Team:Goettingen/proLP

From 2014.igem.org

(Difference between revisions)

Latest revision as of 21:17, 17 October 2014

Overview

PCR Methods

Plasmid Construction

Plasmid Transformation

Colony Scanning

Protein Assessment

In vivo tests

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 	    <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR">PCR Methods</a></h4>
 	    <ul>
-	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR">Fusion PCR</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>PfuS</i> PCR</a></li>
-       	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Gra_PCR">Gradient PCR</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Fusi_PCR">Fusion PCR</a></li>
+            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#E_col"><i>E.coli</i> colony PCR</a></li>
+            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Y_col">Yeast colony PCR</a></li>
 	    </ul>
-	    <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA">DNA Assessment</a></h4>
-	    <ul>
-            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Gel_Ext">Gel Extraction</a></li>
-	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Gel_Ele">DNA Gel Electrophoresis</a></li>
-            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Seq">Pretreatment of DNA for Sequencing</a></li>
-            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#Flu">Fluorescence Assay</a></li>
-            </ul>
              <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4>
              <ul>
+            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#rest_DNA">Restriction of DNA</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#liga_DNA">Ligation of DNA fragments</a></li>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li>
+            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#seam_clone">SEAMLESS Cloning</a></li>
+            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LB">Peptide Library construction</a></li>
              </ul>
              <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran">Plasmid Transformation</a></h4>
              <ul>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_comp"><i>E.coli</i> competent cells</a></li>
+            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_plas">Plasmid isolation (<i>E.coli</i>)</a></li>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#E_tran"><i>E.coil</i> transformation</a></li>
-             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation</a></li>
+             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_iso">Plasmid isolation (Yeast)</a></li>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Tran#Y_tran">Yeast transformation</a></li>
              </ul>
              <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony">Colony Scanning</a></h4>
              <ul>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li>
-            <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y_col">Yeast colony PCR</a></li>
              <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li>
-             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_Scr">Yeast-Two-Hybrid Screening</a></li>
+             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Yeast-Two-Hybrid Assay with Robot</a></li>
-             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_bw">Y2H blue/white selection</a></li>
+             <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_screen">Yeast-Two-Hybrid Screening</a></li>
              </ul>
+	        <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li></h4>
+		<ul>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#SDS">SDS PAGE</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Coo">Coomassie Staining</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#P_Exp">Protein Expression</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Puri">Protein Purification</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Dialysis">Protein Dialysis </a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li>
+		</ul>
+	        <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo"><i>In vivo</i> tests</a></li></h4>
+		<ul>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Prod">Production of spore suspension</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Culti_A">Cell cultivation</a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#immu">Immunofluorescence Staining </a></li>
+	    <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Calc">Calcofluor staining</a></li>
+		</ul>
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