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Team:Imperial/Project
From 2014.igem.org
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<h2>Introduction</h2> | <h2>Introduction</h2> | ||
| - | <p>This summer we | + | <p>This summer we investigated the exciting biomaterial bacterial cellulose. In our project we optimise the production of bacterial cellulose by engineering <em>Gluconacetobacter xylinus</em> and transferring the system into <em>E. coli</em>. We also explore processing of our synthetic biology material, producing and testing water filters. To improve our material's performance for this application we functionalise our cellulose with binding proteins to trap specific contaminants. |
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<h2>Background</h2> | <h2>Background</h2> | ||
<p>Cellulose is the most abundant organic polymer found in nature. Due to its versatility and ubiquity we find cellulose has applications in areas from medicine to textiles.Much of the cellulose we use is impure as it is derived from plants. Bacteria offer an alternative means of production that produces a cellulose that is purer and requires less processing.</p> | <p>Cellulose is the most abundant organic polymer found in nature. Due to its versatility and ubiquity we find cellulose has applications in areas from medicine to textiles.Much of the cellulose we use is impure as it is derived from plants. Bacteria offer an alternative means of production that produces a cellulose that is purer and requires less processing.</p> | ||
| - | <div class="more-box more-box-bottom"><a href=" | + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/Project_Background">read more...</a> |
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| - | < | + | <h2><em>G. xylinus</em></h2> |
| + | <p>Cellulose is naturally produced by bacteria of several genera as an extracellular matrix. This functions as a protective mechanism, shielding the bacteria from the environment. The gram-negative <em>Gluconacetobacter xylinus</em> (formerly <em>Acetobacter xylinum</em>) is a high-yielding producer of bacterial cellulose and so served as a suitable base for further optimisation.</p> | ||
| + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/Gluconacetobacter">read more...</a> | ||
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| - | <h2> | + | <h2><em>E. coli</em></h2> |
| - | <p> | + | <p>While <em>Gluconacetobacter</em> is a high producer of bacterial cellulose, <em>E. coli</em> is currently a more robust host for synthetic biology. Transferring the bacterial cellulose operon into <em>E. coli</em> would ease further in-vivo modification of the cellulose by allowing well characterised parts to be used more directly and has the potential for higher productivity. |
| - | <div class="more-box more-box-bottom"><a href=" | + | </p> |
| + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/EColi">read more...</a> | ||
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| - | <h2> | + | <h2>Co-culturing</h2> |
| - | <p> | + | <p>The idea of combining E. coli as an efficient cloning organism since it has the largest library of well characterised parts available and G. xylinus as a robust efficient cellulose producing host came about as a way to take advantage of the characteristics of each host.</p> |
| - | </p> | + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/coculture">read more...</a> |
| - | <div class="more-box more-box-bottom"><a href=" | + | |
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<h2>Functionalisation</h2> | <h2>Functionalisation</h2> | ||
<p>Pure bacterial cellulose is itself a useful biomaterial with material properties that facilitate applications from filtration to wound dressing. We modify the material, chemical and biological properties of our biomaterial through the addition of functional proteins. We investigated different methods of coupling these to the cellulose.</p> | <p>Pure bacterial cellulose is itself a useful biomaterial with material properties that facilitate applications from filtration to wound dressing. We modify the material, chemical and biological properties of our biomaterial through the addition of functional proteins. We investigated different methods of coupling these to the cellulose.</p> | ||
| - | <div class="more-box more-box-bottom"><a href=" | + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/Functionalisation">read more...</a> |
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<div class="content content-single"> | <div class="content content-single"> | ||
| - | < | + | <h2>Water Filtration</h2> |
| + | <p>Our mass produced and functionalised cellulose can be used for a range of exciting applications. The biological functionalisation allows our material to perform enzymatic actions on its environment. We targeted our functionalisation to the problem of water treatment and filtration.</p> | ||
| + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/Water_Filtration">read more...</a> | ||
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| - | <h2> | + | <h2>Mass Production and Processing</h2> |
| - | <p> | + | <p>To produce a useable material from the wet pellicle we grew up cellulose in bulk in order to experiment with various methods of treating and processing it.This produced materials with a range of properties.</p> |
| - | <div class="more-box more-box-bottom"><a href=" | + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/Mass_Production_and_Processing">read more...</a> |
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| - | <h2> | + | <h2>Tensile Testing</h2> |
| - | <p> | + | <p> Having produced large quantities of bacterial cellulose, it is key to quantify the quality of our biomaterial. This determines the water flow rates that can be expected in a membrane bioreactor water filtration setup.</p> |
| - | <div class="more-box more-box-bottom"><a href=" | + | <div class="more-box more-box-bottom"><a href="https://2014.igem.org/Team:Imperial/Mechanical_Testing">read more...</a> |
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Latest revision as of 02:01, 18 October 2014
Project
Introduction
This summer we investigated the exciting biomaterial bacterial cellulose. In our project we optimise the production of bacterial cellulose by engineering Gluconacetobacter xylinus and transferring the system into E. coli. We also explore processing of our synthetic biology material, producing and testing water filters. To improve our material's performance for this application we functionalise our cellulose with binding proteins to trap specific contaminants.
Background
Cellulose is the most abundant organic polymer found in nature. Due to its versatility and ubiquity we find cellulose has applications in areas from medicine to textiles.Much of the cellulose we use is impure as it is derived from plants. Bacteria offer an alternative means of production that produces a cellulose that is purer and requires less processing.
G. xylinus
Cellulose is naturally produced by bacteria of several genera as an extracellular matrix. This functions as a protective mechanism, shielding the bacteria from the environment. The gram-negative Gluconacetobacter xylinus (formerly Acetobacter xylinum) is a high-yielding producer of bacterial cellulose and so served as a suitable base for further optimisation.
E. coli
While Gluconacetobacter is a high producer of bacterial cellulose, E. coli is currently a more robust host for synthetic biology. Transferring the bacterial cellulose operon into E. coli would ease further in-vivo modification of the cellulose by allowing well characterised parts to be used more directly and has the potential for higher productivity.
Co-culturing
The idea of combining E. coli as an efficient cloning organism since it has the largest library of well characterised parts available and G. xylinus as a robust efficient cellulose producing host came about as a way to take advantage of the characteristics of each host.
Functionalisation
Pure bacterial cellulose is itself a useful biomaterial with material properties that facilitate applications from filtration to wound dressing. We modify the material, chemical and biological properties of our biomaterial through the addition of functional proteins. We investigated different methods of coupling these to the cellulose.
Water Filtration
Our mass produced and functionalised cellulose can be used for a range of exciting applications. The biological functionalisation allows our material to perform enzymatic actions on its environment. We targeted our functionalisation to the problem of water treatment and filtration.
Mass Production and Processing
To produce a useable material from the wet pellicle we grew up cellulose in bulk in order to experiment with various methods of treating and processing it.This produced materials with a range of properties.
Tensile Testing
Having produced large quantities of bacterial cellulose, it is key to quantify the quality of our biomaterial. This determines the water flow rates that can be expected in a membrane bioreactor water filtration setup.
