Team:HIT-Harbin/Project

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        <li><a href="https://2014.igem.org/Team:HIT-Harbin/Abstract">Abstract</a></li>
 
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Background">Background</a></li>
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Background">Background</a></li>
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Design">Design</a></li>
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        <li><a href="https://2014.igem.org/Team:HIT-Harbin/Advantages">Advantages</a></li>
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Modeling">Modeling</a></li>
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         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Notebook">Notebook</a></li>
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         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Attribution">Attribution</a></li>
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Attribution">Attribution</a></li>
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Acknowledge">Acknowledge</a></li>
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         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Humanpractices#Course">Course</a></li>
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        <li><a href="https://2014.igem.org/Team:HIT-Harbin/Background">Background</a></li>
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        <li><a href="https://2014.igem.org/Team:HIT-Harbin/Design">Design</a></li>
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        <li><a href="https://2014.igem.org/Team:HIT-Harbin/FutureWork">Advantages</a></li>
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        <li><a href="https://2014.igem.org/Team:HIT-Harbin/Modeling">Modeling</a></li>
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<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="Overview: " style="width: 59px; height: 18px;"><cufontext>Overview </cufontext></cufon></span></h5>
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<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="Overview: " style="width: 59px; height: 18px;"><cufontext>Abstract </cufontext></cufon></span></h5>
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                     <p>Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.</p>
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                     <p> How to detect dioxins among the environment rapidly, with high-end HRGC-MS, or CALUX? This year, HITGEM brings you the new bio-machine – Dioxin Detective Y. For the letter Y, it means yeast. But how can the bacteria which have been manipulated for thousands of years before, detect the new poison which did not emerge until post-industrial era in a rapid way?</p>
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  <p>In the gene pool of mouse, there exists sequence, say, mouse dioxin receptor 83-805 (mDR83-805), that can express the receptor of dioxin-Heat shock protein (hsp90). We can obtain the receptor hsp90 by modifying this sequence and transform it into yeasts. This dual domain protein can pass through the karyotheca and combine with DNA only after unite with dioxin. After the combination with DNA, it will activate the expression of the downstream sequences. Together with positive feedback, this device can detect the small amount of dioxin in the environment.
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<p>So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings.
</p>
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      <h2>Reference:</h2>
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<p>1.Mimura J, Fujii-Kuriyama Y. Functional role of AhR in the expression of toxic effects by TCDD[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2003, 1619(3): 263-268.
 +
</p>
 +
<p>2.Denison M S, Heath-Pagliuso S. The Ah receptor: a regulator of the biochemical and toxicological actions of structurally diverse chemicals[J]. Bulletin of environmental contamination and toxicology, 1998, 61(5): 557-568.
 +
</p>
 +
<p>3.Toren A, Segal G, Ron E Z, et al. Structure–function studies of the recombinant protein bioemulsifier AlnA[J]. Environmental microbiology, 2002, 4(5): 257-261.
 +
</p>
 +
<p>4.Furukawa K, Fujihara H. Microbial degradation of polychlorinated biphenyls: biochemical and molecular features[J]. Journal of bioscience and bioengineering, 2008, 105(5): 433-449.
 +
</p>
 +
<p>5.Whitelaw M L, Göttlicher M, Gustafsson J A, et al. Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor[J]. The EMBO journal, 1993, 12(11): 4169.</p>
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                     <a href="https://2014.igem.org/Team:HIT-Harbin/Modeling" class="custom-button">Learn More</a>
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<h2>SPONSORS</h2>
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<p></p>
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<a href="http://en.hit.edu.cn/">
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<a href="http://food.hit.edu.cn/">
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</a>
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Latest revision as of 01:47, 18 October 2014

Project

Abstract

Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.

So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings.

Reference:

1.Mimura J, Fujii-Kuriyama Y. Functional role of AhR in the expression of toxic effects by TCDD[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2003, 1619(3): 263-268.

2.Denison M S, Heath-Pagliuso S. The Ah receptor: a regulator of the biochemical and toxicological actions of structurally diverse chemicals[J]. Bulletin of environmental contamination and toxicology, 1998, 61(5): 557-568.

3.Toren A, Segal G, Ron E Z, et al. Structure–function studies of the recombinant protein bioemulsifier AlnA[J]. Environmental microbiology, 2002, 4(5): 257-261.

4.Furukawa K, Fujihara H. Microbial degradation of polychlorinated biphenyls: biochemical and molecular features[J]. Journal of bioscience and bioengineering, 2008, 105(5): 433-449.

5.Whitelaw M L, Göttlicher M, Gustafsson J A, et al. Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor[J]. The EMBO journal, 1993, 12(11): 4169.

Learn More

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SPONSORS