"
Team:Evry/Interlab Study/09-17-2014
From 2014.igem.org
(Difference between revisions)
| (13 intermediate revisions not shown) | |||
| Line 20: | Line 20: | ||
<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | <br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | ||
<br/> | <br/> | ||
| - | + | Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C. | |
| - | + | <br> | |
| Line 33: | Line 33: | ||
<i>PSB1C3+E0240+J23100</i>: <br> | <i>PSB1C3+E0240+J23100</i>: <br> | ||
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).<br> | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).<br> | ||
| - | < | + | <FONT color="red">OVERFLOW</FONT> <br> |
<br> | <br> | ||
| + | |||
| + | <i>PSB1C3+E0240+J23101</i>: <br> | ||
| + | Measure of the cells' growth and fluorecence on TECAN infiniteM200.<br> | ||
| + | <FONT color="red">TECAN FAILED</FONT> <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23103</i>: <br> | <i>PSB1C3+E0240+J23103</i>: <br> | ||
| - | Measure of the cells' growth and fluorecence on TECAN infiniteM200 ( | + | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).<br> |
| - | <img src="" alt="PLAN DE PLAQUE" /> | + | <img src="https://static.igem.org/mediawiki/2014/6/65/PlaqueLeft.png" alt="PLAN DE PLAQUE" /> |
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23105</i>: <br> | <i>PSB1C3+E0240+J23105</i>: <br> | ||
| - | pre-culture of the colony | + | pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.<br> |
| + | <br> | ||
<i>PSB1C3+E0240+J23107</i>: <br> | <i>PSB1C3+E0240+J23107</i>: <br> | ||
| - | pre-culture of the colony | + | pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.<br> |
| + | <br> | ||
<i>PSB1C3+E0240+J23110</i>: <br> | <i>PSB1C3+E0240+J23110</i>: <br> | ||
| - | + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | |
| + | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23115</i>: <br> | <i>PSB1C3+E0240+J23115</i>: <br> | ||
| - | Measure of the cells' growth and fluorecence on TECAN infiniteM200 ( | + | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (12h-19h).<br> |
| - | <img src="" alt="PLAN DE PLAQUE" /> | + | <img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="PLAN DE PLAQUE" /> |
<br> | <br> | ||
<br> | <br> | ||
| + | |||
| + | PSB1C3+E0240+J23104, PSB1C3+E0240+J23112, PSB1C3+E0240+J23113: | ||
| + | PCR colony of 6 colonies for each construction following protocol table 1 and 2.<br> | ||
| + | Migration of the PCR products<br> | ||
| + | PCR purification of clones with the good construction <br> | ||
| + | |||
| + | |||
| + | <div class="center"> | ||
| + | <div class="thumb tnone"> | ||
| + | <div class="thumbinner" style="width:502px;"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3f/110.112.113.jpg" class="image"> | ||
| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/3/3f/110.112.113.jpg" width="450px;" class="thumbimage"/> | ||
| + | </a> | ||
| + | <div class="thumbcaption"> | ||
| + | <div class="magnify"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/3/3f/110.112.113.jpg" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>GEL : 1% agarose gel. PCR product of 1 colony of the PSB1C3+E0240+J23104, PSB1C3+E0240+J23112 and PSB1C3+E0240+J23113 constructions. </center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | </ul> | ||
| + | |||
| + | A pre-culture was made for each colony to make a glycerol stock.<br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | |||
Latest revision as of 16:57, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23100:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
OVERFLOW
PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED
PSB1C3+E0240+J23103:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).
PSB1C3+E0240+J23105:
pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
PSB1C3+E0240+J23107:
pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.
PSB1C3+E0240+J23110:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (12h-19h).
PSB1C3+E0240+J23104, PSB1C3+E0240+J23112, PSB1C3+E0240+J23113: PCR colony of 6 colonies for each construction following protocol table 1 and 2.
Migration of the PCR products
PCR purification of clones with the good construction
Sep 17
