawiki/2014/d/d0/Oke_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yoshiki Okesaku</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd> Department of Physics, Graduate School of Science, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Design(DOKATA)</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I am majoring in the MDS theory. The MDS theory can describe how our universe had begun and will end.</p>
 						</div>
 					</div>
@@ Line 489: / Line 295: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">HAJIME TAKEMURA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/3/35/Takemura_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Hajime Takemura</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The University of Tokyo, Junior,Faculty of Pharmaceutical  Science</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experiment</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I want to make a new medicine.</p>
 						</div>
 					</div>
@@ Line 513: / Line 319: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KENTARO TARA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/e/ef/Tara_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Kentaro Tara</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Arts and Sciences, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experiment, Modeling</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">Maps relax me.</p>
 						</div>
 					</div>
@@ Line 537: / Line 343: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YUEWEN DING</p>
 							<img src = "https://static.igem.org/mediawiki/2014/0/0e/Tei_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yuewen Ding</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Faculty of Pharmaceutical  Science, The university of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Public relation</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">Eating is sweeter than Life.</p>
 						</div>
 					</div>
@@ Line 561: / Line 367: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YOSHIHIKO TOMOFUJI</p>
 							<img src = "https://static.igem.org/mediawiki/2014/2/21/Tomohuji_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yoshihiko Tomofuji</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>The university of Tokyo, faculty of medicine</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>E.coli</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I'm a E.coli.</p>
 						</div>
 					</div>
@@ Line 585: / Line 391: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KOHTA TOSHIMITSU</p>
 							<img src = "https://static.igem.org/mediawiki/2014/7/7c/Toshimitsu_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Kohta Toshimitsu</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Faculty of Pharmacy,　Keio University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Adviser</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">I'm so sick of pipetting.</p>
 						</div>
 					</div>
@@ Line 609: / Line 415: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">KEISUKE TSUKADA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/0/0b/Tsukada_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Keisuke Tsukada</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Biophysics and Biochemistry , Faculty of Science, The University of Tokyo</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experiment, Modeling</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
+							<p class ="comment">itininnmaenokenkyuusyaninaretaraiina</p>
 						</div>
 					</div>
@@ Line 633: / Line 439: @@
 						</div>
 						<div class = "member">
-							<p class = "name">MASAKI ONO</p>
+							<p class = "name">YUTO YAMANAKA</p>
 							<img src = "https://static.igem.org/mediawiki/2014/2/26/Yamanaka_comb.png" class ="member-detail" />
 							<dl class = "profile">
 								<dt>Name</dt>
-								<dd>Masaki Ono</dd>
+								<dd>Yuto Yamanaka</dd>
 								<dt>Belong to</dt>
-								<dd>The University of Tokyo, Literature, Sophmore</dd>
+								<dd>Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University</dd>
 								<dt>Job</dt>
-								<dd>Modeling</dd>
+								<dd>Experimenter, Presenter</dd>
+							</dl>
+							<p class ="comment">I like Falcon tubes.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/3/3e/Sumi.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">SHUNSUKE SUMI</p>
+							<img src = "https://static.igem.org/mediawiki/2014/e/e8/Sumi_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Shunsuke Sumi</dd>
+								<dt>Belong to</dt>
+								<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>
+								<dt>Job</dt>
+								<dd>tree</dd>
+							</dl>
+							<p class ="comment">a tree has no emotion.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/3/34/Yoshikawa%281st%29.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">NARUKI YOSHIKAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/d/de/Yoshikawa%281st%29_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Naruki Yoshikawa</dd>
+								<dt>Belong to</dt>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Experimenter</dd>
+							</dl>
+							<p class ="comment">Three billion devices run Java, but my devices don't.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/9/95/Hiura.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">NOBUHIRO HIURA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/e/ea/Hiura_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Nobuhiro Hiura</dd>
+								<dt>Belong to</dt>
+								<dd>Department of Regulatory Biology, Faculty of Science, Saitama University</dd>
+								<dt>Job</dt>
+								<dd>Experimenter</dd>
+							</dl>
+							<p class ="comment">Let's go to Saitama, Japan!</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/8/8c/Tsuboi.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">HIROKI TSUBOI</p>
+							<img src = "https://static.igem.org/mediawiki/2014/b/b6/Tsuboi_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Hiroki Tsuboi</dd>
+								<dt>Belong to</dt>
+								<dd>Waseda University, School of Fundamental Science and Engineering, Computer Science and Engineering</dd>
+								<dt>Job</dt>
+								<dd>Web</dd>
+							</dl>
+							<p class ="comment">After wiki freeze, I will make other Web Site</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/2/2b/Kobari.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">SHIGETAKA KOBARI</p>
+							<img src = "https://static.igem.org/mediawiki/2014/f/fa/Kobari_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Shigetaka Kobari</dd>
+								<dt>Belong to</dt>
+								<dd>The university of Tokyo, faculty of medicine</dd>
+								<dt>Job</dt>
+								<dd>tree</dd>
+							</dl>
+							<p class ="comment">a tree has no emotion.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/a/a1/Ishikawa.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">MASATO ISHIKAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/e/e9/Ishikawa_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Masato Ishikawa</dd>
+								<dt>Belong to</dt>
+								<dd>College of Arts and Sciences, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Presenter</dd>
+							</dl>
+							<p class ="comment">I want to study pharmacy.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/c/c6/Osawa.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">MASAYUKI OSAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/1/12/Osawa_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Masayuki Osawa</dd>
+								<dt>Belong to</dt>
+								<dd>Department of medicine, Faculty of medicine, The Jikei University School of Medicine</dd>
+								<dt>Job</dt>
+								<dd>Woodkeeper</dd>
+							</dl>
+							<p class ="comment">I take care of crazy tree.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/5/53/Tube.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">TAKEHUMI YOSHIKAWA</p>
+							<img src = "https://static.igem.org/mediawiki/2014/b/b1/Tube_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Takehumi Yoshikawa</dd>
+								<dt>Belong to</dt>
+								<dd>Department of Bioinformatics and Systems Biology, Faculty of Science, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Experiment Leader</dd>
+							</dl>
+							<p class ="comment">I want to be Spe1.</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/d/d4/Ito.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">ATSUKI ITO</p>
+							<img src = "https://static.igem.org/mediawiki/2014/1/1d/Ito_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Atsuki Ito</dd>
+								<dt>Belong to</dt>
+								<dd>Department of Bioscience, Faculty of Engineering,Tokyo University of Science</dd>
+								<dt>Job</dt>
+								<dd>Experimenter</dd>
+							</dl>
+							<p class ="comment">kimuwaipu,azinai...</p>
+						</div>
+					</div>
+				</div>
+				<div class = "element-item">
+					<div class = "thumbnail">
+						<img src = "https://static.igem.org/mediawiki/2014/3/3e/Prof_wakamoto.png" class ="member-thumbnail" />
+					</div>
+					<div class = "detail">
+						<div class = "meta">
+							<p>iGEM UT-Tokyo</p>
+							<img src = "https://static.igem.org/mediawiki/2014/9/98/Ui_close_40.png" class = "closebutton" />
+						</div>
+						<div class = "member">
+							<p class = "name">PROF.WAKAMOTO</p>
+							<img src = "https://static.igem.org/mediawiki/2014/f/fb/Prof_wakamoto_comb.png" class ="member-detail" />
+							<dl class = "profile">
+								<dt>Name</dt>
+								<dd>Yuichi Wakamoto</dd>
+								<dt>Belong to</dt>
+								<dd>Center for Complex Systems Biology The University of Tokyo, Dapartment of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo</dd>
+								<dt>Job</dt>
+								<dd>Instructor</dd>
 							</dl>
-							<p class ="comment">I want to be a doctor.</p>
 						</div>
 					</div>
@@ Line 649: / Line 694: @@
 			</div>
 			</div>
-			<!-- Teamのトップここまで -->
 			<!-- 上へ戻るボタン -->
 			<div id="bttop" style="display:none;">
 				<a href="Javascript:bttop()"><img src="https://static.igem.org/mediawiki/2014/0/06/Topbutton.png" /></a>
 			</div>
-			<!-- 上へ戻るボタン -->
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Team:UT-Tokyo/CTCD/Content

From 2014.igem.org

Latest revision as of 03:02, 18 October 2014