Team:Evry/Interlab Study/09-14-2014

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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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<i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>:  
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<i>PSB1C3+E0240+J23101</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200.<br>
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<FONT color="red">TECAN FAILED</FONT> <br>
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<br>
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<i>PSB1C3+E0240+J23106</i>:<br>
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pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C.
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<ul>
 
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        <li> PCR colony of 3 colonies for each construction following protocol table 1 and 2.
 
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https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg
 
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<div class="center">
 
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<div class="thumb tnone">
 
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  <div class="thumbinner" style="width:502px;">
 
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  <a href="https://static.igem.org/mediawiki/2014/8/8f/Gel106_107gfp.jpg" class="image">
 
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/8/8f/Gel106_107gfp.jpg" width="450px;" class="thumbimage"/>
 
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  </a>
 
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  <div class="thumbcaption">
 
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    <div class="magnify">
 
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    <a href="https://static.igem.org/mediawiki/2014/8/8f/Gel106_107gfp.jpg" class="internal" title="Enlarge">
 
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      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
 
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    </a>
 
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    </div>
 
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    <center>GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction. </center>
 
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  </div>
 
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  </div>
 
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</div>
 
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</div>
 
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<div class="center">
 
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<div class="thumb tnone">
 
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  <div class="thumbinner" style="width:502px;">
 
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  <a href="https://static.igem.org/mediawiki/2014/c/c9/Gel105gfp.jpg" class="image">
 
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/c/c9/Gel105gfp.jpg" width="450px;" class="thumbimage"/>
 
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  </a>
 
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  <div class="thumbcaption">
 
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    <div class="magnify">
 
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    <a href="https://static.igem.org/mediawiki/2014/c/c9/Gel105gfp.jpg" class="internal" title="Enlarge">
 
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      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
 
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    </a>
 
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    </div>
 
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    <center>GEL 3: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.</center>
 
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  </div>
 
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  </div>
 
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</div>
 
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</div>
 
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      </ul>
 

Latest revision as of 22:59, 16 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010



Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED

PSB1C3+E0240+J23106:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C.

Sep 14

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