Team:Brasil-SP/Notebook
From 2014.igem.org
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- | < | + | <!--main content --> |
- | < | + | <table width="70%" align="center"> |
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- | < | + | <h1 align=center>Main Assembly Map</h1> |
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- | </ | + | <p><div align="justify"> The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p> |
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+ | .map_image .map_link:hover {border: dashed 2px #d7ccea;} | ||
+ | .map_image #map_link_1 { width: 120px; height: 82px; top: 15px; left: 1px;} | ||
+ | .map_image #map_link_2 { width: 86px; height: 83px; top: 17px; left: 136px; } | ||
+ | .map_image #map_link_3 { width: 223px; height: 84px; top: 16px; left: 229px; } | ||
+ | .map_image #map_link_4 { width: 138px; height: 86px; top: 14px; left: 464px; } | ||
+ | .map_image #map_link_5 { width: 113px; height: 85px; top: 14px; left: 616px; } | ||
+ | .map_image #map_link_6 { width: 256px; height: 89px; top: 125px; left: 140px; } | ||
+ | .map_image #map_link_7 { width: 351px; height: 87px; top: 258px; left: 221px; } | ||
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+ | .map_image #map_link_9 { width: 111px; height: 80px; top: 153px; left: 978px; } | ||
+ | .map_image #map_link_10 { width: 168px; height: 81px; top: 153px; left: 805px; } | ||
+ | .map_image #map_link_11 { width: 127px; height: 83px; top: 129px; left: 629px; } | ||
+ | </style> | ||
+ | <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/8/87/Mapa.png');"> | ||
+ | <a class="map_link" id="map_link_1" title="" href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a> | ||
+ | <a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a> | ||
+ | <a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> | ||
+ | <a class="map_link" id="map_link_4" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a> | ||
+ | <a class="map_link" id="map_link_5" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a> | ||
+ | <a class="map_link" id="map_link_6" title="" href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> | ||
+ | <a class="map_link" id="map_link_7" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | ||
+ | <a class="map_link" id="map_link_8" title="" href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a> | ||
+ | <a class="map_link" id="map_link_9" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a> | ||
+ | <a class="map_link" id="map_link_10" title="" href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> | ||
+ | <a class="map_link" id="map_link_11" title="" href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> | ||
+ | </div> | ||
- | + | <h1 align=center>Assemblies forms</h1> | |
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<p><strong>Assembly form template</strong></p> | <p><strong>Assembly form template</strong></p> | ||
<p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p> | <p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p> | ||
+ | <div align=center> | ||
<a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a> | <a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a> | ||
+ | </div> | ||
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- | <h1><strong>Life Inside the LAB</ | + | <h1 align=center><strong>Life Inside the LAB</strong></h1> |
- | <thead><h2>July</h2> | + | <thead><h2 align=center>July</h2> |
</thead> | </thead> | ||
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<table> | <table> | ||
<tr> | <tr> | ||
- | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
- | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
- | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
- | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
- | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td>30/06 | <td>30/06 | ||
<ul> | <ul> | ||
- | <li>PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion.</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li> |
</ul> | </ul> | ||
</td> | </td> | ||
<td>01/07 | <td>01/07 | ||
<ul> | <ul> | ||
- | <li>Purification of lasR PCR product </li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li> |
</ul> | </ul> | ||
</td> | </td> | ||
<td>02/07 | <td>02/07 | ||
<ul> | <ul> | ||
- | <li>Transformation of the Biobricks:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
- | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
- | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
<li>Inoculum of the Biobricks sent by iGEM HQ:</li> | <li>Inoculum of the Biobricks sent by iGEM HQ:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316037</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
- | <li>BBa_K316018</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
- | <li>BBa_K316015</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>03/07 | <td>03/07 | ||
<ul> | <ul> | ||
- | <li>Transformation of the Biobricks sent by the Imperial College Team:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li> |
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li> |
- | <li>BBa_K143031</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li> |
</ul> | </ul> | ||
- | <li>Miniprep, Quantification of the plasmidial DNA samples and Restriction Analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
- | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
- | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>07/07 | <td>07/07 | ||
<ul> | <ul> | ||
- | <li>Miniprep, Quantification and Restriction Analysis: | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
- | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
- | <li>BBa_E0840</li> | + | <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
- | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>10/07 | <td>10/07 | ||
<ul> | <ul> | ||
- | <li>Miniprep, Quantification and Restriction Analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_B0015 (restriction analysis fail, repeat)</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li> |
</ul> | </ul> | ||
- | <li>PCR the qteE gene for amplification. We also added the RBS (BBa_K143021) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion.</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li> |
- | <li>Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> |
</ul> | </ul> | ||
</td> | </td> | ||
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<ul> | <ul> | ||
<li>Prepare 40% glycerol solution</li> | <li>Prepare 40% glycerol solution</li> | ||
- | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li>RBS + lasR (BBa_C0079)</li> | + | <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li> |
</ul> | </ul> | ||
- | <li>Purification:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> |
<ul> | <ul> | ||
<li>RBS + qteE</li> | <li>RBS + qteE</li> | ||
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<li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
<li>Prepare X-gal</li> | <li>Prepare X-gal</li> | ||
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</ul> | </ul> | ||
<li>Replate RBS+lasR</li> | <li>Replate RBS+lasR</li> | ||
- | <li>Ligation of PCR qteE in pGEM T-easy vector</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li> |
- | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li> |
</ul> | </ul> | ||
- | <li>Restriction analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>16/07 | <td>16/07 | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Electrophoresis analysis:</li> | <li>Electrophoresis analysis:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
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<td>17/07 | <td>17/07 | ||
<ul> | <ul> | ||
- | <li>Glycerol Stock and Miniprep:<li> | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li> |
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
<li>RBS+lasR</li> | <li>RBS+lasR</li> | ||
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
- | <li>Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
- | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
</ul> | </ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
<ul> | <ul> | ||
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 370: | Line 295: | ||
<td>18/07 | <td>18/07 | ||
<ul> | <ul> | ||
- | <li>Glycerol Stock and Miniprep:</li> | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
- | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
- | <li>Gel Purification:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
- | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
- | <li>Restriction analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 391: | Line 316: | ||
<td>21/07 | <td>21/07 | ||
<ul> | <ul> | ||
- | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 410: | Line 335: | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
- | <li>Miniprep, Restriction analysis and Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
Line 418: | Line 343: | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 427: | Line 352: | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
- | <li>Prepare more competent cells</li> | + | <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li> |
<li>Prepare LB medium (solid and liquid)</li> | <li>Prepare LB medium (solid and liquid)</li> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | <li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | ||
Line 444: | Line 369: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
- | <li>Miniprep:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 455: | Line 380: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 467: | Line 392: | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Restriction analysis (+NdeI enzyme)</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li> |
- | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that | + | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
Line 474: | Line 399: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 484: | Line 409: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 495: | Line 420: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 507: | Line 432: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
- | <li>PCR BBa_K143055 for RBS removal</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li> |
<li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | ||
- | <li>PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector</li> | + | <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 517: | Line 442: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
</tbody> | </tbody> | ||
- | <thead><h2>August</h2> | + | <thead><h2 align=center>August</h2> |
</thead> | </thead> | ||
Line 528: | Line 458: | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
- | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
- | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
- | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
- | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
- | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td></td> | <td></td> | ||
Line 542: | Line 472: | ||
<td>01/08 | <td>01/08 | ||
<ul> | <ul> | ||
- | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
Line 551: | Line 481: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 566: | Line 496: | ||
<li>E. coli DH5-alpha</li> | <li>E. coli DH5-alpha</li> | ||
<li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | ||
- | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
<ul> | <ul> | ||
- | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
<li>Prepare more inoculum</li> | <li>Prepare more inoculum</li> | ||
- | <li>Repeat Restriction analysis</li> | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 582: | Line 512: | ||
<ul> | <ul> | ||
<li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | ||
- | <li>Miniprep:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
- | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
- | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>And once again! Restriction analysis (this BBa_K316016 is tough)</li> | + | <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 600: | Line 530: | ||
<td>07/08 | <td>07/08 | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
- | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 612: | Line 542: | ||
<td>11/08 | <td>11/08 | ||
<ul> | <ul> | ||
- | <li>Prepare Plac BBa_K143055 for sequencing</li> | + | <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li> |
<li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | ||
</ul> | </ul> | ||
Line 618: | Line 548: | ||
<td>12/08 | <td>12/08 | ||
<ul> | <ul> | ||
- | <li>Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
Line 631: | Line 561: | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 637: | Line 567: | ||
<td>14/08 | <td>14/08 | ||
<ul> | <ul> | ||
- | <li>Gel Purification and Ligation in pSB1C3 for BBiobrick standard:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
Line 645: | Line 575: | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
<li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li> | <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li> | ||
</ul> | </ul> | ||
Line 656: | Line 586: | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 665: | Line 595: | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 675: | Line 605: | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
<li>Prepare LB medium (liquid and solid)</li> | <li>Prepare LB medium (liquid and solid)</li> | ||
Line 685: | Line 615: | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 704: | Line 634: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li> |
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 714: | Line 644: | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Repeat restriction analysis adding the enzyme NdeI | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 725: | Line 655: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
- | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
- | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
- | <li>PCR BBa_K143055 with higher melting temperature | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li> |
<ul> | <ul> | ||
</td> | </td> | ||
Line 739: | Line 669: | ||
<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 751: | Line 681: | ||
<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 761: | Line 691: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
- | <thead><h2>September</h2> | + | <thead><h2 align=center>September</h2> |
</thead> | </thead> | ||
Line 770: | Line 703: | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
- | < | + | <th style="background-color:#bfacc0">Monday</th> |
- | < | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | < | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | < | + | <th style="background-color:#bfacc0">Thursday</th> |
- | < | + | <th style="background-color:#bfacc0">Friday</th> |
- | < | + | <th style="background-color:#bfacc0">Saturday</th> |
- | < | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td>01/09 | <td>01/09 | ||
Line 782: | Line 715: | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | ||
<ul> | <ul> | ||
- | <li>Digestion of the part BII with EcoRV | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV. |
</ul> | </ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
Line 790: | Line 723: | ||
<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 802: | Line 735: | ||
<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 827: | Line 760: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 839: | Line 772: | ||
<td>08/09 | <td>08/09 | ||
<ul> | <ul> | ||
- | <li>QuickChange PCR to mutate the cleavage site of the | + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> |
- | <li>Transformation of the QuickCHange PCR product</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 847: | Line 780: | ||
<ul> | <ul> | ||
<li>The QuickChange protocol didn't work well. So try again with more attention!</li> | <li>The QuickChange protocol didn't work well. So try again with more attention!</li> | ||
- | <li>QuickChange PCR to mutate the cleavage site of the | + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> |
- | <li>Transformation of the QuickCHange PCR product</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> |
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
Line 854: | Line 787: | ||
<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 860: | Line 793: | ||
<td>11/09 | <td>11/09 | ||
<ul> | <ul> | ||
- | <li>Prepare inoculum of the mutated BBa_K316037</li> | + | <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
Line 866: | Line 799: | ||
<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 878: | Line 811: | ||
<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li> |
</ul> | </ul> | ||
- | <li>We had far to many difficulties trying to remove the RBS from the BBa_K143055, so we decided to synthesize it using PCR.</li> | + | <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li> |
- | <li>PCR for | + | <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 894: | Line 827: | ||
<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Repeat Ligation</li> | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
<li>Prepare LB medium (solid and liquid)</li> | <li>Prepare LB medium (solid and liquid)</li> | ||
Line 904: | Line 837: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
</ul> | </ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
Line 910: | Line 843: | ||
<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 919: | Line 852: | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> | ||
<ul> | <ul> | ||
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
Line 927: | Line 860: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
- | <li>Gel Purification of the BBa_K143015</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
</ul> | </ul> | ||
</td> | </td> | ||
Line 938: | Line 871: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 945: | Line 878: | ||
<td>17/09 | <td>17/09 | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
Line 955: | Line 888: | ||
<td>18/09 | <td>18/09 | ||
<ul> | <ul> | ||
- | <li>Replate BBa_K143015. We forgot the X-gal :(</li> | + | <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
Line 967: | Line 900: | ||
<td>22/09 | <td>22/09 | ||
<ul> | <ul> | ||
- | <li>Inoculum of the BBa_K143015</li> | + | <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
- | <li>Assembly KXIV:</li> | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 977: | Line 910: | ||
<td>23/09 | <td>23/09 | ||
<ul> | <ul> | ||
- | <li>Assembly KXIV:</li> | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
- | <li>Miniprep and | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
</ul> | </ul> | ||
</td> | </td> | ||
<td>24/09 | <td>24/09 | ||
<ul> | <ul> | ||
- | <li>Assembly KXIV:</li> | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 996: | Line 929: | ||
<td>25/09 | <td>25/09 | ||
<ul> | <ul> | ||
- | <li>Assembly KXIV</li> | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
<ul> | <ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
Line 1,004: | Line 937: | ||
<td>26/09 | <td>26/09 | ||
<ul> | <ul> | ||
- | <li>Assembly KXIV:</li> | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
- | <li>Assemnlies KII, KV and | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 1,021: | Line 956: | ||
<td>29/09 | <td>29/09 | ||
<ul> | <ul> | ||
- | <li> | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
- | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
- | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 1,031: | Line 968: | ||
<td>30/09 | <td>30/09 | ||
<ul> | <ul> | ||
- | <li> | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
<ul> | <ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
Line 1,044: | Line 983: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
- | <thead><h2>October</h2> | + | <thead><h2 align=center>October</h2> |
</thead> | </thead> | ||
Line 1,053: | Line 997: | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
- | < | + | <th style="background-color:#bfacc0">Monday</th> |
- | < | + | <th style="background-color:#bfacc0">Tuesday</th> |
- | < | + | <th style="background-color:#bfacc0">Wednesday</th> |
- | < | + | <th style="background-color:#bfacc0">Thursday</th> |
- | < | + | <th style="background-color:#bfacc0">Friday</th> |
- | < | + | <th style="background-color:#bfacc0">Saturday</th> |
- | < | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
<td></td> | <td></td> | ||
Line 1,065: | Line 1,009: | ||
<td>01/10 | <td>01/10 | ||
<ul> | <ul> | ||
- | <li> | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
- | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
Line 1,080: | Line 1,026: | ||
<td>06/10 | <td>06/10 | ||
<ul> | <ul> | ||
- | <li> | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
<ul> | <ul> | ||
- | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
- | <li>Assemblies KIX, | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, |
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
+ | </ul> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td>07/10 | ||
+ | <ul> | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
+ | </ul> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td>08/10 | ||
+ | <ul> | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
+ | </ul> | ||
+ | <ul> | ||
+ | </td> | ||
+ | <td>09/10 | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
+ | <ul> | ||
+ | <li>Inoculum</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td>10/10 | ||
+ | <ul> | ||
+ | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
+ | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
</td> | </td> | ||
- | |||
- | |||
- | |||
- | |||
<td>11/10</td> | <td>11/10</td> | ||
<td>12/10</td> | <td>12/10</td> | ||
Line 1,132: | Line 1,117: | ||
</html> | </html> | ||
+ | {{:Team:Brasil-SP/Templates/Footer}} |
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