Team:ITESM-CEM/Safety

From 2014.igem.org

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     <td colspan="3" valign="bottom"><h3>ITESM-CEM | Medical Bioremediation</h3></td>
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     <td colspan="3" valign="bottom"><h3>ITESM-CEM | Enzy7-K me</h3></td>
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       <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Interlab</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Existing GFP device</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 1</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 2</a></sub>
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<h2>Overview</h2>
 
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      <p>During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study.
 
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The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.
 
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Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.
 
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The aim of this study is to report the relative efficiency of the following genetic devices:<br><br>
 
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1) Promoter BBa_K823005 in low-copy plasmid psB3K3<br>
 
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2) Promoter BBa_K823005 in high-copy plasmig psB1C3<br>
 
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3) Promoter BBa_K823012 in high-copy plasmid psB1C3<br><br>
 
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In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.<br><br>
 
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GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.
 
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<h2>Laboratory Biosafety </h2>
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</p>
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<a href="https://www.dropbox.com/s/mjyx0m9eh9xj1a7/Safety%20form.pdf?dl=0"><img src="https://static.igem.org/mediawiki/2014/5/56/Dany_safety_editado-1.jpg" align="right"  hspace="10" BORDER=10></a>
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<p style="text-align: justify; text-justify: inter-word;"> Our laboratory is classified as Laboratory of Risk 1. According to iGEM safety page and COFEPRIS which is the agency in Mexico that is in charged of regulating a variety of health related topics, a laboratory of risk level 1 is the one that can hold microorganisms that can't cause a disease in humans.<br>
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However in every laboratory biological control of experiments which involved microorganisms is needed. For every solid lab material that was previously in contact with microorganisms, such as Petri dishes, pipet tips, paper, etc., is placed on a special red container, which is then handled by a certified company in charge of managing biological wastes.<br> In the other hand, to maintain clean our work place after finishing using microorganisms we clean with ethanol 70%. We also use plastic gloves to avoid microbial contact with our skin. For liquid materials that were in contact with microbes, they are placed on an individual flask, so we can sterilize it later, followed by its proper storage. DNA gels which have SYBR safe are stored in a plastic bag and also disposed as dangerous material. </p>
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<h2>Laboratory Safety </h2>
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<p style="text-align: justify; text-justify: inter-word;"> Our laboratory not only possesses biosecurity standards but also safety normatives. We have a lab shower in case of chemical exposure with our bodies. We use special gloves when we need to handle cold and hot materials. It is important not to forget proper waste disposals of chemicals, such as acids, bases and any other chemical solutions. When looking bacteria that expresses fluorescent proteins, we use glasses to protect our eyes from UV light. When glass materials are broken we placed them into an special box for broken material. In case of fire we have at hand a fire extinguisher which is inspected every 6 months.<br><br>
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Now You know a little bit more about how we take care of us while we work. If you want to know further information <b>click on the image</b> to download our iGEM safety form.
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       <p>&nbsp;</p></td>
       <p>&nbsp;</p></td>

Latest revision as of 17:45, 17 October 2014

TEC-CEM | Safety

ITESM-CEM | Enzy7-K me

Safety 4102

 

Laboratory Biosafety

Our laboratory is classified as Laboratory of Risk 1. According to iGEM safety page and COFEPRIS which is the agency in Mexico that is in charged of regulating a variety of health related topics, a laboratory of risk level 1 is the one that can hold microorganisms that can't cause a disease in humans.
However in every laboratory biological control of experiments which involved microorganisms is needed. For every solid lab material that was previously in contact with microorganisms, such as Petri dishes, pipet tips, paper, etc., is placed on a special red container, which is then handled by a certified company in charge of managing biological wastes.
In the other hand, to maintain clean our work place after finishing using microorganisms we clean with ethanol 70%. We also use plastic gloves to avoid microbial contact with our skin. For liquid materials that were in contact with microbes, they are placed on an individual flask, so we can sterilize it later, followed by its proper storage. DNA gels which have SYBR safe are stored in a plastic bag and also disposed as dangerous material.

Laboratory Safety

Our laboratory not only possesses biosecurity standards but also safety normatives. We have a lab shower in case of chemical exposure with our bodies. We use special gloves when we need to handle cold and hot materials. It is important not to forget proper waste disposals of chemicals, such as acids, bases and any other chemical solutions. When looking bacteria that expresses fluorescent proteins, we use glasses to protect our eyes from UV light. When glass materials are broken we placed them into an special box for broken material. In case of fire we have at hand a fire extinguisher which is inspected every 6 months.

Now You know a little bit more about how we take care of us while we work. If you want to know further information click on the image to download our iGEM safety form.