Team:HIT-Harbin/Project

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       <h2>Project</h2>
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                <h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="B-POM: " style="width: 59px; height: 18px;"><cufontext>B-POM: </cufontext></cufon><cufon class="cufon cufon-canvas" alt="Biological " style="width: 90px; height: 18px;"><cufontext>Biological </cufontext></cufon><cufon class="cufon cufon-canvas" alt="proportional " style="width: 116px; height: 18px;"><cufontext>proportional </cufontext></cufon><cufon class="cufon cufon-canvas" alt="operational " style="width: 105px; height: 18px;"><cufontext>operational </cufontext></cufon><cufon class="cufon cufon-canvas" alt="Mu-circuit" style="width: 90px; height: 18px;"><cufontext>Mu-circuit</cufontext></cufon></span></h5>
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<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="Overview: " style="width: 59px; height: 18px;"><cufontext>Abstract </cufontext></cufon></span></h5>
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                    <p>Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.</p>
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                    <p>In 1990s, Eric Mjolsness and several researchers developed the concept of genetic circuit from electronic circuits, to facilitate research in modeling gene expression and regulation (Mjolsness, Sharp et al. 1991, McAdams and Shapiro 1995, Reinitz and Sharp 1996, Sharp and Reinitz 1998). Upon the inspiration, Ron Weiss, a computer engineer of MIT, constructed an AND-gate genetic circuit in 2001 (Weiss, Knight et al. 2001). Based on Weiss’ pioneering result, the team of Jeff Hasty took IPTG (isopropyl β-D-1-Thiogalactopyranoside) and aTc (anhydrotetracycline) as the inputs and GFP (green fluorescent protein) as the output; demonstrating the AND-gate circuit in a more lucid and applicable way (Hasty, McMillen et al. 2002). In 2005, to investigate the prospect of genetic circuit, Oliver Rackham proved that other kinds of logic circuits are feasible by orthogonal topological structures (Rackham and Chin 2005). In 2013 iGEM, we inherit the idea of resembling electronic circuits. By incorporating AND/OR gate and operational amplifier into one circuit, we create our device-Biological Proportional Operational Mu-circuit (B-POM).
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<p>So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings.
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In comparison with Weiss’ invention, B-POM has only one homogeneous or heterogeneous input and output. Besides, multiple magnitudes of input are distinguishable by the new circuit, and a given input corresponds to a certain output. Moreover, whereas general genetic circuits are vulnerable to the noise of cellular background, B-POM seems more interference-free, implicating the prominent stability of our device.
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</p><p>In applications, we plan to enhance Bio-electric Interface, the device designed by 2012 Edinburg iGEM team. If the input is biochemical signal molecules and the output become electrons, B-POM can be coupled with Bio-electric Interface, that ephemeral processes in cells will be precisely measurable by simple electronic methods and analyzable by computer. B-POM is also helpful in yoghurt producing, where the control of pH is inaccurate. When B-POM is transplanted into yoghurt-producing bacteria, let hydrogen ions be the input and lacR be the output, and select the proper B-POM parameters, then the pH will be steady around 5.5. In short, our inspired and reliable device is promising in various fields.
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      <h2>Reference:</h2>
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<p>1.Mimura J, Fujii-Kuriyama Y. Functional role of AhR in the expression of toxic effects by TCDD[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2003, 1619(3): 263-268.
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</p>
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<p>2.Denison M S, Heath-Pagliuso S. The Ah receptor: a regulator of the biochemical and toxicological actions of structurally diverse chemicals[J]. Bulletin of environmental contamination and toxicology, 1998, 61(5): 557-568.
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</p>
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<p>3.Toren A, Segal G, Ron E Z, et al. Structure–function studies of the recombinant protein bioemulsifier AlnA[J]. Environmental microbiology, 2002, 4(5): 257-261.
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</p>
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<p>4.Furukawa K, Fujihara H. Microbial degradation of polychlorinated biphenyls: biochemical and molecular features[J]. Journal of bioscience and bioengineering, 2008, 105(5): 433-449.
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</p>
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<p>5.Whitelaw M L, Göttlicher M, Gustafsson J A, et al. Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor[J]. The EMBO journal, 1993, 12(11): 4169.</p>
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Latest revision as of 01:47, 18 October 2014

Project

Abstract

Dioxins, by-products of various industrial processes, are commonly regarded as highly toxic compounds that are environmental pollutants and persistent organic pollutants. Its deleterious hazards intensify with the odorless, colorless and fat-soluble properties, which enable dioxins to accumulate in vivo thus threaten almost the whole biosphere. Considering their ablilty of causing reproductive and developmental problems, damaging the immune system, interfering with hormones and also leading to cancer, hand in glove with the difficulty of being tested, dioxins are worthy of the name “poison of the century”.

So as to detect dioxins blisteringly, we have constructed device for rapid detection of dioxins inside yeast. We utlized associability between the gene for testing dioxins of MOUSE as receptor and dioxins as antibodies, together with the regulation conducted by lexA operator to downstream promoters so that mdr and lexA are combined, and, striding a step further, induce downstream gene with the presence of dioxins. Eventually, we can achieve our goal of detecting dioxins rapidly through the system of positive feedback of flourescin. Concerning the properties of yeast, like non-toxic and able to survive in hypertonic environment, our project can be put into wide application in the field like food detection, hence guarantee the food safety among the community of we human beings.

Reference:

1.Mimura J, Fujii-Kuriyama Y. Functional role of AhR in the expression of toxic effects by TCDD[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2003, 1619(3): 263-268.

2.Denison M S, Heath-Pagliuso S. The Ah receptor: a regulator of the biochemical and toxicological actions of structurally diverse chemicals[J]. Bulletin of environmental contamination and toxicology, 1998, 61(5): 557-568.

3.Toren A, Segal G, Ron E Z, et al. Structure–function studies of the recombinant protein bioemulsifier AlnA[J]. Environmental microbiology, 2002, 4(5): 257-261.

4.Furukawa K, Fujihara H. Microbial degradation of polychlorinated biphenyls: biochemical and molecular features[J]. Journal of bioscience and bioengineering, 2008, 105(5): 433-449.

5.Whitelaw M L, Göttlicher M, Gustafsson J A, et al. Definition of a novel ligand binding domain of a nuclear bHLH receptor: co-localization of ligand and hsp90 binding activities within the regulable inactivation domain of the dioxin receptor[J]. The EMBO journal, 1993, 12(11): 4169.

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