Team:Brasil-SP/Notebook/CharacterizationAssemblies

From 2014.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
-
{{CSS/Main}}
+
{{:Team:Brasil-SP/Templates/Header}}
<html>
<html>
<head>
<head>
 +
<style>
 +
b.dashed {border-style: dashed;}
 +
</style>
 +
<style>
 +
b:hover {
 +
    border:2px; #d7ccea;
 +
    border-style: dashed;
 +
 +
}
 +
</style>
<!--main content -->
<!--main content -->
Line 23: Line 33:
<table width="70%" align="center">
<table width="70%" align="center">
   <td colspan="2" align = center>
   <td colspan="2" align = center>
 +
 +
<!-- imagemap1-->
     <style type="text/css">
     <style type="text/css">
Line 30: Line 42:
     </style>
     </style>
     <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/5/59/KI_w_2.png');">
     <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/5/59/KI_w_2.png');">
-
<a class="map_link" id="map_link_0" title="" href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>
+
<b class="map_link" id="map_link_0" title="" href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</b>
     </div>
     </div>
Line 49: Line 61:
     <p><strong>Question</strong>: How does the transcription caused by this promoter varies with the IPTG induction?</p>
     <p><strong>Question</strong>: How does the transcription caused by this promoter varies with the IPTG induction?</p>
 +
 +
<!--imagemap2-->
 +
    <style type="text/css">
 +
.map_image_b { display: block; width: 660px; height: 406px; position: relative; background-position: 0 0; background-repeat: no-repeat; }
 +
.map_image_b .map_link_b { display: block; position: absolute; text-indent: -999em; overflow: hidden; }
 +
.map_image_b #map_link_0_b { width: 301px; height: 146px; top: 20px; left: 1px; }
 +
.map_image_b #map_link_1_b { width: 280px; height: 152px; top: 16px; left: 359px; }
 +
    </style>
 +
    <div class="map_image_b" style="background-image: url('https://static.igem.org/mediawiki/2014/8/8c/KXVI_w_2.png');">
 +
<b class="map_link_b" id="map_link_0_b" title="" href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</b>
 +
<b class="map_link_b" id="map_link_1_b" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</b>
 +
    </div>
 +
</div>
</div>
Line 65: Line 90:
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
-
<!--huge figure-->
+
<!--imagemap3-->
-
 
+
<div align=center>
<div align=center>
Line 81: Line 112:
     </style>
     </style>
     <div class="map_image_c" style="background-image: url('https://static.igem.org/mediawiki/2014/3/3d/KXIII_w2.png');">
     <div class="map_image_c" style="background-image: url('https://static.igem.org/mediawiki/2014/3/3d/KXIII_w2.png');">
-
<a class="map_link_c" id="map_link_0_c" title="" href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>
+
<b class="map_link_c" id="map_link_0_c" title="" href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</b>
-
<a class="map_link_c" id="map_link_1_c" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
+
<b class="map_link_c" id="map_link_1_c" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</b>
-
<a class="map_link_c" id="map_link_2_c" title="" href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a>
+
<b class="map_link_c" id="map_link_2_c" title="" href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</b>
-
<a class="map_link_c" id="map_link_3_c" title="" href=""></a>
+
<b class="map_link_c" id="map_link_3_c" title="" href=""></b>
-
<a class="map_link_c" id="map_link_4_c" title="" href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>
+
<b class="map_link_c" id="map_link_4_c" title="" href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</b>
-
<a class="map_link_c" id="map_link_5_c" title="" href=""></a>
+
<b class="map_link_c" id="map_link_5_c" title="" href=""></b>
</div>
</div>
-
<!--
+
<br>
-
  <style type="text/css">
+
<br>
-
.map_image_a { display: block; width: 1224px; height: 764px; position: relative; background-position: 0 0; background-repeat: no-repeat; }
+
<br>
-
.map_image_a .map_link_a { display: block; position: absolute; text-indent: -999em; overflow: hidden; }
+
<br>
-
.map_image_a #map_link_0_a { width: 212px; height: 145px; top: 37px; left: 1px; }
+
<br>
-
.map_image_a #map_link_1_a { width: 608px; height: 150px; top: 35px; left: 244px; }
+
<br>
-
.map_image_a #map_link_2_a { width: 194px; height: 149px; top: 35px; left: 904px; }
+
-
.map_image_a #map_link_3_a { width: 219px; height: 143px; top: 231px; left: 939px; }
+
-
.map_image_a #map_link_4_a { width: 742px; height: 161px; top: 311px; left: 86px; }
+
-
.map_image_a #map_link_6_a { width: 882px; height: 167px; top: 579px; left: 201px; }
+
-
    </style>
+
-
    <div class="map_image_a" style="background-image: url('https://static.igem.org/mediawiki/2014/3/3d/KXIII_w2.png');">
+
-
<a class="map_link_a" id="map_link_0_a" title="" href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>
+
-
<a class="map_link_a" id="map_link_1_a" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
+
-
<a class="map_link_a" id="map_link_2_a" title="" href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a>
+
-
<a class="map_link_a" id="map_link_3_a" title="" href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>
+
-
<a class="map_link_a" id="map_link_4_a" title="" href=""></a>
+
-
<a class="map_link_a" id="map_link_6_a" title="" href=""></a>
+
-
    </div>
+
-
-->
+
<!--imagemap4-->
 +
 
 +
<style type="text/css">
 +
.map_image_d { display: block; width: 800px; height: 412px; position: relative; background-position: 0 0; background-repeat: no-repeat; }
 +
.map_image_d .map_link_d { display: block; position: absolute; text-indent: -999em; overflow: hidden; }
 +
.map_image_d #map_link_0_d { width: 137px; height: 82px; top: 19px; left: 1px; }
 +
.map_image_d #map_link_1_d { width: 399px; height: 80px; top: 19px; left: 160px; }
 +
.map_image_d #map_link_2_d { width: 131px; height: 80px; top: 18px; left: 588px; }
 +
.map_image_d #map_link_3_d { width: 483px; height: 88px; top: 167px; left: 57px; }
 +
.map_image_d #map_link_4_d { width: 143px; height: 80px; top: 124px; left: 610px; }
 +
.map_image_d #map_link_5_d { width: 575px; height: 93px; top: 311px; left: 130px; }
 +
</style>
 +
<div class="map_image_d" style="background-image: url('https://static.igem.org/mediawiki/2014/f/fa/KXIV_w2.png');">
 +
<b class="map_link_d" id="map_link_0_d" title="" href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</b>
 +
<b class="map_link_d" id="map_link_1_d" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</b>
 +
<b class="map_link_d" id="map_link_2_d" title="" href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">AIV</b>
 +
<b class="map_link_d" id="map_link_3_d" title="" href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</b>
 +
<b class="map_link_d" id="map_link_4_d" title="" href=""></b>
 +
<b class="map_link_d" id="map_link_5_d" title="" href=""></b>
 +
</div>
 +
 
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
<br>
 +
 
 +
<!--imagemap5-->
 +
 
 +
<style type="text/css">
 +
.map_image_e { display: block; width: 800px; height: 412px; position: relative; background-position: 0 0; background-repeat: no-repeat; }
 +
.map_image_e .map_link_e { display: block; position: absolute; text-indent: -999em; overflow: hidden; }
 +
.map_image_e #map_link_0_e { width: 137px; height: 82px; top: 19px; left: 1px; }
 +
.map_image_e #map_link_1_e { width: 399px; height: 80px; top: 19px; left: 160px; }
 +
.map_image_e #map_link_2_e { width: 131px; height: 80px; top: 18px; left: 588px; }
 +
.map_image_e #map_link_3_e { width: 483px; height: 88px; top: 167px; left: 57px; }
 +
.map_image_e #map_link_4_e { width: 143px; height: 80px; top: 124px; left: 610px; }
 +
.map_image_e #map_link_5_e { width: 575px; height: 93px; top: 311px; left: 130px; }
 +
</style>
 +
<div class="map_image_e" style="background-image: url('https://static.igem.org/mediawiki/2014/3/3a/KXV_w2.png');">
 +
<b class="map_link_e" id="map_link_0_e" title="" href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</b>
 +
<b class="map_link_e" id="map_link_1_e" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</b>
 +
<b class="map_link_e" id="map_link_2_e" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</b>
 +
<b class="map_link_e" id="map_link_3_e" title="" href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</b>
 +
<b class="map_link_e" id="map_link_4_e" title="" href=""></b>
 +
<b class="map_link_e" id="map_link_5_e" title="" href=""></b>
 +
</div>
<!--end huge figure-->
<!--end huge figure-->
</html>
</html>
 +
 +
{{:Team:Brasil-SP/Templates/Footer}}

Latest revision as of 17:03, 13 October 2014

[[Image:{{{headersrc}}}|1079px]]


Characterization Assemblies

Apart from the main genetic circuit we also assembled others for characterization purposes, such as the validation of the promoters and tunning of our threshold setter concentration, the QteE.

Promoter BBa_K823003

Question: Does the constitutive promoter BBa_K823003 work properly?

KI

Results: After the incubation period of the transformed E. coli a large portion of the colonies were glowing green. So the promoter does work. Moreover, this biobrick works on E. coli despite the fact it was designed for B. subtilis.

Promoter BBa_K143015

Question: How does the transcription caused by this promoter varies with the IPTG induction?

KV BIII

Tunning of the QteE Threshold

Question:What are the concentration of QteE needed to hamper the LasR induction of the promoter PlasR?

This is the most difficult task of our project. Tunning the production of QteE so that we establish the correct threshold for the discretization of the Cystatin C level in serum. To attack this challenge we designed 3 circuits so that we could plot a calibration curve. In this circuits we put the transcription of the LasR and QteE under two differnt promoters, the Pveg (BBa_K823003) and PlasR (BBa_K143015).







KII CII KIV KXI






KIII CII AIV KX






KIII CII AIV KX

Sponsors

This project is part of iGEM 2014

Wiki Links

Recent Changes Related Changes Special Pages Privacy Policy What Links Here Upload File My Preferences Disclaimers

Retrieved from "http://2014.igem.org/Team:Brasil-SP/Notebook/CharacterizationAssemblies"