Team:Michigan

From 2014.igem.org

(Difference between revisions)
 
(48 intermediate revisions not shown)
Line 25: Line 25:
<div id = "header-container" class = "container">
<div id = "header-container" class = "container">
-
<div id = "logo">
+
<div id = "logo" style="width:150px;height:150px">
 +
 
<a id = "site-title" href = "index.html">
<a id = "site-title" href = "index.html">
-
<img src = "http://che.engin.umich.edu/enewsletter/winter2013/images/MSBTlogo2.jpg" alt = "MSBT" />
+
<img src = "https://static.igem.org/mediawiki/2014/6/61/Umichsigil.jpg" alt = "MSBT" style="width:110px;height:110px;position:absolute;top:-76px;left:1px" />
</a><!--- #site-title -->
</a><!--- #site-title -->
<span id = "site-description">
<span id = "site-description">
-
2014 MSBT
 
</span><!--- #site-description -->
</span><!--- #site-description -->
</div><!--- #logo -->
</div><!--- #logo -->
Line 38: Line 38:
<ul>
<ul>
<li class = "nav-menu-item current">
<li class = "nav-menu-item current">
-
<a href = "index.html">Home</a>
+
<a href = "https://2014.igem.org/Team:Michigan">Home</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "elements.html">Project</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Project/">Project</a>
<ul class = "sub-menu">
<ul class = "sub-menu">
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "elements.html">Overview</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Project/">Background</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Background</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Design/">Design</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Human Practice</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Modeling/">Modeling</a>
</li>
</li>
-
 
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Outreach</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Results/">Results</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Wet Lab</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Future/">Future Plans</a>
-
</li>
+
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Dry Lab</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Accomplishments/">Accomplishments</a>
</li>
</li>
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "columns.html">Future Plans</a>
 
-
</li>
 
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "#">Pages</a>
 
-
<ul class = "sub-menu">
 
-
<li class = "nav-menu-item">
 
-
<a href = "about.html">About Us</a>
 
-
</li>
 
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "404.html">Error 404</a>
 
-
</li>
 
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "faq.html">FAQ</a>
 
-
</li>
 
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "services.html">Services</a>
 
-
</li>
 
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "contact.html">Contact Us</a>
 
-
</li>
 
-
</ul>
 
-
</li>
 
</ul>
</ul>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "portfolio_filterable.html">Human Practice</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Human/">Human Practice</a>
<ul class = "sub-menu">
<ul class = "sub-menu">
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "#">Classic Portfolio</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Outreach/">Outreach</a>
-
<ul class = "sub-menu">
+
-
<li class = "nav-menu-item">
+
-
<a href = "portfolio_classic_1_column.html">Portfolio 1 Column</a>
+
-
</li>
+
-
+
-
<li class = "nav-menu-item">
+
-
<a href = "portfolio_classic_2_columns.html">Portfolio 2 Columns</a>
+
-
</li>
+
-
+
-
<li class = "nav-menu-item">
+
-
<a href = "portfolio_classic_3_columns.html">Portfolio 3 Columns</a>
+
-
</li>
+
-
+
-
<li class = "nav-menu-item">
+
-
<a href = "portfolio_classic_4_columns.html">Portfolio 4 Columns</a>
+
-
</li>
+
-
</ul>
+
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "#">Compact Portfolio</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Economic/">Economic Analysis</a>
-
<ul class = "sub-menu">
+
-
<li class = "nav-menu-item">
+
-
<a href = "portfolio_compact_2_columns.html">Portfolio 2 Columns</a>
+
-
</li>
+
-
+
-
<li class = "nav-menu-item">
+
-
<a href = "portfolio_compact_3_columns.html">Portfolio 3 Columns</a>
+
-
</li>
+
-
</ul>
+
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "portfolio_filterable.html">Filterable Portfolio</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Covance/">Covance Partnership</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "portfolio_single.html">Single Project</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Collaborations/">Collaborations</a>
</li>
</li>
 +
 +
</ul>
</ul>
</li>
</li>
-
+
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "blog.html">Notebook</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Notebook/">Notebook</a>
 +
</li>
 +
 
 +
<li class = "nav-menu-item">
 +
<a href = "https://2014.igem.org/Team:Michigan/Team/">Team</a>
<ul class = "sub-menu">
<ul class = "sub-menu">
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "blog.html">Blog Listing</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Team/"> Team Members </a>
</li>
</li>
-
 
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "blog_single.html">Single Post</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Sponsors"> Sponsors </a>
</li>
</li>
-
</ul>
 
-
</li>
 
-
 
-
<li class = "nav-menu-item">
 
-
<a href = "contact.html">Team</a>
 
</li>
</li>
 +
</ul>
</ul>
</nav><!--- #main-nav-menu -->
</nav><!--- #main-nav-menu -->
Line 172: Line 118:
</div><!--- #header-container -->
</div><!--- #header-container -->
</header><!--- #header -->
</header><!--- #header -->
-
+
<!--- <div style="background-color:#f1ed6f;width:994px;height:120px;position:absolute;left:-5px;top:51px"></div> -->
Line 179: Line 125:
<header id = "page-header" class = "center">,
<header id = "page-header" class = "center">,
-
<h1><font size="10" <em class = "colored">MSBT</em></font></h1></p><font size="3"><font color="#191970"> the University of Michigan iGEM Team </font></font color></p>
+
<h1><font size="10"> MSBT</font></h1></p><font size="3"><font color="#191970"> University of Michigan iGEM Team </font></font color></p>
 +
 
</header><!--- #page-header -->
</header><!--- #page-header -->
-
+
-
<div id = "page-content">
+
<div style="height:400px;width:450px;position:relative;top:-7px"> <img src="https://static.igem.org/mediawiki/2014/4/46/2website_front.png" style="width:450px;height:400px;position:relative;left:213px"> </div>
-
+
<div style="width:1000px"></div>
-
<div class = "has-divider">
+
-
<div class = "flexslider">
+
-
<ul class = "slides">
+
-
<li><img alt = "Image" src = "assets/home_slides/responsive.jpg" /></li>
+
-
<li><img alt = "Image" src = "assets/home_slides/html5.jpg" /></li>
+
-
<li><img alt = "Image" src = "assets/home_slides/awesome.jpg" /></li>
+
-
</ul>
+
-
</div>
+
</div>
</div>
-
+
<p style="position:relative;width:903px;top:-14px;left:39px"> <font color="#888888"> Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in <I>E. coli</I> because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing <i>Salmonella</i>, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities. </font>
-
<div class = "portfolio portfolio-4 row has-divider">
+
</p>
-
<div class = "span-3">
+
<p style="position:absolute;top:566px;left:38px"><font size="6"> <u>Abstract</u></font></p>
-
<h3 class = "fancy-title">Featured Work</h3>
+
-
+
-
<p>
+
-
Etiam auctor tincidunt augue at pharetra. Nam posuere tristique sem, eu ultricies tortor imperdiet vitae.
+
-
</p>
+
-
+
-
<a href = "#">View Our Portfolio &mdash;</a>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<article class = "entry">
+
-
<div class = "entry-wrap">
+
-
<div class = "entry-feature">
+
-
<a href = "assets/full/1.jpg" class = "overlay colorbox" title = "Mexican Coke">
+
-
<span class = "overlay-color"></span>
+
-
<span class = "overlay-icon zoom"></span>
+
-
<img alt = "Image" src = "assets/resized_s/1.jpg" />
+
-
</a>
+
-
</div><!-- .entry-feature -->
+
-
+
-
<div class = "entry-body">
+
-
<header>
+
-
<h2 class = "entry-title">
+
-
<a href = "#">Mexican Coke</a>
+
-
</h2><!-- .entry-title -->
+
-
+
-
<div class = "entry-meta">
+
-
<span class = "tags">
+
-
<span>Art Direction</span>
+
-
<span class = "separator">|</span>
+
-
<span>Photography</span>
+
-
</span>
+
-
</div><!-- .entry-meta -->
+
-
</header>
+
-
</div><!-- .entry-body -->
+
-
</div><!-- .entry-wrap -->
+
-
</article>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<article class = "entry">
+
-
<div class = "entry-wrap">
+
-
<div class = "entry-feature">
+
-
<a href = "assets/full/2h.jpg" class = "overlay colorbox" title = "The Land Within">
+
-
<span class = "overlay-color"></span>
+
-
<span class = "overlay-icon zoom"></span>
+
-
<img alt = "Image" src = "assets/resized_s/2h.jpg" />
+
-
</a>
+
-
</div><!-- .entry-feature -->
+
-
+
-
<div class = "entry-body">
+
-
<header>
+
-
<h2 class = "entry-title">
+
-
<a href = "#">The Land Within</a>
+
-
</h2><!-- .entry-title -->
+
-
+
-
<div class = "entry-meta">
+
-
<span class = "tags">
+
-
<span>Art Direction</span>
+
-
<span class = "separator">|</span>
+
-
<span>Illustration</span>
+
-
</span>
+
-
</div><!-- .entry-meta -->
+
-
</header>
+
-
</div><!-- .entry-body -->
+
-
</div><!-- .entry-wrap -->
+
-
</article>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<article class = "entry">
+
-
<div class = "entry-wrap">
+
-
<div class = "entry-feature">
+
-
<a href = "assets/full/6.jpg" class = "overlay colorbox" title = "Apples 2 Apples">
+
-
<span class = "overlay-color"></span>
+
-
<span class = "overlay-icon zoom"></span>
+
-
<img alt = "Image" src = "assets/resized_s/6.jpg" />
+
-
</a>
+
-
</div><!-- .entry-feature -->
+
-
+
-
<div class = "entry-body">
+
-
<header>
+
-
<h2 class = "entry-title">
+
-
<a href = "#">Apples 2 Apples</a>
+
-
</h2><!-- .entry-title -->
+
-
+
-
<div class = "entry-meta">
+
-
<span class = "tags">
+
-
<span>Digital Art</span>
+
-
<span class = "separator">|</span>
+
-
<span>Illustration</span>
+
-
</span>
+
-
</div><!-- .entry-meta -->
+
-
</header>
+
-
</div><!-- .entry-body -->
+
-
</div><!-- .entry-wrap -->
+
-
</article>
+
-
</div>
+
-
</div>
+
-
+
-
<div class = "row has-divider clients-grid">
+
-
<div class = "span-3">
+
-
<h3 class = "fancy-title">Our Clients</h3>
+
-
+
-
<p>
+
-
Sed tristique aliquet massa a vulputate. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia.
+
-
</p>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<a href = "#" class = "entry overlay">
+
-
<span class = "overlay-color"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2011/thumb/7/76/Michigan_logo2.png/300px-Michigan_logo2.png" />
+
-
</a>
+
-
+
-
<a href = "#" class = "entry overlay">
+
-
<span class = "overlay-color"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2011/thumb/7/76/Michigan_logo2.png/300px-Michigan_logo2.png" />
+
-
</a>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<a href = "#" class = "entry overlay">
+
-
<span class = "overlay-color"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2011/thumb/7/76/Michigan_logo2.png/300px-Michigan_logo2.png" />
+
-
</a>
+
-
+
-
<a href = "#" class = "entry overlay">
+
-
<span class = "overlay-color"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2011/thumb/7/76/Michigan_logo2.png/300px-Michigan_logo2.png" />
+
-
</a>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<a href = "#" class = "entry overlay">
+
-
<span class = "overlay-color"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2011/thumb/7/76/Michigan_logo2.png/300px-Michigan_logo2.png" />
+
-
</a>
+
-
+
-
<a href = "#" class = "entry overlay">
+
-
<span class = "overlay-color"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2011/thumb/7/76/Michigan_logo2.png/300px-Michigan_logo2.png" />
+
-
</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class = "row has-divider">
+
-
<div class = "span-3" style = "text-align: center;">
+
-
<img alt = "Image" src = "assets/services/brush-icon.png" />
+
-
+
-
<h4>Web Design</h4>
+
-
<p>
+
-
Nulla sit amet iaculis consectetur. Pellentesque dolor, lobortis at rhoncus a, feugiat quis dolor. Pellentesque mi lorem, lacinia fermentum quis.
+
-
</p>
+
-
</div>
+
-
+
-
<div class = "span-3" style = "text-align: center;">
+
-
<img alt = "Image" src = "assets/services/scissors-icon.png" />
+
-
+
-
<h4>Simplification</h4>
+
-
<p>
+
-
Nulla sit amet iaculis consectetur. Pellentesque dolor, lobortis at rhoncus a, feugiat quis dolor. Pellentesque mi lorem, lacinia fermentum quis.
+
-
</p>
+
-
</div>
+
-
+
-
<div class = "span-3" style = "text-align: center;">
+
-
<img alt = "Image" src = "assets/services/cloud-icon.png" />
+
-
+
-
<h4>Marketing</h4>
+
-
<p>
+
-
Nulla sit amet iaculis consectetur. Pellentesque dolor, lobortis at rhoncus a, feugiat quis dolor. Pellentesque mi lorem, lacinia fermentum quis.
+
-
</p>
+
-
</div>
+
-
+
-
<div class = "span-3" style = "text-align: center;">
+
-
<img alt = "Image" src = "assets/services/leaf-icon.png" />
+
-
+
-
<h4>Branding</h4>
+
-
<p>
+
-
Nulla sit amet iaculis consectetur. Pellentesque dolor, lobortis at rhoncus a, feugiat quis dolor. Pellentesque mi lorem, lacinia fermentum quis.
+
-
</p>
+
-
</div>
+
-
</div>
+
-
+
-
<div class = "tagline has-divider">
+
-
<h2>Proin ornare, nec varius, augue sodales massa, in commodo nisi diam a lacus?</h2>
+
-
+
-
<p>
+
-
Nulla sit amet iaculis consectetur. Pellentesque dolor, lobortis at rhoncus a, feugiat quis dolor. Pellentesque mi lorem, lacinia fermentum quis.
+
-
</p>
+
-
+
-
<button class = "medium" >Let's work together!</button>
+
-
</div>
+
-
+
</div><!--- #page-content -->
</div><!--- #page-content -->
</div><!--- #page-container -->
</div><!--- #page-container -->
-
</section><!--- #page -->
+
</section><!--- #page -->
-
+
-
+
-
+
-
<footer id = "page-footer" class = "container-wrap">
+
-
<div id = "page-footer-container" class = "container">
+
-
+
-
<div class = "widget span-3">
+
-
<div class = "widget-content">
+
-
<img alt = "Image" src = "http://che.engin.umich.edu/enewsletter/winter2013/images/MSBTlogo2.jpg" style = "margin-bottom: 15px;" />
+
-
+
-
<p>Lorem ipsum dolor sit amet nec, consectetuer adipiscing elit. Aenean commodo ligula eget dolor.</p>
+
-
+
-
<hr>
+
-
+
-
<dl>
+
-
<dt>Telephone:</dt>
+
-
<dd>+1 234 567 8910</dd>
+
-
<dt>Fax:</dt>
+
-
<dd>+1 0198 432 4567</dd>
+
-
<dt>E-mail:</dt>
+
-
<dd><a href="mailto:dtdunham@umich.edu">dtdunham@umich.edu</a></dd>
+
-
</dl>
+
-
</div><!-- .widget-content -->
+
-
</div><!-- .widget -->
+
-
+
-
<div class = "widget span-3">
+
-
<h4 class = "widget-title">Twitter Feed</h4>
+
-
<div class = "widget-content">
+
-
<div class = "twitter-feed" data-user = "envato">
+
-
<div class = "tweet">
+
-
<div class = "text">{text}</div>
+
-
<div class = "time">
+
-
<a href = "{tweet_url}" title = "View this on Twitter" rel = "nofollow">{time}</a>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div><!-- .widget-content -->
+
-
</div><!-- .widget -->
+
-
+
-
<div class = "widget span-3">
+
-
<h4 class = "widget-title">Flicker Stream</h4>
+
-
<div class = "widget-content">
+
-
<div class = "flickr-feed" data-user = "52617155@N08">
+
-
<div class = "image">
+
-
<a class = "overlay" href = "{url}" title = "View this on Flickr" rel = "nofollow"><span class = "overlay-color"></span><img class = "framed-image" src = "{image}" alt = "Image" /></a>
+
-
</div>
+
-
</div>
+
-
</div><!-- .widget-content -->
+
-
</div><!-- .widget -->
+
-
+
-
<div class = "widget span-3">
+
-
<h4 class = "widget-title">Contact</h4>
+
-
<div class = "widget-content">
+
-
<form class = "contact-form" action = "contact.php" method = "post">
+
-
<div class = "form-input">
+
-
<input type = "text" data-prompt = "Name" value = "Name" name = "name">
+
-
</div>
+
-
+
-
<div class = "form-input">
+
-
<input type = "text" data-prompt = "E-mail" value = "E-mail" name = "email">
+
-
</div>
+
-
+
-
<div class = "form-input">
+
-
<textarea data-prompt = "Message" name = "message">Message</textarea>
+
-
</div>
+
-
+
-
<p class = "meta"><span>*</span> All fields are required</p>
+
-
+
-
<button name = "submit" type = "submit">Send Message</button>
+
-
+
-
<div class = "form-failure">
+
-
Message couldn't be sent, please try again.
+
-
</div>
+
-
</form>
+
-
+
-
<div class = "form-success">
+
-
<h5>Thank You</h5>
+
-
<p>
+
-
We received your message and will be in touch with you as soon as possible.
+
-
</p>
+
-
</div>
+
-
</div><!-- .widget-content -->
+
-
</div><!-- .widget -->
+
-
+
-
</div><!--- #page-footer-container -->
+
-
</footer><!--- #page-footer -->
+
-
+
-
+
-
+
-
<footer id = "footer" class = "container-wrap">
+
-
<div id = "footer-container" class = "container">
+
-
+
-
<div class = "row">
+
-
<div id = "footer-left" class = "span-6">
+
-
<p id = "copyright">&copy; 2012 MSBT Template</p>
+
-
+
-
<nav id = "footer-nav-menu">
+
-
<ul>
+
-
<li>
+
-
<a href = "#">Terms</a>
+
-
</li>
+
-
+
-
<li>
+
-
<a href = "#">Privacy Policy</a>
+
-
</li>
+
-
+
-
<li>
+
-
<a href = "#">Contact</a>
+
-
</li>
+
-
</ul>
+
-
</nav>
+
-
</div>
+
-
+
-
<div id = "footer-right" class = "span-6">
+
-
<div id = "social-links">
+
-
<p>Stay Connected</p>
+
-
+
-
<ul>
+
-
<li class = "twitter-link">
+
-
<a href = "#" title = "Twitter">Twitter</a>
+
-
</li>
+
-
<li class = "vimeo-link">
+
-
<a href = "#" title = "Vimeo">Vimeo</a>
+
-
</li>
+
-
<li class = "flickr-link">
+
-
<a href = "#" title = "Flickr">Flickr</a>
+
-
</li>
+
-
<li class = "dribbble-link">
+
-
<a href = "#" title = "Dribbble">Dribbble</a>
+
-
</li>
+
-
<li class = "deviantart-link">
+
-
<a href = "#" title = "DeviantArt">DeviantArt</a>
+
-
</li>
+
-
<li class = "facebook-link">
+
-
<a href = "#" title = "Facebook">Facebook</a>
+
-
</li>
+
-
<li class = "youtube-link">
+
-
<a href = "#" title = "YouTube">YouTube</a>
+
-
</li>
+
-
<li class = "linkedin-link">
+
-
<a href = "#" title = "LinkedIn">LinkedIn</a>
+
-
</li>
+
-
<li class = "googleplus-link">
+
-
<a href = "#" title = "GooglePlus">GooglePlus</a>
+
-
</li>
+
-
</ul>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
+
-
</div><!--- #page-footer-container -->
+
-
</footer><!--- #page-footer -->
+
-
+
</div><!--- #main-wrap -->
</div><!--- #main-wrap -->
</body>
</body>

Latest revision as of 03:27, 18 October 2014

<! -- Michigan Synthetic Biology Team -->

This is an announcement, click the Close button on the right and it will not show again until you restart your browser.

Close
,

MSBT

University of Michigan iGEM Team

Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in E. coli because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing Salmonella, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities.

Abstract

Retrieved from "http://2014.igem.org/Team:Michigan"