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| - | <div class="site-description"><h4><span class="colored"><cufon class="cufon cufon-canvas" alt="Parts" style="width: 54px; height: 20px;"><canvas width="60" height="27" style="width: 60px; height: 27px; top: -7px; left: 1px;"></canvas><cufontext>Parts</cufontext></cufon></span></h4></div>
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| - | <p>Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote </p>
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| - | <h5 class="hashed"><span>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</span></h5>
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| - | <img src="https://static.igem.org/mediawiki/2013/f/f8/Hrps_sequence_cai_before_optimization.png">
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| - | <p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p>
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| - | <p></p>CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%
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| - | <div align="center">
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| - | <img src="https://static.igem.org/mediawiki/2013/d/d3/Hrps_sequence_cai_after_optimization.png">
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| - | <p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p>
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| - | </div>
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| - | <p></p>CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%
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| - | <img src="https://static.igem.org/mediawiki/2013/a/a8/Hrpr_sequence_cai_before_optimization.png">
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| - | <p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p>
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| - | </div>
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| - | <p></p>CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70
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| - | <img src="https://static.igem.org/mediawiki/2013/8/8b/Hrpr_sequence_cai_after_optimization.png">
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| - | <p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p>
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| - | </div>
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| - | <p></p>CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%
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| - | <h4>reference</h4>
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| - | <p></p>[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33
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