Team:Aberdeen Scotland/Project

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<a href="https://2014.igem.org/Team:Aberdeen_Scotland"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Aberdeen_Scotland/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Aberdeen_Scotland"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Aberdeen_Scotland/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Aberdeen_Scotland/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Aberdeen_Scotland/Attributions"style="color:#000000"> Attributions </a></td>
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<tr><td > <h3> Project Description </h3></td>
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<p>Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) </p>
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<a href="http://abdn.ac.uk" title="University of Aberdeen"></a>
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>  
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland">Home</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Modeling">Modelling</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Notebook">Notebook</a></li>
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<li class="curr"><a class="curr" href="https://2014.igem.org/Team:Aberdeen_Scotland/Project">Overview</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Project/Disease">The Disease</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Project/Methods">Current Methods</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Project/Design">Our Design</a></li>
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<li><a href="https://2014.igem.org/Team:Aberdeen_Scotland/Project/Assay">Assay</a></li>
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<h1><i>E. coli</i>-based Trypanosomiasis Diagnostic System</h1>
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<h3>Getting back at the Sleeping Sickness by detecting it early</h3>
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It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.  
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<p><i>E.coli </i> display of peptide mimotopes, integrated with quorum-sensing AND logic, for diagnosis of tropical diseases
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<i>Trypanosoma.b.gambiense</i>, the causative agent of Trypanosomiasis (African Sleeping Sickness) infects 30,000 people worldwide, with 60 million at disease risk.
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Unrecognised, this disease is fatal, but accurate diagnosis, relying on detection of patient serum antibodies against two different trypanosomal antigens, allows
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successful treatment. We engineered autotransporters Antigen 43 and ice-nucleation protein to successfully express surface epitopes in <i>E.coli</i> that mimic two different
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trypanosomal antigens. Two <i>E.coli</i> strains were generated, each expressing a distinct surface epitope and either a quorum sensing (QS) sender or receiver module
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respectively. Immune pull-down of each strain, and their subsequent co-culture, produced a quorum signal, indicating successful detection of two distinct antibody
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populations in a serum sample. We constructed a cheap, Raspberry Pi computer-controlled fluorimeter suitable for developing countries, showing it could detect QS
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fluorescence output. This novel application of antigen display, and quorum sensing to implement AND logic sensing, facilitates cheap, simple diagnosis of tropical
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diseases.</p>
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<li><a href="http://www.abdn.ac.uk/" title="University of Aberdeen"><img src="https://static.igem.org/mediawiki/2014/c/c6/Uni_of_ab.png"></a></li>
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<li><a href="http://www.bbsrc.ac.uk/" title="BBSRC"><img src="https://static.igem.org/mediawiki/2014/a/a9/Bbsrc.png"></a></li>
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<li><a href="http://www.biochemistry.org" title="Biochemical Society"><img src="https://static.igem.org/mediawiki/2014/7/7c/Biochem_soc.png"></a></li>
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<li><a href="http://www.sgm.ac.uk/" title="Society for General Microbiology"><img src="https://static.igem.org/mediawiki/2014/f/f5/Soc_general_microb.png"></a></li>
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<li><a href="http://www.sulsa.ac.uk/" title="SULSA"><img src="https://static.igem.org/mediawiki/2014/2/24/Sulsa.png"></a></li>
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Latest revision as of 02:33, 18 October 2014

Team:Aberdeen Scotland/Project - 2014.ogem.org



E. coli-based Trypanosomiasis Diagnostic System

Getting back at the Sleeping Sickness by detecting it early


E.coli display of peptide mimotopes, integrated with quorum-sensing AND logic, for diagnosis of tropical diseases Trypanosoma.b.gambiense, the causative agent of Trypanosomiasis (African Sleeping Sickness) infects 30,000 people worldwide, with 60 million at disease risk. Unrecognised, this disease is fatal, but accurate diagnosis, relying on detection of patient serum antibodies against two different trypanosomal antigens, allows successful treatment. We engineered autotransporters Antigen 43 and ice-nucleation protein to successfully express surface epitopes in E.coli that mimic two different trypanosomal antigens. Two E.coli strains were generated, each expressing a distinct surface epitope and either a quorum sensing (QS) sender or receiver module respectively. Immune pull-down of each strain, and their subsequent co-culture, produced a quorum signal, indicating successful detection of two distinct antibody populations in a serum sample. We constructed a cheap, Raspberry Pi computer-controlled fluorimeter suitable for developing countries, showing it could detect QS fluorescence output. This novel application of antigen display, and quorum sensing to implement AND logic sensing, facilitates cheap, simple diagnosis of tropical diseases.




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