Team:Bordeaux/Attributions

From 2014.igem.org

(Difference between revisions)
m
 
(64 intermediate revisions not shown)
Line 5: Line 5:
<p>A project is like a seed which require some elements to grow up, in order to bud <br><br>
<p>A project is like a seed which require some elements to grow up, in order to bud <br><br>
-
This is why our team required the help of others specialist in different domain.<br>
+
This is why our team required the help of others specialist in different domains.<br>
-
The work lab was done by 9 members of the Igem Bordeaux team 2014 during the summer,we was sub devised in groups and every one was responsible of a particular work.<br><br>
+
The work lab was done by 10 members of the Igem Bordeaux team 2014 during the summer, we were subdivised into groups and each one was responsible for a particular task.<br><br>
<img class="attribution" src="https://static.igem.org/mediawiki/2014/0/00/Team_Bordeaux_2014Attribution.jpg"><br><br>
<img class="attribution" src="https://static.igem.org/mediawiki/2014/0/00/Team_Bordeaux_2014Attribution.jpg"><br><br>
-
- Justine and Zohra :<br>
+
The students developed the project during several brainstorming sessions at the European Institute of Chemistry and Biology.<br>
-
- Jean Remy and Edouard :<br>
+
-
- Caroline , Laurianne and Cassandra :<br>
+
-
- Claudia , Hiba and Gilles :<br>
+
-
 
+
-
The students developed the project independently during several brainstorming sessions at the European Institute of Chimistry and Biology.<br>
+
All of the designs, constructs, and experiments presented in this Wiki were performed by the students of the team.<br>
All of the designs, constructs, and experiments presented in this Wiki were performed by the students of the team.<br>
-
The instructors and advisors were providing feedbacks and advices, when we need it.<br><br></p>
+
The instructors and advisors were providing feedbacks and advice, when it was needed.<br><br></p>
<h1>Advisors and supervisors</h1>
<h1>Advisors and supervisors</h1>
-
   <p><b>Dr. Denis DUPUY</b><br>
+
   <img src="http://www.iecb.u-bordeaux.fr/images/stories/Equipes/Dupuy/ddupuy.jpg" title="Denis Dupuy" align="left" style="width:120px; height:107px; margin:0px 100px"/>
-
Denis is team leader in U 869 Inserm who works on Genome regulation and evolution.<br>
+
<p><b>Denis DUPUY, PhD</b><br>
-
He supervise the Igem Bordeaux team for the 3rd year , help us to avoid mistakes in our experiments. He authorize the team to work in his lab wich is equipped for experiment in genetics. This is why we needed the help of other persons in the institute.<br>
+
Denis is a group leader at the European Insitute of Chemistry and Biology (U869 - Inserm) and works on post-transcriptional regulation in <i>C.elegans</i>.<br>
-
Denis worked with us to success in our project , he find the idea of the project « elasticoli » we worked together to find brainstorming idea , communication , human practices…<br></p>
+
He supervises the iGEM team of Bordeaux for the 3rd year. His involvement allowed the team to work in his lab where most of the labwork has been done.<br>
 +
At his side, we all came up with the idea of the « elasticoli » project.</p>
 +
<img src="http://i.imgur.com/xGDq4bc.png?1" align="left" title="Elisabeth Garanger" style="width:120px; height:107px; margin:5px 100px"/><br><br>
 +
 
 +
 
 +
<p><b>Elisabeth GARANGER, PhD</b><br>
 +
Elisabeth is a group leader at the European Insitute of Chemistry and Biology (LCPO - CNRS) and works on Peptide-based polymer assemblies. She was present every time to advise the team about Elastin Like Peptid synthesis and let us use different machines and solvents.<br>
 +
</p><br><img src="http://dept-info.labri.fr/~beurton/images/marie.jpg" title="Marie Beurton-Aimar" align="left" style="width:120px; height:107px; margin:5px 100px 0 100px"/>
 +
 
 +
<p><b>Marie Beurton-Aimar, PhD</b><br>
 +
Lecturer at université de Bordeaux and reasearcher at the Laboratoire Bordelais de Recherche en Informatique. She was in charge of the Bio-informatics group.<br></p><br><br>
 +
 
 +
 
 +
<h1 align="center">Host lab</h1>
 +
 
 +
<p>
 +
 
 +
  Bordeaux IGEM team was hosted at the European Institute of Chemistry and Biology(IECB), which allows many scientist to share and develop their projects. It is a multi-disciplinary institute focused on biology, chemistry & informatics.<br><br>
 +
 
 +
Several labs hosted in the IECB provided us all the machines necessary for microbiology and molecular biology such as centrifuges, thermocyclers, sterile hoods, freezers, incubators, microscopes, plate readers, glassware, spectrophotometer and so on. We were in charge of taking care of the stocks as well as ordering lab consumables such as gloves, plates, pipettes, tips, and tubes as well as chemicals and DNA.<br><br></p>
 +
 
 +
  <img src="https://static.igem.org/mediawiki/2014/0/0b/Bdx2014_B_Garbay2.jpg" title="Bertrand GARBAY" align="left" style="width:120px; height:107px; margin:0px 100px"/>
 +
<p><b>Bertrand GARBAY (EA4135) :</b><br>
 +
Professor and researcher in the University of Bordeaux, he allowed our team to manage the construction of recombinant DNA coding for the ELPs and the production of these proteins. This work is based on Bertrand GARBAY (EA4135)
 +
and Laure BATAILLE (LCPO/IECB) research.</p><br><br><br>
 +
 
 +
  <img src="http://www.iecb.u-bordeaux.fr/images/stories/Equipes/Fribourg/sfribourg.jpg" title="Sebastien Fribourg" align="left" style="width:120px; height:107px; margin:0px 100px"/>
 +
<p><b>Sebastien Fribourg’s Group :</b><br>
 +
This group works on structural biology, the group leader let us use the sonicator to lyse our cultures before centrifugation, their different centrifuges and materials for protein production were very helpful.</p><br><br><br>
 +
 
 +
  <img src="http://i.imgur.com/F1n2sbw.png?1" title="Lionel Beaurepaire" align="left" style="width:120px; height:107px; margin:0px 100px"/>
 +
<p><b>Lionel Beaurepaire :</b><br>
 +
Lionel Beaurepaire is a research engineer he advised us on the production and purification protocol, in order to get more product. he was very helpful for us, he is the boss of production proteins</p><br><br><br><br>
-
<p><b>Dr. Elisabeth GARANGER</b><br>
+
<img src="https://static.igem.org/mediawiki/2014/8/87/Bdx2014_Attributions6.jpg" title="Axel Innis" align="left" style="width:120px; height:107px; margin:10px 100px 0px 100px"/>
-
Elisabeth is a team leader in CNRS unite LCPO wich work on Peptide-based polymer assemblies. She was present every time to advice the team about Elastin Like Peptid synthesis and permit to use different machines and solvents  <br>
+
<p><b>Axel Innis group :</b><br>
-
</p>
+
The group led by Axel Innis (PhD) work on translational regulation of gene expression, Axel authorized us to use his lab equipment.</p><br><br><br><br>
 +
<img src="http://i.imgur.com/vBwUNad.png?1" title="Britta & Carolin" align="left" style="width:120px; height:107px; margin:10px 100px 0px 100px"/>
 +
<p>"The German Power" <b>Carolin SEEFELDT</b> (Graduate student) and <b>Britta SEIP</b> (Post-doc) gave us a lot of helpful advices about protein precipitation.<br>
 +
Our nighbours from the lab near to us, helped us by lending us an incubator, some tips, centrifuge and giving us some piece of advice about electrophoresis.</p><br><br><br>
-
<p><b>Dr. Marie Beurton-Aimar</b><br>
+
<img src="https://static.igem.org/mediawiki/2014/7/72/Bdx2014_Attributions10.jpg" title="Martin Teichmann" align="left" style="width:120px; height:120px; margin:10px 100px 0px 100px"/>
-
maître de conférences au Laboratoire Bordelais de Recherche en Informatique.<br></p>
+
<p><b> Martin Teichmann group:</b><br>  
 +
He is a professor at the University of and a researcher at the IECB. His work is based on Gene regulation & tumor research.</p><br><br><br><br><br><br><br>
 +
<img src="https://static.igem.org/mediawiki/2014/5/5f/Bdx2014_CRPP.png" title="CRPP" align="left" style="width:120px; height:107px; margin:10px 10px 0px 100px"/>
 +
<p><b>CRPP</b><br>
 +
For the transformation of our proteins into threads we collaborated with the Centre de Recherche Paul Pascal, which is located in the same campus as our lab.<br>
 +
We heard about their work on a TV report. We met two scientists there who helped us to elaborate a succesfull way to form our fiber.<br>
 +
The CRPP allowed to us to transform our proteins from liquid state to fiber, we characterized our product by using a machine allowing measurement of elasticity and resistance of polymers.</p><br><br><br><br>
-
<p><b>Host lab :</b><br>
+
<img src="https://static.igem.org/mediawiki/2014/e/ec/Bdx2014_Attributions8.jpg" title="Wilfrid Neri" align="left" style="width:120px; height:120px; margin:10px 100px 0px 100px"/>
 +
<p><strong>Wilfrid Neri </strong>:<br>
 +
Called “Spider Engineer” he is a chemistry Engineer (ITA CNRS) and works on production of nanocarbone fiber which could replace Kevlar one day !</p><br><br><br><br><br><br><br>
-
Bordeaux IGEM team was hosted at the European  Institute of Chimistry and Biology , which permits to many scientist to share and develope their project,there is team reasercher of different domains.<br><br>
+
<img src="https://static.igem.org/mediawiki/2014/d/d6/Bdx2014_Attributions9.jpg" title="Cecile Zakri" align="left" style="width:120px; height:120px; margin:0px 100px 0px 100px"/>
 +
<p>
 +
<strong>Cecile Zakri</strong> :<br> Professor at Bordeaux University , and researcher at CRPP on nanotubes and graphene.<br>
 +
</p><br><br><br><br><br><br>
-
Some lab hosted by IECB provided us all the machines necessary for microbiology and molecular biology such as centrifuges, PCR machines, sterile hoods, freezers, incubators, microscopes, plate readers, glassware, a NanoDrop machine, etc.. We were in charge of taking care of the stocks as well as ordering lab consumables such as gloves, plates, pipettes, tips, and tubes as well as chemicals and DNA. All the media preparation and the
+
<h1>External organisms</h1>
-
glassware washing was done by us.<br><br></p>
+
<br><br>
 +
<img  src="https://static.igem.org/mediawiki/2014/2/25/Bdx2014_Genscript_logo.jpg" align="left" style="width:200px; height:50px; margin:0px 100px 0px 100px"/>
-
<p><b>Dr Sebastien Fribourg’s Group :</b><br>
+
<p><strong>GenScript</strong>
-
The team work on structural bochimistry,the team leader permits to us to use the sonicator to lyse our bacterium before centrifugation, their different centrifuges and materials for protein production.<br>
+
GenScript sponsored us by offering us a synthesis. In parallel of our own synthesis, we asked them to synthesize two of our coding sequences (for BBa_K1317001 and BBa_K1317002).<br><br><br>
-
- Lionel Beaurepaire Research Engineer adviced us to modify the production and purification protocol , in order to get more rentability. He guided the team to improve our protein production <br></p>
+
We used these sequences to assemble the biobricks because it was sequenced, and so we could provide the registry with the proper sequence without mutations.</p><br><br><br><br>
-
<p><b>Dr. Axel Innis group :</b><br>
+
 
-
In this lab they work on Translational Regulation of Gene Expression , Axel authorized the acces to his lab for our team. We daily used nanodrop , hot centrifuge<br>
+
-
- Dr. Britta SEIP, Post-doc<br>
+
-
- Carolin SEEFELDT, Graduate student<br>
+
-
Those two scientist adviced us about the right technics to adopt to succes in our experiments, they help us to correct the way that we employed to precipit proteins.<br></p>
+
-
</p>
+
</html>
</html>
 +
{{:Team:Bordeaux/Pied}}

Latest revision as of 00:40, 18 October 2014

A project is like a seed which require some elements to grow up, in order to bud

This is why our team required the help of others specialist in different domains.
The work lab was done by 10 members of the Igem Bordeaux team 2014 during the summer, we were subdivised into groups and each one was responsible for a particular task.



The students developed the project during several brainstorming sessions at the European Institute of Chemistry and Biology.
All of the designs, constructs, and experiments presented in this Wiki were performed by the students of the team.
The instructors and advisors were providing feedbacks and advice, when it was needed.

Advisors and supervisors

Denis DUPUY, PhD
Denis is a group leader at the European Insitute of Chemistry and Biology (U869 - Inserm) and works on post-transcriptional regulation in C.elegans.
He supervises the iGEM team of Bordeaux for the 3rd year. His involvement allowed the team to work in his lab where most of the labwork has been done.
At his side, we all came up with the idea of the « elasticoli » project.



Elisabeth GARANGER, PhD
Elisabeth is a group leader at the European Insitute of Chemistry and Biology (LCPO - CNRS) and works on Peptide-based polymer assemblies. She was present every time to advise the team about Elastin Like Peptid synthesis and let us use different machines and solvents.


Marie Beurton-Aimar, PhD
Lecturer at université de Bordeaux and reasearcher at the Laboratoire Bordelais de Recherche en Informatique. She was in charge of the Bio-informatics group.



Host lab

Bordeaux IGEM team was hosted at the European Institute of Chemistry and Biology(IECB), which allows many scientist to share and develop their projects. It is a multi-disciplinary institute focused on biology, chemistry & informatics.

Several labs hosted in the IECB provided us all the machines necessary for microbiology and molecular biology such as centrifuges, thermocyclers, sterile hoods, freezers, incubators, microscopes, plate readers, glassware, spectrophotometer and so on. We were in charge of taking care of the stocks as well as ordering lab consumables such as gloves, plates, pipettes, tips, and tubes as well as chemicals and DNA.

Bertrand GARBAY (EA4135) :
Professor and researcher in the University of Bordeaux, he allowed our team to manage the construction of recombinant DNA coding for the ELPs and the production of these proteins. This work is based on Bertrand GARBAY (EA4135) and Laure BATAILLE (LCPO/IECB) research.




Sebastien Fribourg’s Group :
This group works on structural biology, the group leader let us use the sonicator to lyse our cultures before centrifugation, their different centrifuges and materials for protein production were very helpful.




Lionel Beaurepaire :
Lionel Beaurepaire is a research engineer he advised us on the production and purification protocol, in order to get more product. he was very helpful for us, he is the boss of production proteins





Axel Innis group :
The group led by Axel Innis (PhD) work on translational regulation of gene expression, Axel authorized us to use his lab equipment.





"The German Power" Carolin SEEFELDT (Graduate student) and Britta SEIP (Post-doc) gave us a lot of helpful advices about protein precipitation.
Our nighbours from the lab near to us, helped us by lending us an incubator, some tips, centrifuge and giving us some piece of advice about electrophoresis.




Martin Teichmann group:
He is a professor at the University of and a researcher at the IECB. His work is based on Gene regulation & tumor research.








CRPP
For the transformation of our proteins into threads we collaborated with the Centre de Recherche Paul Pascal, which is located in the same campus as our lab.
We heard about their work on a TV report. We met two scientists there who helped us to elaborate a succesfull way to form our fiber.
The CRPP allowed to us to transform our proteins from liquid state to fiber, we characterized our product by using a machine allowing measurement of elasticity and resistance of polymers.





Wilfrid Neri :
Called “Spider Engineer” he is a chemistry Engineer (ITA CNRS) and works on production of nanocarbone fiber which could replace Kevlar one day !








Cecile Zakri :
Professor at Bordeaux University , and researcher at CRPP on nanotubes and graphene.







External organisms



GenScript GenScript sponsored us by offering us a synthesis. In parallel of our own synthesis, we asked them to synthesize two of our coding sequences (for BBa_K1317001 and BBa_K1317002).


We used these sequences to assemble the biobricks because it was sequenced, and so we could provide the registry with the proper sequence without mutations.