Team:Aachen/Notebook/Wetlab2

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__NOTOC__
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#REDIRECT [[Team:Aachen/Notebook/Wetlab]]
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{{CSS/Main}}
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{{Team:Aachen/Stylesheet}}
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{{Team:Aachen/Header}}
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<span id="partners"></span>
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=Our work in the lab=
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<html><ul class="team-grid">
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<!-- Overview -->
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      Summary of April
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/2/25/Aachen_14-10-10_April_iFG.png (file)); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> May </b>
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      <br/><br/>
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      First work on BioBricks
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> June </b>
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      <br/><br/>
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      First creation of own BioBricks
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> July </b>
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      <br/><br/>
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      Improving our chip technology
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> August </b>
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      <br/><br/>
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      Adding Galectin-3 to our repertoire
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> September </b>
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      <br/><br/>
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      Getting into hot phase
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> October </b>
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      <br/><br/>
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      Finalizing our project
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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</ul></html>
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{{Team:Aachen/BlockSeparator}}
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= April =
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== 1st ==
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'''Organization'''
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where to get:
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* key cards and keys for the lab
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* cooled centifuge
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* space in -80&nbsp;°C
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* bottles for 70&nbsp;% ethanol
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* ... (problems of a first year team ^^)
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April Read more about April here...]
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{{Team:Aachen/BlockSeparator}}
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= May =
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== 1st ==
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* gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
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*: &rarr; 120&nbsp;V, 30 min
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*: &rarr; cut out the bands
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May Read more about May here...]
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{{Team:Aachen/BlockSeparator}}
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= June =
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== 3rd ==
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* transformation of 34 BioBricks
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== 4th ==
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* preparation of consumables:
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** fresh 50&nbsp;% glycerol
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** new LB plates with cam and with kanamycin (kan)
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** 60 glas tubes
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** 2&nbsp;L LB<sup>-</sup>
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** 100&nbsp;mL steril glas beads for plating
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* master plates (6 clones per BioBrick) were made
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June Read more about June here...]
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{{Team:Aachen/BlockSeparator}}
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= July =
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== 1st ==
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* transformation efficiency kit is broken
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&rarr; run an agarose gel with the kit plasmids to check for nuclease activity/ bands
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* inventory of the -80°C freezer was done
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== 2nd ==
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* overnight cultures (LB) of K731520 and K1319042 for chips were inoculated
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July Read more about July here...]
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{{Team:Aachen/BlockSeparator}}
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= August =
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== 1st ==
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* made electrocompetent ''E.coli'' rosetta cells.
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* prepared cultures of K1319042 and K131026
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== 2nd ==
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* tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
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* tested K131026 and K1319042 for fluorescence in the plate reader
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* did a heat shock transformation of I746909 into NEB TOP 10 cells
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* did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August Read more about August here...]
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{{Team:Aachen/BlockSeparator}}
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= September =
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== 1st ==
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* 5&nbsp;ml cultures of K1319003 and K1319004
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* plasmid prep
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{| class="wikitable"
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! Plasmid !! DNA [ng/µl]
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|-
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| J23101.K516032 pSB1K3|| 23.5
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|-
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| J23115.K516032 pSB1K3|| 20.5
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|-
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| J04450 pSB1A3|| 57.5
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|-
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| J04450 pSB1K1|| 63.5
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|}
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* over night cultures of K131026 in DH5α and NEB
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September Read more about September here...]
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{{Team:Aachen/BlockSeparator}}
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= October =
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== 1st ==
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* Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the [https://2014.igem.org/Team:Aachen/Notebook/Protocols#Agar_Chips| sensor chip manufacturing] protocol:
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** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250&nbsp;ml Erlenmeyer flasks each containing 50&nbsp;ml LB medium . The flasks were incubated for ~12 hours at 37&nbsp;°C on a shaker.
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October Read more about October here...]
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{{Team:Aachen/Footer}}
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Latest revision as of 14:04, 14 October 2014

  1. REDIRECT Team:Aachen/Notebook/Wetlab
Retrieved from "http://2014.igem.org/Team:Aachen/Notebook/Wetlab2"