Team:RHIT/Safety

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<a href="https://2014.igem.org/Team:RHIT" style="text-decoration:none;color:#3B0B17">HOME </a> </td>
 
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<a href="https://2014.igem.org/Team:RHIT/Project" style="text-decoration:none;color:#3B0B17">PROJECT</a> </td>
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<a href="https://2014.igem.org/Team:RHIT/Parts" style="text-decoration:none;color:#3B0B17">PARTS</a></td>
 
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<a href="https://2014.igem.org/Team:RHIT/Team" style="text-decoration:none;color:#3B0B17">TEAM </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=RHIT" style="text-decoration:none;color:#3B0B17">OFFICIAL TEAM PROFILE </a> </td>
 
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<a href="https://2014.igem.org/Team:RHIT/Notebook" style="text-decoration:none;color:#3B0B17">NOTEBOOK </a> </td>
 
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<a href="https://2014.igem.org/Team:RHIT/Safety" style="text-decoration:none;color:#3B0B17">SAFETY </a></td>
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<tr><td > <h3> Welcome! </h3></td>
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<td > <h3> Timeline</h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3 style="font-size:36px; margin-top:17px">Safety</h3>
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<p>This past January, the Rose-Hulman iGEM team moved to its new home: a brand new synthetic biology lab located on the campus of Rose-Hulman Institute of Technology in Terre Haute, IN. Our iGEM team takes safety very seriously. This new lab is equipped with various features that ensure the safety of team members and members of the surrounding community, and protect the quality of our laboratory research. Scroll through the pictures below to see our new lab!</p>
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<h3> Edit this page!</h3>
 
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
 
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
 
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<h3> Your Lab </h3>
 
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
 
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Image:Example2_Lab_1.png|The building our lab is in!
 
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
 
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Image:Example2_Lab_4.png|Working in biosafety cabinets
 
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
 
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<a href="#" title="-80C,-20C,5C Storage "><img src="http://www.rose-hulman.edu/~bauhand/data3/tooltips/80c,20c,5c_storage_.jpg" alt="-80C,-20C,5C Storage "/>11</a>
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
 
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
 
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
 
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
 
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
 
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
 
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
 
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
 
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
 
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
 
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<p>Our laboratory space is a level two facility. It has controlled access, a biohazard disposal bin, an autoclave, a fume hood, a biosafety cabinet, an eyewash and shower station, and a fire extinguisher and fire alarm. Each team member has received laboratory safety training through previous coursework, and safety training specific to procedures used for this project was received on an as-needed basis. All team members working in the lab are required to wear proper attire, including long pants, closed-toed shoes, sleeved shirts or laboratory coats, and safety glasses, gloves, and masks when appropriate. In addition, special attention is paid to maintaining aseptic technique to minimize contamination and safety concerns. All chemicals used in the lab are stored, handled, and used as recommended by the manufacturer, and are disposed of in accordance with national, state, and local regulations and recommendations.</p>
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<p>The organisms used in our project, <i>Saccharomyces cerevisiae</i> (BY4741) and <i>Escherichia coli</i> (JW2004-1 and NEB5&#945;), are both labeled Risk Group 1 organisms by the World Health Organization in their Laboratory Biosafety Manual. This means that these organisms are low risk, and do not cause disease in healthy adult humans. All microbial strains used in the lab are either rendered antibiotic resistant by transformation, or they harbor nutritional auxotrophies or other mutations that mitigate the risk of growth outside of the laboratory or the ability of the organism to cause disease in healthy humans or animals.Furthermore, none of our recombinant constructs produce any known contagion or toxin.</p>
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Latest revision as of 13:37, 7 August 2014

SafetySlider generated by WOWSlider.com

Safety

This past January, the Rose-Hulman iGEM team moved to its new home: a brand new synthetic biology lab located on the campus of Rose-Hulman Institute of Technology in Terre Haute, IN. Our iGEM team takes safety very seriously. This new lab is equipped with various features that ensure the safety of team members and members of the surrounding community, and protect the quality of our laboratory research. Scroll through the pictures below to see our new lab!


  • Autoclave
  • biohazard bin
  • EtBr Containment
  • Hood
  • Shower and Eye Wash
  • PCR Work Station
  • Pure H2O
  • Gas Button
  • Fire Alarm
  • Fire Extinguisher
  • -80C,-20C,5C Storage
  • lab coats (1)
  • card scanner
Autoclave1 biohazard bin2 EtBr Containment 3 Hood4 Shower and Eye Wash5 PCR Work Station6 Pure H2O7 Gas Button8 Fire Alarm9 Fire Extinguisher10 -80C,-20C,5C Storage 11 lab coats (1)12 card scanner13
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Our laboratory space is a level two facility. It has controlled access, a biohazard disposal bin, an autoclave, a fume hood, a biosafety cabinet, an eyewash and shower station, and a fire extinguisher and fire alarm. Each team member has received laboratory safety training through previous coursework, and safety training specific to procedures used for this project was received on an as-needed basis. All team members working in the lab are required to wear proper attire, including long pants, closed-toed shoes, sleeved shirts or laboratory coats, and safety glasses, gloves, and masks when appropriate. In addition, special attention is paid to maintaining aseptic technique to minimize contamination and safety concerns. All chemicals used in the lab are stored, handled, and used as recommended by the manufacturer, and are disposed of in accordance with national, state, and local regulations and recommendations.


The organisms used in our project, Saccharomyces cerevisiae (BY4741) and Escherichia coli (JW2004-1 and NEB5α), are both labeled Risk Group 1 organisms by the World Health Organization in their Laboratory Biosafety Manual. This means that these organisms are low risk, and do not cause disease in healthy adult humans. All microbial strains used in the lab are either rendered antibiotic resistant by transformation, or they harbor nutritional auxotrophies or other mutations that mitigate the risk of growth outside of the laboratory or the ability of the organism to cause disease in healthy humans or animals.Furthermore, none of our recombinant constructs produce any known contagion or toxin.