Team:INSA-Lyon/Parts

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<div id="igem"><a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/2/23/Insa_igem.png" alt="IGEM" /></a>
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<h4>PARTS</h4>
<h4>PARTS</h4>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Biology" class="hu-icon"><li class="iconmulti">WETLAB SUMMARY</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Results" class="hu-icon"><li class="icon">RESULTS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Notebook" class="hu-icon"><li class="icon">NOTEBOOK</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/Protocol" class="hu-icon"><li class="icon">PROTOCOLS</li></a>
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<a href="https://2014.igem.org/Team:INSA-Lyon/DataPage" class="hu-icon"><li class="icon">DATA PAGE</li></a>
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<p> Texte</p>
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<p>5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.</p></br>
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<b>Biobricks for nickel chelation</b></br></br>
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<p>The principal of the construction is shown in the figure below.</p></br></br></br></div>
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<img src= "https://static.igem.org/mediawiki/parts/6/63/1.JPG"></br>
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<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br>
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The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.</br>
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The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.</br>
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The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.</br>
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Latest revision as of 17:07, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • 5 biobricks have been constructed, thre for Nickel chelation, two for promoter characterization.


    Biobricks for nickel chelation

    The principal of the construction is shown in the figure below.





  • On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.
    The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.
    The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.
    The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.

    • <groupparts>iGEM014 INSA-Lyon</groupparts>

    Retrieved from "http://2014.igem.org/Team:INSA-Lyon/Parts"