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Team:UESTC-China
From 2014.igem.org
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<head> | <head> | ||
<meta http-equiv="Content-Type" content="text/html;charset=UTF-8" /> | <meta http-equiv="Content-Type" content="text/html;charset=UTF-8" /> | ||
| - | <title> | + | <title>Plant Vs HCHO</title> |
| + | |||
| + | |||
<style> | <style> | ||
| + | .imageRotation{ | ||
| + | height:500px; | ||
| + | width:1195px; | ||
| + | overflow:hidden; /*--超出容器的所有元素都不可见--*/ | ||
| + | position:relative; /*--相对定位--*/ | ||
| + | border:10px solid #eee; | ||
| + | bodrer-radius:5px; | ||
| + | -webkit-border-radius:5px; | ||
| + | -moz-border-radius:5px; | ||
| + | } | ||
| + | /*-------------图片容器---------------*/ | ||
| + | .imageBox{ | ||
| + | position:absolute; /*--固定定位--*/ | ||
| + | height:500px; | ||
| + | top:0px; | ||
| + | left:0px; | ||
| + | overflow:hidden; | ||
| + | } | ||
| + | .imageBox img { | ||
| + | display:block; | ||
| + | height:500px; | ||
| + | width:1195px; | ||
| + | float:left; | ||
| + | border:none; | ||
| + | } | ||
| + | /*-------------标题容器---------------*/ | ||
| + | .titleBox{ | ||
| + | position:absolute; | ||
| + | bottom:0px; | ||
| + | width:1214px; | ||
| + | height:40px; | ||
| + | overflow:hidden; | ||
| + | } | ||
| + | .titleBox p{ | ||
| + | position:absolute; | ||
| + | bottom:-40px; | ||
| + | width:1214px; | ||
| + | height:40px; | ||
| + | margin:0px; | ||
| + | padding:0px 10px; | ||
| + | line-height:40px; | ||
| + | z-index:1; | ||
| + | background-color:#000; | ||
| + | color:#fff; | ||
| + | font-family:"微软雅黑","yahei",calibri, arial, helvetica, sans-serif,Italic; | ||
| + | font-weight: bold; | ||
| + | font-style: sans-serif; | ||
| + | opacity:0.5; | ||
| + | -moz-opacity:0.5; | ||
| + | -webkit-opacity:0.5; | ||
| + | filter:alpha(opacity=50); | ||
| + | } | ||
| + | .titleBox p span{ | ||
| + | opacity:1; | ||
| + | -moz-opacity:1; | ||
| + | -webkit-opacity:1; | ||
| + | filter:alpha(opacity=100); | ||
| + | } | ||
| + | .titleBox p.active{ | ||
| + | bottom:0px; | ||
| + | } | ||
| + | /*-------------图标容器---------------*/ | ||
| + | .icoBox{ | ||
| + | position:absolute; /*--固定定位--*/ | ||
| + | bottom:14px; | ||
| + | right:15px; | ||
| + | width:76px; | ||
| + | height:12px; | ||
| + | text-align:center; | ||
| + | line-height:40px; | ||
| + | z-index:2; | ||
| + | } | ||
| + | .icoBox span{ | ||
| + | display:block; | ||
| + | float:left; | ||
| + | height:12px; | ||
| + | width:12px; | ||
| + | margin-left:3px; | ||
| + | overflow:hidden; | ||
| + | background:url("https://static.igem.org/mediawiki/2014/a/a2/Ico.png") 0px 0px no-repeat; | ||
| + | cursor:pointer; | ||
| + | } | ||
| + | .icoBox span.active { | ||
| + | background-position:0px -12px; | ||
| + | cursor:default; | ||
| + | } | ||
| + | |||
| + | #content { | ||
| + | position: relative; | ||
| + | width: 1215px; | ||
| + | # margin-left: 10px; | ||
| + | margin: 0 auto; | ||
| + | padding: 5px 5px 5px 5px;; | ||
| + | background: white; | ||
| + | color: black; | ||
| + | border-left: 1px solid #444444; | ||
| + | border-right: 1px solid #444444; | ||
| + | line-height: 1.5em; | ||
| + | z-index: 2; | ||
| + | } | ||
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#header{ | #header{ | ||
height: 280px; | height: 280px; | ||
| - | background-image:url("https://static.igem.org/mediawiki/2014/ | + | background-image:url("https://static.igem.org/mediawiki/2014/4/49/New_New_banner.jpg");/* yan change to plant-vs HCHO*/ |
background-position: top center; | background-position: top center; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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} | } | ||
| + | |||
@font-face { | @font-face { | ||
font-family: ubuntu; | font-family: ubuntu; | ||
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.top-menu-each{ | .top-menu-each{ | ||
height: 63px; | height: 63px; | ||
| + | |||
font-family: helveticallt,"微软雅黑"; | font-family: helveticallt,"微软雅黑"; | ||
color: white; | color: white; | ||
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float: left; | float: left; | ||
cursor: pointer; | cursor: pointer; | ||
| - | font-weight: bold; | + | font-weight: bold; |
} | } | ||
| Line 86: | Line 190: | ||
.arrow{ | .arrow{ | ||
float: left; | float: left; | ||
| - | background-image: url("http://fudanigem.phx-2.ifreeurl.com/img/arrow.png"); | + | background-image: url("http://fudanigem.phx-2.ifreeurl.com/img/arrow.png");/*needn't change*/ |
line-height: 63px; | line-height: 63px; | ||
width: 12px; | width: 12px; | ||
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padding-left: 35px; | padding-left: 35px; | ||
padding-right: 10px; | padding-right: 10px; | ||
| - | background-image: url('http://fudanigem.phx-2.ifreeurl.com/img/big-photo-hover.png'); | + | background-image: url('http://fudanigem.phx-2.ifreeurl.com/img/big-photo-hover.png');/*no change*/ |
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
margin-top: -130px; | margin-top: -130px; | ||
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font-size: 30px; | font-size: 30px; | ||
line-height: 30px; | line-height: 30px; | ||
| - | font-family: | + | font-family: helveticalmd,"微软雅黑"; |
font-weight: bold; | font-weight: bold; | ||
padding-top: 25px; | padding-top: 25px; | ||
| Line 210: | Line 314: | ||
height: 180px; | height: 180px; | ||
| - | background-color: | + | background-color:white; |
border-top-left-radius:8px; | border-top-left-radius:8px; | ||
border-top-right-radius:8px; | border-top-right-radius:8px; | ||
| - | margin-top: | + | margin-top: 20px; |
} | } | ||
#main-content{ | #main-content{ | ||
| Line 229: | Line 333: | ||
height: 60px; | height: 60px; | ||
background-color: #394264; | background-color: #394264; | ||
| - | font-family: | + | font-family: ubuntu,"宋体"; |
font-size: 18px; | font-size: 18px; | ||
line-height: 60px; | line-height: 60px; | ||
| Line 254: | Line 358: | ||
#middle-photo{ | #middle-photo{ | ||
float: left; | float: left; | ||
| - | margin-bottom: | + | margin-bottom: 445px; |
} | } | ||
.middle-photo-each{ | .middle-photo-each{ | ||
| - | padding: | + | padding:9px 9px 9px 9px; |
background-color: white; | background-color: white; | ||
margin-bottom: 15px; | margin-bottom: 15px; | ||
| - | margin-left: | + | margin-left: 250px; |
} | } | ||
| Line 277: | Line 381: | ||
color: black; | color: black; | ||
font-size: 16px; | font-size: 16px; | ||
| - | font-family: | + | font-family: "微软雅黑"; |
line-height: 20px; | line-height: 20px; | ||
} | } | ||
| Line 291: | Line 395: | ||
} | } | ||
#top{ | #top{ | ||
| - | + | background-image: url('https://static.igem.org/mediawiki/2014/8/89/Uestc_top2.png'); | |
| - | + | width: 65px; | |
| - | + | height: 138px; | |
| - | + | position: fixed; | |
| - | + | top: 70%; | |
| - | + | margin-left: 1200px; | |
| - | + | cursor: pointer; | |
| + | z-index: 999; | ||
| + | |||
} | } | ||
#logo{ | #logo{ | ||
position: absolute; | position: absolute; | ||
| - | + | ||
width: 154px; | width: 154px; | ||
height: 147px; | height: 147px; | ||
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color:white; | color:white; | ||
} | } | ||
| + | #intro1{ | ||
| + | font-family: helveticalmd,"微软雅黑"; | ||
| + | font-size: 50px; | ||
| + | margin-top: 20px; | ||
| + | margin-left: 10px; | ||
| + | color: white; | ||
| + | } | ||
| + | #intro2{ | ||
| + | font-family: helveticalmd,"微软雅黑"; | ||
| + | font-size: 23px; | ||
| + | margin-left: 10px; | ||
| + | margin-right: 10px; | ||
| + | color:white; | ||
| + | text-align:justify; | ||
| + | text-justify:inter-ideograph; | ||
| + | text-indent:2em; | ||
| + | } | ||
| + | #introduction{ | ||
| + | background-color:#1bbc9b | ||
| + | } | ||
| + | /*rocket-to-top*/ | ||
| + | *{list-style:none;border:0;} | ||
| - | # | + | #rocket-to-top div { |
| + | left: 0; | ||
| + | margin: 0; | ||
| + | overflow: hidden; | ||
| + | padding: 0; | ||
| + | position: absolute; | ||
| + | top: 0; | ||
| + | width: 149px; | ||
| + | } | ||
| + | #rocket-to-top .level-2 { | ||
| + | background: url("https://static.igem.org/mediawiki/2014/a/a6/UestcUp.png") no-repeat scroll -149px 0 transparent; | ||
| + | display: none; | ||
| + | height: 250px; | ||
| + | opacity: 0; | ||
| + | z-index: 1; | ||
| + | } | ||
| + | #rocket-to-top .level-3 { | ||
| + | background: none repeat scroll 0 0 transparent; | ||
| + | cursor: pointer; | ||
| + | display: block; | ||
| + | height: 150px; | ||
| + | z-index: 2; | ||
| + | } | ||
| + | #rocket-to-top { | ||
| + | background: url("https://static.igem.org/mediawiki/2014/a/a6/UestcUp.png") no-repeat scroll 0 0 transparent; | ||
| + | cursor: default; | ||
| + | display: block; | ||
| + | height: 250px; | ||
| + | margin: -125px 0 0; | ||
| + | overflow: hidden; | ||
| + | padding: 0; | ||
| + | position: fixed; | ||
| + | right: 0; | ||
| + | top: 80%; | ||
| + | width: 149px; | ||
| + | z-index: 11; | ||
| + | } | ||
| + | /*rocket-to-top end*/ | ||
| + | #intro2 img{ | ||
| + | margin:0px 0px 0px -10px; | ||
| + | } | ||
| + | </style> | ||
| + | <script type="text/javascript" src="chrome-extension://bfbmjmiodbnnpllbbbfblcplfjjepjdn/js/injected.js"></script><style>@-moz-keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}@-webkit-keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}@-o-keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}@keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}embed,object{animation-duration:.001s;-ms-animation-duration:.001s;-moz-animation-duration:.001s;-webkit-animation-duration:.001s;-o-animation-duration:.001s;animation-name:nodeInserted;-ms-animation-name:nodeInserted;-moz-animation-name:nodeInserted;-webkit-animation-name:nodeInserted;-o-animation-name:nodeInserted;}</style> | ||
| + | </head> | ||
| + | <body> | ||
| + | <div id="total-wrap"> | ||
| + | <div id="header"> | ||
| + | <div id="emp"> | ||
| + | <div id='logo'><a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/d/da/NewLogo2.png"></a></div> | ||
| + | </div> | ||
| + | <div id="top-menu"> | ||
| + | <div class="top-menu-each"> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China"><p>Home</p></a> | ||
| + | </div> | ||
| + | <div class="top-menu-each"> | ||
| + | <div class="arrow"></div> | ||
| + | <p>Team</p> | ||
| + | <div class="top-menu-sub"> | ||
| + | <ul> | ||
| - | . | + | <a href="https://2014.igem.org/Team:UESTC-China/Team"><li>Members</li></a> |
| - | . | + | <a href="https://2014.igem.org/Team:UESTC-China/Attribution"><li>Attribution</li></a> |
| + | <a href="https://igem.org/Team.cgi?year=2014&team_name=UESTC-China"><li>Team Profile</li></a> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China/Notebook"><li>Notebook</li></a> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
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| - | + | <div class="top-menu-each"> | |
| - | + | <div class="arrow"></div> | |
| - | + | <p>Project</p> | |
| - | top | + | <div class="top-menu-sub"> |
| - | + | <ul> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Project"><li>Overview</li></a> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Design"><li>Design</li></a> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/result"><li>Result</li></a> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Futurework"><li>Future work</li></a> | |
| + | <a href="https://2014.igem.org/Team:UESTC-China/Judingform"><li>Judging Criteria</li></a> | ||
| - | + | </ul> | |
| - | + | </div> | |
| - | top | + | </div> |
| - | + | <div class="top-menu-each"> | |
| - | + | <div class="arrow"></div> | |
| - | + | <p>Modeling</p> | |
| - | + | <div class="top-menu-sub"> | |
| - | + | <ul> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Modeling"><li>Overview</li></a> | |
| + | <a href="https://2014.igem.org/Team:UESTC-China/Modeling1"><li>HPS/PHI</li></a> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China/Modeling2"><li>FALDH/FDH</li></a> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China/Modeling3"><li>AtAHA2</a> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="top-menu-each"> | ||
| + | <div class="arrow"></div> | ||
| + | <p>Parts</p> | ||
| + | <div class="top-menu-sub"> | ||
| + | <ul> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China/BioBrick"><li>BioBrick</li></a> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China/Protocol"><li>Protocol</li></a> | ||
| - | + | </ul> | |
| - | + | </div> | |
| - | top | + | </div> |
| - | + | <div class="top-menu-each"> | |
| - | + | <div class="arrow"></div> | |
| - | + | <p>Human Practice</p> | |
| - | + | <div class="top-menu-sub"> | |
| - | + | <ul> | |
| + | <a href="https://2014.igem.org/Team:UESTC-China/Questionnaire.html"><li>Questionnaire</li></a> | ||
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Knowledgequiz"><li>Knowledge quiz</li></a> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Lecture.html"><li>Lecture</li></a> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Communication.html"><li>Communication</li></a> | |
| - | + | <a href="https://2014.igem.org/Team:UESTC-China/Art"><li>Art</li></a> | |
| - | + | </ul> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | ||
| - | + | <div class="top-menu-each"> | |
| + | <div class="arrow"></div> | ||
| + | <p>Safety</p> | ||
| + | <div class="top-menu-sub"> | ||
| + | <ul> | ||
| + | <a href="https://2014.igem.org/Team:UESTC-China/Safety"><li>Safety</li></a> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
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</div> | </div> | ||
| - | + | </div> | |
| - | + | <div class="imageRotation"> | |
| - | + | <div class="imageBox" style="width: 1214px; left: 0px;"> | |
| - | + | <div><img src="https://static.igem.org/mediawiki/2014/2/21/All_students1.jpg"></div> | |
| - | + | <div><img src="https://static.igem.org/mediawiki/2014/1/15/NewPlant1.jpg"></div> | |
| - | + | <div><img src="https://static.igem.org/mediawiki/2014/8/83/Home1.jpg"></div> | |
| - | + | <div><img src="https://static.igem.org/mediawiki/2014/8/8b/Xiaojiejie.jpg"></div> | |
| - | + | <div><img src="https://static.igem.org/mediawiki/2014/c/ce/Humanpractice.jpg"></div> | |
| - | + | <!--<div><img src="https://static.igem.org/mediawiki/2014/1/14/All_students3.jpg"></div>--> | |
| - | + | </div> | |
| - | + | <div class="titleBox"> | |
| - | + | <p class="active" style="bottom: 0px;"><span>Our Team: Brilliant Guys</span></p> | |
| - | + | <p style="bottom: -40px;">Plants: Transgenic Tobaccos</p> | |
| - | + | <p style="bottom: -40px;">Super Plant: Plants vs HCOH</p> | |
| - | + | <p style="bottom: -40px;">Safety: Operation</p> | |
| - | + | <p style="bottom: -40px;">Human Practice</p> | |
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</div> | </div> | ||
| - | + | <div class="icoBox"> | |
| - | + | <span class="active" rel="1">1</span> | |
| - | + | <span rel="2" class="">2</span> | |
| - | + | <span rel="3" class="">3</span> | |
| - | + | <span rel="4" class="">4</span> | |
| - | + | <span rel="5" class="">5</span> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <div id="introduction"> | |
| - | + | <p id="intro1"><b>Introduction</b></p> | |
| - | + | <br/> | |
| - | + | <p id="intro2" style="color:white;text-indent: 0em"> | |
| - | + | With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively. | |
| - | + | <br/><br/> | |
| - | + | To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively. | |
| - | + | <br/><br/> | |
| - | + | After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant. | |
| - | + | <br/><br/> | |
| - | + | To further increase the degradation efficiency of formaldehyde, we co-expressed gene <i>AHA2</i>, which is original from <i>Arabidopsis thaliana</i>, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum. | |
| - | + | <br/><br/> | |
| - | + | With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments. | |
| - | + | <br/><br/> | |
| - | + | ||
| - | + | <img style="height:300px;width:1215px" src="https://static.igem.org/mediawiki/2014/8/89/Jiantu5.jpg" alt="pic"> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
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</p> | </p> | ||
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</div> | </div> | ||
| + | |||
| + | <div id="footer"> | ||
| + | |||
| + | <img src='https://static.igem.org/mediawiki/2014/3/32/Foot6.jpg' /> | ||
| + | |||
| + | |||
</div> | </div> | ||
| + | </div> | ||
| + | |||
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Latest revision as of 15:14, 17 October 2014
Our Team: Brilliant Guys
Plants: Transgenic Tobaccos
Super Plant: Plants vs HCOH
Safety: Operation
Human Practice
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Introduction
With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.
To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively.
After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.
To further increase the degradation efficiency of formaldehyde, we co-expressed gene AHA2, which is original from Arabidopsis thaliana, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.
With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.
