Team:Gothenburg

From 2014.igem.org

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<b>Woopwoop!</b>
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<h2>Introduction</h2>
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<p>Our project intends to create a generation counter in Saccharomyces cerevisiae. The yeast cell would express fluorescent proteins of different colors according to the number of daughter cells spawn. Nowadays, the replicative age of a yeast cell is determined by counting the budding scars with the help of a microscope. This process is time-consuming and not automated. Once our idea is implemented, it would be possible to use a flow cytometric device to sort the cells according to their color and, consequently, generation number. To achieve that, we are building genetic circuits with a logical AND gate ensuring generation specific expression of different fluorescent proteins.
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<img src="https://static.igem.org/mediawiki/2014/3/30/TeamGBG_Explanation_Cute.jpg"
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Gothenburg&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:Gothenburg"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Gothenburg/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Gothenburg"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Gothenburg/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Gothenburg/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Gothenburg/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Gothenburg/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Gothenburg/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
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                <li id="t-upload"><a href="/Special:Upload"
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                  title="Upload files [u]" accesskey="u">Upload file</a>
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                <li id="t-specialpages"><a href="/Special:SpecialPages"
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                  title="List of all special pages [q]" accesskey="q">Special pages</a>
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                <li><a href='/Special:Preferences'>My preferences</a></li>
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        <li id="privacy"><a href="/2014.igem.org:Privacy_policy" title="2014.igem.org:Privacy policy">Privacy policy</a></li>
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        <li id="disclaimer"><a href="/2014.igem.org:General_disclaimer" title="2014.igem.org:General disclaimer">Disclaimers</a></li>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<!-- Links to other team pages -->
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<ul>
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<li><a href="https://2014.igem.org/Team:Gothenburg">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Gothenburg">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:Gothenburg/Attributions">Attributions</a> </li>
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<td > </td>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>Do not use flash in wiki code. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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Latest revision as of 12:15, 17 October 2014

TemplateUp

Introduction

Our project intends to create a generation counter in Saccharomyces cerevisiae. The yeast cell would express fluorescent proteins of different colors according to the number of daughter cells spawn. Nowadays, the replicative age of a yeast cell is determined by counting the budding scars with the help of a microscope. This process is time-consuming and not automated. Once our idea is implemented, it would be possible to use a flow cytometric device to sort the cells according to their color and, consequently, generation number. To achieve that, we are building genetic circuits with a logical AND gate ensuring generation specific expression of different fluorescent proteins.