Team:ITESM-CEM/Parts
From 2014.igem.org
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<td colspan="3" rowspan="3" align="left" valign="top"><ul> | <td colspan="3" rowspan="3" align="left" valign="top"><ul> | ||
- | <sub> | + | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub> |
- | + | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List">List of our parts</a></sub> | |
+ | </ul></td> | ||
<td><img src="images/spacer.gif" width="1" height="1" alt=""></td> | <td><img src="images/spacer.gif" width="1" height="1" alt=""></td> | ||
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<table width="100%" border="0" id="ContenidoSecciones"> | <table width="100%" border="0" id="ContenidoSecciones"> | ||
<div style="background-color: #f3f3e2; style="width:95%"> | <div style="background-color: #f3f3e2; style="width:95%"> | ||
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<!--INICIO CONTENIDO--> | <!--INICIO CONTENIDO--> | ||
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- | <h2> | + | <h2>Our Parts</h2> |
- | < | + | <iframe align="right" width="660" height="515" src="//www.youtube.com/embed/1mFwPDbb7UY" frameborder="0" allowfullscreen></iframe> |
- | </p> | + | |
+ | <p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis. | ||
+ | </p><br> | ||
+ | |||
+ | <h2>Cholesterol Oxidase</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. | ||
+ | </p> <br> | ||
+ | |||
+ | <h2>Oxoacyl Reductase</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in <u>Rhodococcus jostii </u>. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. | ||
+ | </p> <br> | ||
+ | |||
+ | <h2>7-dehydratase</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in <u>Rhodococcus jostii</u> . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on <u>E.coli</u>, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. | ||
+ | </p> <br> | ||
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+ | <h2>Neomycin Resistance</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A. | ||
+ | </p><br> | ||
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+ | <h2>BGHPA</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells. | ||
+ | </p><br> | ||
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+ | <h2>PCMV</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression. | ||
+ | </p><br><br><br> | ||
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+ | <br> | ||
<!--FIN CONTENIDO--> | <!--FIN CONTENIDO--> |
Latest revision as of 02:12, 18 October 2014
ITESM-CEM | Enzy7-K me |
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