Team:Hannover/Protocols/EasyIsolation

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<h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / A simple method for DNA isolation</h1>
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<h1>A simple method for DNA isolation</h1>
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<p class="text">A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.</p>
<p class="text">A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.</p>
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<table style="float:right" style="spadtable"><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/a/ad/Hannover_20141003_Sbd6_klein.jpg
" data-lightbox="galery" data-title="Our DNA-Isolation kit"><img src="https://static.igem.org/mediawiki/2014/thumb/a/ad/Hannover_20141003_Sbd6_klein.jpg/120px-Hannover_20141003_Sbd6_klein.jpg" width="160px" style="center"></a></td></tr><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/c/ce/Hannover_20141003_Sbd3.jpg
" data-lightbox="galery" data-title="Our DNA-Isolation kit"><img src="https://static.igem.org/mediawiki/2014/thumb/a/ad/Hannover_20141003_Sbd6_klein.jpg/120px-Hannover_20141003_Sbd6_klein.jpg" width="160px" style="center"></a></td></tr><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/c/ce/Hannover_20141003_Sbd3.jpg
" data-lightbox="galery" data-title="DNA-Isolation"><img src="https://static.igem.org/mediawiki/2014/thumb/c/ce/Hannover_20141003_Sbd3.jpg/120px-Hannover_20141003_Sbd3.jpg
" data-lightbox="galery" data-title="DNA-Isolation"><img src="https://static.igem.org/mediawiki/2014/thumb/c/ce/Hannover_20141003_Sbd3.jpg/120px-Hannover_20141003_Sbd3.jpg
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<tr><td><h4>Material:</h4></td>
<tr><td><h4>Material:</h4></td>
<tr><td>Bananas</td><td>Tap water</td>
<tr><td>Bananas</td><td>Tap water</td>
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<li>Wait for 10 min</li>
<li>Wait for 10 min</li>
<li>DNA is soaring into the phase of isopropyl (alcohol)</li></ul>
<li>DNA is soaring into the phase of isopropyl (alcohol)</li></ul>
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Latest revision as of 07:01, 15 October 2014

Protocols / A simple method for DNA isolation

A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.

Material:

BananasTap water
Table saltDishwashing liquid as detergent
Washing agent with proteasesHot water (60 °C)
IceTeaspoon
TablespoonFork
Isopropyl (alcohol) (cooled)Coffee filter
Funnel50 mL vessels


Test execution:

  • preparation of extraction solution:
    1. 1 teaspoon table salt
    2. 1 tablespoon dishwashing liquid
    3. 10 drops washing agent
    4. Fill up to 100 mL with tap water
    5. Mixing by shaking
  • Homogenization of a banana with the fork
  • Transfer of 3 teaspoons homogenized banana into a 50mL reaction vessel with 30 mL extraction solution.
  • 15 min incubation at 60 °C
  • 5 min chilling on ice
  • Filter the mixture into a new 50 mL vessel.
  • Overlay the filtered solution with some iosporyl (alcohol)
  • Wait for 10 min
  • DNA is soaring into the phase of isopropyl (alcohol)