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Team:Brasil-SP/Notebook
From 2014.igem.org
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| - | < | + | <!--main content --> |
| - | + | <table width="70%" align="center"> | |
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| + | <h1 align=center>Main Assembly Map</h1> | ||
| - | </ | + | <p><div align="justify"> The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p> |
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| + | </style> | ||
| + | <div class="map_image" style="background-image: url('https://static.igem.org/mediawiki/2014/8/87/Mapa.png');"> | ||
| + | <a class="map_link" id="map_link_1" title="" href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a> | ||
| + | <a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a> | ||
| + | <a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> | ||
| + | <a class="map_link" id="map_link_4" title="" href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a> | ||
| + | <a class="map_link" id="map_link_5" title="" href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a> | ||
| + | <a class="map_link" id="map_link_6" title="" href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> | ||
| + | <a class="map_link" id="map_link_7" title="" href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | ||
| + | <a class="map_link" id="map_link_8" title="" href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a> | ||
| + | <a class="map_link" id="map_link_9" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a> | ||
| + | <a class="map_link" id="map_link_10" title="" href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> | ||
| + | <a class="map_link" id="map_link_11" title="" href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> | ||
| + | </div> | ||
| - | < | + | <h1 align=center>Assemblies forms</h1> |
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| - | </ | + | |
| - | < | + | <p><strong>Assembly form template</strong></p> |
| - | < | + | <p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p> |
| - | </ | + | |
| + | <div align=center> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a> | ||
| + | </div> | ||
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| - | + | <h1 align=center><strong>Life Inside the LAB</strong></h1> | |
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| - | < | + | <thead><h2 align=center>July</h2> |
| - | + | </thead> | |
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| - | < | + | <tbody> |
| - | <table | + | <table> |
<tr> | <tr> | ||
| - | <th | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>30/06 |
<ul> | <ul> | ||
| - | <li>PCR lasR (BBa_C0079) | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>01/07 |
<ul> | <ul> | ||
| - | <li>Purification of lasR PCR product </li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>02/07 |
<ul> | <ul> | ||
| - | <li>Transformation of the Biobricks:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
| - | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
| - | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
<li>Inoculum of the Biobricks sent by iGEM HQ:</li> | <li>Inoculum of the Biobricks sent by iGEM HQ:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K316037</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
| - | <li>BBa_K316018</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
| - | <li>BBa_K316015</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>03/07 |
<ul> | <ul> | ||
| - | <li>Transformation of the Biobricks sent by the Imperial College Team:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li> |
| - | <li>BBa_K143031</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li> |
</ul> | </ul> | ||
| - | <li>Miniprep, Quantification of the plasmidial DNA samples and Restriction Analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> |
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li> |
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>04/07</td> |
| - | < | + | <td>05/07</td> |
| - | < | + | <td>06/07 |
<ul> | <ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
| - | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
| - | <li>BBa_E0840</li> | + | <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>07/07 |
<ul> | <ul> | ||
| - | <li>Miniprep, Quantification and Restriction Analysis: | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li> |
| - | <li>BBa_K081001</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li> |
| - | <li>BBa_E0840</li> | + | <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li> |
</ul> | </ul> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>08/07</td> |
| - | < | + | <td>09/07 |
<ul> | <ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>10/07 |
<ul> | <ul> | ||
| - | <li>Miniprep, Quantification and Restriction Analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_B0015 (restriction analysis fail, repeat)</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li> |
</ul> | </ul> | ||
| - | <li>PCR qteE for amplification | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li> |
| - | <li>Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>11/07 |
<ul> | <ul> | ||
<li>Prepare 40% glycerol solution</li> | <li>Prepare 40% glycerol solution</li> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>RBS + lasR (BBa_C0079)</li> | + | <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li> |
</ul> | </ul> | ||
| - | <li>Purification:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> |
<ul> | <ul> | ||
<li>RBS + qteE</li> | <li>RBS + qteE</li> | ||
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>12/07</td> |
| - | < | + | <td>13/07</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>14/07 |
<ul> | <ul> | ||
<li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
<li>Prepare X-gal</li> | <li>Prepare X-gal</li> | ||
<li>Prepare more LB medium (solid + liquid)</li> | <li>Prepare more LB medium (solid + liquid)</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>15/07 |
<ul> | <ul> | ||
<li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | <li>Assembly:<a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a></li> | ||
| Line 347: | Line 246: | ||
</ul> | </ul> | ||
<li>Replate RBS+lasR</li> | <li>Replate RBS+lasR</li> | ||
| - | <li>Ligation of PCR qteE in pGEM T-easy vector</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li> |
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li> |
</ul> | </ul> | ||
| - | <li>Restriction analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>16/07 |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Electrophoresis analysis:</li> | <li>Electrophoresis analysis:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>17/07 |
<ul> | <ul> | ||
| - | <li>Glycerol Stock and Miniprep:<li> | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
<li>RBS+lasR</li> | <li>RBS+lasR</li> | ||
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
| - | <li>Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
| - | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li> |
</ul> | </ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
<ul> | <ul> | ||
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>18/07 |
<ul> | <ul> | ||
| - | <li>Glycerol Stock and Miniprep:</li> | + | <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
| - | <li>BBa_B0015</li> | + | <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li> |
</ul> | </ul> | ||
| - | <li>Gel Purification:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_J04450 (EP)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li> |
| - | <li>BBa_J04450 (XS)</li> | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li> |
</ul> | </ul> | ||
| - | <li>Restriction analysis:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K823003</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>19/07</td> |
| - | < | + | <td>20/07</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>21/07 |
<ul> | <ul> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>22/07 |
<ul> | <ul> | ||
<li>Inoculum:</li> | <li>Inoculum:</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
| - | <li>Miniprep, Restriction analysis and Digestion:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
<ul> | <ul> | ||
<li>RBS+qteE</li> | <li>RBS+qteE</li> | ||
| Line 444: | Line 343: | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
| - | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>23/07 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
| - | <li>Prepare more competent cells</li> | + | <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li> |
<li>Prepare LB medium (solid and liquid)</li> | <li>Prepare LB medium (solid and liquid)</li> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
<li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | <li>Prepare RBS+lasR and RBS+qteE for sequencing</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>24/07 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
| Line 470: | Line 369: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
| - | <li>Miniprep:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K316016</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>25/07 |
<ul> | <ul> | ||
<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li> | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
| - | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>26/07</td> |
| - | < | + | <td>27/07</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>28/07 |
<ul> | <ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Restriction analysis (+NdeI enzyme)</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li> |
| - | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that | + | <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 500: | Line 399: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Digestion EXSP</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>29/07 |
<ul> | <ul> | ||
<li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 510: | Line 409: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Gel Purification</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
| - | <li>Ligation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>30/07 |
<ul> | <ul> | ||
<li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 521: | Line 420: | ||
<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>31/07 |
<ul> | <ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 533: | Line 432: | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
</ul> | </ul> | ||
| - | <li>PCR BBa_K143055 for RBS removal</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li> |
<li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) </li> | ||
| - | <li>PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector</li> | + | <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li> |
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| - | |||
| - | </ | + | </tbody> |
| - | < | + | <thead><h2 align=center>August</h2> |
| + | |||
| + | </thead> | ||
| + | |||
| + | </tbody> | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| - | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td>01/08 |
<ul> | <ul> | ||
| - | <li>Transformation:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_K143055</li> | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>, | ||
| Line 576: | Line 481: | ||
<ul> | <ul> | ||
<li>Glycerol Stock</li> | <li>Glycerol Stock</li> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
| - | <li>Restriction analysis</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>02/08</td> |
| - | < | + | <td>03/08</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>04/08 |
<ul> | <ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
| Line 591: | Line 496: | ||
<li>E. coli DH5-alpha</li> | <li>E. coli DH5-alpha</li> | ||
<li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li> | ||
| - | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
| - | <li>Transformation</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> |
<ul> | <ul> | ||
| - | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
<li>Prepare more inoculum</li> | <li>Prepare more inoculum</li> | ||
| - | <li>Repeat Restriction analysis | + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>05//08 |
<ul> | <ul> | ||
<li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li> | ||
| - | <li>Miniprep:</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li> |
<ul> | <ul> | ||
| - | <li>Plac BBa_K143055</li> | + | <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
</ul> | </ul> | ||
<li>Inoculum</li> | <li>Inoculum</li> | ||
<ul> | <ul> | ||
| - | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li> | ||
<ul> | <ul> | ||
| - | <li>Miniprep</li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> |
| - | <li>And once again! Restriction analysis (this | + | <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>06/08</td> |
| - | < | + | <td>07/08 |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li> |
<ul> | <ul> | ||
| - | <li>BBa_P0312</li> | + | <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li> |
</ul> | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>08/08</td> |
| - | < | + | <td>09/08</td> |
| - | < | + | <td>10/08</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>11/08 |
<ul> | <ul> | ||
| - | <li>Prepare Plac BBa_K143055 for sequencing</li> | + | <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li> |
<li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | < | + | <td>12/08 |
<ul> | <ul> | ||
| - | <li> | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li> |
| + | <ul> | ||
| + | <li>RBS+qteE</li> | ||
| + | <li>RBS+lasR</li> | ||
| + | <li>OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>13/08 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>14/08 | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li> | ||
| + | <ul> | ||
| + | <li>RBS+qteE</li> | ||
| + | <li>RBS+lasR</li> | ||
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>15/08</td> | ||
| + | <td>16/08 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>17/08 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | </ul> | ||
</ul> | </ul> | ||
| - | </ | + | </td> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>18/08 |
| - | < | + | <ul> |
| - | < | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> |
| - | < | + | </ul> |
| + | <li>Prepare LB medium (liquid and solid)</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>19/08 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>20/08 | ||
| + | <ul> | ||
| + | <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>21/08 | ||
| + | <ul> | ||
| + | <li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>22/08 | ||
| + | <ul> | ||
| + | <li>Prepare LB medium (liquid and solid)</li> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li> | ||
| + | <ul> | ||
| + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>23/08</td> | ||
| + | <td>24/08</td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>25/08 |
| - | < | + | <ul> |
| - | < | + | <li>Inoculum</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li> |
| - | < | + | <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li> |
| - | < | + | </ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li> | ||
| + | <ul> | ||
| + | </td> | ||
| + | <td>26/08 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>27/08 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>28/08</td> | ||
| + | <td>29/08</td> | ||
| + | <td>30/08</td> | ||
| + | <td>31/08</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| - | < | + | <thead><h2 align=center>September</h2> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | < | + | </thead> |
| + | |||
| + | <tbody> | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| - | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>01/09 |
| - | + | <ul> | |
| - | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a> | |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV. |
| - | < | + | </ul> |
| - | < | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, |
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>02/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>03/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>04/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>05/09</td> | ||
| + | <td>06/09</td> | ||
| + | <td>07/09</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>08/09 |
| - | + | <ul> | |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> |
| - | < | + | </ul> |
| - | < | + | </td> |
| - | < | + | <td>09/09</td> |
| + | <td>10/09 | ||
| + | <ul> | ||
| + | <li>The QuickChange protocol didn't work well. So try again with more attention!</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>11/09 | ||
| + | <ul> | ||
| + | <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>12/09 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li> | ||
| + | </ul> | ||
| + | <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>13/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | <li>Prepare LB medium (solid and liquid)</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>14/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>15/09 |
| - | + | <ul> | |
| - | < | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
| - | < | + | </ul> |
| - | < | + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, |
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>16/09 | ||
| + | <ul> | ||
| + | <li>Prepare 40% glycerol solution</li> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>17/09 | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | |||
| + | <ul> | ||
| + | <li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li> | ||
| + | </ul> | ||
| + | |||
| + | </td> | ||
| + | <td>18/09 | ||
| + | <ul> | ||
| + | <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>19/09</td> | ||
| + | <td>20/09</td> | ||
| + | <td>21/09</td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>22/09 |
| - | + | <ul> | |
| - | < | + | <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> |
| - | < | + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> |
| - | < | + | </ul> |
| + | </ul> | ||
| + | |||
| + | </td> | ||
| + | <td>23/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>24/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>25/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>26/09 | ||
| + | <ul> | ||
| + | <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li> | ||
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>27/09</td> | ||
| + | <td>28/09</td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>29/09 |
| - | + | <ul> | |
| - | < | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> |
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>30/09 | ||
| + | <ul> | ||
| + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
| + | <td></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
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| - | |||
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| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | < | + | |
| + | <thead><h2 align=center>October</h2> | ||
| + | |||
| + | </thead> | ||
| + | |||
| + | <tbody> | ||
<table style="width:100%"> | <table style="width:100%"> | ||
<tr> | <tr> | ||
| - | <th>Monday</th> | + | <th style="background-color:#bfacc0">Monday</th> |
| - | <th>Tuesday</th> | + | <th style="background-color:#bfacc0">Tuesday</th> |
| - | <th>Wednesday</th> | + | <th style="background-color:#bfacc0">Wednesday</th> |
| - | <th>Thursday</th> | + | <th style="background-color:#bfacc0">Thursday</th> |
| - | <th>Friday</th> | + | <th style="background-color:#bfacc0">Friday</th> |
| - | <th>Saturday</th> | + | <th style="background-color:#bfacc0">Saturday</th> |
| - | <th>Sunday</th> | + | <th style="background-color:#bfacc0">Sunday</th> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td></td> |
| - | < | + | <td></td> |
| - | < | + | <td>01/10 |
| - | < | + | <ul> |
| - | < | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> |
| + | <ul> | ||
| + | <li>Glycerol Stock</li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>02/10</td> | ||
| + | <td>03/10</td> | ||
| + | <td>04/10</td> | ||
| + | <td>05/10</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | < | + | <td>06/10 |
| - | < | + | <ul> |
| - | < | + | <li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>, |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and |
| - | < | + | <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li> |
| - | < | + | <ul> |
| - | < | + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> |
| + | </ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>07/10 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf" style="color:#f05151">Gel Purification</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>08/10 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | </td> | ||
| + | <td>09/10 | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li>Inoculum</li> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>10/10 | ||
| + | <ul> | ||
| + | <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and | ||
| + | <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li> | ||
| + | <ul> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </td> | ||
| + | <td>11/10</td> | ||
| + | <td>12/10</td> | ||
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>13/10</td> |
| - | < | + | <td>14/10</td> |
| - | < | + | <td>15/10</td> |
| - | < | + | <td>16/10</td> |
| - | < | + | <td>17/10</td> |
| - | < | + | <td>18/10</td> |
| - | < | + | <td>19/10</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>20/10</td> |
| - | < | + | <td>21/10</td> |
| - | < | + | <td>22/10</td> |
| - | < | + | <td>23/10</td> |
| - | < | + | <td>24/10</td> |
| - | < | + | <td>25/10</td> |
| - | < | + | <td>26/10</td> |
</tr> <tr> | </tr> <tr> | ||
| - | < | + | <td>27/10</td> |
| - | < | + | <td>28/10</td> |
| - | < | + | <td>29/10</td> |
| - | < | + | <td>30/10</td> |
| - | < | + | <td>31/10</td> |
| - | < | + | <td></td> |
| - | < | + | <td></td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 817: | Line 1,111: | ||
</body> | </body> | ||
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Latest revision as of 20:32, 17 October 2014
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