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Team:Hannover/Protocols/EasyIsolation
From 2014.igem.org
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<h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / A simple method for DNA isolation</h1> | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / A simple method for DNA isolation</h1> | ||
<p class="text">A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.</p> | <p class="text">A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.</p> | ||
| - | <table style="float:right"><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/a/ad/Hannover_20141003_Sbd6_klein.jpg | + | <table style="float:right" style="spadtable"><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/a/ad/Hannover_20141003_Sbd6_klein.jpg |
" data-lightbox="galery" data-title="Our DNA-Isolation kit"><img src="https://static.igem.org/mediawiki/2014/thumb/a/ad/Hannover_20141003_Sbd6_klein.jpg/120px-Hannover_20141003_Sbd6_klein.jpg" width="160px" style="center"></a></td></tr><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/c/ce/Hannover_20141003_Sbd3.jpg | " data-lightbox="galery" data-title="Our DNA-Isolation kit"><img src="https://static.igem.org/mediawiki/2014/thumb/a/ad/Hannover_20141003_Sbd6_klein.jpg/120px-Hannover_20141003_Sbd6_klein.jpg" width="160px" style="center"></a></td></tr><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/c/ce/Hannover_20141003_Sbd3.jpg | ||
" data-lightbox="galery" data-title="DNA-Isolation"><img src="https://static.igem.org/mediawiki/2014/thumb/c/ce/Hannover_20141003_Sbd3.jpg/120px-Hannover_20141003_Sbd3.jpg | " data-lightbox="galery" data-title="DNA-Isolation"><img src="https://static.igem.org/mediawiki/2014/thumb/c/ce/Hannover_20141003_Sbd3.jpg/120px-Hannover_20141003_Sbd3.jpg | ||
" width="140px" style="center"></a></td></tr> | " width="140px" style="center"></a></td></tr> | ||
</table> | </table> | ||
| - | <table border="0"> | + | <table border="0" class="spadtable"> |
<tr><td><h4>Material:</h4></td> | <tr><td><h4>Material:</h4></td> | ||
<tr><td>Bananas</td><td>Tap water</td> | <tr><td>Bananas</td><td>Tap water</td> | ||
Latest revision as of 07:01, 15 October 2014
Protocols / A simple method for DNA isolation
A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.
 |
 |
Material: |
|
| Bananas | Tap water |
| Table salt | Dishwashing liquid as detergent |
| Washing agent with proteases | Hot water (60 °C) |
| Ice | Teaspoon |
| Tablespoon | Fork |
| Isopropyl (alcohol) (cooled) | Coffee filter |
| Funnel | 50 mL vessels |
Test execution:
- preparation of extraction solution:
- 1 teaspoon table salt
- 1 tablespoon dishwashing liquid
- 10 drops washing agent
- Fill up to 100 mL with tap water
- Mixing by shaking
- Homogenization of a banana with the fork
- Transfer of 3 teaspoons homogenized banana into a 50mL reaction vessel with 30 mL extraction solution.
- 15 min incubation at 60 °C
- 5 min chilling on ice
- Filter the mixture into a new 50 mL vessel.
- Overlay the filtered solution with some iosporyl (alcohol)
- Wait for 10 min
- DNA is soaring into the phase of isopropyl (alcohol)
