Team:WashU StLouis/Notebook/August

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&nbsp;&nbsp;&nbsp; |&nbsp;&nbsp; &nbsp; <a href="https://2014.igem.org/Team:WashU_StLouis/Notebook/September">School Year</a></h3>
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Onwards</a></h3>
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<center> <img style="border: 0px solid ; width: 1024px;" alt="August Calendar" src="https://static.igem.org/mediawiki/2014/9/9c/WashU_August_Calendar.png"> </center> <br><br>
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<td style="vertical-align: top; text-align: center;"><span style="font-weight: bold;">Week Twelve- August 25 - End</span><br><div style="text-align: left;">
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<td style="vertical-align: top; text-align: center;"><span style="font-weight: bold;">Week Twelve- August 25 - 31</span><br><div style="text-align: left;">
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School year started. <br>Continued cloning projects and troubleshooting.<br>Ben starting cloning for modified RBS positive control to test disfunctional reporter protein.<br>Started biobricking.<br>Caroline continued work with the positive control and started biobricking the <i>nif</i> cluster. <br> Richard continued Acetylene Reduction Assays <br>Ben confirmed that Ptet was the issue and swapped out tet promoters to a functional one. Cheryl helped Ben to swap out reporter genes into the light plasmids, and Ben successfully sent in two parts for biobricking. <br> Website started mass compiling information and formatting. Discovered Kompozer to format all the text. Worked on protocol page and notebook pages, waiting for data and graphs to fill pages.
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School year started. <br>Rebstock group continued to use acetylene reduction assay to test nitrogen fixation activity of JM109 and WM1788 under known optimal conditions.<br>  
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Meanwhile Rebstock group started to plan specific protocol and design for BioBricking the <i>nif</i> plasmid.<br>
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Ben starting cloning project to test whether switching the rbs to a comparable one would fix the EYFP expression in the light plasmids. He sent the clones in for sequencing.</i>
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Ben also worked on the title and abstract for the iGEM September 1st deadline.
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Latest revision as of 22:15, 17 October 2014



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