Team:TU Delft-Leiden/WetLab/landmine

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        Module Landmine Detection
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<h2> Landmine Module</h2>
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<h3> Background Information</h3>
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        Information with respect to literature consulted regarding the Modules is referred to under Context. Also, each of the three complementary Modules is equipped with an Integration of Departments, in which it is described how the Departments Modeling, Experimental Work and Microfluidics interact. Furthermore, each Module contains information on Cloning and results are presented under Characterization.
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        <ul>Module Landmine Detection
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            <li><a href="/Team:TU_Delft-Leiden/WetLab/landmine/theory">Context</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/landmine/integration">Integration of Departments</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/WetLab/landmine/cloning">Cloning</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/WetLab/landmine/characterisation">Characterization</a></li>
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On the literature [1] a very interesting type of biosensor was found: two natural promoters of Escherichia coli (ybiJ and yqjF) were found to be activated in the presence of some aromatic N-based compounds such as 2,4,6-Trinitrotoluene (2,4,6-TNT), 2,4-Dinitrotoluene (2,4-DNT) and 1,3-Dinitrobenzene (1,3-DNB). Land mines are mainly composed of 2,4,6-TNT, but many times impurities of 2,4-DNT and 1,3-DNB are also present. These last two compounds are more volatile than 2,4,6-TNT and, therefore, tool they can more easily leak out of the land mine. As a consequence, Belkin and co-workers envisioned in the two aforementioned promoters (ybiJ and yqjF) a high potential to develop a biosensor for land mine detection.
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Keen to see our <b>conclusions</b> for this module?  See the list below!
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This was a very attractive case of study to implement the Plug-and-Play biosensor based on electrical current developed by our team.
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<li>The two best BioBricks for Landmine detection are LD5 (p[J] incl. N-enzymes) and LD4 (p[F] incl. N-enzymes). The N-genes contribute to sensitivity of the promoters.</li>
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<li> The LD5 construct seems to be the most suitable construct for Landmine Detection. </li>
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<h3> References </h3>
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<li> Evidence suggests that the ybiJ and yqjF promoters are activated by 2,4-DNT, but it cannot be totally discarded the possibility that acetonitrile is the trigger of the response of these promoters. </li></ul></p>
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<p>[1] S. Yagur-Kroll, S. Belkin <i>et al.</i>, “<i>Escherichia Coli</i> bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014. </p>
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<p>Want to know how we came to these conclusions? Go to our <a href= https://2014.igem.org/Team:TU_Delft-Leiden/WetLab/landmine/characterisation><b>Characterization</b></a> page!
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        Interested in one of our other Modules? Navigate to the <a href=" https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/EET"> <b> Module Electron Transport </b> </a> where you can find everything regarding the implementation of the MtrCAB conduit. Interested in living materials? Go to our  <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli"> <b> Module Conductive Curli </b> </a> project and find out how you can combine the benefits of both living- and non-living materials.
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        <img title="Deminer" src="https://static.igem.org/mediawiki/2014/5/5b/Deminer.jpg" style="display:block;margin-left:auto;margin-right:auto;height:50%;width:50%;" />
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Latest revision as of 20:54, 17 October 2014

Module Landmine Detection

Information with respect to literature consulted regarding the Modules is referred to under Context. Also, each of the three complementary Modules is equipped with an Integration of Departments, in which it is described how the Departments Modeling, Experimental Work and Microfluidics interact. Furthermore, each Module contains information on Cloning and results are presented under Characterization.

Keen to see our conclusions for this module? See the list below!

  • The two best BioBricks for Landmine detection are LD5 (p[J] incl. N-enzymes) and LD4 (p[F] incl. N-enzymes). The N-genes contribute to sensitivity of the promoters.
  • The LD5 construct seems to be the most suitable construct for Landmine Detection.
  • Evidence suggests that the ybiJ and yqjF promoters are activated by 2,4-DNT, but it cannot be totally discarded the possibility that acetonitrile is the trigger of the response of these promoters.

Want to know how we came to these conclusions? Go to our Characterization page!


Interested in one of our other Modules? Navigate to the Module Electron Transport where you can find everything regarding the implementation of the MtrCAB conduit. Interested in living materials? Go to our Module Conductive Curli project and find out how you can combine the benefits of both living- and non-living materials.

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