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Team:Evry/Interlab Study/09-11-2014
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<h2>Other constructions of the Anderson library of constitutive promoters</h2> | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
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| - | PSB1C3+E0240+J23101, <i>PSB1C3+E0240+J23118</i>, <i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>: | + | <i>PSB1C3+E0240+J23101</i>, <i>PSB1C3+E0240+J23118</i>, <i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>: |
<ul> | <ul> | ||
| - | <li> Transformation plate from the 10th September observation. There were 50 colonies for each constructions. | + | <li> Transformation plate from the 10th September observation. There were 50 colonies for each constructions.</ul> |
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| + | <i>PSB1C3+E0240+J23101</i> and <i>PSB1C3+E0240+J23118</i>,<br> | ||
| + | <ul> | ||
| + | <li> PCR colony of 3 colonies for each construction following protocol table 1 and 2. | ||
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</a> | </a> | ||
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| - | <center>1% agarose gel. Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction. Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center> | + | <center>GEL 1: 1% agarose gel.<br> Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.<br> Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. </center> |
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| + | <li> PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000. | ||
| + | <li> Preparation of samples to sequencing. N° XX | ||
| + | <li> Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C. | ||
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| - | + | <i>PSB1C3+E0240+J23100</i>, <i>PSB1C3+E0240+J23102</i>, <i>PSB1C3+E0240+J23103</i>, <i>PSB1C3+E0240+J23104</i> and <i>PSB1C3+E0240+J23118</i>,<br> | |
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| - | + | <li> PCR colony of 1 colony for each construction following protocol table 1 and 2. | |
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| - | <a href="https://static.igem.org/mediawiki/2014/c/ | + | <a href="https://static.igem.org/mediawiki/2014/c/c1/Gel11092014.jpg" class="image"> |
| - | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/ | + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/f/f7/Gel_11.jpg" width="450px;" class="thumbimage"/> |
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| - | <a href="https://static.igem.org/mediawiki/2014/ | + | <a href="https://static.igem.org/mediawiki/2014/f/f7/Gel_11.jpg" class="internal" title="Enlarge"> |
<img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
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| - | <center>1% agarose gel. PCR product of | + | <center>GEL 2: 1% agarose gel.<br> Lane 1: PCR product of 1 colonies of the PSB1C3+E0240+J23100 construction.<br> Lane 2: PCR product of 1 colonies of the PSB1C3+E0240+J23102 construction.<br> Lane 3: PCR product of 1 colonies of the PSB1C3+E0240+J23103 construction.<br> Lane 4: PCR product of 1 colonies of the PSB1C3+E0240+J23104 construction. </center> |
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| + | A pre-culture was made for each colony to make a glycerol stock.<br> | ||
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<span class="cd-date">Sep 11</span> | <span class="cd-date">Sep 11</span> | ||
</div> | </div> | ||
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</html> | </html> | ||
Latest revision as of 18:55, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101, PSB1C3+E0240+J23118, PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
- Transformation plate from the 10th September observation. There were 50 colonies for each constructions.
PSB1C3+E0240+J23101 and PSB1C3+E0240+J23118,
- PCR colony of 3 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel.
Lane 1 to 3: PCR product of 3 colonies of the PSB1C3+E0240+J23101 construction.
Lane 4 to 6: PCR product of 3 colonies of the PSB1C3+E0240+J23118 construction. - PCR purification of sample 1 and 4 of the Gel 1 were purified with the NucleoSpin kit (Macherey Nagel). DNA was quantify by Nanodrop 2000.
- Preparation of samples to sequencing. N° XX
- Preparation of 3 ml cultures LB Cam. Incubation overnight at 37°C.
PSB1C3+E0240+J23100, PSB1C3+E0240+J23102, PSB1C3+E0240+J23103, PSB1C3+E0240+J23104 and PSB1C3+E0240+J23118,
- PCR colony of 1 colony for each construction following protocol table 1 and 2.
GEL 2: 1% agarose gel.
Lane 1: PCR product of 1 colonies of the PSB1C3+E0240+J23100 construction.
Lane 2: PCR product of 1 colonies of the PSB1C3+E0240+J23102 construction.
Lane 3: PCR product of 1 colonies of the PSB1C3+E0240+J23103 construction.
Lane 4: PCR product of 1 colonies of the PSB1C3+E0240+J23104 construction.
A pre-culture was made for each colony to make a glycerol stock.
Sep 11
