Team:Pitt/BuildingFoundations

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<h2>Building the Foundations</h2>
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<h2>Experiments</h2>
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<p>Page under construction.</p>
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<p>The experimental section of our project consisted of three main parts:</p>
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<ol start="1" type="1"><li>Optimizing a transformation protocol for a new strain of bacteria - Propionibacterium acnes.</li>
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<li>Isolate a strong, constitutive promoter from Mycobacteria smegmentis for use in future P. acnes parts.</li>
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<li>Reapply several BioBrick  parts to a new plasmid backbone for use in P. acnes as a skin probiotic.</li></ol>
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<a class = "next_link" "href = "https://2014.igem.org/Team:Pitt/Protocol_Design">Protocol Design</a> <a class = "next_link" href = "https://2014.igem.org/Team:Pitt/HSP60_Promoter">HSP60 Promotor</a>
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<img src = "https://static.igem.org/mediawiki/2014/8/84/Experimental_design.png">
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Protocol_Design/Intro">Intro</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Protocol_Design/Methods">Methods and Timeline</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Protocol_Design/Results">Results and Conclusions</a></li>
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<img src = "https://static.igem.org/mediawiki/2014/e/e2/HSP60_Promoter.png">
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/HSP60_Promoter/Intro">Intro</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/HSP60_Promoter/Methods">Methods and Timeline</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/HSP60_Promoter/Results">Results and Conclusions</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Cathelicidin/Intro">Intro</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Cathelicidin/Methods">Methods and Timeline</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Melanin/Intro">Intro</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Melanin/Methods">Methods and Timeline</a></li>
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<li><a class = "next_link" href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Melanin/Results">Results and Conclusions</a></li>
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Latest revision as of 12:03, 17 October 2014

Experiments

The experimental section of our project consisted of three main parts:

  1. Optimizing a transformation protocol for a new strain of bacteria - Propionibacterium acnes.
  2. Isolate a strong, constitutive promoter from Mycobacteria smegmentis for use in future P. acnes parts.
  3. Reapply several BioBrick parts to a new plasmid backbone for use in P. acnes as a skin probiotic.








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