Team:Aachen/Notebook/Wetlab2

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__NOTOC__
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#REDIRECT [[Team:Aachen/Notebook/Wetlab]]
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{{CSS/Main}}
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{{Team:Aachen/Stylesheet}}
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{{Team:Aachen/Header}}
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<span id="partners"></span>
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=Wetlab=
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A core concept of iGEM is the collaboration in an interdisciplinary student team. Accordingly, students of different age, gender and field of study came together to found our team in Aachen, in order to realize our project with joint forces and enthusiasm for the overall goal. Working closely together, we therefore not only have the chance to learn from each other, but also to excel ourselves. Using our collective creativity and common responsibility, we will create something special in order to make our contribution to synthetic biology.
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<html><ul class="team-grid">
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<!-- Overview -->
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> April </b>
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      <br/><br/>
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      Organization and preparation
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/35/Aachen_team_member_Michael_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> May </b>
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      <br/><br/>
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      First work on BioBricks
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> June </b>
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      <br/><br/>
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      First creation of own BioBricks
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> July </b>
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      <br/><br/>
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      Improving our chip technology
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> August </b>
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      <br/><br/>
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      Adding Galectin-3 to our repertoire
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> September </b>
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      <br/><br/>
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      Getting into hot phase
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> October </b>
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      <br/><br/>
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      Finalizing our project
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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</ul></html>
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{{Team:Aachen/BlockSeparator}}
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= April =
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== 1st ==
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'''Organization'''
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where to get:
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* key cards and keys for the lab
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* cooled centifuge
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* space in -80&nbsp;°C
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* bottles for 70&nbsp;% ethanol
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* ... (problems of a first year team ^^)
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April Read more about April here...]
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{{Team:Aachen/BlockSeparator}}
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= May =
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== 1st ==
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* gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
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*: &rarr; 120&nbsp;V, 30 min
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*: &rarr; cut out the bands
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May Read more about May here...]
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{{Team:Aachen/BlockSeparator}}
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= June =
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== 3rd ==
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* transformation of 34 BioBricks
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== 4th ==
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* preparation of consumables:
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** fresh 50&nbsp;% glycerol
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** new LB plates with cam and with kanamycin (kan)
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** 60 glas tubes
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** 2&nbsp;L LB<sup>-</sup>
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** 100&nbsp;mL steril glas beads for plating
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* master plates (6 clones per BioBrick) were made
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June Read more about June here...]
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{{Team:Aachen/BlockSeparator}}
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= July =
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== 1st ==
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* transformation efficiency kit is broken
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&rarr; run an agarose gel with the kit plasmids to check for nuclease activity/ bands
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* inventory of the -80°C freezer was done
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== 2nd ==
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* overnight cultures (LB) of K731520 and K1319042 for chips were inoculated
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July Read more about July here...]
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{{Team:Aachen/BlockSeparator}}
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= August =
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== 1st ==
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* made electrocompetent ''E.coli'' rosetta cells.
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* prepared cultures of K1319042 and K131026
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== 2nd ==
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* tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
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* tested K131026 and K1319042 for fluorescence in the plate reader
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* did a heat shock transformation of I746909 into NEB TOP 10 cells
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* did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August Read more about August here...]
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{{Team:Aachen/BlockSeparator}}
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= September =
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== 1st ==
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* 5&nbsp;ml cultures of K1319003 and K1319004
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* plasmid prep
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{| class="wikitable"
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|-
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! Plasmid !! DNA [ng/µl]
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|-
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| J23101.K516032 pSB1K3|| 23.5
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|-
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| J23115.K516032 pSB1K3|| 20.5
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|-
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| J04450 pSB1A3|| 57.5
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|-
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| J04450 pSB1K1|| 63.5
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|}
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* over night cultures of K131026 in DH5α and NEB
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September Read more about September here...]
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{{Team:Aachen/BlockSeparator}}
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= October =
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== 1st ==
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* Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the [https://2014.igem.org/Team:Aachen/Notebook/Protocols#Agar_Chips| sensor chip manufacturing] protocol:
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** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250&nbsp;ml Erlenmeyer flasks each containing 50&nbsp;ml LB medium . The flasks were incubated for ~12 hours at 37&nbsp;°C on a shaker.
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[https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October Read more about October here...]
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{{Team:Aachen/Footer}}
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Latest revision as of 14:04, 14 October 2014

  1. REDIRECT Team:Aachen/Notebook/Wetlab
Retrieved from "http://2014.igem.org/Team:Aachen/Notebook/Wetlab2"