Team:Goettingen/protocol peptide

From 2014.igem.org

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<div class="proRP">
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        <h3 id="construction">Peptide Library Construction</h3>
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            <p>Starting with GFP scaffolds (Denoted pRS 316-GFPM), random loop libraries were created. Randomized  amino acids were inserted at Asp-102/Asp-103 and Glu-172/Asp-173 by using the NNK method in order to create the random loop libraries. NNK sequences in primers number 13 and 16 were used to create regions I and ӀӀӀ. After having randomized DNA sequences, these two regions were assembled with region ӀӀ which remained intact. In each of the amplification and extension steps, <a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>Pfus</i> PCR protocol</a> was used. For the assembly of the three regions, an assembly PCR was used. <br /><br />
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            </p>
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        <center><img src="https://static.igem.org/mediawiki/2014/d/d3/Goettingen_peptide_library.png" width="400px"/></center><br />
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        <p style="text-align:center"><b>Figure. Peptide Library Construction</b></p>
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        </div>
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<div id="footerbanner"><a href="https://2014.igem.org/Team:Goettingen/team_sponsors"><img src="https://static.igem.org/mediawiki/2014/7/7a/Goettingen_footer_banner.png" /></a></div>
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</html>
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Latest revision as of 21:18, 15 October 2014

Retrieved from "http://2014.igem.org/Team:Goettingen/protocol_peptide"