Team:Gaston Day School/Parts

From 2014.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 254: Line 254:
<div class="row">
<div class="row">
-
<H1 id="mainhead" style="color:#072751" class="show" align="center">2014 GDS iGEM Parts Page</p>
+
<h1 id="mainhead" style="color:#072751" class="show" align="center">2014 GDS iGEM Parts Page</p>
 +
            <h2 style="color:#072751">>Parts</h2>
</div>
</div>
                  
                  
Line 301: Line 302:
<div class="col-1-2">
<div class="col-1-2">
<h2 id="head">Cd Detector</h2>
<h2 id="head">Cd Detector</h2>
-
<p class="show" id="pad">
+
<div class="show" id="pad">
-
                         K1320000
+
                         <a href="http://parts.igem.org/Part:BBa_K1320000">
-
                     </p>
+
                        K1320000
 +
                        </a>
 +
<br>
 +
<br>In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.
 +
<br>
 +
<br>2012 Cd Detector Results
 +
<br><img src="https://static.igem.org/mediawiki/2014/2/21/2012_Cd_graph.jpg" alt="6.11.14iso.png" style="width:100%">
 +
<br>
 +
<br>2014 Cd Detector Results
 +
<br><img src="https://static.igem.org/mediawiki/2014/d/db/Cadmium_graph.jpg" alt="6.11.14iso.png" style="width:100%">
 +
 
 +
 
 +
                     </div>
</div>
</div>
<div class="col-1-2">
<div class="col-1-2">
<h2 id="head">GlmY</h2>
<h2 id="head">GlmY</h2>
-
<p class="show" id="pad">
+
<div class="show" id="pad">
-
                         K1320100
+
                         <a href="http://parts.igem.org/Part:BBa_K1320100">
-
                    </p>
+
                        K1320100
-
</div>
+
                        </a>
-
</div>
+
<br>
-
           
+
<br> Gel showing our working GlmY construct. Wells are as follows:
-
<div class="grid-pad">
+
<br>1.Ladder
-
<div class="col-1-2">
+
<br>2.Control
-
<h2 id="head">Project 3</h2>
+
<br>3.GlmY
-
<p class="show" id="pad">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec viverra lorem at metus porta,
+
<br>4.GlmY
-
non mattis quam fermentum. Aliquam elementum ligula arcu, quis pharetra leo dapibus vel. Nullam vulputate erat turpis,
+
<br><img src="https://static.igem.org/mediawiki/2014/thumb/3/39/Working_GlmY_Gel.jpg/336px-Working_GlmY_Gel.jpg" alt="6.11.14iso.png" style="width:100%">
-
in molestie odio elementum sed. Fusce hendrerit suscipit magna, non tempor purus molestie sit amet. Duis eget lacus sit
+
                     </div>
-
amet elit tristique facilisis ac sit amet ligula. Maecenas nec faucibus eros. In malesuada odio felis, feugiat posuere
+
-
ipsum ultricies eget. Donec semper, arcu sit amet rhoncus suscipit, purus mauris varius justo, id dictum turpis lacus
+
-
id nibh. Sed mollis, ipsum id euismod tincidunt, tortor eros fermentum velit, nec cursus mauris nunc sit amet quam.
+
-
Integer pellentesque enim ligula. Morbi pretium ante in iaculis eleifend.Duis et orci id nibh blandit eleifend.
+
-
Vivamus viverra tincidunt sagittis. Donec molestie commodo euismod. Curabitur iaculis condimentum
+
-
pellentesque. Cras quis velit nibh. Duis vitae neque dignissim, viverra lacus a, mollis nibh.</p>
+
-
</div>
+
-
<div class="col-1-2">
+
-
<h2 id="head">Project 4</h2>
+
-
<p class="show" id="pad">
+
-
                        Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec viverra lorem at metus porta,
+
-
non mattis quam fermentum. Aliquam elementum ligula arcu, quis pharetra leo dapibus vel. Nullam vulputate erat turpis,
+
-
in molestie odio elementum sed. Fusce hendrerit suscipit magna, non tempor purus molestie sit amet. Duis eget lacus sit
+
-
amet elit tristique facilisis ac sit amet ligula. Maecenas nec faucibus eros. In malesuada odio felis, feugiat posuere
+
-
ipsum ultricies eget. Donec semper, arcu sit amet rhoncus suscipit, purus mauris varius justo, id dictum turpis lacus
+
-
id nibh. Sed mollis, ipsum id euismod tincidunt, tortor eros fermentum velit, nec cursus mauris nunc sit amet quam.
+
-
Integer pellentesque enim ligula. Morbi pretium ante in iaculis eleifend.Duis et orci id nibh blandit eleifend.
+
-
Vivamus viverra tincidunt sagittis. Donec molestie commodo euismod. Curabitur iaculis condimentum
+
-
pellentesque. Cras quis velit nibh. Duis vitae neque dignissim, viverra lacus a, mollis nibh.
+
-
                     </p>
+
</div>
</div>
</div>
</div>

Latest revision as of 21:23, 17 October 2014

2014 GDS iGEM Parts Page

>Parts

K1320000

In 2012 our team created a cadmium detector part K824008. We also created several other heavy metal detectors, but since our team was unable to gain access to other heavy metals due to our BS1 status, and cadmium was quickly becoming a local issue, we decided to focus on improving our cadmium detector. This first detector was sensitive to 10 mM of cadmium. This year, with the addition of sensitivity tuners, we improved the sensitivity four-fold. We can now detect 2.5 mM of cadmium, which is a much more realistic and useful concentration to find. At 2.5 mM of cadmium, the presence is not necessarily apparent in water, but is still above safe levels and the federal limit.

2012 Cd Detector Results
6.11.14iso.png

2014 Cd Detector Results
6.11.14iso.png
K1320100

Gel showing our working GlmY construct. Wells are as follows:
1.Ladder
2.Control
3.GlmY
4.GlmY
6.11.14iso.png