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Team:NCTU Formosa/Notebook/test6
From 2014.igem.org
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<div class="calendar"> | <div class="calendar"> | ||
<ul class="calendar-week"> | <ul class="calendar-week"> | ||
| - | <li> | + | <li>Sun.</li> |
| - | <li> | + | <li>Mon.</li> |
| - | <li> | + | <li>Tue.</li> |
| - | <li> | + | <li>Wed.</li> |
| - | <li> | + | <li>Thu.</li> |
| - | <li> | + | <li>Fri.</li> |
| - | <li> | + | <li>Sat.</li> |
</ul> | </ul> | ||
<ul class="calendar-day"> | <ul class="calendar-day"> | ||
| Line 69: | Line 69: | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 9/4</p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/4</span></font><br></p> |
<p> | <p> | ||
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p> |
<p> | <p> | ||
| - | + | P<sub>cons</sub>+RBS+AseI PCR product-1,2,3,4,7,8 Ar</p> | |
<p> | <p> | ||
XhoI+PSB1K3 PCR product-1,2 Kr</p> | XhoI+PSB1K3 PCR product-1,2 Kr</p> | ||
| Line 79: | Line 79: | ||
PSB1A3 Ar</p> | PSB1A3 Ar</p> | ||
<p> | <p> | ||
| - | Cultivation plate test | + | <font color="yellow">Cultivation plate test</font></p> |
<p> | <p> | ||
Ar plate and Kr plate</p> | Ar plate and Kr plate</p> | ||
<p> | <p> | ||
| - | Digestion of | + | <font color="yellow">Digestion of</font></p> |
<p> | <p> | ||
| - | [PBAN-HindIII] Kr*2 (EP) | + | [PBAN-HindIII] Kr*2 (EP)</p><p> |
| + | [PBAN-NheI]Kr*2 (EP)</p><p> | ||
| + | [PBAN-KpnI-HF]Kr*2 (EP)</p> | ||
<p> | <p> | ||
| - | [23101+B0034+PBAN (HindIII)]Ar(ES) | + | [23101+B0034+PBAN (HindIII)]Ar(ES)</p><p> |
| + | [23101+B0034+PBAN (NheI)]Ar(ES)</p> | ||
<p> | <p> | ||
| - | PCR of | + | <font color="yellow">PCR of</font></p> |
<p> | <p> | ||
[J23101+B0034+KpnI-HF] Ar*3</p> | [J23101+B0034+KpnI-HF] Ar*3</p> | ||
<p> | <p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of</font></p> |
<p> | <p> | ||
[J23101+B0034+KpnI-HF+PSB1C3]</p> | [J23101+B0034+KpnI-HF+PSB1C3]</p> | ||
<p> | <p> | ||
| - | Electrophoresis of | + | <font color="yellow">Electrophoresis of</font></p> |
<p> | <p> | ||
[PBAN-HindIII] Kr*2 (EP)</p><p> | [PBAN-HindIII] Kr*2 (EP)</p><p> | ||
| Line 109: | Line 112: | ||
[23101+B0034+PBAN (KpnI-HF)]Ar PCR*3</p> | [23101+B0034+PBAN (KpnI-HF)]Ar PCR*3</p> | ||
<p> | <p> | ||
| - | 9/5</p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/5</span></font><br></p> |
<p> | <p> | ||
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | Well 1 | + | Well 1 P<sub>cons</sub>+RBS+AseI--1 (EP) Ar</p><p> |
| - | Well 2 | + | Well 2 P<sub>cons</sub>+RBS+AseI--2 (EP) Ar</p><p> |
| - | Well 3 | + | Well 3 P<sub>cons</sub>+RBS+AseI--3 (EP) Ar</p><p> |
| - | Well 4 | + | Well 4 P<sub>cons</sub>+RBS+AseI--4 (EP) Ar</p><p> |
| - | Well 5 | + | Well 5 P<sub>cons</sub>+RBS+AseI--7 (EP) Ar （DNA sequencing）</p><p> |
| - | Well 6 | + | Well 6 P<sub>cons</sub>+RBS+AseI--8 (EP) Ar</p><p> |
| - | Well 7 | + | Well 7 P<sub>cons</sub>+RBS+NcoI--2 (EP) Cr （DNA sequencing）</p><p> |
| - | Well 8 | + | Well 8 P<sub>cons</sub>+RBS+Ndel--2 (EP) Cr （DNA sequencing）</p><p> |
Well 9 1 Kb marker</p><p> | Well 9 1 Kb marker</p><p> | ||
| - | Well 10 | + | Well 10 P<sub>cons</sub>+RBS+Ndel--13 (EP) Cr</p><p> |
Well 11 SacI--3 (XP) Kr</p><p> | Well 11 SacI--3 (XP) Kr</p><p> | ||
Well 12 SacI--4.1 (XP) Kr</p><p> | Well 12 SacI--4.1 (XP) Kr</p><p> | ||
| Line 128: | Line 131: | ||
Well 15 XhoI--8 (EP) Kr（DNA sequencing）</p><p> | Well 15 XhoI--8 (EP) Kr（DNA sequencing）</p><p> | ||
Well 16 SalI--3 (XP) Kr</p><p> | Well 16 SalI--3 (XP) Kr</p><p> | ||
| - | Mini prep of | + | <font color="yellow">Mini-prep of</font> </p><p> |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF-7</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF-8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF-9</p><p> | |
| - | Digestion of | + | <font color="yellow">Digestion of</font> </p><p> |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF-7 (EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF-8 (EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF-9 (EP)</p><p> | |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font> </p><p> |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF Ar PCR product-7,8,9 Ar</p><p> | |
| - | Ligation of | + | <font color="yellow">Ligation of</font> </p><p> |
[J23101+B0034+HindIII+B0034+PSB1K3] Kr</p><p> | [J23101+B0034+HindIII+B0034+PSB1K3] Kr</p><p> | ||
[J23101+B0034+HindIII+J61048+PSB1K3] Kr</p><p> | [J23101+B0034+HindIII+J61048+PSB1K3] Kr</p><p> | ||
[J23101+B0034+NheI+B0034+PSB1K3] Kr</p><p> | [J23101+B0034+NheI+B0034+PSB1K3] Kr</p><p> | ||
[J23101+B0034+NheI+J61048+PSB1K3] Kr</p><p> | [J23101+B0034+NheI+J61048+PSB1K3] Kr</p><p> | ||
| - | Electrophoresis of digested product | + | <font color="yellow">Electrophoresis of</font> digested product of</p><p> |
| - | [ | + | [P<sub>cons</sub>+RBS+KpnI-HF]Ar (EP)*3 </p><p> |
</p> | </p> | ||
| Line 150: | Line 153: | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 9/9</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/9</span></font><br></p><p> |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+SacI PCR product-16 Ar</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SaII PCR product-17 Ar</p><p> | |
RBS+BFP+Ter PCR product-16,17,29,30 Ar</p><p> | RBS+BFP+Ter PCR product-16,17,29,30 Ar</p><p> | ||
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
XhoI+PSB1K3 (EP)</p><p> | XhoI+PSB1K3 (EP)</p><p> | ||
| - | 9/10</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/10</span></font><br></p><p> |
| - | Mini prep of | + | <font color="yellow">Mini-prep of</font></p><p> |
RBS+BFP+Ter-16 Ar</p><p> | RBS+BFP+Ter-16 Ar</p><p> | ||
RBS+BFP+Ter-17 Ar</p><p> | RBS+BFP+Ter-17 Ar</p><p> | ||
RBS+BFP+Ter-29 Ar</p><p> | RBS+BFP+Ter-29 Ar</p><p> | ||
RBS+BFP+Ter-30 Ar</p><p> | RBS+BFP+Ter-30 Ar</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+Sac-16 Ar</p><p> | |
| - | + | P<sub>cons</sub>+RBS+Sal-17 Ar</p><p> | |
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
RBS+BFP+Ter-16 Ar(EP)</p><p> | RBS+BFP+Ter-16 Ar(EP)</p><p> | ||
RBS+BFP+Ter-17 Ar(EP)</p><p> | RBS+BFP+Ter-17 Ar(EP)</p><p> | ||
RBS+BFP+Ter-29 Ar(EP)</p><p> | RBS+BFP+Ter-29 Ar(EP)</p><p> | ||
RBS+BFP+Ter-30 Ar(EP)</p><p> | RBS+BFP+Ter-30 Ar(EP)</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI-16 Ar(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI-17 Ar(EP)</p><p> | |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+SacI-16 dig(EP)</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+SalI-17 dig(EP)</p><p> | |
| - | + | Well 4 RBS+BFP+Ter-16 dig(EP)</p><p> | |
| - | + | Well 5 RBS+BFP+Ter-17 dig(EP)</p><p> | |
| - | + | Well 6 RBS+BFP+Ter-29 dig(EP)</p><p> | |
| - | + | Well 7 RBS+BFP+Ter-30 dig(EP)</p><p> | |
| - | + | Well 8 XhoI-7 dig(XP) Kr</p><p> | |
| - | 9/11</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/11</span></font><br></p><p> |
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+PBAN(ES)(8 kind except of XhoI)</p><p> | |
RBS+BFP+Ter(XP)</p><p> | RBS+BFP+Ter(XP)</p><p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+XhoI-7 Ar</p><p> | |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+AseI-7 Ar</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-16 Ar</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI-17 Ar</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI-2 Ar</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI-2 Ar</p><p> | |
RBS+BFP+Ter-2 Ar</p><p> | RBS+BFP+Ter-2 Ar</p><p> | ||
RBS+BFP+Ter-2 Ar</p><p> | RBS+BFP+Ter-2 Ar</p><p> | ||
SalI-3 Kr</p><p> | SalI-3 Kr</p><p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+XhoI-7 Ar</p><p> | |
| - | DNA sequencing of | + | <font color="yellow">DNA sequencing of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+SaeI Ar 8ul VF2</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI Ar 8ul VF2</p><p> | |
RBS+BFP+Ter-29 Ar 8ul VF2 + 8ul VR</p><p> | RBS+BFP+Ter-29 Ar 8ul VF2 + 8ul VR</p><p> | ||
RBS+BFP+Ter-30 Ar 5ul VF2 + 5ul VR</p><p> | RBS+BFP+Ter-30 Ar 5ul VF2 + 5ul VR</p><p> | ||
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+HindIII dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI dig(EP)-->9/11 mini</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI dig(EP)</p><p> | |
| - | Ligation of | + | <font color="yellow">Ligation of</font> </p><p> |
| - | + | P<sub>cons</sub>+RBS+BFP+Ter-30 Kr</p><p> | |
| - | 9/12</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/12</span></font><br></p><p> |
| - | Transformation of | + | <font color="yellow">Transformation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+BFP+Ter Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI Ar</p><p> | |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+NdeI dig(EP) Cr</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+NcoI dig(EP) Cr</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+SacI dig(EP) Ar</p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+SalI dig(EP) Ar</p><p> | |
| - | + | Well 6 P<sub>cons</sub>+RBS+AseI dig(EP) Ar</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+HindIII dig(EP) Ar</p><p> | |
| - | + | Well 8 P<sub>cons</sub>+RBS+NheI dig(EP) Ar</p><p> | |
| - | + | Well 9 P<sub>cons</sub>+RBS+KpnI dig(EP) Ar</p> | |
<p> | <p> | ||
| - | + | <font color="#FF0000"><span style="font-size:1cm;">9/13</span></font><br></p><p> | |
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+AseI Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI Kr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI Kr</p><p> | |
| - | PCR of | + | <font color="yellow">PCR of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-HF+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+Xhol Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter Kr x16</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter Kr x16</p><p> | |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
Gel 1</p><p> | Gel 1</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter Kr x16</p><p> | |
Gel 2</p><p> | Gel 2</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter Kr x16</p><p> | |
Gel 3</p><p> | Gel 3</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Kr x8</p><p> | |
Gel 4</p><p> | Gel 4</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Kr x8</p><p> | |
Gel 5</p><p> | Gel 5</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+KpnI-HF+RBS+BFP+Ter Kr x8</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Kr x8</p><p> | |
Gel 6</p><p> | Gel 6</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+Xhol Kr x8</p><p> | |
psb1K3 dig(EP)</p> | psb1K3 dig(EP)</p> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| - | <p> | + | <p><font color="#FF0000"><span style="font-size:1cm;">9/16</span></font><br></p><p> |
| - | + | <font color="yellow">Transformation of</font></p><p> | |
| - | Transformation of</p><p> | + | |
psb3K3 *2</p><p> | psb3K3 *2</p><p> | ||
psb3C3</p><p> | psb3C3</p><p> | ||
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
psb3K3 dig(EP)</p><p> | psb3K3 dig(EP)</p><p> | ||
psb1C3 dig(EP)</p><p> | psb1C3 dig(EP)</p><p> | ||
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter x8</p> | |
<p> | <p> | ||
| - | 9/17</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/17</span></font><br></p><p> |
| - | Mini prep of | + | <font color="yellow">Mini-prep of</font></p><p> |
PBAN：XhoI</p><p> | PBAN：XhoI</p><p> | ||
SalI</p><p> | SalI</p><p> | ||
| Line 296: | Line 298: | ||
NheI</p><p> | NheI</p><p> | ||
HindIII (fail)</p><p> | HindIII (fail)</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter：AseI-4</p><p> | |
Ndel-8</p><p> | Ndel-8</p><p> | ||
HindIII-4 (fail)</p><p> | HindIII-4 (fail)</p><p> | ||
| Line 303: | Line 305: | ||
NcoI-2</p><p> | NcoI-2</p><p> | ||
NheI-1</p><p> | NheI-1</p><p> | ||
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
PBAN：XhoI</p><p> | PBAN：XhoI</p><p> | ||
SalI</p><p> | SalI</p><p> | ||
| Line 312: | Line 314: | ||
NcoI</p><p> | NcoI</p><p> | ||
AseI</p><p> | AseI</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter：AseI-4</p><p> | |
Ndel-8</p><p> | Ndel-8</p><p> | ||
KpnI-1</p><p> | KpnI-1</p><p> | ||
NcoI-2</p><p> | NcoI-2</p><p> | ||
NheI-1</p><p> | NheI-1</p><p> | ||
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (Ndel)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (NcoI)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (SacI)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (HindIII)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (SalI)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (NheI)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (KpnI)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (AseI)</p><p> | |
PBAN AseI</p><p> | PBAN AseI</p><p> | ||
PBAN Ndel</p><p> | PBAN Ndel</p><p> | ||
| Line 332: | Line 334: | ||
PBAN SalI</p><p> | PBAN SalI</p><p> | ||
PBAN XhoI</p><p> | PBAN XhoI</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+XhoI-1 dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+Xhol-1 dig(ES)</p><p> | |
psb1C3 dig(ES)</p><p> | psb1C3 dig(ES)</p><p> | ||
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (SacI)-4.12</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (HindIII)-6</p><p> | |
| - | + | P<sub>cons</sub>+RBS+PBAN+RBS+BFP+Ter (SalI)-9</p><p> | |
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
B0034 Kr dig(ES)</p><p> | B0034 Kr dig(ES)</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)</p><p> | |
NcoI Kr dig(EP)</p><p> | NcoI Kr dig(EP)</p><p> | ||
Ndel Kr dig(EP)</p><p> | Ndel Kr dig(EP)</p><p> | ||
SalI Kr dig(EP)</p><p> | SalI Kr dig(EP)</p><p> | ||
| - | 9/18</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/18</span></font><br></p><p> |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | Well | + | Well 1 Marker</p><p> |
| - | + | Well 2 B0034 Kr dig(ES)</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 5 NcoI Kr dig(EP)</p><p> | |
| - | + | Well 6 Ndel Kr dig(EP)</p><p> | |
| - | + | Well 7 SalI Kr dig(EP)</p><p> | |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 B0034 Kr dig(ES)</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 5 NcoI Kr dig(EP)</p><p> | |
| - | + | Well 6 Ndel Kr dig(EP)</p><p> | |
| - | + | Well 7 SalI Kr dig(EP)</p><p> | |
| - | Mini prep | + | <font color="yellow">Mini-prep of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter-6</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter-9</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter-4</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter-11</p><p> | |
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Cr</p><p> | |
NheI Cr</p><p> | NheI Cr</p><p> | ||
NcoI Cr</p> | NcoI Cr</p> | ||
<p> | <p> | ||
| - | 9/20</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/20</span></font><br></p><p> |
| - | Transformation of | + | <font color="yellow">Transformation of</font></p><p> |
AseI mini Cr</p><p> | AseI mini Cr</p><p> | ||
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 HindIII-2 Kr dig(EP)</p><p> | |
| - | + | Well 3 SacI Kr dig(EP)</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+HindIII-6+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+SacI-11+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 6 P<sub>cons</sub>+RBS+SacI-9+RBS+BFP+Ter Kr dig(EP)</p><p> | |
| - | + | Well 7 RBS+BFP+Ter Ar dig(XP)</p><p> | |
| - | + | Well 8 P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Kr dig(EP)</p> | |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 9/21</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/21</span></font><br></p><p> |
| - | Electrophoresis of PCR products | + | <font color="yellow">Electrophoresis of</font> PCR products of</p><p> |
Gel 1</p><p> | Gel 1</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 3H1</p><p> | |
| - | + | Well 3 3H2</p><p> | |
| - | + | Well 4 3H3</p><p> | |
| - | + | Well 5 3H4</p><p> | |
| - | + | Well 6 3H5</p><p> | |
| - | + | Well 7 3H6</p><p> | |
| - | + | Well 8 Marker</p><p> | |
| - | + | Well 9 6K1</p><p> | |
| - | + | Well 10 6K2</p><p> | |
| - | + | Well 11 6K3</p><p> | |
| - | + | Well 12 6K4</p><p> | |
| - | + | Well 13 6K5</p><p> | |
| - | + | Well 14 6K6</p><p> | |
| - | + | Well 15 3Sl1</p><p> | |
| - | + | Well 16 3Sl2</p><p> | |
| - | + | Well 17 3Sl3</p><p> | |
Gel 2</p><p> | Gel 2</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 6Sl4</p><p> | |
| - | + | Well 3 6Sl5</p><p> | |
| - | + | Well 4 3Nh1</p><p> | |
| - | + | Well 5 3Nh2</p><p> | |
| - | + | Well 6 3Nh3</p><p> | |
| - | + | Well 7 3Nh4</p><p> | |
| - | + | Well 8 Marker</p><p> | |
| - | + | Well 9 3Nh5</p><p> | |
| - | + | Well 10 3Nh6</p><p> | |
| - | + | Well 11 6X1</p><p> | |
| - | + | Well 12 6X2</p><p> | |
| - | + | Well 13 6X3</p><p> | |
| - | + | Well 14 6X4</p><p> | |
| - | + | Well 15 6X5</p><p> | |
| - | + | Well 16 6X6</p><p> | |
| - | + | Well 17 1K1</p><p> | |
Gel 3</p><p> | Gel 3</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 1K2</p><p> | |
| - | + | Well 3 1K3</p><p> | |
| - | + | Well 4 1K4</p><p> | |
| - | + | Well 5 1K5</p><p> | |
| - | + | Well 6 1K6</p><p> | |
| - | + | Well 7 1X1</p><p> | |
| - | + | Well 8 Marker</p><p> | |
| - | + | Well 9 1X2</p><p> | |
| - | + | Well 10 1X3</p><p> | |
| - | + | Well 11 1X4</p><p> | |
| - | + | Well 12 1X5</p><p> | |
| - | + | Well 13 1X6</p><p> | |
| - | + | Well 14 3K1</p><p> | |
| - | + | Well 15 3K2</p><p> | |
| - | + | Well 16 3K3</p><p> | |
| - | + | Well 17 3K4</p><p> | |
Gel 4</p><p> | Gel 4</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 3K5</p><p> | |
| - | + | Well 3 3K6</p><p> | |
| - | + | Well 4 3Sc1</p><p> | |
| - | + | Well 5 3Sc2</p><p> | |
| - | + | Well 6 3Sc3</p><p> | |
| - | + | Well 7 3Sc4</p><p> | |
| - | + | Well 8 Marker</p><p> | |
| - | + | Well 9 3Sc5</p><p> | |
| - | + | Well 10 3Sc6</p><p> | |
| - | + | Well 11 1Sc1</p><p> | |
| - | + | Well 12 1Sc2</p><p> | |
| - | + | Well 13 1Sc3</p><p> | |
| - | + | Well 14 1Sc4</p><p> | |
| - | + | Well 15 1Sc5</p><p> | |
| - | + | Well 16 1Sc6</p><p> | |
| - | + | Well 17 1Nc1</p><p> | |
Gel 5</p><p> | Gel 5</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 1Nc2</p><p> | |
| - | + | Well 3 1Nc3</p><p> | |
| - | + | Well 4 1Nc4</p><p> | |
| - | + | Well 5 1Nc5</p><p> | |
| - | + | Well 6 1Nc6</p><p> | |
| - | + | Well 7 1A1</p><p> | |
| - | + | Well 8 Marker</p><p> | |
| - | + | Well 9 1A2</p><p> | |
| - | + | Well 10 1A3</p><p> | |
| - | + | Well 11 1A4</p><p> | |
| - | + | Well 12 1A5</p><p> | |
| - | + | Well 13 1A6</p><p> | |
| - | + | Well 14 1Nd1</p><p> | |
| - | + | Well 15 1Nd2</p><p> | |
| - | + | Well 16 1Nd3</p><p> | |
| - | + | Well 17 1Nd4</p><p> | |
Gel 6</p><p> | Gel 6</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 1Nd5</p><p> | |
| - | + | Well 3 1Nd6</p><p> | |
| - | + | Well 4 3X1</p><p> | |
| - | + | Well 5 3X2</p><p> | |
| - | + | Well 6 3X3</p><p> | |
| - | + | Well 7 3X4</p><p> | |
| - | + | Well 8 Marker</p><p> | |
| - | + | Well 9 3X5</p><p> | |
| - | + | Well 10 3X6</p><p> | |
| - | + | Well 11 3A1</p><p> | |
| - | + | Well 12 3A2</p><p> | |
| - | + | Well 13 3A3</p><p> | |
| - | + | Well 14 3A4</p><p> | |
| - | + | Well 15 3A5</p><p> | |
| - | + | Well 16 3A6</p><p> | |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
KpnI-1</p><p> | KpnI-1</p><p> | ||
NdeI-1</p><p> | NdeI-1</p><p> | ||
| Line 512: | Line 514: | ||
SacI-1</p><p> | SacI-1</p><p> | ||
AseI-1</p><p> | AseI-1</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+HindIII-1</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-4</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI-4</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI-5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI-5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI-1</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-1</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-1+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI-6+RBS+BFP+Ter</p><p> | |
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
SalI Kr dig(EP)</p><p> | SalI Kr dig(EP)</p><p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
NdeI dig(EP)+C backbone</p><p> | NdeI dig(EP)+C backbone</p><p> | ||
| - | 9/22</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/22</span></font><br></p><p> |
| - | Transformation of | + | <font color="yellow">Transformation of</font></p><p> |
NheI Cr</p><p> | NheI Cr</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+Ndel+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Cr</p><p> | |
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
HindIII(EP)</p><p> | HindIII(EP)</p><p> | ||
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
SacI-3 Kr</p><p> | SacI-3 Kr</p><p> | ||
NcoI-7 Cr</p><p> | NcoI-7 Cr</p><p> | ||
NcoI-8 Cr</p><p> | NcoI-8 Cr</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI-2</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI-5+RBS+BFP+Ter</p><p> | |
pbs3C5</p><p> | pbs3C5</p><p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter Cr</p><p> | |
| - | 9/23</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/23</span></font><br></p><p> |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 KpnI-1 Cr dig(EP)</p><p> | |
| - | + | Well 3 NdeI-1 Cr dig(EP)</p><p> | |
| - | + | Well 4 XhoI-1 Cr dig(EP)</p><p> | |
| - | + | Well 5 SacI-1 Cr dig(EP)</p><p> | |
| - | + | Well 6 AseI-1 Cr dig(EP)</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+HindIII-1 dig(EP)</p><p> | |
| - | + | Well 8 P<sub>cons</sub>+RBS +KpnI-4 dig(EP)</p><p> | |
| - | + | Well 9 P<sub>cons</sub>+RBS+NheI-4 dig(EP)</p><p> | |
| - | + | Well 10 P<sub>cons</sub>+RBS+AseI-5 dig(EP)</p><p> | |
| - | + | Well 11 P<sub>cons</sub>+RBS+XhoI-1 dig(EP)</p><p> | |
| - | + | Well 12 P<sub>cons</sub>+RBS+SacI-1 dig(EP)</p><p> | |
| - | + | Well 13 P<sub>cons</sub>+RBS+KpnI-7+RBS+BFP+Ter dig(EP)</p><p> | |
| - | + | Well 14 SalI Kr dig(EP) 9/21</p><p> | |
| - | + | Well 15 SalI Kr dig(EP) 9/19</p><p> | |
| - | DNA sequencing of | + | <font color="yellow">DNA sequencing of</font></p><p> |
KpnI-1 Cr dig(EP)</p><p> | KpnI-1 Cr dig(EP)</p><p> | ||
NdeI-1 Cr dig(EP)</p><p> | NdeI-1 Cr dig(EP)</p><p> | ||
| Line 571: | Line 573: | ||
SacI-1 Cr dig(EP)</p><p> | SacI-1 Cr dig(EP)</p><p> | ||
AseI-1 Cr dig(EP)</p><p> | AseI-1 Cr dig(EP)</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+HindIII-1 dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-4 dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI-4 dig(EP)(fail)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI-5 dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI-1 dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-1 dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+KpnI-7+RBS+BFP+Ter dig(EP)</p><p> | |
| - | Transformation of | + | <font color="yellow">Transformation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter</p><p> | |
| - | 9/24</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/24</span></font><br></p><p> |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Cr</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Cr </p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Cr </p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Cr </p><p> | |
NheI Cr </p><p> | NheI Cr </p><p> | ||
psb3C5 Cr </p><p> | psb3C5 Cr </p><p> | ||
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter Cr </p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter Cr </p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter Cr </p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter Cr </p><p> | |
NheI Cr </p><p> | NheI Cr </p><p> | ||
psb3C5 Cr </p><p> | psb3C5 Cr </p><p> | ||
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 SalI Kr dig(EP)</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+XhoI+RBS+BFP+Ter Cr dig(EP)</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+SacI Cr dig(EP)</p><p> | |
| - | + | Well 5 NcoI Cr dig(EP)</p><p> | |
| - | Digestion of | + | <font color="yellow">Digestion of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+XhoI+RBS+BFP+Ter Cr dig(EP)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-2 Cr dig(EP)</p><p> | |
NcoI-8 Cr dig(EP)</p><p> | NcoI-8 Cr dig(EP)</p><p> | ||
SalI-3 Kr dig(EP)</p><p> | SalI-3 Kr dig(EP)</p><p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
HindIII+psb1C3</p><p> | HindIII+psb1C3</p><p> | ||
| - | PCR of | + | <font color="yellow">PCR of</font></p><p> |
SacI x8</p><p> | SacI x8</p><p> | ||
SalI x5</p><p> | SalI x5</p><p> | ||
HindIII x2</p><p> | HindIII x2</p><p> | ||
NheI x8</p><p> | NheI x8</p><p> | ||
| - | Mini prep and digestion of | + | <font color="yellow">Mini-prep and digestion of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter </p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
psb3C5 </p><p> | psb3C5 </p><p> | ||
NheI</p><p> | NheI</p><p> | ||
| - | 9/25</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/25</span></font><br></p><p> |
| - | Electrophoresis and | + | <font color="yellow">Electrophoresis and DNA sequencing of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 NheI (fail)</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+AseI+RBS+BFP+Ter</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter (fail)</p><p> | |
| - | Transformation of | + | <font color="yellow">Transformation of</font></p><p> |
HindIII</p><p> | HindIII</p><p> | ||
SalI</p><p> | SalI</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | PCR of | + | <font color="yellow">PCR of</font></p><p> |
NcoI x5(9.10.11.12.13)</p><p> | NcoI x5(9.10.11.12.13)</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI x5(7.8.9.10.11)</p><p> | |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
NheI</p><p> | NheI</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI-2(9/22)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI-8(9/22)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI-4</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter x2</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter x2</p><p> | |
| - | 9/26</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/26</span></font><br></p><p> |
| - | Mini prep and digestion(EP) of | + | <font color="yellow">Mini-prep and digestion(EP) of</font></p><p> |
NcoI</p><p> | NcoI</p><p> | ||
NheI</p><p> | NheI</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SacI-2</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI-4</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI-1+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI-2+RBS+BFP+Ter</p><p> | |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+NdeI-1+RBS+BFP+Ter</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+NdeI-2+RBS+BFP+Ter</p><p> | |
| - | + | Well 4 NcoI</p><p> | |
| - | + | Well 5 NheI</p><p> | |
| - | + | Well 6 P<sub>cons</sub>+RBS+NheI-4</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+SacI-2</p><p> | |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
psb3C5 x2</p><p> | psb3C5 x2</p><p> | ||
HindIII-3</p><p> | HindIII-3</p><p> | ||
| Line 672: | Line 674: | ||
SalI-3</p><p> | SalI-3</p><p> | ||
SalI-4</p><p> | SalI-4</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+HindIII-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+HindIII-4+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI-4+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-4+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI-4+RBS+BFP+Ter</p> | |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 9/28</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/28</span></font><br></p><p> |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 HindIII-3</p><p> | |
| - | + | Well 2 HindIII-4</p><p> | |
| - | + | Well 3 SalI-3</p><p> | |
| - | + | Well 4 SalI-4</p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+SacI-3+RBS+BFP+Ter</p><p> | |
| - | + | Well 6 Marker</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+NcoI-3+RBS+BFP+Ter</p><p> | |
| - | + | Well 8 P<sub>cons</sub>+RBS+NcoI-4+RBS+BFP+Ter</p><p> | |
| - | + | Well 9 P<sub>cons</sub>+RBS+SalI-3+RBS+BFP+Ter</p><p> | |
| - | + | Well 10 P<sub>cons</sub>+RBS+SalI-4+RBS+BFP+Ter</p><p> | |
| - | + | Well 11 psb3C5</p><p> | |
| - | 9/29</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/29</span></font><br></p><p> |
| - | Electrophoresis | + | <font color="yellow">Electrophoresis of</font></p><p> |
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | Well 6 P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter</p><p> | |
| - | + | Well 8 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 9 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 10 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 11 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 12 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 13 P<sub>cons</sub>+RBS+NcoI+RBS+BFP+Ter</p><p> | |
| - | + | Well 14 P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | Well 15 P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | Well 16 P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | Well 17 P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | Well 18 P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | Well 19 P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter</p><p> | |
| - | + | Well 20 psb3C5 dig</p><p> | |
| - | + | Well 21 Marker</p><p> | |
| - | Ligation of | + | <font color="yellow">Ligation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+KpnI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+AseI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+HindIII + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI + pbs3C5</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI+RBS+BFP+Ter + pbs1C3</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter + pbs1C3</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI + pbs1C3</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NcoI + pbs1C3</p><p> | |
| - | DNA sequencing of | + | <font color="yellow">DNA sequencing of</font></p><p> |
SalI-4</p><p> | SalI-4</p><p> | ||
HindIII-4</p><p> | HindIII-4</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+SalI-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI-2</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI-4</p><p> | |
NheI(9/26)</p><p> | NheI(9/26)</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+HindIII+RBS+BFP+Ter(9/30)</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SalI+RBS+BFP+Ter(9/30)</p> | |
<p> | <p> | ||
| - | 9/30</p><p> | + | <font color="#FF0000"><span style="font-size:1cm;">9/30</span></font><br></p><p> |
| - | PCR and Electrophoresis | + | <font color="yellow">PCR and Electrophoresis of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+NdeI+RBS+BFP+Ter</p><p> | |
NcoI</p><p> | NcoI</p><p> | ||
| - | + | P<sub>cons</sub>+RBS+NheI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI+RBS+BFP+Ter</p><p> | |
| - | Cultivation of | + | <font color="yellow">Cultivation of</font></p><p> |
| - | + | P<sub>cons</sub>+RBS+NheI-11+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NheI-13+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+NdeI-10+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI-3+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+XhoI-5+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-2+RBS+BFP+Ter</p><p> | |
| - | + | P<sub>cons</sub>+RBS+SacI-11+RBS+BFP+Ter</p><p> | |
NcoI-3</p><p> | NcoI-3</p><p> | ||
NcoI-12</p> | NcoI-12</p> | ||
Latest revision as of 15:22, 17 October 2014
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9/4
Cultivation of
Pcons+RBS+AseI PCR product-1,2,3,4,7,8 Ar
XhoI+PSB1K3 PCR product-1,2 Kr
PSB1A3 Ar
Cultivation plate test
Ar plate and Kr plate
Digestion of
[PBAN-HindIII] Kr*2 (EP)
[PBAN-NheI]Kr*2 (EP)
[PBAN-KpnI-HF]Kr*2 (EP)
[23101+B0034+PBAN (HindIII)]Ar(ES)
[23101+B0034+PBAN (NheI)]Ar(ES)
PCR of
[J23101+B0034+KpnI-HF] Ar*3
Ligation of
[J23101+B0034+KpnI-HF+PSB1C3]
Electrophoresis of
[PBAN-HindIII] Kr*2 (EP)
[PBAN-NheI]Kr*2 (EP)
[PBAN-KpnI-HF]Kr*2 (EP)
[23101+B0034+PBAN (HindIII)]Ar(ES)
[23101+B0034+PBAN (NheI)]Ar(ES)
[23101+B0034+PBAN (HindIII)]Ar(EP)*2
[23101+B0034+PBAN (NheI)]Ar(EP)
[23101+B0034+PBAN (KpnI-HF)]Ar(EP)*2
[23101+B0034+PBAN (KpnI-HF)]Ar PCR*3
9/5
Electrophoresis of
Well 1 Pcons+RBS+AseI--1 (EP) Ar
Well 2 Pcons+RBS+AseI--2 (EP) Ar
Well 3 Pcons+RBS+AseI--3 (EP) Ar
Well 4 Pcons+RBS+AseI--4 (EP) Ar
Well 5 Pcons+RBS+AseI--7 (EP) Ar （DNA sequencing）
Well 6 Pcons+RBS+AseI--8 (EP) Ar
Well 7 Pcons+RBS+NcoI--2 (EP) Cr （DNA sequencing）
Well 8 Pcons+RBS+Ndel--2 (EP) Cr （DNA sequencing）
Well 9 1 Kb marker
Well 10 Pcons+RBS+Ndel--13 (EP) Cr
Well 11 SacI--3 (XP) Kr
Well 12 SacI--4.1 (XP) Kr
Well 13 SacI--4.2 (XP) Kr
Well 14 XhoI--7 (EP) Kr （DNA sequencing）
Well 15 XhoI--8 (EP) Kr（DNA sequencing）
Well 16 SalI--3 (XP) Kr
Mini-prep of
Pcons+RBS+KpnI-HF-7
Pcons+RBS+KpnI-HF-8
Pcons+RBS+KpnI-HF-9
Digestion of
Pcons+RBS+KpnI-HF-7 (EP)
Pcons+RBS+KpnI-HF-8 (EP)
Pcons+RBS+KpnI-HF-9 (EP)
Cultivation of
Pcons+RBS+KpnI-HF Ar PCR product-7,8,9 Ar
Ligation of
[J23101+B0034+HindIII+B0034+PSB1K3] Kr
[J23101+B0034+HindIII+J61048+PSB1K3] Kr
[J23101+B0034+NheI+B0034+PSB1K3] Kr
[J23101+B0034+NheI+J61048+PSB1K3] Kr
Electrophoresis of digested product of
[Pcons+RBS+KpnI-HF]Ar (EP)*3
9/9
Cultivation of
Pcons+RBS+SacI PCR product-16 Ar
Pcons+RBS+SaII PCR product-17 Ar
RBS+BFP+Ter PCR product-16,17,29,30 Ar
Digestion of
XhoI+PSB1K3 (EP)
9/10
Mini-prep of
RBS+BFP+Ter-16 Ar
RBS+BFP+Ter-17 Ar
RBS+BFP+Ter-29 Ar
RBS+BFP+Ter-30 Ar
Pcons+RBS+Sac-16 Ar
Pcons+RBS+Sal-17 Ar
Digestion of
RBS+BFP+Ter-16 Ar(EP)
RBS+BFP+Ter-17 Ar(EP)
RBS+BFP+Ter-29 Ar(EP)
RBS+BFP+Ter-30 Ar(EP)
Pcons+RBS+SacI-16 Ar(EP)
Pcons+RBS+SalI-17 Ar(EP)
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+SacI-16 dig(EP)
Well 3 Pcons+RBS+SalI-17 dig(EP)
Well 4 RBS+BFP+Ter-16 dig(EP)
Well 5 RBS+BFP+Ter-17 dig(EP)
Well 6 RBS+BFP+Ter-29 dig(EP)
Well 7 RBS+BFP+Ter-30 dig(EP)
Well 8 XhoI-7 dig(XP) Kr
9/11
Digestion of
Pcons+RBS+PBAN(ES)(8 kind except of XhoI)
RBS+BFP+Ter(XP)
Ligation of
Pcons+RBS+XhoI-7 Ar
Cultivation of
Pcons+RBS+AseI-7 Ar
Pcons+RBS+SacI-16 Ar
Pcons+RBS+SalI-17 Ar
Pcons+RBS+NcoI-2 Ar
Pcons+RBS+NdeI-2 Ar
RBS+BFP+Ter-2 Ar
RBS+BFP+Ter-2 Ar
SalI-3 Kr
Ligation of
Pcons+RBS+XhoI-7 Ar
DNA sequencing of
Pcons+RBS+SaeI Ar 8ul VF2
Pcons+RBS+SalI Ar 8ul VF2
RBS+BFP+Ter-29 Ar 8ul VF2 + 8ul VR
RBS+BFP+Ter-30 Ar 5ul VF2 + 5ul VR
Digestion of
Pcons+RBS+HindIII dig(EP)
Pcons+RBS+NheI dig(EP)
Pcons+RBS+NcoI dig(EP)-->9/11 mini
Pcons+RBS+NdeI dig(EP)
Pcons+RBS+SacI dig(EP)
Pcons+RBS+SalI dig(EP)
Pcons+RBS+AseI dig(EP)
Pcons+RBS+KpnI dig(EP)
Ligation of
Pcons+RBS+BFP+Ter-30 Kr
9/12
Transformation of
Pcons+RBS+NheI+RBS+BFP+Ter Kr
Pcons+RBS+NdeI+RBS+BFP+Ter Kr
Pcons+RBS+HindIII+RBS+BFP+Ter Kr
Pcons+RBS+KpnI-HF+RBS+BFP+Ter Kr
Pcons+RBS+AseI+RBS+BFP+Ter Kr
Pcons+RBS+SacI+RBS+BFP+Ter Kr
Pcons+RBS+NcoI+RBS+BFP+Ter Kr
Pcons+RBS+SalI+RBS+BFP+Ter Kr
Pcons+RBS+BFP+Ter Kr
Pcons+RBS+XhoI Ar
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+NdeI dig(EP) Cr
Well 3 Pcons+RBS+NcoI dig(EP) Cr
Well 4 Pcons+RBS+SacI dig(EP) Ar
Well 5 Pcons+RBS+SalI dig(EP) Ar
Well 6 Pcons+RBS+AseI dig(EP) Ar
Well 7 Pcons+RBS+HindIII dig(EP) Ar
Well 8 Pcons+RBS+NheI dig(EP) Ar
Well 9 Pcons+RBS+KpnI dig(EP) Ar
9/13
Ligation of
Pcons+RBS+AseI Kr
Pcons+RBS+NdeI Kr
Pcons+RBS+SalI Kr
Pcons+RBS+SacI Kr
PCR of
Pcons+RBS+HindIII+RBS+BFP+Ter Kr x8
Pcons+RBS+NheI+RBS+BFP+Ter Kr x8
Pcons+RBS+NdeI+RBS+BFP+Ter Kr x8
Pcons+RBS+NcoI+RBS+BFP+Ter Kr x8
Pcons+RBS+KpnI-HF+RBS+BFP+Ter Kr x8
Pcons+RBS+AseI+RBS+BFP+Ter Kr x8
Pcons+RBS+Xhol Kr x8
Pcons+RBS+SalI+RBS+BFP+Ter Kr x16
Pcons+RBS+SacI+RBS+BFP+Ter Kr x16
Electrophoresis of
Gel 1
Pcons+RBS+SalI+RBS+BFP+Ter Kr x16
Gel 2
Pcons+RBS+SacI+RBS+BFP+Ter Kr x16
Gel 3
Pcons+RBS+HindIII+RBS+BFP+Ter Kr x8
Pcons+RBS+NheI+RBS+BFP+Ter Kr x8
Gel 4
Pcons+RBS+NdeI+RBS+BFP+Ter Kr x8
Pcons+RBS+NcoI+RBS+BFP+Ter Kr x8
Gel 5
Pcons+RBS+KpnI-HF+RBS+BFP+Ter Kr x8
Pcons+RBS+AseI+RBS+BFP+Ter Kr x8
Gel 6
Pcons+RBS+Xhol Kr x8
psb1K3 dig(EP)
9/16
Transformation of
psb3K3 *2
psb3C3
Digestion of
psb3K3 dig(EP)
psb1C3 dig(EP)
Cultivation of
Pcons+RBS+PBAN+RBS+BFP+Ter x8
9/17
Mini-prep of
PBAN：XhoI
SalI
SacI
KpnI
Ndel
AseI
NcoI
NheI
HindIII (fail)
Pcons+RBS+PBAN+RBS+BFP+Ter：AseI-4
Ndel-8
HindIII-4 (fail)
SacI-9(fail)
KpnI-1
NcoI-2
NheI-1
Digestion of
PBAN：XhoI
SalI
SacI
KpnI
Ndel
AseI
NcoI
AseI
Pcons+RBS+PBAN+RBS+BFP+Ter：AseI-4
Ndel-8
KpnI-1
NcoI-2
NheI-1
Electrophoresis of
Pcons+RBS+PBAN+RBS+BFP+Ter (Ndel)
Pcons+RBS+PBAN+RBS+BFP+Ter (NcoI)
Pcons+RBS+PBAN+RBS+BFP+Ter (SacI)
Pcons+RBS+PBAN+RBS+BFP+Ter (HindIII)
Pcons+RBS+PBAN+RBS+BFP+Ter (SalI)
Pcons+RBS+PBAN+RBS+BFP+Ter (NheI)
Pcons+RBS+PBAN+RBS+BFP+Ter (KpnI)
Pcons+RBS+PBAN+RBS+BFP+Ter (AseI)
PBAN AseI
PBAN Ndel
PBAN KpnI
PBAN SacI
PBAN SalI
PBAN XhoI
Pcons+RBS+XhoI-1 dig(EP)
Pcons+RBS+Xhol-1 dig(ES)
psb1C3 dig(ES)
Cultivation of
Pcons+RBS+PBAN+RBS+BFP+Ter (SacI)-4.12
Pcons+RBS+PBAN+RBS+BFP+Ter (HindIII)-6
Pcons+RBS+PBAN+RBS+BFP+Ter (SalI)-9
Digestion of
B0034 Kr dig(ES)
Pcons+RBS+AseI+RBS+BFP+Ter Kr dig(EP)
Pcons+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)
NcoI Kr dig(EP)
Ndel Kr dig(EP)
SalI Kr dig(EP)
9/18
Electrophoresis of
Well 1 Marker
Well 2 B0034 Kr dig(ES)
Well 3 Pcons+RBS+AseI+RBS+BFP+Ter Kr dig(EP)
Well 4 Pcons+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)
Well 5 NcoI Kr dig(EP)
Well 6 Ndel Kr dig(EP)
Well 7 SalI Kr dig(EP)
Electrophoresis of
Well 1 Marker
Well 2 B0034 Kr dig(ES)
Well 3 Pcons+RBS+AseI+RBS+BFP+Ter Kr dig(EP)
Well 4 Pcons+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)
Well 5 NcoI Kr dig(EP)
Well 6 Ndel Kr dig(EP)
Well 7 SalI Kr dig(EP)
Mini-prep of
Pcons+RBS+HindIII+RBS+BFP+Ter-6
Pcons+RBS+SalI+RBS+BFP+Ter-9
Pcons+RBS+SacI+RBS+BFP+Ter-4
Pcons+RBS+SacI+RBS+BFP+Ter-11
Ligation of
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Pcons+RBS+NdeI+RBS+BFP+Ter Cr
NheI Cr
NcoI Cr
9/20
Transformation of
AseI mini Cr
Electrophoresis of
Well 1 Marker
Well 2 HindIII-2 Kr dig(EP)
Well 3 SacI Kr dig(EP)
Well 4 Pcons+RBS+HindIII-6+RBS+BFP+Ter Kr dig(EP)
Well 5 Pcons+RBS+SacI-11+RBS+BFP+Ter Kr dig(EP)
Well 6 Pcons+RBS+SacI-9+RBS+BFP+Ter Kr dig(EP)
Well 7 RBS+BFP+Ter Ar dig(XP)
Well 8 Pcons+RBS+NheI+RBS+BFP+Ter Kr dig(EP)
9/21
Electrophoresis of PCR products of
Gel 1
Well 1 Marker
Well 2 3H1
Well 3 3H2
Well 4 3H3
Well 5 3H4
Well 6 3H5
Well 7 3H6
Well 8 Marker
Well 9 6K1
Well 10 6K2
Well 11 6K3
Well 12 6K4
Well 13 6K5
Well 14 6K6
Well 15 3Sl1
Well 16 3Sl2
Well 17 3Sl3
Gel 2
Well 1 Marker
Well 2 6Sl4
Well 3 6Sl5
Well 4 3Nh1
Well 5 3Nh2
Well 6 3Nh3
Well 7 3Nh4
Well 8 Marker
Well 9 3Nh5
Well 10 3Nh6
Well 11 6X1
Well 12 6X2
Well 13 6X3
Well 14 6X4
Well 15 6X5
Well 16 6X6
Well 17 1K1
Gel 3
Well 1 Marker
Well 2 1K2
Well 3 1K3
Well 4 1K4
Well 5 1K5
Well 6 1K6
Well 7 1X1
Well 8 Marker
Well 9 1X2
Well 10 1X3
Well 11 1X4
Well 12 1X5
Well 13 1X6
Well 14 3K1
Well 15 3K2
Well 16 3K3
Well 17 3K4
Gel 4
Well 1 Marker
Well 2 3K5
Well 3 3K6
Well 4 3Sc1
Well 5 3Sc2
Well 6 3Sc3
Well 7 3Sc4
Well 8 Marker
Well 9 3Sc5
Well 10 3Sc6
Well 11 1Sc1
Well 12 1Sc2
Well 13 1Sc3
Well 14 1Sc4
Well 15 1Sc5
Well 16 1Sc6
Well 17 1Nc1
Gel 5
Well 1 Marker
Well 2 1Nc2
Well 3 1Nc3
Well 4 1Nc4
Well 5 1Nc5
Well 6 1Nc6
Well 7 1A1
Well 8 Marker
Well 9 1A2
Well 10 1A3
Well 11 1A4
Well 12 1A5
Well 13 1A6
Well 14 1Nd1
Well 15 1Nd2
Well 16 1Nd3
Well 17 1Nd4
Gel 6
Well 1 Marker
Well 2 1Nd5
Well 3 1Nd6
Well 4 3X1
Well 5 3X2
Well 6 3X3
Well 7 3X4
Well 8 Marker
Well 9 3X5
Well 10 3X6
Well 11 3A1
Well 12 3A2
Well 13 3A3
Well 14 3A4
Well 15 3A5
Well 16 3A6
Cultivation of
KpnI-1
NdeI-1
XhoI-1
SacI-1
AseI-1
Pcons+RBS+HindIII-1
Pcons+RBS+KpnI-4
Pcons+RBS+NheI-4
Pcons+RBS+SalI-5
Pcons+RBS+AseI-5
Pcons+RBS+XhoI-1
Pcons+RBS+SacI-1
Pcons+RBS+KpnI-1+RBS+BFP+Ter
Pcons+RBS+XhoI-6+RBS+BFP+Ter
Digestion of
SalI Kr dig(EP)
Ligation of
NdeI dig(EP)+C backbone
9/22
Transformation of
NheI Cr
Pcons+RBS+NheI+RBS+BFP+Ter Cr
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+Ndel+RBS+BFP+Ter Cr
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Digestion of
HindIII(EP)
Cultivation of
SacI-3 Kr
NcoI-7 Cr
NcoI-8 Cr
Pcons+RBS+SacI-2
Pcons+RBS+XhoI-5+RBS+BFP+Ter
pbs3C5
Ligation of
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+HindIII+RBS+BFP+Ter Cr
Pcons+RBS+SacI+RBS+BFP+Ter Cr
Pcons+RBS+SalI+RBS+BFP+Ter Cr
9/23
Electrophoresis of
Well 1 Marker
Well 2 KpnI-1 Cr dig(EP)
Well 3 NdeI-1 Cr dig(EP)
Well 4 XhoI-1 Cr dig(EP)
Well 5 SacI-1 Cr dig(EP)
Well 6 AseI-1 Cr dig(EP)
Well 7 Pcons+RBS+HindIII-1 dig(EP)
Well 8 Pcons+RBS +KpnI-4 dig(EP)
Well 9 Pcons+RBS+NheI-4 dig(EP)
Well 10 Pcons+RBS+AseI-5 dig(EP)
Well 11 Pcons+RBS+XhoI-1 dig(EP)
Well 12 Pcons+RBS+SacI-1 dig(EP)
Well 13 Pcons+RBS+KpnI-7+RBS+BFP+Ter dig(EP)
Well 14 SalI Kr dig(EP) 9/21
Well 15 SalI Kr dig(EP) 9/19
DNA sequencing of
KpnI-1 Cr dig(EP)
NdeI-1 Cr dig(EP)
XhoI-1 Cr dig(EP)
SacI-1 Cr dig(EP)
AseI-1 Cr dig(EP)
Pcons+RBS+HindIII-1 dig(EP)
Pcons+RBS+KpnI-4 dig(EP)
Pcons+RBS+NheI-4 dig(EP)(fail)
Pcons+RBS+AseI-5 dig(EP)
Pcons+RBS+XhoI-1 dig(EP)
Pcons+RBS+SacI-1 dig(EP)
Pcons+RBS+KpnI-7+RBS+BFP+Ter dig(EP)
Transformation of
Pcons+RBS+HindIII+RBS+BFP+Ter
Pcons+RBS+NcoI+RBS+BFP+Ter
Pcons+RBS+SalI+RBS+BFP+Ter
Pcons+RBS+SacI+RBS+BFP+Ter
9/24
Cultivation of
Pcons+RBS+NheI+RBS+BFP+Ter Cr
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+NdeI+RBS+BFP+Ter Cr
NheI Cr
psb3C5 Cr
Cultivation of
Pcons+RBS+NheI+RBS+BFP+Ter Cr
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+NdeI+RBS+BFP+Ter Cr
NheI Cr
psb3C5 Cr
Electrophoresis of
Well 1 Marker
Well 2 SalI Kr dig(EP)
Well 3 Pcons+RBS+XhoI+RBS+BFP+Ter Cr dig(EP)
Well 4 Pcons+RBS+SacI Cr dig(EP)
Well 5 NcoI Cr dig(EP)
Digestion of
Pcons+RBS+XhoI+RBS+BFP+Ter Cr dig(EP)
Pcons+RBS+SacI-2 Cr dig(EP)
NcoI-8 Cr dig(EP)
SalI-3 Kr dig(EP)
Ligation of
HindIII+psb1C3
PCR of
SacI x8
SalI x5
HindIII x2
NheI x8
Mini-prep and digestion of
Pcons+RBS+AseI+RBS+BFP+Ter
Pcons+RBS+NheI+RBS+BFP+Ter
Pcons+RBS+NdeI+RBS+BFP+Ter
Pcons+RBS+NcoI+RBS+BFP+Ter
psb3C5
NheI
9/25
Electrophoresis and DNA sequencing of
Well 1 Marker
Well 2 NheI (fail)
Well 3 Pcons+RBS+AseI+RBS+BFP+Ter
Well 4 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 5 Pcons+RBS+NdeI+RBS+BFP+Ter (fail)
Transformation of
HindIII
SalI
Pcons+RBS+SacI+RBS+BFP+Ter
Pcons+RBS+SalI+RBS+BFP+Ter
Pcons+RBS+HindIII+RBS+BFP+Ter
PCR of
NcoI x5(9.10.11.12.13)
Pcons+RBS+SacI x5(7.8.9.10.11)
Cultivation of
NheI
Pcons+RBS+SacI-2(9/22)
Pcons+RBS+NcoI-8(9/22)
Pcons+RBS+NheI-4
Pcons+RBS+NdeI+RBS+BFP+Ter x2
Pcons+RBS+NheI+RBS+BFP+Ter x2
9/26
Mini-prep and digestion(EP) of
NcoI
NheI
Pcons+RBS+SacI-2
Pcons+RBS+NheI-4
Pcons+RBS+NdeI-1+RBS+BFP+Ter
Pcons+RBS+NdeI-2+RBS+BFP+Ter
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+NdeI-1+RBS+BFP+Ter
Well 3 Pcons+RBS+NdeI-2+RBS+BFP+Ter
Well 4 NcoI
Well 5 NheI
Well 6 Pcons+RBS+NheI-4
Well 7 Pcons+RBS+SacI-2
Cultivation of
psb3C5 x2
HindIII-3
HindIII-4
SalI-3
SalI-4
Pcons+RBS+HindIII-3+RBS+BFP+Ter
Pcons+RBS+HindIII-4+RBS+BFP+Ter
Pcons+RBS+SalI-3+RBS+BFP+Ter
Pcons+RBS+SalI-4+RBS+BFP+Ter
Pcons+RBS+SacI-3+RBS+BFP+Ter
Pcons+RBS+SacI-4+RBS+BFP+Ter
Pcons+RBS+NcoI-3+RBS+BFP+Ter
Pcons+RBS+NcoI-4+RBS+BFP+Ter
9/28
Electrophoresis of
Well 1 HindIII-3
Well 2 HindIII-4
Well 3 SalI-3
Well 4 SalI-4
Well 5 Pcons+RBS+SacI-3+RBS+BFP+Ter
Well 6 Marker
Well 7 Pcons+RBS+NcoI-3+RBS+BFP+Ter
Well 8 Pcons+RBS+NcoI-4+RBS+BFP+Ter
Well 9 Pcons+RBS+SalI-3+RBS+BFP+Ter
Well 10 Pcons+RBS+SalI-4+RBS+BFP+Ter
Well 11 psb3C5
9/29
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 3 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 4 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 5 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 6 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 7 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 8 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 9 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 10 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 11 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 12 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 13 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 14 Pcons+RBS+SalI+RBS+BFP+Ter
Well 15 Pcons+RBS+SalI+RBS+BFP+Ter
Well 16 Pcons+RBS+SalI+RBS+BFP+Ter
Well 17 Pcons+RBS+SalI+RBS+BFP+Ter
Well 18 Pcons+RBS+SalI+RBS+BFP+Ter
Well 19 Pcons+RBS+SalI+RBS+BFP+Ter
Well 20 psb3C5 dig
Well 21 Marker
Ligation of
Pcons+RBS+KpnI + pbs3C5
Pcons+RBS+SacI + pbs3C5
Pcons+RBS+AseI + pbs3C5
Pcons+RBS+HindIII + pbs3C5
Pcons+RBS+NheI + pbs3C5
Pcons+RBS+SalI + pbs3C5
Pcons+RBS+NcoI + pbs3C5
Pcons+RBS+NdeI + pbs3C5
Pcons+RBS+XhoI+RBS+BFP+Ter + pbs1C3
Pcons+RBS+NdeI+RBS+BFP+Ter + pbs1C3
Pcons+RBS+SacI + pbs1C3
Pcons+RBS+NcoI + pbs1C3
DNA sequencing of
SalI-4
HindIII-4
Pcons+RBS+SalI-3+RBS+BFP+Ter
Pcons+RBS+SalI-2
Pcons+RBS+NheI-4
NheI(9/26)
Pcons+RBS+HindIII+RBS+BFP+Ter(9/30)
Pcons+RBS+SalI+RBS+BFP+Ter(9/30)
9/30
PCR and Electrophoresis of
Pcons+RBS+NdeI+RBS+BFP+Ter
NcoI
Pcons+RBS+NheI+RBS+BFP+Ter
Pcons+RBS+XhoI+RBS+BFP+Ter
Pcons+RBS+SacI+RBS+BFP+Ter
Cultivation of
Pcons+RBS+NheI-11+RBS+BFP+Ter
Pcons+RBS+NheI-13+RBS+BFP+Ter
Pcons+RBS+NdeI-3+RBS+BFP+Ter
Pcons+RBS+NdeI-10+RBS+BFP+Ter
Pcons+RBS+XhoI-3+RBS+BFP+Ter
Pcons+RBS+XhoI-5+RBS+BFP+Ter
Pcons+RBS+SacI-2+RBS+BFP+Ter
Pcons+RBS+SacI-11+RBS+BFP+Ter
NcoI-3
NcoI-12
