Team:HIT-Harbin/Judging

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                    <div class="site-description"><h4><span class="colored"><cufon class="cufon cufon-canvas" alt="Parts" style="width: 54px; height: 20px;"><canvas width="60" height="27" style="width: 60px; height: 27px; top: -7px; left: 1px;"></canvas><cufontext>Parts</cufontext></cufon></span></h4></div>
 
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<p>Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by  Andreas Grote </p>
 
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<h5 class="hashed"><span>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</span></h5>
 
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<img src="https://static.igem.org/mediawiki/2013/f/f8/Hrps_sequence_cai_before_optimization.png">
 
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<p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p>
 
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<p></p>CAI-Value of the sequence before optimization:0.2736.    GC-Content of the sequence before optimization:59.17%
 
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<img src="https://static.igem.org/mediawiki/2013/d/d3/Hrps_sequence_cai_after_optimization.png">
 
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<p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p>
 
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<p></p>CAI-Value of the optimized sequence:0.9832    GC-Content of the optimized sequence:53.60%
 
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<img src="https://static.igem.org/mediawiki/2013/a/a8/Hrpr_sequence_cai_before_optimization.png">
 
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<p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p>
 
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<p></p>CAI-Value of the sequence before optimization:0.2490.    GC-Content of the sequence before optimization:57.70
 
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<img src="https://static.igem.org/mediawiki/2013/8/8b/Hrpr_sequence_cai_after_optimization.png">
 
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<p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p>
 
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<p></p>CAI-Value of the optimized sequence:0.9721.    GC-Content of the optimized sequence:53.69%
 
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<h4>reference</h4>
 
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<p></p>[1] Andreas Grote et al.  JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33
 
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Latest revision as of 18:46, 17 October 2014