Team:NCTU Formosa/Notebook/test5

From 2014.igem.org

(Difference between revisions)

Latest revision as of 14:24, 17 October 2014

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8/11

Transformation of NCTU1 (G1)

Transformation of PBAN (G2)

8/12

Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)

Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr

Mini-prep of [P_cons]Ar

Digestion of [P_cons]Ar (ES) (G2)

8/13

Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)

Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)

Electrophoresis of [P_cons](mini)&(dig ES),digestion of [P_cons](XP)

Ligation of [P_cons]+[RBS+BFP+J61048] (G2)

8/14

Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6(G1)

Transformation of the ligation product made yesterday

Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[P_cons],[P_cons+RBS+BFP+J61048] (G2)

8/15

Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6

Transformation of [B0034]Ar (G2)

8/16

Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)

PCR of [B0034]

Electrophoresis of [PSB1K3](mini)&(dig E),[P_cons](dig SP),PCR products of B0034

Mini-prep of [B0034]Ar E.coli

Ligation of [P_cons]+[B0034] +PSB1K3

Digestion of [BFP](ES)

Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[P_cons])

8/17

Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)

None

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 <div class="calendar">
 <ul class="calendar-week">
-<li>Sunday</li>
+<li>Sun.</li>
-<li>Monday</li>
+<li>Mon.</li>
-<li>Tuesday</li>
+<li>Tue.</li>
-<li>Wednesday</li>
+<li>Wed.</li>
-<li>Thursday</li>
+<li>Thu.</li>
-<li>Friday</li>
+<li>Fri.</li>
-<li>Saturday</li>
+<li>Sat.</li>
 </ul>
 <ul class="calendar-day">
 <li>　</li>
 <li>　</li>
-<li>  </li>
+<li>　</li>
+<li>　</li>
+<li>　</li>
 <li>1</li>
 <li>2</li>
-<li>3</li>
-<li>4</li>
 </ul>
 <ul class="calendar-day">
+<li>3</li>
+<li>4</li>
 <li>5</li>
 <li>6</li>
@@ Line 26: / Line 28: @@
 <li>8</li>
 <li>9</li>
-<li>10</li>
-<li>11</li>
 </ul>
 <ul class="calendar-day">
+<li>10</li>
+<li>11</li>
 <li>12</li>
 <li>13</li>
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 <li>15</li>
 <li>16</li>
-<li>17</li>
-<li>18</li>
 </ul>
 <ul class="calendar-day">
+<li>17</li>
+<li>18</li>
 <li>19</li>
 <li>20</li>
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 <li>22</li>
 <li>23</li>
-<li>24</li>
-<li>25</li>
 </ul>
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+<li>24</li>
+<li>25</li>
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 <li>27</li>
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 <li>29</li>
 <li>30</li>
+</ul>
+<ul class="calendar-day">
 <li>31</li>
+<li>　</li>
+<li>　</li>
+<li>　</li>
+<li>　</li>
+<li>　</li>
 <li>　</li>
 </ul>
 </div>
-<div class="calendar-note">
+<div class="calendar-note" style="height:251px;">
-<div  class="single note">none<br></div>
+<div  class="single note">
+<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
+<br></div>
+<div  class="single note">
+<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
+<br></div>
 <div  class="single note">
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p>
+<p>
+<font color="yellow">Transformation of</font> NCTU1 (G1)</p>
+<p>
+<font color="yellow">Transformation of</font> PBAN (G2)</p>
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p>
+<p>
+<font color="yellow">Cultivation of</font> [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p>
+<p>
+<font color="yellow">Cultivation of </font> [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p>
+<p>
+<font color="yellow">Mini-prep of</font> [P<sub>cons</sub>]Ar</p>
+<p>
+<font color="yellow">Digestion of</font> [P<sub>cons</sub>]Ar (ES) (G2)</p>
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p>
+<p>
+<font color="yellow">Mini-prep of</font> [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p>
+<p>
+<font color="yellow">Digestion of</font> [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
+<p>
+<font color="yellow">Electrophoresis of</font> [P<sub>cons</sub>](mini)&(dig ES),digestion of [P<sub>cons</sub>](XP)
+</p><p>
+<font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[RBS+BFP+J61048] (G2)</p>
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p>
+<p>
+<font color="yellow">Electrophoresis of</font> plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p>
+<p>
+<font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6(G1)</p>
+<p>
+<font color="yellow">Transformation of</font> the ligation product made yesterday</p>
+<p>
+<font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN]No.4&5&6,[P<sub>cons</sub>],[P<sub>cons</sub>+RBS+BFP+J61048] (G2)</p>
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p>
+<p>
+<font color="yellow">Digestion of</font> purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p>
+<p>
+<font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6</p>
+<p>
+<font color="yellow">Transformation of</font> [B0034]Ar (G2)</p>
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p>
+<p>
+<font color="yellow">Electrophoresis of</font> the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
+<p>
+<font color="yellow">PCR of</font> [B0034]</p>
+<p>
+<font color="yellow">Electrophoresis of</font> [PSB1K3](mini)&(dig E),[P<sub>cons</sub>](dig SP),PCR products of B0034</p>
+<p>
+<font color="yellow">Mini-prep of</font> [B0034]Ar E.coli</p>
+<p>
+<font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[B0034] +PSB1K3</p>
+<p>
+<font color="yellow">Digestion of</font> [BFP](ES)</p>
+<p>
+<font color="yellow">Electrophoresis of </font> plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[P<sub>cons</sub>])</p>
 <br></div>
 <div  class="single note">
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p>
+<p>
+<font color="yellow">Electrophoresis of</font> the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
 <br></div>
 <div  class="single note">
+<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
 <br></div>
 <div  class="single note">
+<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
 <br></div>
 </div>
 <div class="clear"></div>
 </div>