Team:Oxford/realisation bioremediation

From 2014.igem.org

(Difference between revisions)
 
(16 intermediate revisions not shown)
Line 9: Line 9:
<div style="background-color:#D9D9D9; opacity:0.7; z-index:5; Height:75px; width:100%;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;margin-top:10px;">
<div style="background-color:#D9D9D9; opacity:0.7; z-index:5; Height:75px; width:100%;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;margin-top:10px;">
-
<div style="background-color:white; opacity:0.9; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#00000; font-weight: 450;"><br><center><font style="opacity:0.7">Realisation</font></center></div>
+
<div style="background-color:white; opacity:0.9; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#00000; font-weight: 450;"><br><center><font style="opacity:0.7">Bioremediation Realisation</font></center></div>
</div>
</div>
<br>
<br>
Line 23: Line 23:
<div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-50px;">
<div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-50px;">
<a href="#"><img src="https://static.igem.org/mediawiki/2014/5/50/OxigemLabbook.png" style="position:absolute;width:6%;margin-left:84%;margin-top:-13%;z-index:10;"></a>
<a href="#"><img src="https://static.igem.org/mediawiki/2014/5/50/OxigemLabbook.png" style="position:absolute;width:6%;margin-left:84%;margin-top:-13%;z-index:10;"></a>
-
<a href="#"><img src="https://static.igem.org/mediawiki/2014/a/a4/OxigemProtocols.png" style="position:absolute;width:6%;margin-left:91%;margin-top:-13%;z-index:10;"></a>
+
<a href="https://static.igem.org/mediawiki/2014/1/16/Oxigem_LAB_PROTOCOLS.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a4/OxigemProtocols.png" style="position:absolute;width:6%;margin-left:91%;margin-top:-13%;z-index:10;"></a>
<div style="position:absolute;background-color:rgba(255,255,255,0.6);border-radius:15px; z-index:5;margin-top:-18.2%; Height:70px; width:20%;font-size:65px;font-family:Helvetica; font-weight: 450;padding-left:10px;padding-right:10px;padding-top:3px;min-width:170px;margin-bottom:3px;">
<div style="position:absolute;background-color:rgba(255,255,255,0.6);border-radius:15px; z-index:5;margin-top:-18.2%; Height:70px; width:20%;font-size:65px;font-family:Helvetica; font-weight: 450;padding-left:10px;padding-right:10px;padding-top:3px;min-width:170px;margin-bottom:3px;">
Line 29: Line 29:
<div style="width:100%;"><font style="font-size:15px;font-weight:500;">Show all:</font></div>
<div style="width:100%;"><font style="font-size:15px;font-weight:500;">Show all:</font></div>
-
<a href="#showmodelling""><div class="orange_news_block1 showmodelling" style="background: #F9A7B0;border-radius:15px;color:black;float:left;height:40%;width:40%;margin-left:6%;padding-top:5%;margin-top:3px;"><center>
+
<a href="#showmodelling"><div class="orange_news_block1 showmodelling" style="background: #F9A7B0;border-radius:15px;color:black;float:left;height:40%;width:40%;margin-left:6%;padding-top:10px;"><center>
-
<h1white><font style="font-size:15px;font-weight:500;">Modelling</font>
+
<h1white><font style="font-size:15px;font-weight:500;">Modelling</font></h1white></center>
</div></a>
</div></a>
-
<a href="#showwetlab"><div class="orange_news_block1 showwetlab" style="background: #ADD8E6;border-radius:15px;color:black;float:left;height:40%;width:40%;padding-top:5%;margin-left:3%;margin-top:3px;"><center>
+
<a href="#showwetlab"><div class="orange_news_block1 showwetlab" style="background: #ADD8E6;border-radius:15px;color:black;float:left;height:40%;width:40%;margin-left:3%;padding-top:10px;"><center>
-
<h1white><font style="font-size:15px;font-weight:500;">Wetlab</font>
+
<h1white><font style="font-size:15px;font-weight:500;">Wetlab</font></h1white></center>
</div></a>
</div></a>
Line 40: Line 40:
<br>
<br>
-
<h1>Introduction about the realisation of the bioremediation</h1>
+
<h1>Introduction</h1>
-
Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation Lots and lots of info about Realisation
+
We thought that it was important to think properly about how the system would be implemented in reality and to keep that in mind throughout the whole project. This meant that we could keep our research constantly focused on the end product.
<br><br>
<br><br>
 +
As such,  we designed the complete system on the CAD software <a href="http://www.solidworks.co.uk/">SolidWorks</a>. This enabled us to demonstrate how the project could actually be incorporated into the real world. It also allowed us to overcome flaws in the design before we started construction. Pictures taken from <a href="http://www.solidworks.co.uk/">SolidWorks</a> are shown below. In addition to this, we used 3D printing to <a href="https://2014.igem.org/Team:Oxford/biosensor_realisation">construct the biosensor part of the design</a>.<br><br>
</div>
</div>
 +
<div class="orange_news_block">
 +
<h1white>The complete design of the system</h1white>
 +
</div>
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<head>
 
-
 
-
<div class="row">
 
-
<a href="#show1" class="show" id="show1"><div class="orange_news_block1">
 
-
<h1white>section1</h1white>
 
-
<img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" />
 
-
</div></a>
 
-
 
-
<a href="#hide1" class="hide" id="hide1"><div class="orange_news_block1">
 
-
<h1white>section1 </h1white></div></a>
 
-
<div class="list">
 
<div class="white_news_block">
<div class="white_news_block">
 +
<img src="https://static.igem.org/mediawiki/2014/9/9c/Oxford_build6.png" style="float:right;position:relative; width:100%;margin-bottom:2%;" />
 +
<br><br>
 +
1. Insert a cartridge into the empty container, then open the lid.
 +
<br><br>
 +
2. In a fume cupboard/with appropriate apparatus, add your chlorinated waste to the container. Observe from the measurement markings on the container how much you’ve added. Then, read from the chart provided, how much water and buffer solution to add to the container.
 +
<br><br>
 +
3. Add this water and buffer solution to the container. From the viewing panel, you should be able to observe the chlorinated waste separating out into a clear layer below the aqueous layer. Then, add the bioremediation beads that contain the bacteria. To find out more about these beads, click on the relevant section in the realisation section.
 +
<br><br>
 +
4. Close the lid firmly ensuring that the system stays airtight. Leave the system until the biosensor indicates that the contents of the container are safe to pour down the drain. We are working towards getting this safe level to be around 5ppm, the maximum level of dichloromethane in drinking water for it to be considered drinkable.
</div>
</div>
-
</div>
 
-
</div>
 
-
 
-
 
-
 
-
<div class="row">
 
-
<a href="#show2" class="show" id="show2"><div class="orange_news_block1">
 
-
<h1white>section2</h1white>
 
-
<img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" />
 
-
</div></a>
 
-
 
-
<a href="#hide2" class="hide" id="hide2"><div class="orange_news_block1">
 
-
<h1white>section2</h1white></div></a>
 
-
<div class="list">
 
<div class="white_news_block">
<div class="white_news_block">
 +
<a href="https://2014.igem.org/Team:Oxford/bioremediation">
 +
<img src="https://static.igem.org/mediawiki/2014/1/11/Oxford_bioremediation_title.png" style="float:left;position:relative;width:50%; margin-top:2%;margin-left:25%;margin-right:25%;" /></a>
 +
<a href="https://2014.igem.org/Team:Oxford/Modelling"><img src="https://static.igem.org/mediawiki/2014/6/6e/Oxford_modelling_homepage_link.png" style="float:left;position:relative; width:50%; margin-top:2%;margin-left:25%;margin-right:25%;" /></a>
-
</div>
+
<img src="https://static.igem.org/mediawiki/2014/d/d6/Oxford_Realisation_pages.png" style="float:left;position:relative; width:50%; margin-top:2%;margin-left:25%;margin-right:25%;" />
 +
<a href="https://2014.igem.org/Team:Oxford/biosensor_realisation"><img src="https://static.igem.org/mediawiki/2014/c/cd/Oxford_Biosensor_realisation.png" style="float:left;position:relative; width:20%; margin-top:2%;margin-left:10%;margin-right:0%;" /></a>
-
</div>
+
<a href="https://2014.igem.org/Team:Oxford/realisation_bioremediation"><img src="https://static.igem.org/mediawiki/2014/a/a7/Oxford_Bioremediation_realisation.png" style="float:left;position:relative; width:20%; margin-top:2%;margin-left:10%;margin-right:10%;" /></a>
-
</div>
+
 +
<a href="https://2014.igem.org/Team:Oxford/biopolymer_containment"><img src="https://static.igem.org/mediawiki/2014/0/07/Oxford_Biopolymer_containment.png" style="float:left;position:relative; width:20%; margin-top:2%;margin-left:0%;margin-right:10%;" /></a>
-
 
+
<br><br><br>
-
 
+
Oxford iGEM 2014
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<div class="row">
+
-
<a href="#show3" class="show" id="show3"><div class="orange_news_block1">
+
-
<h1white>section3</h1white>
+
-
<img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" />
+
-
</div></a>
+
-
 
+
-
<a href="#hide3" class="hide" id="hide3"><div class="orange_news_block1">
+
-
<h1white>section3</h1white></div></a>
+
-
<div class="list">
+
-
<div class="white_news_block">
+
-
 
+
</div>
</div>
 +
<br>
-
</div>
 
-
</div>
 
Line 142: Line 106:
-
 
-
<div class="white_news_block">
 
-
<a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation"><img src="https://static.igem.org/mediawiki/2014/c/ca/Oxford_Characterisation.png" style="float:left;position:relative; width:23%;" /></a>
 
-
 
-
<a href="https://2014.igem.org/Team:Oxford/biosensor_realisation"><img src="https://static.igem.org/mediawiki/2014/4/49/Oxford_Realisation_dark.png" style="float:right;position:relative; width:23%;" /></a>
 
-
 
-
<a href="https://2014.igem.org/Team:Oxford/biosensor_construction"><img src="https://static.igem.org/mediawiki/2014/a/ae/Oxford_construction.png" style="float:left;position:relative; width:23%; margin-left: 2.66%" /></a>
 
-
 
-
<a href="https://2014.igem.org/Team:Oxford/biosensor_optimisation"><img src="https://static.igem.org/mediawiki/2014/9/93/Oxford_Optimisation.png" style="float:right;position:relative; width:23%; margin-right: 2.66%" /></a>
 
-
 
-
<a href="https://2014.igem.org/Team:Oxford/biosensor"><img src="https://static.igem.org/mediawiki/2014/a/a7/Oxford_biosensor_link.png" style="float:left;position:relative; width:100%; margin-top:2%" /></a>
 
-
 
-
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
-
</div>
 

Latest revision as of 16:48, 17 October 2014


Bioremediation Realisation


Introduction

We thought that it was important to think properly about how the system would be implemented in reality and to keep that in mind throughout the whole project. This meant that we could keep our research constantly focused on the end product.

As such, we designed the complete system on the CAD software SolidWorks. This enabled us to demonstrate how the project could actually be incorporated into the real world. It also allowed us to overcome flaws in the design before we started construction. Pictures taken from SolidWorks are shown below. In addition to this, we used 3D printing to construct the biosensor part of the design.

The complete design of the system


1. Insert a cartridge into the empty container, then open the lid.

2. In a fume cupboard/with appropriate apparatus, add your chlorinated waste to the container. Observe from the measurement markings on the container how much you’ve added. Then, read from the chart provided, how much water and buffer solution to add to the container.

3. Add this water and buffer solution to the container. From the viewing panel, you should be able to observe the chlorinated waste separating out into a clear layer below the aqueous layer. Then, add the bioremediation beads that contain the bacteria. To find out more about these beads, click on the relevant section in the realisation section.

4. Close the lid firmly ensuring that the system stays airtight. Leave the system until the biosensor indicates that the contents of the container are safe to pour down the drain. We are working towards getting this safe level to be around 5ppm, the maximum level of dichloromethane in drinking water for it to be considered drinkable.



Oxford iGEM 2014

Retrieved from "http://2014.igem.org/Team:Oxford/realisation_bioremediation"