Team:Kyoto

From 2014.igem.org

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            <h2>Project Overview</h2>
 
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            <p>Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists’ words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CNN), we thought we could produce cloud by creating <i>E.coli</i> which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in <i>E.coli</i> by introducing genes from <i>Fragilariopsis cylindrus</i> and <i>Ruegeria pomeroyi</i>. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don’t you think it is fantastic if the small organism, <i>E. coli</i>, creates something big, cloud? And this project may have the possibility to solve environmental problems.</p>
 
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            <h3 style="text-indent: 30px;"><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis">Read More... Click Here!</a></h3>
 
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        <h1 id="kyoto-title-magnet"><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation" title="Magnetosome Formation"></a></h1>
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             <h2>Project Overview</h2>
             <h2>Project Overview</h2>
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             <p>We're dreaming of keeping <i>E. coil</i> in hand; moving them here and there. Magneto.coli will realize it. A magnetosome is an organelle seen in magnetotactic bacteria; storing magnetite in it. We believe <i>E. coil</i> with a magnetosome can be moved by magnetism
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             <p>If we are able to move <i>E. coli</i> to the proper places, the achievements of synthetic biology will be more useful. For example, bioremediation, drug delivery, making <i>E. coli</i> pellet without centrifuge, etc. In order to realize this dream, we focused on the magnetosome: a magnetic organelle of magnetotactic bacteria. It consists of a lipid bilayer and a  magnet. <i>E. coli</i> with magnetosome will be moved by magnetism. To realize this amazing <i>E. coli</i>, we started from making the magnetosome. Finally, we detected magnetosome-like vesicles in our transformants!
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. To make magetosomes in <i>E. coil</i>, at least two processes are required; vesicle formation and uptake of iron in it. It had been unclear what genes were necessary for them in <i>E. coil</i>. We focused on vesicle formation through this project, and found <i>mamL/Q</i>, derived from <i>Magnetospirillum magnetotacticum</i>, played an important role in vesicle formation. We did detect organelles in <i>mamL/Q</i>-induced <i>E. coil</i>.</p>
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             <h3><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation">Read More... Click Here!</a></h3>
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             <h3 style="text-indent: 30px;"><a href="https://2014.igem.org/Team:Kyoto/Project/Magnetosome_Formation" style="color: #88ffee;">Read More... Click Here!</a></h3>
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        <h1 id="kyoto-title-dms"><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis" title="DMS Synthesis"></a></h1>
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            <h2>Project Overview</h2>
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            <p>Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists' words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CCN), we thought we could produce cloud by creating <i>E. coli</i> which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in <i>E. coli</i> by introducing genes from <i>Fragilariopsis cylindrus</i> and <i>Ruegeria pomeroyi</i>. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don't you think it is fantastic if the small organism, <i>E. coli</i>, creates something big, cloud? And this project may have the possibility to solve environmental problems.</p>
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            <h3><a href="https://2014.igem.org/Team:Kyoto/Project/DMS_Synthesis" style="color: #ffdd77">Read More... Click Here!</a></h3>
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             <li><a id="kyoto-top-menu-note" href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation" title="Notebook"></a></li>
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Latest revision as of 02:48, 18 October 2014

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Project Overview

If we are able to move E. coli to the proper places, the achievements of synthetic biology will be more useful. For example, bioremediation, drug delivery, making E. coli pellet without centrifuge, etc. In order to realize this dream, we focused on the magnetosome: a magnetic organelle of magnetotactic bacteria. It consists of a lipid bilayer and a magnet. E. coli with magnetosome will be moved by magnetism. To realize this amazing E. coli, we started from making the magnetosome. Finally, we detected magnetosome-like vesicles in our transformants!

Read More... Click Here!

Project Overview

Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists' words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CCN), we thought we could produce cloud by creating E. coli which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in E. coli by introducing genes from Fragilariopsis cylindrus and Ruegeria pomeroyi. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don't you think it is fantastic if the small organism, E. coli, creates something big, cloud? And this project may have the possibility to solve environmental problems.

Read More... Click Here!

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