Team:Paris Bettencourt/Results

From 2014.igem.org

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-
<p id=top><a href="#topheader"><img src="https://static.igem.org/mediawiki/2014/d/d3/Arrowpb.png"></a></p>
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 +
<img src="https://static.igem.org/mediawiki/2014/c/c6/PB14results.png" class=nameimg>
<div id="topheader">
<div id="topheader">
</div>
</div>
-
<div id=logo><img id=logoblanc src="https://static.igem.org/mediawiki/2014/7/7d/Logoblanc.png"><img id=logorose src="https://static.igem.org/mediawiki/2014/2/28/Logorose.png"></div>
+
-
<div id=fond>
+
-
        <table id=headtable>
+
-
<tr>
+
-
<td><b>SMELL THE ROSES</b></br>
+
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Cras elementum mauris interdum lacus venenatis aliquet. In ac nulla semper, convallis orci non, vestibulum nisi. Maecenas quis ultricies tellus. Sed vulputate tempor ultrices. Donec in eleifend dui. Quisque tempor sed leo vel euismod. Quisque auctor pharetra vestibulum. </p></td>
+
-
<td><b>TEEN SPIRIT</b></br>
+
-
<p class=text1>Nunc porttitor arcu eget est pretium rutrum. Fusce feugiat ex eu odio pharetra rhoncus. Nullam tincidunt lobortis nibh. Maecenas non felis eu velit tincidunt tristique. Phasellus in urna odio. Nunc vestibulum sem justo, ac laoreet elit sodales ut. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Donec eu massa. </p></td>
+
-
<td><b>SOMETHING FISHY</b></br>
+
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc convallis fringilla nunc, nec tristique neque. Praesent ut ante ultrices, dignissim nunc eget, interdum velit. Donec lobortis iaculis lorem, eget ornare quam finibus et. Vestibulum vehicula fringilla mauris, vitae feugiat ligula pharetra a. Duis fermentum neque vel dapibus accumsan. Quisque fringilla. </p></td>
+
-
<td><b>GOODY TWO SHOES</b></br>
+
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Mauris vel neque odio. Suspendisse eget dui quis quam semper placerat at nec orci. Cras malesuada sem nec aliquam imperdiet. Cras vitae nisl eu augue iaculis aliquam et eget turpis. Fusce pulvinar ipsum eu neque auctor, at luctus nibh pulvinar. Vivamus in. </p></td>
+
-
<td><b>DON'T SWEAT IT</b></br>
+
-
<p class=text1>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Mauris vel neque odio. Suspendisse eget dui quis quam semper placerat at nec orci. Cras malesuada sem nec aliquam imperdiet. Cras vitae nisl eu augue iaculis aliquam et eget turpis. Fusce pulvinar ipsum eu neque auctor, at luctus nibh pulvinar. Vivamus in. </p></td>
+
-
</tr>
+
-
</table>
+
-
        </div>
+
<table id=tablelien>
<table id=tablelien>
<tr>
<tr>
-
<td><a href="#project1">Project 1</a></td>
+
<td><a href="#project1">Smell<br>the roses</a></td>
-
<td><a href="#project2">Project 2</a></td>
+
<td><a href="#project2">Teen<br>spirit</a></td>
-
<td><a href="#project1">Project 3</a></td>
+
<td><a href="#project3">Something<br>fishy</a></td>
-
<td><a href="#project1">Project 4</a></td>
+
<td><a href="#project4">Goody<br>two shoes</a></td>
-
<td><a href="#motivation">Motivations</a></td>
+
<td><a href="#project5">Don't<br>sweat it</a></td>
</tr>
</tr>
</table>
</table>
 +
<div class=project id=project1>
<div class=project id=project1>
-
<h6>Smell The Roses</h6>
+
 
-
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo. </p>
+
<p class=text2></br></br></br></br></p><p class=text1><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Old_People_Smell"><span class=hauteur>Teen Spirit</span></a></p>
-
<img src="">
+
 +
<p class=text1>
 +
-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.</br>
 +
-We produced the smells that compose the main odor categories perceived by humans.</br>
 +
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019 </br></p>
 +
 
 +
<p class=text1>
 +
<span class=hauteur><a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></span></br>-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. -Determined the critical smell detection limit of 2-nonenal.</br>
 +
-Analyzed 2-nonenal tolerance and degradation in natural isolates.</br></p>
</div>
</div>
 +
<p>
<div class=project id=project2>
<div class=project id=project2>
-
<h6>Teen Spirit</h6>
+
 
-
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam bibendum purus eu neque finibus, eget pellentesque sapien viverra. Vestibulum ultrices posuere tempor. Maecenas ultrices sodales magna ac placerat. Sed a ex dignissim, ornare metus non, malesuada arcu. Etiam ullamcorper odio leo, at molestie eros sollicitudin in. Morbi aliquam scelerisque facilisis. Aenean tincidunt aliquam erat, quis ullamcorper nulla accumsan ac. Proin interdum nibh quam, in lacinia ipsum dignissim at. Nunc elementum lacus sed purus pharetra tincidunt. Sed ac velit vel turpis pulvinar accumsan ut in mauris. Praesent ac dapibus dui. Nullam finibus turpis et turpis sagittis congue. </p>
+
<p class=text1></p>
-
<img src="">
+
</div>
</div>
<div class=project id=project3>
<div class=project id=project3>
-
<h6>Something Fishy</h6>
+
</br></br>
-
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed sit amet laoreet metus, ac viverra dolor. Sed et orci imperdiet sem vulputate ultricies. Aliquam erat volutpat. Cras semper ex non odio aliquet, eget feugiat eros tempor. Integer hendrerit odio et bibendum maximus. Duis scelerisque lacus in odio faucibus fringilla. Nulla eleifend aliquet molestie. Morbi aliquam rhoncus efficitur. Proin consectetur augue aliquam risus convallis egestas. Nunc viverra felis non nibh consequat, nec faucibus ipsum rutrum. Proin placerat faucibus libero vitae dapibus. </p>
+
<p class=text1>
-
<img src="">
+
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Foot_Odor">Goody Two Shoes</a></span><br>-Demonstrated that mutant strains of <i>B. subtilis</i> produce less odor.</br>
 +
-Measured growth and viability of mutants in synthetic sweat media and on socks.</br> </p>
 +
<p class=text1>
 +
<span class=hauteur><a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/TMAU">Something Fishy</a></span></br>
 +
-Cloned <i>tmm</i> into <i>E.coli</i> using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.</br>
 +
-Characterized and quantified the activity of TMM by a colorimetric assay.</br>
 +
-Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).</br>
 +
</p>
</div>
</div>
-
<div class=project id=project4>
+
<div class=project id=project4>
-
<h6>Goody Two Shoes</h6>
+
<p class=text1></p>
-
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam eu justo a dolor efficitur laoreet ut at lorem. Fusce dapibus lobortis nisi vehicula porttitor. In volutpat mauris et aliquam pellentesque. Vestibulum fringilla lacus metus, ac ullamcorper lectus sagittis sed. Suspendisse congue magna sed risus molestie aliquam. Sed placerat sagittis volutpat. Phasellus id erat neque. Quisque bibendum iaculis ante et feugiat. In hac habitasse platea dictumst. Fusce placerat lorem vel felis tincidunt, in elementum odio condimentum. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Donec tincidunt bibendum lacus non viverra. Nulla mattis, ante vitae faucibus auctor, mi purus consequat dolor, non malesuada nulla lorem ac odio. </p>
+
-
<img src="">
+
</div>
</div>
<div class=project id=project5>
<div class=project id=project5>
-
<h6>Don't Sweat It</h6>
+
<p class=text1></p>
-
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam eu justo a dolor efficitur laoreet ut at lorem. Fusce dapibus lobortis nisi vehicula porttitor. In volutpat mauris et aliquam pellentesque. Vestibulum fringilla lacus metus, ac ullamcorper lectus sagittis sed. Suspendisse congue magna sed risus molestie aliquam. Sed placerat sagittis volutpat. Phasellus id erat neque. Quisque bibendum iaculis ante et feugiat. In hac habitasse platea dictumst. Fusce placerat lorem vel felis tincidunt, in elementum odio condimentum. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Donec tincidunt bibendum lacus non viverra. Nulla mattis, ante vitae faucibus auctor, mi purus consequat dolor, non malesuada nulla lorem ac odio. </p>
+
 
-
<img src="">
+
<p class=text1>
 +
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Eliminate_Smell">Don't Sweat It</a></span><br>
 +
-Expressed the odor gene <i>agaA</i> in <i>E. coli</i> (BBa_K1403002).</br>
 +
-Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.</br>
 +
-Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.</br>
 +
-Formulated a simple skin cream encorporating live bacteria.</br></p>
</div>
</div>
 +
<div class=project id=project6>
 +
<div class=project id=project7>
 +
<p class=text1>
 +
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></span><br> -Successfully built the three devices</br>
 +
-Sequenced and gel verified the three devices</li>
 +
-Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</br>-Reported the results on the <a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</br>
 +
-Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (Device 1) </br>
 +
-Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> (Device 2) and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> (Device 3) to the Biobricks registry</br> </p>
 +
 +
 +
                     
 +
               
 +
</div>
 +
<div class=project id=project8>
 +
 +
<p class=text1>
 +
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Collaboration">Collaborations</a></span><br><b>The Smell Game</b>
 +
The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here :
 +
http://smellofus.synbio4all.org.
 +
Join us and play! </p>
 +
 +
<p class=text1>
 +
<span class=hauteur><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Human_Practice">Human practice</a></span><br><b>MOOC</b></br>
 +
-Created a MOOC with 21 lessons to help high school students build a team.</br>
 +
-More than 50 people annotated text in Genius and 15 teachers will use the tool next year.</br>
 +
-20 organizations and iGEM teams around the world have agreed to promote it.</br>
 +
-A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.</br></br>
 +
<b>Citizen science</b></br>
 +
-More than 600 citizen scientists participated.</br>
 +
-Developing a Smell game for sample analysis</br>
 +
-Living lab event in Cite de Sciences et de l'Industrie.</br>
 +
-Watching a scary movie had little to no affect on body odor intensity.</br>
 +
</br></br>
 +
 +
<b>Minder</b></br>Our answer to Tinder is Minder. You can create ideas, rate other users' ideas, view an idea's like/dislike statistics, and find additional details and contact information for ideas you like.
 +
 +
 +
</p>        </div>
 +
</body>
</body>
</html>
</html>
 +
{{:Team:Paris_Bettencourt/Footer}}

Latest revision as of 03:57, 18 October 2014

Smell
the roses		 Teen
spirit		 Something
fishy		 Goody
two shoes		 Don't
sweat it





Teen Spirit

-Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.
-We produced the smells that compose the main odor categories perceived by humans.
-BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019

Smell The Roses
-Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source. -Determined the critical smell detection limit of 2-nonenal.
-Analyzed 2-nonenal tolerance and degradation in natural isolates.



Goody Two Shoes
-Demonstrated that mutant strains of B. subtilis produce less odor.
-Measured growth and viability of mutants in synthetic sweat media and on socks.

Something Fishy
-Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
-Characterized and quantified the activity of TMM by a colorimetric assay.
-Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).

Don't Sweat It
-Expressed the odor gene agaA in E. coli (BBa_K1403002).
-Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.
-Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.
-Formulated a simple skin cream encorporating live bacteria.

Interlab study
-Successfully built the three devices
-Sequenced and gel verified the three devices -Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
-Reported the results on the Interlab Study page of the iGEM wiki
-Completed the description of the BBa_I20260 (Device 1)
-Submitted and send parts: BBa_K1403000 (Device 2) and BBa_K1403001 (Device 3) to the Biobricks registry

Collaborations
The Smell Game The web platform continues to evolve, mostly thanks to the people who give their samples and play the game. At this time, users can give a sample of his own sweat, play with the samples of others and then obtain a position of their smell.The SmellGame project relies on a web platform available here : http://smellofus.synbio4all.org. Join us and play!

Human practice
MOOC
-Created a MOOC with 21 lessons to help high school students build a team.
-More than 50 people annotated text in Genius and 15 teachers will use the tool next year.
-20 organizations and iGEM teams around the world have agreed to promote it.
-A student club was created in CRI (Paris) to continue the support for MOOC iGEM HS.

Citizen science
-More than 600 citizen scientists participated.
-Developing a Smell game for sample analysis
-Living lab event in Cite de Sciences et de l'Industrie.
-Watching a scary movie had little to no affect on body odor intensity.


Minder
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Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com
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