Team:Brasil-SP/Notebook/Protocols

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<b>The content of this page is merely provisional!</b>
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<p>Well... the page title describe the page by itself!</p>
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<h1 align=center>Experimental Methodology</h1>
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<p>For the assemblies constructuion we addopted the following method:</p>
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<p>We used the pSB1C3 vector from the Biobrick <a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a>. Using this biobrick we added a red/white selection, where the red colonies are those which do not have the assembly, and the white ones are the correct ones.</p>
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<div align=center><img src="https://static.igem.org/mediawiki/2014/2/25/Red_and_WHite_plate_.jpg" width="700" height="265"/></div>
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<a href="https://2014.igem.org/Team:Brasil-SP"style="color:#000000">Home </a> </td>
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<br>
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<a href="https://2014.igem.org/Team:Brasil-SP/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Brasil-SP"style="color:#000000"> Official Team Profile </a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Parts"style="color:#000000"> Parts</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Notebook"style="color:#000000"> Notebook</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Safety"style=" color:#000000"> Safety </a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Attributions"style="color:#000000"> Attributions </a></td>
 
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
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    <li><a href="https://static.igem.org/mediawiki/2014/1/1c/Electrophoresis_and_DNA_Gel_Purification.pdf">Agarose Gel Electrophoresis and DNA Gel Purification</a></li>
 
     <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf">PCR</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf">PCR</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf">Transformation in E. coli DH5-alpha</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf">Agarose Gel Electrophoresis and DNA Gel Purification</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf">PCR Product cloning</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/2/23/PCR_Product_Clonig_2.pdf">PCR Product cloning</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf">Competent <i>E. coli</i> Cells using Calcium Chloride</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf">Transformation in <i>E. coli</i> DH5-alpha</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/a/ac/Plasmid_preparation_by_alkaline_lysis_and_SDS.pdf">Plasmid preparation by alkaline lysis and SDS</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf">Cohesive-end assembly cloning</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf">Restriction Analysis and Digestion in large scale</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/6/66/Bacillus_Competent_cells.pdf">Competent <i>Bacillus subtilis</i> cells</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/a/a6/Transformation.pdf">Transformation of <i>Bacillus subtilis</i></a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/3/34/DSM_Medium_Sporulation.pdf">DSM medium for sporulation</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/0/00/Medium_LB.pdf">Luria-Bertani (LB) medium</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/0/0e/Neubauer_chamber_2.pdf">Cell counting with Neubauer Chamber</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/a/a2/Germination_Sporulation.pdf">Germination of <i>Bacillus subtilis</i> spores</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf">QuickChange PCR</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf">PCR for Synthesis</a></li>
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<h2 align="left"><b>Characterization Protocols</b></h2>
<h2 align="left"><b>Characterization Protocols</b></h2>
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    <li><a href="https://static.igem.org/mediawiki/2014/0/00/Cytometry_protocol_characterization.pdf">Cytometry Protocol</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/0/02/Fluorometry_Protocol_Characterization.pdf">Fluorometry Protocol</a></li>
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<p><h2>Measurement Interlab Study</h2></p>
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<p> As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
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<p align="center"> <br> Biobrick device 1: BBa_I20260, an existing device </p>
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<a href="https://static.igem.org/mediawiki/2014/c/c1/Assembly_Biobrick_Device_1_Measurement_Brasilsp.pdf">Assembly Protocol Biobrick Device 1</a>
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<p align="center"> <br> Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team  </p>
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<p align="center"> <br> Biobrick devide 3: BBa_J23115 + BBa_E0240, a new device built by each team.</p>
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<a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
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