Team:Cambridge-JIC/Guide/Transformation
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- | + | =Transformation= | |
- | + | This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days. | |
- | + | ||
- | + | See the [[Team:Cambridge-JIC/Guide/Transformation/Outline| outline]] for an overview of the process. | |
- | + | ||
- | + | ==Links== | |
- | + | The steps in transformation are roughly: | |
- | </html> | + | |
+ | 1. [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro | Electroporate Agrobacterium]] | ||
+ | |||
+ | 2. [[Team:Cambridge-JIC/Guide/Care/PreparingSpores | Prepare spores]] | ||
+ | |||
+ | 3. [[Team:Cambridge-JIC/Guide/Transformation/Cocultivation | Cocultivate]] or use [[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| AgarTrap]] | ||
+ | |||
+ | 4. [[Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants| Select transformants]] | ||
+ | <html><br><br><br><br><br><br></html> |
Latest revision as of 15:38, 17 October 2014
Transformation
This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.
See the outline for an overview of the process.
Links
The steps in transformation are roughly:
1. Electroporate Agrobacterium
3. Cocultivate or use AgarTrap