Team:SZU-China/notebook

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     <div class="col-lg-offset-3" > <a href="https://2014.igem.org/Team:SZU-China"><img src="https://static.igem.org/mediawiki/2014/d/df/SzuLogo2.0.png"><img src="https://static.igem.org/mediawiki/2014/2/2a/SzuLogoName.png"></a>
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           <li id="menu-item-215" class="menu-item menu-item-type-custom menu-item-object-custom current-menu-item current_page_item menu-item-home menu-item-215"><a title="Home" href="https://2014.igem.org/Team:SZU-China"><i class="fa fa-home"></i> Home</a></li>
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           <li id="menu-item-215" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-home menu-item-215"><a title="Home" href="https://2014.igem.org/Team:SZU-China"><i class="fa fa-home"></i> Home</a></li>
           <li id="menu-item-895" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-895"><a title="Project" ><i class=" fa fa-edit"></i> Project</a>
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               <li id="menu-item-896" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-896"><a href="https://2014.igem.org/Team:SZU-China/Project/background">background</a></li>
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               <li id="menu-item-897" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-897"><a href="https://2014.igem.org/Team:SZU-China/Project/overview">overview</a></li>
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               <li id="menu-item-897" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-897"><a href="https://2014.igem.org/Team:SZU-China/Project/overview">Overview</a></li>
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               <li id="menu-item-898" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-898"><a href="https://2014.igem.org/Team:SZU-China/Project/design">design</a></li>
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               <li id="menu-item-896" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-896"><a href="https://2014.igem.org/Team:SZU-China/Project/AlkalineCellulase">Alkaline Cellulase</a></li>
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               <li id="menu-item-899" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-899"><a href="https://2014.igem.org/Team:SZU-China/Project/result">result</a></li>
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            <li id="menu-item-898" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-898"><a href="https://2014.igem.org/Team:SZU-China/Project/Mdfa">Mdfa Antiporter</a></li>
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               <li id="menu-item-899" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-899"><a href="https://2014.igem.org/Team:SZU-China/Project/Kil">Kil Protein</a></li>
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              <li id="menu-item-900" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-900"><a href="https://2014.igem.org/Team:SZU-China/Project/ccdB">ccdB Suicide System</a></li>
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                <li id="menu-item-901" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-901"><a href="https://2014.igem.org/Team:SZU-China/Project/Collaboration">Collaboration</a></li>
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           <li id="menu-item-938" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-938"><a  title="Team" ><i class="fa  fa-group"></i> Team</a>
           <li id="menu-item-938" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-938"><a  title="Team" ><i class="fa  fa-group"></i> Team</a>
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               <li id="menu-item-997" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-997"><a href="https://2014.igem.org/Team:SZU-China/Team/member">member</a></li>
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               <li id="menu-item-997" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-997"><a href="https://2014.igem.org/Team:SZU-China/Team/member">Member</a></li>
               <li id="menu-item-996" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-996"><a href="https://2014.igem.org/Team:SZU-China/Team/Attribution">Attribution</a></li>
               <li id="menu-item-996" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-996"><a href="https://2014.igem.org/Team:SZU-China/Team/Attribution">Attribution</a></li>
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              <li id="menu-item-939" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-939"><a href="https://igem.org/Team.cgi?year=2014">official team profile</a></li>
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              <li id="menu-item-940" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-940"><a href="https://2014.igem.org/Team:SZU-China/Team/Acknowledgment">Acknowledgment</a></li>
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              <li id="menu-item-939" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-939"><a href="https://igem.org/Team.cgi?year=2014">Official team profile</a></li>
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           <li id="menu-item-676" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-676"><a><i class="fa fa-globe"></i> Human Practice</a>
           <li id="menu-item-676" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-676"><a><i class="fa fa-globe"></i> Human Practice</a>
             <ul class="dropdown-menu">
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            <li id="menu-item-676" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-676"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Overview">Overview</a></li>
               <li id="menu-item-677" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-677"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/CulturalFestival">Cultural Festival</a></li>
               <li id="menu-item-677" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-677"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/CulturalFestival">Cultural Festival</a></li>
               <li id="menu-item-678" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-678"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Communication">Communication</a></li>
               <li id="menu-item-678" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-678"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Communication">Communication</a></li>
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               <li id="menu-item-680" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-680"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/PaperMill">PaperMill</a></li>
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               <li id="menu-item-680" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-680"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/PaperMill">Paper Mill</a></li>
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              <li id="menu-item-680" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-680"><a target="_blank" href="https://2014.igem.org/Team:SZU-China/HumanPractice/Survey">Survey</a></li>
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             </ul>
             </ul>
           </li>
           </li>
           <li id="menu-item-607" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-607"><a title="Judging"><i class="fa fa-check"></i> Judging</a>
           <li id="menu-item-607" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-607"><a title="Judging"><i class="fa fa-check"></i> Judging</a>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/achievement">achievement</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/achievement">Achievement</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/biobrick">biobrick</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/judging/biobrick">Biobrick</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/Safety">safety</a></li>
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               <li class="menu-item menu-item-type-custom menu-item-object-custom"> <a target="_blank" href="https://2014.igem.org/Team:SZU-China/Safety">Safety</a></li>
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             </ul>
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           <li id="menu-item-1070" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-1070"><a href="https://2014.igem.org/Team:SZU-China/notebook"><i class="fa fa-book"></i>NoteBook</a></li>
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           <li id="menu-item-1070" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-1070 current-menu-item current_page_item"><a href="https://2014.igem.org/Team:SZU-China/notebook"><i class="fa fa-book"></i>NoteBook</a></li>
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<div class="related-title"><h1><span class="label label-success">Our Notebook</span></h1> </div>
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        <div class="slide-text hidden-xs"> Cultural Festival </div>
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  <div class="panel-heading">Mdfa team</div>
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  <li> 01/05/2014-15/06/2014:Research about the previous work of iGEM.</li>
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<li>15/06/2014: Project idea discussing and determination， group issue asssigned.</li>
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<li>01/07/2014-22/07/2014: Experiments process design, Meet up with SUSTC-Shenzhen.</li>
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<li>23/07/2014-01/08/2014: Design the primers needed to amplification the Mdfa</li>
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        <div class="slide-text hidden-xs">Cultural Festival</div>
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<li>04/08/2014-24/08/2014: Constructs the basic parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350005" class="alert-info">BBa_K1350005</a>.Visit the paper mill.</li>
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<li>25/08/2014-07/09/2014: Constructs the basic parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350006" class="alert-info">BBa_K1350006</a>.</li>
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<li>08/09/2014-25/09/2014: Test function of BBa_K1350006 and then data analysis</li>
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<li>01/10/2014-06/10/2014: Constructs the basic parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350010" class="alert-info">BBa_K1350010</a>.</li>
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<li>06/10/2014-15/10/2014:. Results drawing and made the wiki.</li>
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    <h3 class="panel-title">Kil team</h3>
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    <ul>
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    <li>01/05/2014-15/06/2014: Study previous team wikis.</li>
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<li>15/06/2014: Project idea discussing and determination， group issue asssigned.</li>
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<li>01/07/2014-22/07/2014: Experiments process design. Meet up with SUSTC-Shenzhen.</li>
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<li>01/08/2014-22/08/2014:By gene synthesis to get Kil gene. Visit the paper mill.</li>
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<li>23/08/2014-01/09/2014: Constructs the basic parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350001" class="alert-info">BBa_K1350001</a>.</li>
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<li>02/09/2014-14/09/2014: Constructs the basic parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350002" class="alert-info">BBa_K1350002</a>.</li>
 +
<li>15/09/2014-30/09/2014: Test function of BBa_K1350002 and then data analysis</li>
 +
<li>1/10/2014/2014-15/10/2014: Results drawing and made the wiki</li>
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  <div class="category-cloud"><a href="http://blog.zanwp.com/archives/category/zanblog"  rel="category tag">What we do</a> <a href="http://blog.zanwp.com/archives/category/slide" title="查看幻灯片中的全部文章" rel="category tag">幻灯片</a></div>
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  <h3> Super Dream Factory of Cellulase </h3>
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     <h3 class="panel-title">Cellulase team</h3>
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      <figure class="thumbnail zan-thumb"><a href="http://blog.zanwp.com/archives/1094.html"><img width="400" height="240" src="https://static.igem.org/mediawiki/2014/c/c2/SzuDesign1.png"  class="lazy attachment-medium wp-post-image" alt="banner_7" />
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    <div class="col-md-7 visible-lg bg-info 微软雅黑"> To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na+/K+ at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 10. Another highlight of our project is that we also added a ccdAB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety. <br>
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   <div class="pull-right post-info"> <span><i class="fa fa-comment"></i> From SZU-China</span> </div></div>
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    <li>01/05/2014-15/06/2014:Study the previous work of iGEM.</li>
 +
<li>15/06/2014: Project idea discussing and determination， group issue asssigned.</li>
 +
<li>01/07/2014-22/07/2014: Experiments process design. Meet up with SUSTC-Shenzhen.</li>
 +
<li>23/07/2014-01/08/2014: Design the primers needed to amplification the cellulase</li>
 +
<li>01/08/2014-15/08/2014: Test function of cellulase in BL21 and then data analysis</li>
 +
<li>19/08/2014-20/09/2014: Constructs the basic parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350003" class="alert-info">BBa_K1350003</a>.</li>
 +
<li>22/09/2014/2014-15/10/2014: Results drawing and made the wiki</li>
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    <h3 class="panel-title">ccdB team</h3>
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  </div>
 +
  <div class="panel-body">
 +
    <ul>
 +
  <li> 01/05/2014-15/06/2014:Research about the previous work of iGEM.</li>
 +
<li>15/06/2014: Project idea discussing and determination， group issue asssigned.</li>
 +
<li>01/07/2014-22/07/2014: Experiments process design,. Meet up with SUSTC-Shenzhen.</li>
 +
<li>01/08/2014-06/08/2014:Study how to use the 2014 DNA Distribution Kit.</li>
 +
<li>10/08/2014-31/08/2014: Constructs the basic parts:  <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1350003" class="alert-info">BBa_K1350000</a>.Visit the paper mill.</li>
 +
<li>01/09/2014-10/09/2014: Test the function of BBa_K1350000 and then data analysis.</li>
 +
<li>19/09/2014/2014-15/10/2014: Results drawing and made the wiki.</li>
 +
 
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Latest revision as of 02:40, 18 October 2014

SZU-China

Our Notebook

Mdfa team
  • 01/05/2014-15/06/2014:Research about the previous work of iGEM.
  • 15/06/2014: Project idea discussing and determination， group issue asssigned.
  • 01/07/2014-22/07/2014: Experiments process design, Meet up with SUSTC-Shenzhen.
  • 23/07/2014-01/08/2014: Design the primers needed to amplification the Mdfa
  • 04/08/2014-24/08/2014: Constructs the basic parts: BBa_K1350005.Visit the paper mill.
  • 25/08/2014-07/09/2014: Constructs the basic parts: BBa_K1350006.
  • 08/09/2014-25/09/2014: Test function of BBa_K1350006 and then data analysis
  • 01/10/2014-06/10/2014: Constructs the basic parts: BBa_K1350010.
  • 06/10/2014-15/10/2014:. Results drawing and made the wiki.

Kil team

  • 01/05/2014-15/06/2014: Study previous team wikis.
  • 15/06/2014: Project idea discussing and determination， group issue asssigned.
  • 01/07/2014-22/07/2014: Experiments process design. Meet up with SUSTC-Shenzhen.
  • 01/08/2014-22/08/2014:By gene synthesis to get Kil gene. Visit the paper mill.
  • 23/08/2014-01/09/2014: Constructs the basic parts: BBa_K1350001.
  • 02/09/2014-14/09/2014: Constructs the basic parts: BBa_K1350002.
  • 15/09/2014-30/09/2014: Test function of BBa_K1350002 and then data analysis
  • 1/10/2014/2014-15/10/2014: Results drawing and made the wiki

Cellulase team

  • 01/05/2014-15/06/2014:Study the previous work of iGEM.
  • 15/06/2014: Project idea discussing and determination， group issue asssigned.
  • 01/07/2014-22/07/2014: Experiments process design. Meet up with SUSTC-Shenzhen.
  • 23/07/2014-01/08/2014: Design the primers needed to amplification the cellulase
  • 01/08/2014-15/08/2014: Test function of cellulase in BL21 and then data analysis
  • 19/08/2014-20/09/2014: Constructs the basic parts: BBa_K1350003.
  • 22/09/2014/2014-15/10/2014: Results drawing and made the wiki

ccdB team

  • 01/05/2014-15/06/2014:Research about the previous work of iGEM.
  • 15/06/2014: Project idea discussing and determination， group issue asssigned.
  • 01/07/2014-22/07/2014: Experiments process design,. Meet up with SUSTC-Shenzhen.
  • 01/08/2014-06/08/2014:Study how to use the 2014 DNA Distribution Kit.
  • 10/08/2014-31/08/2014: Constructs the basic parts: BBa_K1350000.Visit the paper mill.
  • 01/09/2014-10/09/2014: Test the function of BBa_K1350000 and then data analysis.
  • 19/09/2014/2014-15/10/2014: Results drawing and made the wiki.
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