|
|
| (17 intermediate revisions not shown) |
| Line 6: |
Line 6: |
| | | | |
| | | | |
| - | <div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;min-width:645px;"> | + | <div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;min-width:300px;"> |
| - | <img src="https://static.igem.org/mediawiki/2014/2/2b/Oxigemred.jpg" style="position:absolute; width:100%;z-index:-1; border-radius:15px;"/> | + | <img src="https://static.igem.org/mediawiki/2014/0/08/Oxigemradcamcrop.jpg" style="position:absolute; width:100%;z-index:-1; border-radius:15px;"/> |
| | | | |
| | <div style="background-color:#D9D9D9; opacity:0.7; z-index:5; Height:75px; width:100%;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;margin-top:10px;"> | | <div style="background-color:#D9D9D9; opacity:0.7; z-index:5; Height:75px; width:100%;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;margin-top:10px;"> |
| - | <div style="background-color:white; opacity:0.7; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#00000; font-weight: 450;"><br><center><font style="opacity:0.7">Results</font></center></div> | + | <div style="background-color:white; opacity:0.7; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#00000; font-weight: 450;"><br><center><font style="opacity:0.7">Interlab Results</font></center></div> |
| | </div> | | </div> |
| | <br> | | <br> |
| Line 17: |
Line 17: |
| | | | |
| | | | |
| - | <img src="https://static.igem.org/mediawiki/2014/8/89/OxigemBronze.png" style="width:26%;margin-left:9%;margin-top:-20px;">
| |
| - | <img src="https://static.igem.org/mediawiki/2014/8/8e/OxigemGold.png" style="width:30%;margin-top:-20px;">
| |
| - | <img src="https://static.igem.org/mediawiki/2014/1/1e/OxigemSilver.png" style="width:26%;margin-top:-20px;">
| |
| | | | |
| | | | |
| | </div> | | </div> |
| - | <div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-35px;"> | + | <div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:100px;"> |
| - | <br><br> | + | <br> |
| - | agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy agsdhg dhgsdkf sdfaskjdf hkjasdfhua duf asudoas dou saod sud vouas vouasod os oas oas dos au uf sfus fus fuyis fi sif yf iy
| + | |
| | + | <center style="font-size:20px;font-weight:600;">View our interlab results pdf <a href="https://static.igem.org/mediawiki/2014/8/86/Oxigem_Interlab_results.pdf" target="_blank">here!</a></center> |
| | + | <div class=row><br> |
| | + | |
| | + | We have given each measurement here in arbitrary units - as provided by our laboratory's plate-reader and spectrophotometer.<br><br>Adjusted fluorescence is calculated as the mean fluo of each biological replicate minus the mean fluo for the DH5-a, giving an approximation for the fluorescence due to the devices only.<br><br>Cell counts were estimated based the OD, using the online calculator given by the link: www.genomics.agilent.com%2fbiocalculators%2fcalcODBacterial.jsp%3f_requestid%3d826255 <br><br> Results were given in triplicate with respect to biological replicates (meaning three separate colonies were taken from the same plates); and duplicate with respect to the technical replicates (two 200ul samples were taken from each culture to be read).<br><br>Although it is clear from visual inspection that there are significant differences in fluorescence depending on device, the fluorescence of each sample can be modelled as the following function:<br><br><br>FLUO = DEVICE + AUTO(CELLS) + AUTO(MEDIA) + ERROR(BIOLOGICAL) + ERROR(TECHNICAL).</div> |
| | | | |
| | <br> | | <br> |
"
