WikitemplateB project

From 2014.igem.org

(Difference between revisions)
 
(26 intermediate revisions not shown)
Line 3: Line 3:
<style>
<style>
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
-
body {background-color:black}
 
</style>
</style>
<!--  here ends the section that changes the default wiki template to a white full width background -->
<!--  here ends the section that changes the default wiki template to a white full width background -->
 +
<!-- beginning of your page -->
<!-- beginning of your page -->
Line 13: Line 13:
<table id="menu" width="100%"  cellspacing="0" height="135px">
<table id="menu" width="100%"  cellspacing="0" height="135px">
<tr>
<tr>
-
<td  bgColor="#000000"></td>
+
<td  bgColor="#FFFFFF"></td>
-
<td valign="bottom"  width="975px" align="center" bgColor="#000000">
+
<td valign="bottom"  width="975px" align="center" bgColor="#FFFFFF">
<table align="center">
<table align="center">
<tr height="50px">
<tr height="50px">
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
-
<a href="https://2014.igem.org/WikitemplateB_home"style="text-decoration:none;color:#FFFFFF">[HOME] </a> </td>  
+
<a href="https://2014.igem.org/WikitemplateB_home"style="text-decoration:none;color:#1C140D">HOME </a> </td>  
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
-
<a href="https://2014.igem.org/WikitemplateB_project" style="text-decoration:none;color:#FFFFFF">[PROJECT]</a> </td>
+
<a href="https://2014.igem.org/WikitemplateB_project" style="text-decoration:none;color:#1C140D">PROJECT</a> </td>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
-
<a href="" style="text-decoration:none;color:#FFFFFF">[PARTS]</a></td>  
+
<a href="https://2014.igem.org/WikitemplateB_parts" style="text-decoration:none;color:#1C140D">PARTS</a></td>  
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>  
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>  
-
<a href="" style="text-decoration:none;color:#FFFFFF">[TEAM] </a> </td>
+
<a href="https://2014.igem.org/WikitemplateB_team" style="text-decoration:none;color:#1C140D">TEAM </a> </td>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>   
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>   
-
<a href="" style="text-decoration:none;color:#FFFFFF">[NOTEBOOK] </a> </td>
+
<a href="https://2014.igem.org/WikitemplateB_notebook" style="text-decoration:none;color:#1C140D">NOTEBOOK </a> </td>
-
<td width="162px" align="center" onMouseOver="this.bgColor='#364135'" onMouseOut="this.bgColor='#000000'" bgColor=#000000>   
+
<td width="162px" align="center" onMouseOver="this.bgColor='#FEE5AD'" onMouseOut="this.bgColor='#A1DBB2'" bgColor=#A1DBB2>   
-
<a href="" style="text-decoration:none;color:#FFFFFF">[SAFETY] </a></td>
+
<a href="https://2014.igem.org/WikitemplateB_safety" style="text-decoration:none;color:#1C140D">SAFETY </a></td>
</tr>
</tr>
-
<tr> <td colspan="5"></td></tr>
+
<tr> <td colspan="5"></td>  
 +
<td align="center" width="162px"><a href="https://igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a></td>
 +
</tr>
</table>
</table>
</td>
</td>
-
<td  bgColor="#000000"></td>
+
 
 +
 
 +
<td  bgColor="#FFFFFF"></td>
</tr>
</tr>
</table>
</table>
 +
 +
<!-- end of menu -->
<!-- end of menu -->
 +
<!-- navigation for project page-->
 +
<table width="100%" align="center">
 +
<style bgColor= "#000000"></style>
 +
<tr>
 +
<td  align = center width="700px"  height="300px" bgColor="#FFFFFF"></td>
-
<!-- Page content begins -->
+
<td width="700px"  height="300px" bgColor="#FFFFFF" >
 +
<h3>
 +
</h3>
 +
One E.coli Has One Function
 +
What is our project at 2014?
 +
Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli.
 +
Concretely, we use two kinds of E.coli. One catches Cadmium.　the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli).
 +
To use these E.coli. Finally cadomiun is catched(wastewater is be clean).
 +
What experiment we did?
-
<!-- navigation for project page-->
+
・How to check E.coli's chemotaxis?
-
<table width="70%" align="center" >
+
We make use of other igem team’s　assay.
-
<style bgColor= "#000000"></style>
+
Using soft agar plate.we let E.coli siwm.
-
<tr>
+
-
<td  align = center width="500px"  height="400px" bgColor="#000000"><img align = middle src="https://copy.com/tk3IkTcnC0fy2cmC" width="500px"></td>
+
-
<td width="500px"  height="300px"  bgColor="#000000" >
+
・We made a plasmid
-
<h3><font color="white">REColi: Signal amplification and formattable bacterial memory by DNA edition.</font>
+
Our project need to two kind of plasmid.
-
</h3>
+
we made one plasmid.And we have to make one more plasmid.
-
<font color="white">
 
-
We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.
 
-
</font>
 
</html>
</html>

Latest revision as of 07:02, 8 October 2014

One E.coli Has One Function What is our project at 2014? Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli. Concretely, we use two kinds of E.coli. One catches Cadmium.　the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli). To use these E.coli. Finally cadomiun is catched(wastewater is be clean). What experiment we did? ・How to check E.coli's chemotaxis? We make use of other igem team’s　assay. Using soft agar plate.we let E.coli siwm. ・We made a plasmid Our project need to two kind of plasmid. we made one plasmid.And we have to make one more plasmid.

Retrieved from "http://2014.igem.org/WikitemplateB_project"