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Team:Brasil-SP/Notebook
From 2014.igem.org
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| - | < | + | <!--main content --> |
| - | + | <table width="70%" align="center"> | |
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| + | <h1 align=center>Main Assembly Map</h1> | ||
| - | </ | + | <p><div align="justify"> The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p> |
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| + | </style> | ||
| + | <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/8/87/Mapa.png');"> | ||
| + | <a class="map_link" id="map_link_1" title="" href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a> | ||
| + | <a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a> | ||
| + | <a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> | ||
| + | <a class="map_link" id="map_link_4" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a> | ||
| + | <a class="map_link" id="map_link_5" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a> | ||
| + | <a class="map_link" id="map_link_6" title="" href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> | ||
| + | <a class="map_link" id="map_link_7" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | ||
| + | <a class="map_link" id="map_link_8" title="" href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a> | ||
| + | <a class="map_link" id="map_link_9" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a> | ||
| + | <a class="map_link" id="map_link_10" title="" href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> | ||
| + | <a class="map_link" id="map_link_11" title="" href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> | ||
| + | </div> | ||
| - | < | + | <h1 align=center>Assemblies forms</h1> |
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| - | </ | + | |
| - | < | + | <p><strong>Assembly form template</strong></p> |
| - | < | + | <p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p> |
| - | </ | + | |
| + | <div align=center> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a> | ||
| + | </div> | ||
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| - | + | <h1 align=center><strong>Life Inside the LAB</strong></h1> | |
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| - | < | + | <thead><h2 align=center>July</h2> |
| - | + | </thead> | |
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| - | < | + | <tbody> |
| - | <table | + | <table> |
<tr> | <tr> | ||
| - | <th | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>30/06 |
<ul> | <ul> | ||
| - | <li>PCR lasR (BBa_C0079) | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>01/07 |
<ul> | <ul> | ||
| - | <li>Purification of lasR PCR product </li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>02/07 |
<ul> | <ul> | ||
| - | <li>Transformation of the Biobricks:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
| - | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
| - | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
<li>Inoculum of the Biobricks sent by iGEM HQ:</li> | <li>Inoculum of the Biobricks sent by iGEM HQ:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K316037</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
| - | <li>BBa_K316018</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
| - | <li>BBa_K316015</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>03/07 |
<ul> | <ul> | ||
| - | <li>Transformation of the Biobricks sent by the Imperial College Team:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li> |
| - | <li>BBa_K143031</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li> |
</ul> | </ul> | ||
| - | <li>Miniprep, Quantification of the plasmidial DNA samples and Restriction Analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>04/07</td> |
| - | < | + | <td>05/07</td> |
| - | < | + | <td>06/07 |
<ul> | <ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
| - | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
| - | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>07/07 |
<ul> | <ul> | ||
| - | <li>Miniprep, Quantification and Restriction Analysis: | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
| - | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
| - | <li>BBa_E0840</li> | + | <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>08/07</td> |
| - | < | + | <td>09/07 |
<ul> | <ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>10/07 |
<ul> | <ul> | ||
| - | <li>Miniprep, Quantification and Restriction Analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_B0015 (restriction analysis fail, repeat)</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li> |
</ul> | </ul> | ||
| - | <li>PCR qteE for amplification | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li> |
| - | <li>Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>11/07 |
<ul> | <ul> | ||
<li>Prepare 40% glycerol solution</li> | <li>Prepare 40% glycerol solution</li> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>RBS + lasR (BBa_C0079)</li> | + | <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li> |
</ul> | </ul> | ||
| - | <li>Purification:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> |
<ul> | <ul> | ||
<li>RBS + qteE</li> | <li>RBS + qteE</li> | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>12/07</td> |
| - | < | + | <td>13/07</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>14/07 |
<ul> | <ul> | ||
<li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
<li>Prepare X-gal</li> | <li>Prepare X-gal</li> | ||
<li>Prepare more LB medium (solid + liquid)</li> | <li>Prepare more LB medium (solid + liquid)</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>15/07 |
<ul> | <ul> | ||
<li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | ||
| Line 347: | Line 246: | ||
</ul> | </ul> | ||
<li>Replate RBS+lasR</li> | <li>Replate RBS+lasR</li> | ||
| - | <li>Ligation of PCR qteE in pGEM T-easy vector</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li> |
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li> |
</ul> | </ul> | ||
| - | <li>Restriction analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>16/07 |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Electrophoresis analysis:</li> | <li>Electrophoresis analysis:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>17/07 |
<ul> | <ul> | ||
| - | <li>Glycerol Stock and Miniprep:<li> | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
<li>RBS+lasR</li> | <li>RBS+lasR</li> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
| - | <li>Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
| - | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
</ul> | </ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
<ul> | <ul> | ||
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>18/07 |
<ul> | <ul> | ||
| - | <li>Glycerol Stock and Miniprep:</li> | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
| - | <li>Gel Purification:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
| - | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
| - | <li>Restriction analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>19/07</td> |
| - | < | + | <td>20/07</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>21/07 |
<ul> | <ul> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>22/07 |
<ul> | <ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
| - | <li>Miniprep, Restriction analysis and Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
| Line 444: | Line 343: | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
| - | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>23/07 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
| - | <li>Prepare more competent cells</li> | + | <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li> |
<li>Prepare LB medium (solid and liquid)</li> | <li>Prepare LB medium (solid and liquid)</li> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | <li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>24/07 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
| Line 470: | Line 369: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
| - | <li>Miniprep:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>25/07 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
| - | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>26/07</td> |
| - | < | + | <td>27/07</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>28/07 |
<ul> | <ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Restriction analysis (+NdeI enzyme)</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li> |
| - | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that | + | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 500: | Line 399: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>29/07 |
<ul> | <ul> | ||
<li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 510: | Line 409: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
| - | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>30/07 |
<ul> | <ul> | ||
<li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 521: | Line 420: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>31/07 |
<ul> | <ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 533: | Line 432: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
| - | <li>PCR BBa_K143055 for RBS removal</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li> |
<li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | ||
| - | <li>PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector</li> | + | <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| - | |||
| - | </ | + | </tbody> |
| - | < | + | <thead><h2 align=center>August</h2> |
| + | |||
| + | </thead> | ||
| + | |||
| + | </tbody> | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| - | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td>01/08 |
<ul> | <ul> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 576: | Line 481: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
| - | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>02/08</td> |
| - | < | + | <td>03/08</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>04/08 |
<ul> | <ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
| Line 591: | Line 496: | ||
<li>E. coli DH5-alpha</li> | <li>E. coli DH5-alpha</li> | ||
<li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | ||
| - | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
<ul> | <ul> | ||
| - | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
<li>Prepare more inoculum</li> | <li>Prepare more inoculum</li> | ||
| - | <li>Repeat Restriction analysis | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>05//08 |
<ul> | <ul> | ||
<li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | ||
| - | <li>Miniprep:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
| - | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
| - | <li>And once again! Restriction analysis (this BBa_K316016 is tough)</li> | + | <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>06/08</td> |
| - | < | + | <td>07/08 |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>08/08</td> |
| - | < | + | <td>09/08</td> |
| - | < | + | <td>10/08</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>11/08 |
<ul> | <ul> | ||
| - | <li>Prepare Plac BBa_K143055 for sequencing</li> | + | <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li> |
<li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>12/08 |
<ul> | <ul> | ||
| - | <li>Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
| Line 650: | Line 555: | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>13/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>14/08 |
<ul> | <ul> | ||
| - | <li>Gel Purification and Ligation in pSB1C3 for BBiobrick standard:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
| Line 669: | Line 574: | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| - | <li>Gel Purification</li> | + | <ul> |
| - | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | |
| + | <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li> | ||
| + | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>15/08</td> |
| - | < | + | <td>16/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>17/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>18/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
<li>Prepare LB medium (liquid and solid)</li> | <li>Prepare LB medium (liquid and solid)</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>19/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>20/08 |
<ul> | <ul> | ||
<li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| Line 720: | Line 627: | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>21/08 |
<ul> | <ul> | ||
<li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| Line 727: | Line 634: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li> |
| - | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>22/08 |
<ul> | <ul> | ||
<li>Prepare LB medium (liquid and solid)</li> | <li>Prepare LB medium (liquid and solid)</li> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Repeat restriction analysis adding the enzyme NdeI | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>23/08</td> |
| - | < | + | <td>24/08</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>25/08 |
<ul> | <ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
| - | <li>PCR BBa_K143055 with higher melting temperature | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li> |
<ul> | <ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>26/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| Line 762: | Line 669: | ||
<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>27/08 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| Line 774: | Line 681: | ||
<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | ||
<ul> | <ul> | ||
| - | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>28/08</td> |
| - | < | + | <td>29/08</td> |
| - | < | + | <td>30/08</td> |
| - | < | + | <td>31/08</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| - | < | + | <thead><h2 align=center>September</h2> |
| - | </ | + | </thead> |
| - | < | + | <tbody> |
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| - | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>01/09 |
| - | + | <ul> | |
| - | < | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV. |
| - | < | + | </ul> |
| - | < | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, |
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>02/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>03/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>04/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>05/09</td> | ||
| + | <td>06/09</td> | ||
| + | <td>07/09</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>08/09 |
| - | + | <ul> | |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> |
| - | < | + | </ul> |
| - | < | + | </td> |
| - | < | + | <td>09/09</td> |
| + | <td>10/09 | ||
| + | <ul> | ||
| + | <li>The QuickChange protocol didn't work well. So try again with more attention!</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>11/09 | ||
| + | <ul> | ||
| + | <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>12/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li> | ||
| + | </ul> | ||
| + | <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>13/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | <li>Prepare LB medium (solid and liquid)</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>14/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>15/09 |
| - | + | <ul> | |
| - | < | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
| - | < | + | </ul> |
| - | < | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, |
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>16/09 | ||
| + | <ul> | ||
| + | <li>Prepare 40% glycerol solution</li> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>17/09 | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | |||
| + | <ul> | ||
| + | <li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li> | ||
| + | </ul> | ||
| + | |||
| + | </td> | ||
| + | <td>18/09 | ||
| + | <ul> | ||
| + | <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>19/09</td> | ||
| + | <td>20/09</td> | ||
| + | <td>21/09</td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>22/09 |
| - | + | <ul> | |
| - | < | + | <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
| - | < | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
| - | < | + | </ul> |
| + | </ul> | ||
| + | |||
| + | </td> | ||
| + | <td>23/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>24/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>25/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>26/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>27/09</td> | ||
| + | <td>28/09</td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>29/09 |
| - | + | <ul> | |
| - | < | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>30/09 | ||
| + | <ul> | ||
| + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
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| - | < | + | |
| + | <thead><h2 align=center>October</h2> | ||
| + | |||
| + | </thead> | ||
| + | |||
| + | <tbody> | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| - | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td>01/10 |
| - | < | + | <ul> |
| - | < | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> |
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>02/10</td> | ||
| + | <td>03/10</td> | ||
| + | <td>04/10</td> | ||
| + | <td>05/10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>06/10 |
| - | < | + | <ul> |
| - | < | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>07/10 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>08/10 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | </td> | ||
| + | <td>09/10 | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>10/10 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>11/10</td> | ||
| + | <td>12/10</td> | ||
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Latest revision as of 20:32, 17 October 2014
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