|
|
| (8 intermediate revisions not shown) |
| Line 9: |
Line 9: |
| | <tr style="background-color:#CCD6EA; "><td colspan="2"> | | <tr style="background-color:#CCD6EA; "><td colspan="2"> |
| | <p class="title"><font color="#002B9B"> | | <p class="title"><font color="#002B9B"> |
| - | Conclusion | + | $Conclusion$ |
| | </font></p> | | </font></p> |
| | </td></tr> | | </td></tr> |
| | </table> | | </table> |
| | </br> | | </br> |
| - | <!-- back + text -->
| |
| | | | |
| | + | <section style="margin: -40px"></section> |
| | <section style="text-align: justify; margin: 50px"> | | <section style="text-align: justify; margin: 50px"> |
| - | <p>
| |
| - | The population dynamics modelling showed first that the impact of the antibiotics must be included in a realistic model, because without antibiotics, E.Coli bacteria would finish without the plasmids necessary to activate our Mighty Coli system $\small\&$ secondly that the bacterial population grows at a particulary high rate in the beginning, but rapidly it converges until a constant quantity. Consequently, it is important to add bacterial food enough into the LB medium and to select the more productive bacteria, f.e. using a bioreator containing initially non-productive bacteria and where Mighty Coli could work.</p>
| |
| - |
| |
| - | The TA system modelling consisted to evaluate in which circumstances (sets of kinetic parameters) Mighty Coli is useful after estimating the degree of cooperativity in the system where antitoxin (gene situated in a plasmid constanlty maintained generation by generation, with a promoter not influenced by the medium) is unstable in comparison with the toxin (gene situated in another plasmid with a promoter activated or repressed by the medium), but is necessary for E.coli to survive. A Michaelis-Menten statistics was chosen and the equations were solved at stationary state to obtain analytical expression between the parameters (and depending of the PI to produce, but also of the kind of bioreactor).</p>
| |
| - |
| |
| - | The p2A modelling: estimating the cleavage rate of the 2A peptid chosen, the frequency of the mutations and the ratio [Tox]/[Anti-Tox], we obtained the boost of the production in a bioreactor using Mighty Coli (with a Toxin gene situated in the bacterial chromosome of E.coli, annihilated by an Anti-Tox gene activation with a plasmid maintained in the medium) in comparison with using the classical Stabi system, for in vivo protein production.</p>
| |
| - |
| |
| - |
| |
| - | A future continuation of our project could be to study accurately the 2A peptides to refine our current model and to build new Biobricks using different 2A peptids, ready to be applied in bioreactors.</p>
| |
| - | </section>
| |
| | | | |
| | </tr> | | </tr> |
| - | <font color="white">
| + | <!-- Important remark about this section of the Website: |
| - | <!-- -Tant qu'il n'y a pas de comparaison avec le labo, pas de géniale conclusion... --> | + | détails about TA-system and 2A modelling were not written in the pages |
| - | Coming Soon ^
| + | of this ex-Website, because of the absence of motivation in modelling |
| - | </font>
| + | of some people. Please contact the Modelling staff to obtain the models |
| | + | if you are interested in. --> |
| | | | |
| | <!-- Previous and Next pages --> | | <!-- Previous and Next pages --> |
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}}
\newcommand{\Stabi}{\small Stabi}$
$\newcommand{\EColi}{\small E.coli}
\newcommand{\SCere}{\small S.cerevisae}\\[0cm]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\PI}{\small PI}$
$\newcommand{\Igo}{\Large\mathcal{I}}
\newcommand{\Tgo}{\Large\mathcal{T}}
\newcommand{\Ogo}{\Large\mathcal{O}}
~$
"
- Université Libre de Bruxelles -
