Team:BNU-China/home.html

From 2014.igem.org

(Difference between revisions)

Latest revision as of 03:40, 18 October 2014

1 2

We are iGEMers from BNU-China, we are going to tell you a story about E.coli Prometheus ...

Mr E.coli Prometheus is a philanthropic bacterium. One day, he saw many poor peanuts living in so tough a condition that they couldn't grow up nor to flower. He was determined to do something and help!

He turned to us. He asked us for the power to collect molybdate and send them to poor peanuts. We told him: " Since you were not naturally born with the power, for the sake of other lives, you must die after." But he was as determined as ever.

With his power, E.coli Prometheus collected molybdate ...

He sent the precious micro-nutrient to those peanuts everyday. The peanuts became more and more healthier and was about to flower ...

Sadly, E.coli Prometheuspassed away before he saw the flowers of peanut. He suicided as promised. His body changed into nutrition for peanut after his death.
He is honorable.

THE STORY OF E.COLI PROMETHEUS

Our work

The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots.

We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.

Wiki Design

Art design, pictures, figures and the latest experiment results were put on the wiki, an important platform to show our works.

ModA & INPN

ModA we use in our project can bind the molybdate, and INPN can display ModA on E.coli surface.

Chemotaxis

We use McfR plasmid to give E.coli the capacity of Chemotaxis, so that those E.coli carrying molybdate can "swim" to the root of peanut.

Modeling

We have made a model of peanut roots and E.coli in surrounding environment to simulate our idea in soil.

Latest Works

Prev Next

The Story of E.coli Prometheus

BNU-China

@@ Line 20: / Line 20: @@
          <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/e/e4/Bnu_s2.jpg" alt="2" /> </div>
          <div class="showcase-caption">
-				Mr <i>E.coli Prometheus</i> is a philanthropic man. One day, he saw plenty poor peanuts living in a condition that was rough for them to grow up nor to flower. He made up his mind that he must to do something to help! </div>
+				Mr <i>E.coli Prometheus</i> is a philanthropic bacterium. One day, he saw many poor peanuts living in so tough a condition that they couldn't grow up nor to flower. He was determined to do something and help! </div>
        </div>
        		 <!--2-->
@@ Line 26: / Line 26: @@
          <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/6/60/Bnu_s3.jpg" alt="3" /> </div>
          <div class="showcase-caption">
-				Then he found us for help, he asked for the power to collect molybdate and send them to peanut. We told him: " Since the power is not belong to you in nature, for the sake of the other life, you will die after this." But he didn't change his mind for one second.</div>
+				He turned to us. He asked us for the power to collect molybdate and send them to poor peanuts. We told him: " Since you were not naturally born with the power, for the sake of other lives, you must die after." But he was as determined as ever.</div>
        </div>
        		<!--3-->
@@ Line 33: / Line 33: @@
          <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/1/11/Bnu_s4.jpg" alt="4" /> </div>
          <div class="showcase-caption">
-				<i>E.coli Prometheus</i> collected many molybdate with the power ...</div>
+				With his power, <i>E.coli Prometheus</i> collected molybdate ...</div>
        </div>
        		<!--4-->
@@ Line 40: / Line 40: @@
          <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/9/9c/Bnu_s5.jpg" alt="5" /> </div>
           <div class="showcase-caption">
-				He sent the precious micronutrient to those peanut everyday. The peanuts became more and more healthier and was about to flower ...
+				He sent the precious micro-nutrient to those peanuts everyday. The peanuts became more and more healthier and was about to flower ...
 		</div>
        </div>
@@ Line 48: / Line 48: @@
          <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/3/3a/Bnu_s6.jpg" alt="4" /> </div>
          <div class="showcase-caption">
-				Sadly, <i>E.coli Prometheus</i> flied to heaven before he saw the flowers of peanut. He died as we informed him before. His body changed into nutrition for peanut after his death. </br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;He is honorable.</div>
+				Sadly, <i>E.coli Prometheus</i>passed away before he saw the flowers of peanut. He suicided as promised. His body changed into nutrition for peanut after his death. </br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;He is honorable.</div>
        </div>
        <!--end of silde content-->
@@ Line 59: / Line 59: @@
      <!-- Begin Top Columns -->
-     <h2>What We Do</h2>
+     <h2>Our work</h2>
      <div class="two-third">
-       <p>Nitrogen fixing ability of rhizobium is significant for the growth of grass family. The stronger of its ability, the less chemical fertilizer will be used. Molybdenum is a key integral part of nitrogen fixing enzyme in rhizobium and Mo is also important for the growth of plants themselves. However, Mo is heavy mental. The abuse of it will cause environment pollution. Hence, spot application of Mo is of great importance. A plasmid was transfer into E.coli Promethus to make it express fusion protein, one side of which anchors on the out membrane and the other side of which,ModA,catches molybdate. With the help of organic acid tendency system, our Promethus will head for the roots. A suicide system depending on time is also design for the reason of bio-safety. E.coli Promethus will deliver Mo directly to the root of plants. This will also open a new chapter of biological fertilizer.
+       <p>The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots. <p/>
+<p>We transferred our designed plasmids into <i>E.coli</i> "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "<i>Prometheus</i>" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns.   <i>E.coli Prometheus</i> will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.
      </div>    <!-- End Top Columns -->
@@ Line 72: / Line 74: @@
      <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/b/b6/Bnu_icon1.png" alt="" class="center" width="22%"  /><br />
        <h4 class="center">Wiki Design</h4>
-       <p class="center">Art design, pictures, figures and the latest experiment results were put on the wiki in time. Wiki is an important platform to show our works.</p>
+       <p class="center">Art design, pictures, figures and the latest experiment results were put on the wiki, an important platform to show our works.</p>
      </div>
-     <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/6/62/Bnu_icon2.png" alt="" class="center" width="22%" /><br />
+     <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/6/62/Bnu_icon2.png" alt="" class="center" style="width:42% !important" /><br />
        <h4 class="center">ModA & INPN</h4>
-       <p class="center">ModA we used in our project is a kind of protein that can bind the molybdate and INPN can display ModA on E.coli's surface.</p>
+       <p class="center">ModA we use in our project can bind the molybdate, and INPN can display ModA on <i>E.coli</i> surface.</p>
      </div>
      <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/b/bb/Bnu_icon3.png" alt="" class="center" width="22%"  /><br />
        <h4 class="center">Chemotaxis</h4>
-       <p class="center">We Used McfR plasmid to give the E.coli the capacity of Chemotaxis, so that those E.coli with molybdate can swim to the root of peanut.</p>
+       <p class="center">We use McfR plasmid to give <i>E.coli</i> the capacity of Chemotaxis, so that those <i>E.coli</i> carrying molybdate can "swim" to the root of peanut.</p>
      </div>
      <div class="one-fourth last"> <img src="https://static.igem.org/mediawiki/2014/f/fc/Bnu_icon4.png" alt="" class="center" width="22%" /><br />
        <h4 class="center">Modeling</h4>
-       <p class="center">We have made the model of peanut root and E.coli in environment to show how our thought be realized out of lab.</p>
+       <p class="center">We have made a model of peanut roots and <i>E.coli</i> in surrounding environment to simulate our idea in soil.</p>
      </div>
      <div class="clear"></div>
@@ Line 103: / Line 105: @@
 <li> <a href="https://static.igem.org/mediawiki/2014/6/67/Bnu_lw4.jpg" rel="prettyPhoto" title="We consulted farmers about planting peanut in Gaomi."> <span class="overlay zoom">Human Practice</span><img src="https://static.igem.org/mediawiki/2014/b/b4/Bnu_c4.jpg" alt="" /> </a> </li>
-<li> <a href="https://static.igem.org/mediawiki/2014/b/bf/Bnu_0135.jpg" rel="prettyPhoto" title="This picture shows the chemotaxis of different E.coli in different acids."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/6/68/Bnu_c5.jpg" alt="" /> </a> </li>
+<li> <a href="https://static.igem.org/mediawiki/2014/b/bf/Bnu_0135.jpg" rel="prettyPhoto" title="This picture shows the chemotaxis of different <i>E.coli</i> in different acids."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/6/68/Bnu_c5.jpg" alt="" /> </a> </li>
 <li> <a href="https://static.igem.org/mediawiki/2014/f/f8/Bnu_cb6.jpg" rel="prettyPhoto" title="Capillary assay:The capillary tubes were incubated in the bacterial solution."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/f/f3/Bnu_c6.jpg" alt="" /></a></li>