Team:Tokyo Tech/Parts

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<title>Tokyo_Tech</title>
<title>Tokyo_Tech</title>
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<img src="https://static.igem.org/mediawiki/2014/thumb/b/b7/Tokyo_Tech_Logo.png/613px-Tokyo_Tech_Logo.png" height="150" /></h1></div>
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        <div align="right"><img src="https://static.igem.org/mediawiki/2014/b/b4/Tokyo_Tech_Logo2.png" height="150" /></div>
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<a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/thumb/8/84/Tokyo_Tech_iGEM_logo.png/794px-Tokyo_Tech_iGEM_logo.png" width="90" /></a></div>
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<ul>
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<li class="current_page_item"><a href="https://2014.igem.org/Team:Tokyo_Tech">Home</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech">Home</a></li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Project">Project</a></li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Project">Project</a></li>
<li><a href="#">Experiment</a>
<li><a href="#">Experiment</a>
  <ul>
  <ul>
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        <li><a href="#" style="width:400px; margin-left:-135px;">C4HSL-dependent 3oxoC12HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux and Prhl reporter assay" style="width:400px; margin-left:-135px;">Plux and Prhl reporter assay</a></li>
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        <li><a href="#" style="width:400px; margin-left:-135px;">3oxoC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Improved Prhl reporter assay</a></li>
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        <li><a href="#" style="width:400px; margin-left:-135px;">Symbiosis confirmation by co-culture </a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
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        <li><a href="#" style="width:400px; margin-left:-135px;">Prhl reporter rassay </a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
  </ul>
  </ul>
</li>
</li>
<li><a href="#">Modeling</a>
<li><a href="#">Modeling</a>
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                          <ul>
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                                <li><a href="#" style="width:260px; margin-left:-63px;">なぞなぞ</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Overview" style="width:400px; margin-left:-135px;">Overview</a></li>
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                                <li><a href="#" style="width:260px; margin-left:-63px;">Parameter sensitivity analysis</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Growth Conditions For Company And Customer" style="width:400px; margin-left:-135px;">Growth Conditions For Company And Customer</a></li>
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                                <li><a href="#" style="width:260px; margin-left:-63px;">The effect of economic wave</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Analysis of C4HSL-dependent Switch" style="width:400px; margin-left:-135px;">Analysis of C4HSL-dependent Switch</a></li>
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                            </ul>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Economic Wave"  style="width:400px; margin-left:-135px;">Economic Wave</a></li>
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                          </ul>
                         </li>
                         </li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
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<li class="current_page_item"><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:45px; padding-top:4px;">Policy and Practices</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li>
<li><a href="#">More</a>
<li><a href="#">More</a>
    <ul>
    <ul>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Attribution_and_Contribution" style="width:240px; margin-left:-53px;">Attribution and Contribution</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Attribution_and_Contribution" style="width:240px; margin-left:-53px;">Attribution and Contribution</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Collaboration" style="width:240px; margin-left:-53px;">Collaboration</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Collaboration" style="width:240px; margin-left:-53px;">Collaboration</a></li>
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        <li><a href="#" style="width:240px; margin-left:-53px;">Judging Form</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Judging_Form" style="width:240px; margin-left:-53px;">Judging Form</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Sponsors" style="width:240px; margin-left:-53px;">Sponsors</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Sponsors" style="width:240px; margin-left:-53px;">Sponsors</a></li>
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        <li><a href="https://igem.org/Team.cgi" style="width:240px; margin-left:-53px;">Official Team Page</a></li>
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        <li><a href="https://igem.org/Team.cgi?id=1529" style="width:240px; margin-left:-53px;">Official Team Page</a></li>
    </ul>
    </ul>
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</li>
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      <h2 class="title"><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practice">Parts</a></h2>
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      <h2 class="title">Parts</h2>
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             <span class="meta">Each parts give us a whole new experience </span>
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             <span class="meta">Each part gives us a whole new experience </span>
             <div class="entry-long">
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              <div id="gototop"><a href="#"><img src="https://static.igem.org/mediawiki/2014/5/55/Tokyo_Tech_Go-to-top-icon.png" height="50" /></a></div>
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               <h3>&nbsp;</h3>
               <h3>&nbsp;</h3>
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               <span class="head">Favorite Tokyo Tech 2014 iGEM Team Parts</span><br />
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               <h2>Favorite Tokyo Tech 2014 iGEM Team Parts
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              <br />
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                <br />
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               <br />
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               </h2>
               <div align="center"><table width="799" border="1">
               <div align="center"><table width="799" border="1">
                 <tr bgcolor="#DFDFDF">
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                 <tr class="underName">
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                   <td>&nbsp;</td>
                   <td>&nbsp;</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K1529301">K1529301</a></td>
+
                   <td><a href="http://parts.igem.org/Part:BBa_K1529301">BBa_K1529301</a></td>
                   <td>Composite</td>
                   <td>Composite</td>
                   <td>Prhl(RL)-GFP</td>
                   <td>Prhl(RL)-GFP</td>
                   <td>Kohdai Hibi</td>
                   <td>Kohdai Hibi</td>
-
                   <td>801 </td>
+
                   <td>990 </td>
                 </tr>
                 </tr>
                 <tr class="underName">
                 <tr class="underName">
                   <td>&nbsp;</td>
                   <td>&nbsp;</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K1529265">K1529265</a></td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a></td>
                   <td>Composite</td>
                   <td>Composite</td>
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                   <td>Plux-CmR-RhlI</td>
+
                   <td>Prhl(RL)-CmR-LasI</td>
                   <td>Kohdai Hibi</td>
                   <td>Kohdai Hibi</td>
                   <td>1435</td>
                   <td>1435</td>
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                 <tr class="underName">
                 <tr class="underName">
                   <td>&nbsp;</td>
                   <td>&nbsp;</td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K1529263">K1529263</a></td>
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                   <td><a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a></td>
                   <td>Composite</td>
                   <td>Composite</td>
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                   <td>Plux-CmR-LasI</td>
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                   <td>Plux-CmR-RhlI</td>
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                   <td>Kohdai Hibi</td>
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                   <td>Shoko Suzuki </td>
                   <td>1435</td>
                   <td>1435</td>
                 </tr>
                 </tr>
               </table>
               </table>
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                 <br />
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                 <p>&nbsp;</p>
               </div>
               </div>
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               <p><span class="head">Tokyo Tech 2013 iGEM Team Parts</span></p>
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               <h2>Tokyo Tech 2014 iGEM Team Parts</h2>
               <div align="center">
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                 <table width="799" border="1">
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                   <tr class="underName">
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                     <td>&nbsp;</td>
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                     <td><a href="http://parts.igem.org/Part:BBa_K1529311">K1529311</a></td>
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                     <td><a href="http://parts.igem.org/Part:BBa_K1529311">BBa_K1529311</a></td>
                     <td>Composite</td>
                     <td>Composite</td>
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                     <td>Prhl(RL)-GFP</td>
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                     <td>Prhl(LR)-GFP</td>
                     <td>Kohdai Hibi</td>
                     <td>Kohdai Hibi</td>
-
                     <td>801 </td>
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                     <td>990 </td>
                   </tr>
                   </tr>
                   <tr class="underName">
                   <tr class="underName">
                     <td>&nbsp;</td>
                     <td>&nbsp;</td>
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                     <td><a href="http://parts.igem.org/Part:BBa_K1529321">K1529321</a></td>
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                     <td><a href="http://parts.igem.org/Part:BBa_K1529321">BBa_K1529321</a></td>
                     <td>Composite</td>
                     <td>Composite</td>
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                     <td>Prhl(a)-GFP</td>
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                     <td>Prhl(RR)-GFP</td>
                     <td>Kohdai Hibi</td>
                     <td>Kohdai Hibi</td>
-
                     <td>801</td>
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                     <td>990</td>
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                  </tr>
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                  <tr class="underName">
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                    <td>&nbsp;</td>
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                    <td><a href="http://parts.igem.org/Part:BBa_K1529264">K1529264</a></td>
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                    <td>Composite</td>
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-
                    <td>Plux-CmR-LuxI</td>
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                    <td>Kohdai Hibi</td>
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-
                    <td>1411</td>
+
-
                  </tr>
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                  <tr class="underName">
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-
                    <td>&nbsp;</td>
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                    <td><a href="http://parts.igem.org/Part:BBa_K1529302">K1529302</a></td>
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                    <td>Composite</td>
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-
                    <td>Prhl(RL)_CmR_LasI</td>
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-
                    <td>Kohdai Hibi</td>
+
-
                    <td>1435</td>
+
-
                  </tr>
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                  <tr class="underName">
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                    <td>&nbsp;</td>
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                    <td><a href="http://parts.igem.org/Part:BBa_K1529020">K1529020</a></td>
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                    <td>Composite</td>
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-
                    <td>Ptet-ydeD-tolC</td>
+
-
                    <td>Kohdai Hibi</td>
+
-
                    <td>1633</td>
+
                   </tr>
                   </tr>
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                 </table>
                 </table>
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                 <br />
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                 <p>&nbsp;</p>
               </div>
               </div>
               <table width="900" border="0">
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                 <tr>
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                   <td colspan="2"><span class="head">Best new BioBrick part (natural and engineered): <a href="http://parts.igem.org/Part:BBa_K1139021" rel="nofollow">BBa_K</a></span></td>
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                   <td><h2>1. Best New Basic Part<span class="head">:<a href="http://parts.igem.org/Part:BBa_K1529301"> BBa_K1529301</a>, <a href="http://parts.igem.org/Part:BBa_K1529311">BBa_K1529311</a>, <a href="http://parts.igem.org/Part:BBa_K1529321">BBa_K1529321</a></span></h2></td>
                 </tr>
                 </tr>
                 <tr>
                 <tr>
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                   <td width="561">&nbsp;</td>
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                   <td><p class="head">&#8226; Prhl(RL)-GFP  (<a href="http://parts.igem.org/Part:BBa_K1529301">BBa_K1529301</a><a href="http://parts.igem.org/Part:BBa_K1529300"></a>)</p></td>
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                  <td width="329">&nbsp;</td>
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                 </tr>
                 </tr>
                 <tr>
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                   <td>Informations</td>
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                   <td><p class="info-18">Prhl(RL) is a promoter that is activated by C4HSL-RhlR complex.&nbsp;<br />
-
                  <td><img src="http://cyberd.org/img/3/Under-Construction-Anime.jpg" height="300" /></td>
+
                    We improved Prhl promoter (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0071">BBa_R0071</a>) by changing LuxR binding site of Plux promoter (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0062">BBa_R0062</a>) to RhlR binding site. <br />
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                </tr>
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                    To characterize the function of the&nbsp;Prhl(RL) promoter (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1529300">BBa_K1529300</a>), we constructed this part,  Prhl(RL)-GFP (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1529301">BBa_K1529301</a>) , by inserting a Prhl(RL) promoter into the upstream region of GFP coding sequence . </p>
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                <tr>
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                    <p class="info-18">By using reporter cells containing Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL.&nbsp;<br />
-
                  <td>&nbsp;</td>
+
                    We confirmed that our new&nbsp;Prhl(RL)&nbsp;promoter was actually activated by C4HSL through an induction assay. (Fig. 5-1-1-1.)
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                  <td>&nbsp;</td>
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                  </p></td>
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                <tr>
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                  <td>&nbsp;</td>
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                  <td>&nbsp;</td>
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                 </tr>
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                 <tr>
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                   <td colspan="2" class="head">Best new BioBrick part (natural): <a href="http://parts.igem.org/Part:BBa_K1139020" rel="nofollow">BBa_K</a></td>
+
                   <td><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig5-1-1-1.png"><img src="https://static.igem.org/mediawiki/2014/2/20/Tokyo_Tech_Fig5-1-1-1.png" width="709" height="370" /></a></div></td>
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                 </tr>
                 <tr>
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                   <td width="445">&nbsp;</td>
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                   <td><div align="center"><strong>Fig. 5-1-1-1.</strong> Fluorescence intensity detected by flow cytometer </div></td>
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                  <td width="445">&nbsp;</td>
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                 </tr>
                 </tr>
                 <tr>
                 <tr>
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                  <td colspan="2">Informations</td>
 
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                  <td colspan="2">&nbsp;</td>
 
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                  <td><div align="center"><img src="http://cyberd.org/img/3/Under-Construction-Anime.jpg" height="300" /></div></td>
 
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                  <td><div align="center"><img src="http://cyberd.org/img/3/Under-Construction-Anime.jpg" height="300" /></div></td>
 
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                  <td><div align="center"></div></td>
 
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                </tr>
 
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                  <td>&nbsp;</td>
 
                   <td>&nbsp;</td>
                   <td>&nbsp;</td>
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                   <td colspan="2"><span class="head">Best improved part and Best new BioBrick part (natural): <a href="http://parts.igem.org/Part:BBa_K1139201" rel="nofollow">BBa_K</a></span> </td>
+
                   <td><h2>2. Best New Composite Part<span class="head">: <a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a>, <a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a></span></h2></td>
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              <td><p class="head">&#8226;Prhl(RL)-CmR-LasI  (<a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a>)</p></td>
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                  <td colspan="2">&nbsp;</td>
+
                 </tr>
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                   <td colspan="2">Informations</td>
+
                   <td><p class="info-18">We constructed this part by combining &nbsp;<a href="http://parts.igem.org/Part:BBa_K1529300">BBa_K1529300</a>,&nbsp;<a href="http://parts.igem.org/Part:BBa_K395160">BBa_K395160</a>,&nbsp;<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>&nbsp;and&nbsp;<a href="http://parts.igem.org/Part:BBa_C0078">BBa_C0078</a>.&nbsp;Prhl(RL) promoter has no leakage and higher expression than Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>). CmR and LasI are inserted into the downstream of the promoter</p>
 +
                    <p class="info-18"This is the first BioBrick part that succeeded in C4HSL dependent CmR and 3OC12HSL production. Using this part with the plasmid that is constitutively expressing RhlR, we succeeded in confirming RhlR activates the expression of CmR and LasI in the presence of C4HSL. </p>
 +
                    <p class="info-18">
 +
To confirm CmR production, we added chloramphenicol into the medium containing Prhl(RL)-CmR-LasI cell and measured optical density for about 8 h to estimate the concentration of the cell. (Fig. 5-1-2-1.)
 +
</p>
 +
                  <p class="info-18"> Moreover, by co-culturing the cells containing this part with the cells containing 3OC12HSL dependent C4HSL producer part (<a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a>), we succeeded in constructing a positive feedback system.                    </p></td>
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                   <td colspan="2">&nbsp;</td>
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                   <td><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig5-1-2-1.png"><img src="https://static.igem.org/mediawiki/2014/1/1d/Tokyo_Tech_Fig5-1-2-1.png" height="400" /></a></div></td>
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                   <td><div align="center"><img src="http://cyberd.org/img/3/Under-Construction-Anime.jpg" height="300" /></div></td>
+
                   <td><div align="center"> <strong>Fig. 5-1-2-1. </strong>C4HSL-Dependent CmR Expression Assay</div>                     <div align="center"></div></td>
-
                  <td><div align="center"><img src="http://cyberd.org/img/3/Under-Construction-Anime.jpg" height="300" /></div></td>
+
                 </tr>
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                  <td><div align="center"></div></td>
 
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                  <td><div align="center"></div></td>
 
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                </tr>
 
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                <tr>
 
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                  <td>&nbsp;</td>
 
                   <td>&nbsp;</td>
                   <td>&nbsp;</td>
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                   <td colspan="2" class="head">Best new BioBrick part (engineered): <a href="http://parts.igem.org/Part:BBa_K1139110" rel="nofollow">BBa_K</a></td>
+
                 
 +
 
 +
<tr>
 +
                   <td><p class="head">&#8226; Plux-CmR-RhlI (<a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a>)</p></td>
                 </tr>
                 </tr>
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                   <td colspan="2">&nbsp;</td>
+
                   <td><p class="info-18">We constructed this part by combining&nbsp;<a href="http://parts.igem.org/Part:BBa_K395162">BBa_K395162</a>,&nbsp;<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>&nbsp;and&nbsp;<a href="http://parts.igem.org/Part:BBa_C0070">BBa_C0070</a>. (CmR is Chloramphenicol resistance.) <br />
 +
                  This is the first BioBrick part that succeeded in 3OC12HSL dependent CmR and C4HSL production. In our experiment, we used 3OC12HSL instead of 3OC6HSL as an inducer in order to circumvent crosstalk. Using this part with the plasmid that is constitutively expressing LuxR, we succeeded in confirming LuxR activates the expression of CmR and RhlI in the presence of 3OC12HSL. </p>
 +
                  <p class="info-18">To confirm C4HSL production, we measured the expression of GFP in reporter cells by flow cytometer. (Fig. 5-1-2-2).</p></td>
                 </tr>
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                   <td colspan="2">Informations</td>
+
                   <td><div align="center"></div>
 +
                      <div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig5-1-2-2.png"><img src="https://static.igem.org/mediawiki/2014/e/e6/Tokyo_Tech_Fig5-1-2-2.png" height="400" /></a></div></td>
                 </tr>
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                   <td colspan="2">&nbsp;</td>
+
                   <td><div align="center"><strong>Fig. 5-1-2-2.</strong> 3OC12HSL-Dependent C4HSL Production Assay</div>
 +
                      <div align="center"></div></td>
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                   <td colspan="2" class="entry-long">&nbsp;</td>
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                   <td>&nbsp;</td>
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                <tr>
+
                   <td><h2>3. Best  Part Collection: <span class="head"><a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a>, <a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a><a href="http://parts.igem.org/Part:BBa_K1139201" rel="nofollow"></a></span> </h2></td>
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                   <td colspan="2">&nbsp;</td>
+
                 </tr>
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                   <td colspan="2"><span class="head" id="Best_Part_Collection">Best Part Collection</span></td>
+
                   <td><p class="info-18">Using these parts enable us to accomplish mutualism. The results of the co-culture assay is shown in Fig. 5-1-2-3. Company’s characteristics are C4HSL-dependent survival and 3OC12HSL production (<a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a>), and Customer’s characteristics are the opposite from Company’s (<a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a>). From these characteristics, the symbiosis between the two cells can be established.</p></td>
                 </tr>
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                  <tr>
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                   <td colspan="2">&nbsp;</td>
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                   <td><p class="info-18">Two types of fluorescent proteins were used to trace the growth of each cells in our symbiosis experiments. We constructed the Company cell containing GFP and Customer cell containing RFP. By measuring the OD of the cells expressing GFP with flow cytometer, the symbiosis was detected.</p><td>
 +
                  </tr>
 +
                  <tr>
 +
                  <td><p class="info-18">Using these parts enable us to accomplish mutualism. The results of the co-culture assay is shown in Fig. 5-1-2-3. Company’s By looking at the fluorescence intensity of GFP in Company cells, the optical density increased faster  in co-culture than single culture.From this point, we can say that Company and Customer actually mutualize in the medium.
 +
</p></td>
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                   <td colspan="2" class="underName"><a href="http://parts.igem.org/Part:BBa_K1139110" rel="nofollow">BBa_K</a>,<a href="http://parts.igem.org/Part:BBa_K1139110" rel="nofollow">BBa_K</a>,<a href="http://parts.igem.org/Part:BBa_K1139110" rel="nofollow">BBa_K</a><a href="http://parts.igem.org/Part:BBa_K1139110" rel="nofollow">BBa_K</a></td>
+
                   <td><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig5-1-2-3.png"><img src="https://static.igem.org/mediawiki/2014/0/08/Tokyo_Tech_Fig5-1-2-3.png" height="400" /></a></div></td>
                 </tr>
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                   <td colspan="2">&nbsp;</td>
+
                   <td><div align="center"><strong>Fig. 5-1-2-3.</strong> The result of co-culture assay </div></td>
                 </tr>
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                   <td colspan="2">Informations</td>
+
                   <td>&nbsp;</td>
                 </tr>
                 </tr>
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                   <td colspan="2">&nbsp;</td>
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                   <td>&nbsp;</td>
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                   <td colspan="2"><div align="center"><img src="http://cyberd.org/img/3/Under-Construction-Anime.jpg" height="300" /></div></td>
+
                   <td>&nbsp;</td>
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                  <td colspan="2">&nbsp;</td>
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                 </tr>
               </table>
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</div>
+
<!--              <h2>Our submitted parts</h2>
 +
              <p>&nbsp;</p>
 +
 
 +
              <div align="center">
 +
<div style="min-height:100px;width:700px;" id="groupparts">
 +
<style>
 +
  .status_cell { width:8px; }
 +
  .status_star { width:2em; }
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 +
.c4 { width: 80px; white-space:normal; }
 +
.c6 { width: 150px; white-space:normal; }
 +
.c7 { width: 50px; white-space:normal; }
 +
  </style>
 +
<table cellspacing="0" cellpadding="0" style="width: 100%;" class=" pgrouptable tablesorter">
 +
<thead>
 +
<tr><th style="width:8px" class="header"></th><th style="width:8px" class="header"></th><th style="width:8px" class="header"></th><th style="width: 87px;" class="header">Name</th><th colspan="" style="width: 80px" class="header">Type</th><th colspan="" style="width: auto;" class="header">Description</th><th colspan="" style="width: 150px" class="header">Designer</th><th colspan="" style="width: 50px" class="header">Length</th>
 +
</tr></thead><tbody>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529000" class="noul_link part_link">BBa_K1529000</a></td><td>Reporter</td><td>YdeD</td><td width="100px">Riku Shinohara</td><td align="right">4</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529001" class="noul_link part_link">BBa_K1529001</a></td><td>Composite</td><td>rbs_ydeD</td><td width="100px">Riku Shinohara</td><td align="right">24</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529002" class="noul_link part_link">BBa_K1529002</a></td><td>Composite</td><td>Ptet_rbs_yded</td><td width="100px">Riku Shinohara</td><td align="right">86</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529003" class="noul_link part_link">BBa_K1529003</a></td><td>Composite</td><td>Plux_rbs_ydeD</td><td width="100px">Riku Shinohara</td><td align="right">87</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529010" class="noul_link part_link">BBa_K1529010</a></td><td>Reporter</td><td>yfiK</td><td width="100px">Riku Shinohara</td><td align="right">10</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529011" class="noul_link part_link">BBa_K1529011</a></td><td>Composite</td><td>rbs_yfiK</td><td width="100px">Riku Shinohara</td><td align="right">30</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529012" class="noul_link part_link">BBa_K1529012</a></td><td>Composite</td><td>Ptet_rbs_yfiK</td><td width="100px">Riku Shinohara</td><td align="right">92</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529013" class="noul_link part_link">BBa_K1529013</a></td><td>Composite</td><td>Plux_rbs_yfiK</td><td width="100px">Riku Shinohara</td><td align="right">93</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529020" class="noul_link part_link">BBa_K1529020</a></td><td>Composite</td><td>Ptet_rbs_ydeD_rbs_tolC</td><td width="100px">Riku Shinohara</td><td align="right">1633</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529021" class="noul_link part_link">BBa_K1529021</a></td><td>Composite</td><td>Plux_rbs_ydeD_rbs_tolC</td><td width="100px">Riku Shinohara</td><td align="right">1634</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529030" class="noul_link part_link">BBa_K1529030</a></td><td>Composite</td><td>Plux-M256I CysE</td><td width="100px">Naoto Takahashi</td><td align="right">902</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529080" class="noul_link part_link">BBa_K1529080</a></td><td>Composite</td><td>araC_Pbad_M256ICysE_Ptet_luxR_TT</td><td width="100px">Kohdai Hibi</td><td align="right">3056</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529100" class="noul_link part_link">BBa_K1529100</a></td><td>Composite</td><td>rbs_ppk</td><td width="100px">Riku Shinohara</td><td align="right">2070</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529101" class="noul_link part_link">BBa_K1529101</a></td><td>Composite</td><td>Ptet_rbs_ppk1_ter</td><td width="100px">Riku Shinohara</td><td align="right">162</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529102" class="noul_link part_link">BBa_K1529102</a></td><td>Composite</td><td>Ptet_rbs_ppk1_ter</td><td width="100px">Riku Shinohara</td><td align="right">157</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529103" class="noul_link part_link">BBa_K1529103</a></td><td>Composite</td><td>Plac_rbs_ppx_rbs_ppk1</td><td width="100px">Shoko Suzuki</td><td align="right">2355</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529104" class="noul_link part_link">BBa_K1529104</a></td><td>Composite</td><td>PcIλ_rbs_ackA_rbs_ppk1</td><td width="100px">Shoko Suzuki</td><td align="right">3356</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529105" class="noul_link part_link">BBa_K1529105</a></td><td>Composite</td><td>Pt7_rbs_ppk1_rbs_ppx</td><td width="100px">Shoko Suzuki</td><td align="right">2167</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529106" class="noul_link part_link">BBa_K1529106</a></td><td>Composite</td><td>Pt7_rbs_ppk1_rbs_AckA</td><td width="100px">Shoko Suzuki</td><td align="right">3319</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529110" class="noul_link part_link">BBa_K1529110</a></td><td>Coding</td><td>ppx</td><td width="100px">Riku Shinohara</td><td align="right">1542</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529111" class="noul_link part_link">BBa_K1529111</a></td><td>Composite</td><td>rbs_ppx</td><td width="100px">Riku Shinohara</td><td align="right">69</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529112" class="noul_link part_link">BBa_K1529112</a></td><td>Composite</td><td>Ptet_rbs_ppx_ter</td><td width="100px">Riku Shinohara</td><td align="right">131</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529113" class="noul_link part_link">BBa_K1529113</a></td><td>Composite</td><td>Pc1λ_rbs_ppx_ter</td><td width="100px">Riku Shinohara</td><td align="right">277</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529120" class="noul_link part_link">BBa_K1529120</a></td><td>Composite</td><td>phoU</td><td width="100px">Riku Shinohara</td><td align="right">726</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529121" class="noul_link part_link">BBa_K1529121</a></td><td>Composite</td><td>rbs_phoU</td><td width="100px">Riku Shinohara</td><td align="right">24</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529122" class="noul_link part_link">BBa_K1529122</a></td><td>Composite</td><td>Ptet_rbs_phoU</td><td width="100px">Riku Shinohara</td><td align="right">86</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529123" class="noul_link part_link">BBa_K1529123</a></td><td>Composite</td><td>Plac_rbs_phoU</td><td width="100px">Riku Shinohara</td><td align="right">232</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529130" class="noul_link part_link">BBa_K1529130</a></td><td>Composite</td><td>rbs_ackA</td><td width="100px">Riku Shinohara</td><td align="right">1221</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529131" class="noul_link part_link">BBa_K1529131</a></td><td>Composite</td><td>Pc1λ_rbs_ackA</td><td width="100px">Riku Shinohara</td><td align="right">1278</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529150" class="noul_link part_link">BBa_K1529150</a></td><td>Composite</td><td>PT7_obcA</td><td width="100px">Shoko Suzuki</td><td align="right">1827</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529151" class="noul_link part_link">BBa_K1529151</a></td><td>Composite</td><td>PT7_obcB</td><td width="100px">Shoko Suzuki</td><td align="right">763</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529199" class="noul_link part_link">BBa_K1529199</a></td><td>Composite</td><td> -- No description -- </td><td width="100px">Riku Shinohara</td><td align="right">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529200" class="noul_link part_link">BBa_K1529200</a></td><td>Composite</td><td>Ptet_tolC</td><td width="100px">Kohdai Hibi</td><td align="right">1601</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529201" class="noul_link part_link">BBa_K1529201</a></td><td>Composite</td><td>rbs_ydeD_rbs_tolC</td><td width="100px">Kohdai Hibi</td><td align="right">1571</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529202" class="noul_link part_link">BBa_K1529202</a></td><td>Composite</td><td>Plux_tolC</td><td width="100px">Kohdai Hibi</td><td align="right">1602</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529230" class="noul_link part_link">BBa_K1529230</a></td><td>Composite</td><td>Ptet_M256ICysE</td><td width="100px">Kohdai Hibi</td><td align="right">901</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529235" class="noul_link part_link">BBa_K1529235</a></td><td>Composite</td><td>Plux_M256ICysE_Ptet_luxR_TT</td><td width="100px">Kohdai Hibi</td><td align="right">1908</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529240" class="noul_link part_link">BBa_K1529240</a></td><td>Composite</td><td>rbs_aiiA</td><td width="100px">Kohdai Hibi</td><td align="right">832</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529241" class="noul_link part_link">BBa_K1529241</a></td><td>Composite</td><td>Plac_cIλ_aiiA</td><td width="100px">Kohdai Hibi</td><td align="right">1841</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529242" class="noul_link part_link">BBa_K1529242</a></td><td>Composite</td><td>PcIλ_lacI_luxI</td><td width="100px">Kohdai Hibi</td><td align="right">1897</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529243" class="noul_link part_link">BBa_K1529243</a></td><td>Composite</td><td>Ptet_luxI</td><td width="100px">Kohdai Hibi</td><td align="right">723</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529250" class="noul_link part_link">BBa_K1529250</a></td><td>Composite</td><td>Plac_rbs</td><td width="100px">Kohdai Hibi</td><td align="right">220</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529251" class="noul_link part_link">BBa_K1529251</a></td><td>Composite</td><td>rbs_RhlI</td><td width="100px">Kohdai Hibi</td><td align="right">685</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529252" class="noul_link part_link">BBa_K1529252</a></td><td>Composite</td><td>Ptet_RhlI</td><td width="100px">Kohdai Hibi</td><td align="right">747</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529263" class="noul_link part_link">BBa_K1529263</a></td><td>Composite</td><td>Plux_CmR_LasI</td><td width="100px">Kohdai Hibi</td><td align="right">1435</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529264" class="noul_link part_link">BBa_K1529264</a></td><td>Composite</td><td>Plux_CmR_luxI</td><td width="100px">Kohdai Hibi</td><td align="right">1411</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529265" class="noul_link part_link">BBa_K1529265</a></td><td>Composite</td><td>Plux_CmR_RhlI</td><td width="100px">Kohdai Hibi</td><td align="right">1435</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529267" class="noul_link part_link">BBa_K1529267</a></td><td>Composite</td><td>Ptet_CmR</td><td width="100px">Kohdai Hibi</td><td align="right">741</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529268" class="noul_link part_link">BBa_K1529268</a></td><td>Composite</td><td>Ptet_CmR_luxI</td><td width="100px">Kohdai Hibi</td><td align="right">1410</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529270" class="noul_link part_link">BBa_K1529270</a></td><td>Composite</td><td>Ptet_RhlR</td><td width="100px">Kohdai Hibi</td><td align="right">867</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529271" class="noul_link part_link">BBa_K1529271</a></td><td>Composite</td><td>Ptet_RhlR_luxI</td><td width="100px">Kohdai Hibi</td><td align="right">1536</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529290" class="noul_link part_link">BBa_K1529290</a></td><td>Composite</td><td>Plux/lac_luxI</td><td width="100px">Kohdai Hibi</td><td align="right">743</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529291" class="noul_link part_link">BBa_K1529291</a></td><td>Composite</td><td>Plux/lac_aiiA</td><td width="100px">Kohdai Hibi</td><td align="right">914</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529292" class="noul_link part_link">BBa_K1529292</a></td><td>Composite</td><td>Plux_luxI</td><td width="100px">Riku Shinohara</td><td align="right">724</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529293" class="noul_link part_link">BBa_K1529293</a></td><td>Composite</td><td>Plux_aiiA</td><td width="100px">Kohdai Hibi</td><td align="right">895</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529300" class="noul_link part_link">BBa_K1529300</a></td><td>Regulatory</td><td>Prhl(RL)</td><td width="100px">Kohdai Hibi</td><td align="right">55</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529301" class="noul_link part_link">BBa_K1529301</a></td><td>Composite</td><td>Prhl(RL)-GFP</td><td width="100px">Kohdai Hibi</td><td align="right">990</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529302" class="noul_link part_link">BBa_K1529302</a></td><td>Composite</td><td>Prhl(RL)_CmR_LasI</td><td width="100px">Kohdai Hibi</td><td align="right">1435</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529310" class="noul_link part_link">BBa_K1529310</a></td><td>Regulatory</td><td>Prhl(LR)</td><td width="100px">Kohdai Hibi</td><td align="right">55</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529311" class="noul_link part_link">BBa_K1529311</a></td><td>Composite</td><td>Prhl(LR)-GFP</td><td width="100px">Kohdai Hibi</td><td align="right">990</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529320" class="noul_link part_link">BBa_K1529320</a></td><td>Regulatory</td><td>Prhl(RR)</td><td width="100px">Kohdai Hibi</td><td align="right">55</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529321" class="noul_link part_link">BBa_K1529321</a></td><td>Composite</td><td>Prhl(RR)-GFP</td><td width="100px">Kohdai Hibi</td><td align="right">990</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529430" class="noul_link part_link">BBa_K1529430</a></td><td>Composite</td><td>PcIλ_lacI</td><td width="100px">Shoko Suzuki</td><td align="right">1141</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529431" class="noul_link part_link">BBa_K1529431</a></td><td>Composite</td><td>Plac_cIλ</td><td width="100px">Shoko Suzuki</td><td align="right">981</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529432" class="noul_link part_link">BBa_K1529432</a></td><td>Composite</td><td>Plux_cIλ</td><td width="100px">Shoko Suzuki</td><td align="right">836</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529567" class="noul_link part_link">BBa_K1529567</a></td><td>Coding</td><td>parts</td><td width="100px">Shoko Suzuki</td><td align="right">51</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529568" class="noul_link part_link">BBa_K1529568</a></td><td>Composite</td><td>sss</td><td width="100px">Shoko Suzuki</td><td align="right">967</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529777" class="noul_link part_link">BBa_K1529777</a></td><td>Composite</td><td>Plux_CmR_luxI</td><td width="100px">Riku Shinohara</td><td align="right">1411</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529787" class="noul_link part_link">BBa_K1529787</a></td><td>Reporter</td><td>abe</td><td width="100px">Gyomon Ryu</td><td align="right">&nbsp;</td>
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</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529789" class="noul_link part_link">BBa_K1529789</a></td><td>Coding</td><td>abe</td><td width="100px">Gyomon Ryu</td><td align="right">&nbsp;</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529797" class="noul_link part_link">BBa_K1529797</a></td><td>Composite</td><td>Plux_CmR_RhlI</td><td width="100px">Shoko Suzuki</td><td align="right">1435</td>
 +
</tr>
 +
<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529923" class="noul_link part_link">BBa_K1529923</a></td><td>Terminator</td><td>lksj</td><td width="100px">Kohdai Hibi</td><td align="right">18</td>
 +
</tr>
 +
<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529948" class="noul_link part_link">BBa_K1529948</a></td><td>RBS</td><td>abe</td><td width="100px">Gyomon Ryu</td><td align="right">24</td>
 +
</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529950" class="noul_link part_link">BBa_K1529950</a></td><td>Terminator</td><td>1</td><td width="100px">Xinan Wang</td><td align="right">&nbsp;</td>
 +
</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529951" class="noul_link part_link">BBa_K1529951</a></td><td>Composite</td><td>1</td><td width="100px">Daisuke Kiga</td><td align="right">967</td>
 +
</tr>
 +
<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529952" class="noul_link part_link">BBa_K1529952</a></td><td>Composite</td><td>1</td><td width="100px">Xinan Wang</td><td align="right">967</td>
 +
</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529953" class="noul_link part_link">BBa_K1529953</a></td><td>Generator</td><td>K1139200 B0030 E0040 B0010 B0012</td><td width="100px">Gyomon Ryu</td><td align="right">967</td>
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</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529955" class="noul_link part_link">BBa_K1529955</a></td><td>Composite</td><td>1</td><td width="100px">Ayaka Murase</td><td align="right">967</td>
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</tr>
 +
<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529958" class="noul_link part_link">BBa_K1529958</a></td><td>Composite</td><td>1</td><td width="100px">Naoto Takahashi</td><td align="right">967</td>
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</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529959" class="noul_link part_link">BBa_K1529959</a></td><td>Regulatory</td><td>1</td><td width="100px">Xinan Wang</td><td align="right">&nbsp;</td>
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</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529972" class="noul_link part_link">BBa_K1529972</a></td><td>Composite</td><td>oxalic acid</td><td width="100px">Matsumura Keina</td><td align="right">967</td>
 +
</tr>
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<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529973" class="noul_link part_link">BBa_K1529973</a></td><td>Coding</td><td>zen</td><td width="100px">Matsumura Keina</td><td align="right">&nbsp;</td>
 +
</tr>
 +
<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529978" class="noul_link part_link">BBa_K1529978</a></td><td>Regulatory</td><td>abe</td><td width="100px">Daisuke Kiga</td><td align="right">55</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529987" class="noul_link part_link">BBa_K1529987</a></td><td>Regulatory</td><td>1</td><td width="100px">Naoto Takahashi</td><td align="right">92</td>
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</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529996" class="noul_link part_link">BBa_K1529996</a></td><td>Composite</td><td>1</td><td width="100px">Kino Yuta</td><td align="right">132</td>
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</tr>
 +
<tr>
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<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529997" class="noul_link part_link">BBa_K1529997</a></td><td>RBS</td><td>aa</td><td width="100px">Kino Yuta</td><td align="right">12</td>
 +
</tr>
 +
<tr>
 +
<td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;</td><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1529998" class="noul_link part_link">BBa_K1529998</a></td><td>Terminator</td><td>1</td><td width="100px">Ayaka Murase</td><td align="right">20</td>
 +
</tr>
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</tbody>
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</table></div>
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Latest revision as of 03:58, 18 October 2014

Tokyo_Tech

Parts

Each part gives us a whole new experience

 

Favorite Tokyo Tech 2014 iGEM Team Parts

 
Name
Type
Description
Design
Length (bp)
  BBa_K1529301 Composite Prhl(RL)-GFP Kohdai Hibi 990
  BBa_K1529302 Composite Prhl(RL)-CmR-LasI Kohdai Hibi 1435
  BBa_K1529797 Composite Plux-CmR-RhlI Shoko Suzuki 1435

 

Tokyo Tech 2014 iGEM Team Parts

 
Name
Type
Description
Design
Length (bp)
  BBa_K1529311 Composite Prhl(LR)-GFP Kohdai Hibi 990
  BBa_K1529321 Composite Prhl(RR)-GFP Kohdai Hibi 990

 

1. Best New Basic Part: BBa_K1529301, BBa_K1529311, BBa_K1529321

• Prhl(RL)-GFP (BBa_K1529301)

Prhl(RL) is a promoter that is activated by C4HSL-RhlR complex. 
We improved Prhl promoter (BBa_R0071) by changing LuxR binding site of Plux promoter (BBa_R0062) to RhlR binding site.
To characterize the function of the Prhl(RL) promoter (BBa_K1529300), we constructed this part,  Prhl(RL)-GFP (BBa_K1529301) , by inserting a Prhl(RL) promoter into the upstream region of GFP coding sequence .

By using reporter cells containing Prhl(RL)-GFP, we measured the fluorescence intensity of the cells induced by C4HSL. 
We confirmed that our new Prhl(RL) promoter was actually activated by C4HSL through an induction assay. (Fig. 5-1-1-1.)

Fig. 5-1-1-1. Fluorescence intensity detected by flow cytometer
 

2. Best New Composite Part: BBa_K1529302, BBa_K1529797

•Prhl(RL)-CmR-LasI (BBa_K1529302)

We constructed this part by combining  BBa_K1529300BBa_K395160BBa_B0034 and BBa_C0078. Prhl(RL) promoter has no leakage and higher expression than Prhl promoter (BBa_R0071). CmR and LasI are inserted into the downstream of the promoter

To confirm CmR production, we added chloramphenicol into the medium containing Prhl(RL)-CmR-LasI cell and measured optical density for about 8 h to estimate the concentration of the cell. (Fig. 5-1-2-1.)

Moreover, by co-culturing the cells containing this part with the cells containing 3OC12HSL dependent C4HSL producer part (BBa_K1529797), we succeeded in constructing a positive feedback system.

Fig. 5-1-2-1. C4HSL-Dependent CmR Expression Assay
 

• Plux-CmR-RhlI (BBa_K1529797)

We constructed this part by combining BBa_K395162BBa_B0034 and BBa_C0070. (CmR is Chloramphenicol resistance.)
This is the first BioBrick part that succeeded in 3OC12HSL dependent CmR and C4HSL production. In our experiment, we used 3OC12HSL instead of 3OC6HSL as an inducer in order to circumvent crosstalk. Using this part with the plasmid that is constitutively expressing LuxR, we succeeded in confirming LuxR activates the expression of CmR and RhlI in the presence of 3OC12HSL.

To confirm C4HSL production, we measured the expression of GFP in reporter cells by flow cytometer. (Fig. 5-1-2-2).

Fig. 5-1-2-2. 3OC12HSL-Dependent C4HSL Production Assay
 

3. Best Part Collection: BBa_K1529302, BBa_K1529797

Using these parts enable us to accomplish mutualism. The results of the co-culture assay is shown in Fig. 5-1-2-3. Company’s characteristics are C4HSL-dependent survival and 3OC12HSL production (BBa_K1529302), and Customer’s characteristics are the opposite from Company’s (BBa_K1529797). From these characteristics, the symbiosis between the two cells can be established.

Two types of fluorescent proteins were used to trace the growth of each cells in our symbiosis experiments. We constructed the Company cell containing GFP and Customer cell containing RFP. By measuring the OD of the cells expressing GFP with flow cytometer, the symbiosis was detected.

Using these parts enable us to accomplish mutualism. The results of the co-culture assay is shown in Fig. 5-1-2-3. Company’s By looking at the fluorescence intensity of GFP in Company cells, the optical density increased faster in co-culture than single culture.From this point, we can say that Company and Customer actually mutualize in the medium.

Fig. 5-1-2-3. The result of co-culture assay