Team:Goettingen/protocol peptide

From 2014.igem.org

(Difference between revisions)
m
(Blanked the page)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Goettingen/header}}
+
 
-
{{:Team:Goettingen/proLP}}
+
-
<html>
+
-
<div class="proRP">
+
-
        <h3 id="construction">Peptide Library Construction</h3>
+
-
            <p>Starting with GFP scaffolds (Denoted pRS 316-GFPM), random loop libraries were created. Randomized  amino acids were inserted at Asp-102/Asp-103 and Glu-172/Asp-173 by using the NNK method in order to create the random loop libraries. NNK sequences in primers number 13 and 16 were used to create regions I and ӀӀӀ. After having randomized DNA sequences, these two regions were assembled with region ӀӀ which remained intact. In each of the amplification and extension steps, <a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>Pfus</i> PCR protocol</a> was used. For the assembly of the three regions, an <a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Asse_PCR">assembly PCR</a> was used. <br /><br />
+
-
            </p>
+
-
        <center><img src="https://static.igem.org/mediawiki/2014/d/d3/Goettingen_peptide_library.png" width="350px"/></center><br />
+
-
        <p style="text-align:center"><b>Figure. Peptide Library Construction</b></p>
+
-
        </div>
+
-
<div id="footerbanner"><a href="https://2014.igem.org/Team:Goettingen/team_sponsors"><img src="https://static.igem.org/mediawiki/2014/7/7a/Goettingen_footer_banner.png" /></a></div>
+
-
</html>
+

Latest revision as of 21:18, 15 October 2014

Retrieved from "http://2014.igem.org/Team:Goettingen/protocol_peptide"