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Team:Evry/Interlab Study/09-28-2014
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<i>PSB1C3+E0240+J23109</i>: <br> | <i>PSB1C3+E0240+J23109</i>: <br> | ||
| - | Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C. | + | PCR colony of 8 colonies for each construction following protocol table 1 and 2. |
| + | <img src="https://static.igem.org/mediawiki/2014/a/a1/109gel.jpg" alt="gel" /> <br> | ||
| + | Pre-culture from colony 1 for glycerol stock and TECAN in LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
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<br> | <br> | ||
<i>PSB1C3+E0240+J23114</i>: <br> | <i>PSB1C3+E0240+J23114</i>: <br> | ||
| - | Measure of the cells' growth and fluorecence on TECAN infiniteM200 ( | + | Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 20-60nM (15h00-22h00).<br> |
<img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="IMAGE DU PLAN DE PLAQUE" /> | <img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="IMAGE DU PLAN DE PLAQUE" /> | ||
<br> | <br> | ||
Latest revision as of 08:19, 16 October 2014
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23109:
PCR colony of 8 colonies for each construction following protocol table 1 and 2.
Pre-culture from colony 1 for glycerol stock and TECAN in LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23114:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 20-60nM (15h00-22h00).
Sep 28
